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The map label for this gene is rlmN

Identifier: 21674009

GI number: 21674009

Start: 1112492

End: 1113616

Strand: Direct

Name: rlmN

Synonym: CT1183

Alternate gene names: 21674009

Gene position: 1112492-1113616 (Clockwise)

Preceding gene: 21674005

Following gene: 21674010

Centisome position: 51.63

GC content: 50.4

Gene sequence:

>1125_bases
ATGGAAAAAAATGAAATATCAGAAGAGAGGCGGACCCAAGAAAAGGAAAAACAGCATGGCCACCGTCTGAACATCAGAAG
ACTGGGCAGAAAGGAACTTACCGAACTCTTGACCCGTCTCGGAGAGCCAGCTTATCGCGCAAATCAGCTCCATCGATGGC
TGTACAGTAATCAGGCCCTCCGTTTTGAGGAAATGAGCACCCTCAGCAAACAACTGCGTCAGAAACTCGCTTCTGAATGG
ATTATACATCCAGCCTCACTTGTCGGCACCGAACGCGAAACGACGGACGCCAGTTTGGTGACCGGAAATCCAACGGCAAA
ATTCCTCATCAAGCTGGAGGATAACGAACTGGTCGAAAGTGTTCTCATCCCTTCGGAAGAGCGGATAACTGCCTGCATCT
CCTCGCAAATCGGCTGCCCGCTCCGCTGTACCTTCTGCGCAACAGGACACATGGGATTCAGAAGAAACCTCACGGCATCG
GAAATAACCGATCAGGTATTTCTGCTTGAAAAAGAAGCGCAGAAGCGCCACTGGCGGGGGCTGACCAATATTGTCTTCAT
GGGCATGGGCGAACCGTTGCTGAATCTTGACAATGTACTTGAGTCGATCGGAACGCTGACTGAAAAGGATTACCAATTCA
GCATCTCCGAAAGAAAGATCACGATTTCAACCGTCGGTTTGCCCGTGGAAATGGACAGAATCGCCCGGTCGGGACTCAAA
ACCAAACTCGCCATATCGCTGCACAGCGCCGACCAGCTCATACGGGAACGGATGATGCCAATCGCCGCTGACATTACCCT
CGACAAACTCGCCAAAGCGATAAACTCTTATAACTCCGTAACCAGCCAACCAGTTACGCTTGTCTACATGCTGCTGGAAG
GCATCAACGATTCGCCTGAAGATGCCCGCAAACTGGTTCGTTTCGCAAAGAGAGTGTTGTGCAAAATTAATTTGATTGAT
TATAATTCTATCGTTACCTTGAAGTTCAAGCCGGGTTGCAGTAGCTCGAAAACCATGTTCATTCAACAGTTACTGGATGC
CGGGCTGCTCGTCACCGTCAGAAAAAGTCAGGGTGCAACCATCAACGCAGCATGCGGACAGCTGGCCACCCGGCCTGTGA
GGTAA

Upstream 100 bases:

>100_bases
TTCTTTCTTTTAAATTATTTCAACGGTTACTAAGGAGACTTGACGTCGTATACAAATAAGAGTACTTTTTTTTTCGATCA
AGAGCAAGCGAAGCAACAAA

Downstream 100 bases:

>100_bases
TAACGGTTTGAAACCGAGAAGAAATCTTTAACCGGCCCATCTCATGGATTTATCCTCTTTCCTTCCCTTCAGAGACGAAA
TGGTCAAGGTATATCACTGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MEKNEISEERRTQEKEKQHGHRLNIRRLGRKELTELLTRLGEPAYRANQLHRWLYSNQALRFEEMSTLSKQLRQKLASEW
IIHPASLVGTERETTDASLVTGNPTAKFLIKLEDNELVESVLIPSEERITACISSQIGCPLRCTFCATGHMGFRRNLTAS
EITDQVFLLEKEAQKRHWRGLTNIVFMGMGEPLLNLDNVLESIGTLTEKDYQFSISERKITISTVGLPVEMDRIARSGLK
TKLAISLHSADQLIRERMMPIAADITLDKLAKAINSYNSVTSQPVTLVYMLLEGINDSPEDARKLVRFAKRVLCKINLID
YNSIVTLKFKPGCSSSKTMFIQQLLDAGLLVTVRKSQGATINAACGQLATRPVR

