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The map label for this gene is pleD [H]

Identifier: 21673931

GI number: 21673931

Start: 1039996

End: 1041207

Strand: Reverse

Name: pleD [H]

Synonym: CT1105

Alternate gene names: 21673931

Gene position: 1041207-1039996 (Counterclockwise)

Preceding gene: 21673932

Following gene: 21673929

Centisome position: 48.32

GC content: 52.72

Gene sequence:

>1212_bases
ATGATTTTCGGTATTGTGGCGCTGATTCTGGCTGCCGGAGGAACCGCCTGGTTTGGTGCTTTCCAGAGTTCCCGTCTTCA
CCAGATCAGTGAAGACGAGTCGTTGCGCATGAGGCTGGTTGAATGCCGCACCAGCATTTCGTCGATCGAACAGCTTTTTG
GGGATGAGCATCTTGTACTTGGCAAACAGGCGGATATTCAGTCGAAGCTCCGGATCGAGTGTGCCAGGTTTTCGGAGATT
GGGCGCAGCCTGGAGATGAAGCAGGGAAGTGAACTCAACCAGTGGCTGAAACACCGCCAGCCGCTTGCCGCTCCGGTCAG
CCGCGAATCGCTCCAGCTCATGGTCGTCGATATGGATGGCATGATAGAAAAGCTGAGCGCGAAGATCACTGATGAAAAAG
CGCAGCTCACAGCCAGCCTGAATTACTATCTCGTGTTTATTACGCTTTTGCTGGTTGTTGTTGTGCTGGCTGGCGGGAAA
CTTATCATCTCCAGCTACCGGCGCTCGATCATTCCACTCCATAAACTTTCAGAACGGCTTGCCCTGTTGAATTGCAATCT
TTCCGAAAGTTTGCACGATACCGCCGAAGCTGCTGAAACCCTTTTGAACGGCGAGGATCCATCCGCCGACATGCGCTCCG
TCAGCGAATCGGTCGCGAGCATGTGCCACGACATCGAGGAGAAAAACCGCAAGCTTGACGAGTTGCACATCCGCGATGAA
AAAACCAATCTTTACAACTATCGCCACTTCAAGGAGCATCTCATTATCGATGTCGAGCGGGCACGGCGCTTCAACGGCGA
TATCTCGCTCGCCATGATCGATATCGACAACTTCAAGAACTATAACGACCGTTACGGGCACGTTGCCGGAGACAGGGCGC
TGGCGAGAATCGCCGAAATCATCAGGAAGGAGTGTCGCATGACCGATATTCCATCGAGGTTTGGGGGTGACGAATTCGCC
ATCCTTTTTCCGAAGACTGACCGGGCGACAGCGCTTGATATTTCCGAGCGGCTGCGCAACATCATTTACGCAGAACCGTT
CGAGCACGAAAGCAAGCAGCCCGGCGGACAACTGACGGTGAGTATCGGTGTCGCCTCATGGCTCGATGACGCCACTGACG
GTACTTCGCTGATTGTGAATGCTGACAAAGCTCTTTACAAAGCAAAGTCATCAGGGAAAAATATGGTTTTGGCATTTACG
AGGGAGGTTTAA

Upstream 100 bases:

>100_bases
GACATGGCGCCATACGAACAGCTCGTGCGTACCATCATTCTCAAGTGACTAAATGAAAAACGAAAGCGATACTACAGGCA
ATTACTTCCTCAGGTTAACA

Downstream 100 bases:

>100_bases
AAGAGAAGGTGGCGGAGCGGTAGTGGGGAATAACCCGGCTTGGCTTGCGGGTCACTGCCCTTACAATCCGGATTTCCGAC
ATCCCTTGAATCACCTTTTG

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MIFGIVALILAAGGTAWFGAFQSSRLHQISEDESLRMRLVECRTSISSIEQLFGDEHLVLGKQADIQSKLRIECARFSEI
GRSLEMKQGSELNQWLKHRQPLAAPVSRESLQLMVVDMDGMIEKLSAKITDEKAQLTASLNYYLVFITLLLVVVVLAGGK
LIISSYRRSIIPLHKLSERLALLNCNLSESLHDTAEAAETLLNGEDPSADMRSVSESVASMCHDIEEKNRKLDELHIRDE
KTNLYNYRHFKEHLIIDVERARRFNGDISLAMIDIDNFKNYNDRYGHVAGDRALARIAEIIRKECRMTDIPSRFGGDEFA
ILFPKTDRATALDISERLRNIIYAEPFEHESKQPGGQLTVSIGVASWLDDATDGTSLIVNADKALYKAKSSGKNMVLAFT
REV

