Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 21673990

GI number: 21673990

Start: 1095495

End: 1096187

Strand: Reverse

Name: trmD

Synonym: CT1164

Alternate gene names: 21673990

Gene position: 1096187-1095495 (Counterclockwise)

Preceding gene: 21673991

Following gene: 21673989

Centisome position: 50.87

GC content: 55.41

Gene sequence:

>693_bases
ATGCGGATAGAGATCATTTCCGTCATTCCGGATTTTTTTGCATCACCTCTCGAAAAGGGATTGCTTGGTATCGCAAGACG
GAAAGGTCTCGCTGAGATCCATGTGCACAATCTGCACGATTACGGATTGGGCAAGTACCAGCAGGTGGATGATGCGCCGT
TCGGAGGTGGCGCCGGAATGGTGATTCGTCCCGAGCCGGTTTTCGCGTGTATCGAGAAGTTGCAGGCTGAAAGGAGTTAT
GACGAGGTGATCTTCATGACGCCTGACGGGCAGGCTTTCGACCAGAAGCGGGCAAACCGCCTTTCGCGAAAGGGGAATCT
GATCATTCTCTGTGGACACTACAAGGCCATAGATGAAAGGATTCGCCGGACGCTGGTCACCATGGAGCTTTCCGTCGGCG
ATGTGGTGCTTTCCGGAGGTGAAATTCCGGCGCTCATGGTCATGGATGCGGTGCTCAGGCTCATCCCGGGTGTGCTTGGC
GATGGTGAATCGGCGCTGACCGATTCGTTCCAGAACGAGCTGCTCGACTGCGCATGGTACACCCGTCCGGCGGAGTTCCG
GGGAATGAAGGTGCCCGATGTGCTTCTTTCAGGCCATCATGAAAAAATCGAGCTTTGGCGGCAGGAGAATGCGCGTGAAC
GCACGCTTGAGCGCAGGCCGGAGATGCTCGGCAAAGAGAGTGAAAAAGAGTAA

Upstream 100 bases:

>100_bases
CAAAGAATTTTTGTAACGTCACTGCGTTGCGGCTTCTTTTTTGAAGTTTTGGCGCAATGTTCTTGACAAGATTGACAGGC
AACGACTCAGGTGTGAAGCC

Downstream 100 bases:

>100_bases
AGACAACATAAAGATAACAGTGTCATCATGGATCAGTTAATTCAGTTGGTCGAAGCAACCCAGCAGAGGAACGATATTCC
CGAGGTTCGTCCTGGCGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRIEIISVIPDFFASPLEKGLLGIARRKGLAEIHVHNLHDYGLGKYQQVDDAPFGGGAGMVIRPEPVFACIEKLQAERSY
DEVIFMTPDGQAFDQKRANRLSRKGNLIILCGHYKAIDERIRRTLVTMELSVGDVVLSGGEIPALMVMDAVLRLIPGVLG
DGESALTDSFQNELLDCAWYTRPAEFRGMKVPDVLLSGHHEKIELWRQENARERTLERRPEMLGKESEKE

Sequences:

>Translated_230_residues
MRIEIISVIPDFFASPLEKGLLGIARRKGLAEIHVHNLHDYGLGKYQQVDDAPFGGGAGMVIRPEPVFACIEKLQAERSY
DEVIFMTPDGQAFDQKRANRLSRKGNLIILCGHYKAIDERIRRTLVTMELSVGDVVLSGGEIPALMVMDAVLRLIPGVLG
DGESALTDSFQNELLDCAWYTRPAEFRGMKVPDVLLSGHHEKIELWRQENARERTLERRPEMLGKESEKE
>Mature_230_residues
MRIEIISVIPDFFASPLEKGLLGIARRKGLAEIHVHNLHDYGLGKYQQVDDAPFGGGAGMVIRPEPVFACIEKLQAERSY
DEVIFMTPDGQAFDQKRANRLSRKGNLIILCGHYKAIDERIRRTLVTMELSVGDVVLSGGEIPALMVMDAVLRLIPGVLG
DGESALTDSFQNELLDCAWYTRPAEFRGMKVPDVLLSGHHEKIELWRQENARERTLERRPEMLGKESEKE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=43.3035714285714, Blast_Score=180, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLTE (Q8KD90)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662055.1
- ProteinModelPortal:   Q8KD90
- SMR:   Q8KD90
- GeneID:   1006527
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1164
- NMPDR:   fig|194439.1.peg.1149
- TIGR:   CT1164
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   CTEP194439:CT_1164-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25870; Mature: 25870

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEIISVIPDFFASPLEKGLLGIARRKGLAEIHVHNLHDYGLGKYQQVDDAPFGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VIRPEPVFACIEKLQAERSYDEVIFMTPDGQAFDQKRANRLSRKGNLIILCGHYKAIDER
EECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHH
IRRTLVTMELSVGDVVLSGGEIPALMVMDAVLRLIPGVLGDGESALTDSFQNELLDCAWY
HHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TRPAEFRGMKVPDVLLSGHHEKIELWRQENARERTLERRPEMLGKESEKE
CCCHHHCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIEIISVIPDFFASPLEKGLLGIARRKGLAEIHVHNLHDYGLGKYQQVDDAPFGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VIRPEPVFACIEKLQAERSYDEVIFMTPDGQAFDQKRANRLSRKGNLIILCGHYKAIDER
EECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHH
IRRTLVTMELSVGDVVLSGGEIPALMVMDAVLRLIPGVLGDGESALTDSFQNELLDCAWY
HHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TRPAEFRGMKVPDVLLSGHHEKIELWRQENARERTLERRPEMLGKESEKE
CCCHHHCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901