Sequences:

>Translated_374_residues
MEKNEISEERRTQEKEKQHGHRLNIRRLGRKELTELLTRLGEPAYRANQLHRWLYSNQALRFEEMSTLSKQLRQKLASEW
IIHPASLVGTERETTDASLVTGNPTAKFLIKLEDNELVESVLIPSEERITACISSQIGCPLRCTFCATGHMGFRRNLTAS
EITDQVFLLEKEAQKRHWRGLTNIVFMGMGEPLLNLDNVLESIGTLTEKDYQFSISERKITISTVGLPVEMDRIARSGLK
TKLAISLHSADQLIRERMMPIAADITLDKLAKAINSYNSVTSQPVTLVYMLLEGINDSPEDARKLVRFAKRVLCKINLID
YNSIVTLKFKPGCSSSKTMFIQQLLDAGLLVTVRKSQGATINAACGQLATRPVR
>Mature_374_residues
MEKNEISEERRTQEKEKQHGHRLNIRRLGRKELTELLTRLGEPAYRANQLHRWLYSNQALRFEEMSTLSKQLRQKLASEW
IIHPASLVGTERETTDASLVTGNPTAKFLIKLEDNELVESVLIPSEERITACISSQIGCPLRCTFCATGHMGFRRNLTAS
EITDQVFLLEKEAQKRHWRGLTNIVFMGMGEPLLNLDNVLESIGTLTEKDYQFSISERKITISTVGLPVEMDRIARSGLK
TKLAISLHSADQLIRERMMPIAADITLDKLAKAINSYNSVTSQPVTLVYMLLEGINDSPEDARKLVRFAKRVLCKINLID
YNSIVTLKFKPGCSSSKTMFIQQLLDAGLLVTVRKSQGATINAACGQLATRPVR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=370, Percent_Identity=35.4054054054054, Blast_Score=228, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CHLTE (Q8KD71)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662074.1
- ProteinModelPortal:   Q8KD71
- GeneID:   1006813
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1183
- NMPDR:   fig|194439.1.peg.1168
- TIGR:   CT1183
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14456
- BioCyc:   CTEP194439:CT_1183-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42198; Mature: 42198

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNEISEERRTQEKEKQHGHRLNIRRLGRKELTELLTRLGEPAYRANQLHRWLYSNQAL
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
RFEEMSTLSKQLRQKLASEWIIHPASLVGTERETTDASLVTGNPTAKFLIKLEDNELVES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCCEEEEEEECCHHHHHH
VLIPSEERITACISSQIGCPLRCTFCATGHMGFRRNLTASEITDQVFLLEKEAQKRHWRG
HHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LTNIVFMGMGEPLLNLDNVLESIGTLTEKDYQFSISERKITISTVGLPVEMDRIARSGLK
HHHEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCC
TKLAISLHSADQLIRERMMPIAADITLDKLAKAINSYNSVTSQPVTLVYMLLEGINDSPE
EEEEEEEHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
DARKLVRFAKRVLCKINLIDYNSIVTLKFKPGCSSSKTMFIQQLLDAGLLVTVRKSQGAT
HHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCE
INAACGQLATRPVR
EEHHHHHHHCCCCC
>Mature Secondary Structure
MEKNEISEERRTQEKEKQHGHRLNIRRLGRKELTELLTRLGEPAYRANQLHRWLYSNQAL
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
RFEEMSTLSKQLRQKLASEWIIHPASLVGTERETTDASLVTGNPTAKFLIKLEDNELVES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCCEEEEEEECCHHHHHH
VLIPSEERITACISSQIGCPLRCTFCATGHMGFRRNLTASEITDQVFLLEKEAQKRHWRG
HHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LTNIVFMGMGEPLLNLDNVLESIGTLTEKDYQFSISERKITISTVGLPVEMDRIARSGLK
HHHEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCC
TKLAISLHSADQLIRERMMPIAADITLDKLAKAINSYNSVTSQPVTLVYMLLEGINDSPE
EEEEEEEHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
DARKLVRFAKRVLCKINLIDYNSIVTLKFKPGCSSSKTMFIQQLLDAGLLVTVRKSQGAT
HHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCE
INAACGQLATRPVR
EEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901