Sequences:

>Translated_403_residues
MIFGIVALILAAGGTAWFGAFQSSRLHQISEDESLRMRLVECRTSISSIEQLFGDEHLVLGKQADIQSKLRIECARFSEI
GRSLEMKQGSELNQWLKHRQPLAAPVSRESLQLMVVDMDGMIEKLSAKITDEKAQLTASLNYYLVFITLLLVVVVLAGGK
LIISSYRRSIIPLHKLSERLALLNCNLSESLHDTAEAAETLLNGEDPSADMRSVSESVASMCHDIEEKNRKLDELHIRDE
KTNLYNYRHFKEHLIIDVERARRFNGDISLAMIDIDNFKNYNDRYGHVAGDRALARIAEIIRKECRMTDIPSRFGGDEFA
ILFPKTDRATALDISERLRNIIYAEPFEHESKQPGGQLTVSIGVASWLDDATDGTSLIVNADKALYKAKSSGKNMVLAFT
REV
>Mature_403_residues
MIFGIVALILAAGGTAWFGAFQSSRLHQISEDESLRMRLVECRTSISSIEQLFGDEHLVLGKQADIQSKLRIECARFSEI
GRSLEMKQGSELNQWLKHRQPLAAPVSRESLQLMVVDMDGMIEKLSAKITDEKAQLTASLNYYLVFITLLLVVVVLAGGK
LIISSYRRSIIPLHKLSERLALLNCNLSESLHDTAEAAETLLNGEDPSADMRSVSESVASMCHDIEEKNRKLDELHIRDE
KTNLYNYRHFKEHLIIDVERARRFNGDISLAMIDIDNFKNYNDRYGHVAGDRALARIAEIIRKECRMTDIPSRFGGDEFA
ILFPKTDRATALDISERLRNIIYAEPFEHESKQPGGQLTVSIGVASWLDDATDGTSLIVNADKALYKAKSSGKNMVLAFT
REV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=34.4827586206897, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=32.5966850828729, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=30.939226519337, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=36.3157894736842, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=37.4045801526718, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082007, Length=132, Percent_Identity=35.6060606060606, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=32.9192546583851, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=28.021978021978, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=31.0160427807487, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787056, Length=131, Percent_Identity=33.587786259542, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45287; Mature: 45287

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFGIVALILAAGGTAWFGAFQSSRLHQISEDESLRMRLVECRTSISSIEQLFGDEHLVL
CHHHHHHHHHHCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GKQADIQSKLRIECARFSEIGRSLEMKQGSELNQWLKHRQPLAAPVSRESLQLMVVDMDG
CCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECHHH
MIEKLSAKITDEKAQLTASLNYYLVFITLLLVVVVLAGGKLIISSYRRSIIPLHKLSERL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
ALLNCNLSESLHDTAEAAETLLNGEDPSADMRSVSESVASMCHDIEEKNRKLDELHIRDE
HHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECC
KTNLYNYRHFKEHLIIDVERARRFNGDISLAMIDIDNFKNYNDRYGHVAGDRALARIAEI
CCCHHHHHHHHHHHEEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
IRKECRMTDIPSRFGGDEFAILFPKTDRATALDISERLRNIIYAEPFEHESKQPGGQLTV
HHHHCCCCCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCEEEE
SIGVASWLDDATDGTSLIVNADKALYKAKSSGKNMVLAFTREV
EEEHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure
MIFGIVALILAAGGTAWFGAFQSSRLHQISEDESLRMRLVECRTSISSIEQLFGDEHLVL
CHHHHHHHHHHCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GKQADIQSKLRIECARFSEIGRSLEMKQGSELNQWLKHRQPLAAPVSRESLQLMVVDMDG
CCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECHHH
MIEKLSAKITDEKAQLTASLNYYLVFITLLLVVVVLAGGKLIISSYRRSIIPLHKLSERL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
ALLNCNLSESLHDTAEAAETLLNGEDPSADMRSVSESVASMCHDIEEKNRKLDELHIRDE
HHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECC
KTNLYNYRHFKEHLIIDVERARRFNGDISLAMIDIDNFKNYNDRYGHVAGDRALARIAEI
CCCHHHHHHHHHHHEEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
IRKECRMTDIPSRFGGDEFAILFPKTDRATALDISERLRNIIYAEPFEHESKQPGGQLTV
HHHHCCCCCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCEEEE
SIGVASWLDDATDGTSLIVNADKALYKAKSSGKNMVLAFTREV
EEEHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA