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The map label for this gene is purB [H]

Identifier: 21673817

GI number: 21673817

Start: 937157

End: 938461

Strand: Reverse

Name: purB [H]

Synonym: CT0989

Alternate gene names: 21673817

Gene position: 938461-937157 (Counterclockwise)

Preceding gene: 21673818

Following gene: 21673814

Centisome position: 43.55

GC content: 58.7

Gene sequence:

>1305_bases
GTGATACCACGTTACTCGCCGAAAGACATCTCGGCAATCTGGAGCGATGAAGCCAAATTCGAACGCTGGCTGCAACTCGA
AATCGCTGCCGTCGAGGCACGCATGGAAGCCGGCATCGTTCCGGCGGACGCCCTTGCGACCATTAAAGAAAAAGCCAGGT
TCAACGTCGAAGAGATTCTTATCATTGAAAACGAGACGAAGCACGACGTCATTGCCTTTCTAACCAACGTCGCCGGCTAC
GTCGGCCCCGAATCACGTTACATCCACGAGGGTCTCACCTCTTCGGACGTGGTCGATACCTGCCTGGCGATGCAGATGCG
CGATGCGGGCAAGATCATCGTGGCTGACATCGAGTCGCTTATCGAGGTGCTCGGCAAAAAGGCCGTCGAACACAAATACA
CCCTTCAGATGGGCCGCACGCACGGCATTCACGCCGAGCCGACCACGTTCGGCCTGAAGCTGTTGCTCTGGTACGAGGAG
ATGAAGCGCAACCTCGAACGCATGAAGCGCGCGCTCGAAACCGTCTCGGTCGGTAAAATTTCCGGCGCGGTCGGCACCTA
CCAGCATCTGTCACCGGACATCGAGGCCGCCGTCTGCCAGAAGCTTGGCCTGAAGCCCTCGTCGATCTCGACGCAGATTC
TCCAGCGCGACCGACACGCCGAATACGCCACGACGCTCGCCATCGTCGCCTCGTCCATCGAGAAGTTCTCGACCGAACTG
CGCCACCTGCAACGCACCGAGGTTCGCGAAACCGAGGAGTTTTTCAGCAAGGGCCAGAAAGGCAGCTCCGCCATGCCGCA
CAAGCGCAACCCGATCACCTTTGAGCGCCTCACCGGCTTGGCTCGCGTGGTGCGCTCCAACTCCATCGCCGCCATGGAGA
ACGTCGCCCTCTGGCACGAACGCGACATTTCGCACTCCTCGGTCGAGCGCGTCATCATGCCCGACTCGACCATCGCGCTG
GTTTATATGCTGCGCACCTTCCGTGATTCGATCGAGACCTTGCTCGTCTATCCGGAGCGCATGAAGCAAAACTTCGACAC
CTCCTACGGCCTGACCCTCTCGCAGACGCTGCTGCTCGCCCTCACCGGCAAGGGACTCACCCGTGAAGACGCCTACCGCC
TCGTGCAGCGCAACGCCATGAAAAGCTGGGAGGAGAAAATCCAGCTCAAGGAGCTGGTGATTCACGACCCGGAAATCCTC
GAACACATCAGCGCAGAAGAGATCAACCAGCTCTTCAGCCCGGAGACAATTCAGGACAAGCTCAAAAACAGCGTGGATAT
TATTTTCAAGCGCAACGGGCTGTGA

Upstream 100 bases:

>100_bases
TTTTTCGCTCTTCTCCTTGCAGGCCATCTTCTCCATGCCTTTGAAAGGCCAAGAACTATATTCATTACTACCGGAAGCTT
AAAACCAAAACCGAACCACA

Downstream 100 bases:

>100_bases
GCTGAGGAAACTCAAAATTTTCACCCCCGGCTTGAAAGCCGGGGCAATGATAACTATAAGAGTGAGAATGTCTAAAGGGT
TAAAACCCTTCTGAATGCAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPADALATIKEKARFNVEEILIIENETKHDVIAFLTNVAGY
VGPESRYIHEGLTSSDVVDTCLAMQMRDAGKIIVADIESLIEVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWYEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCQKLGLKPSSISTQILQRDRHAEYATTLAIVASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMKQNFDTSYGLTLSQTLLLALTGKGLTREDAYRLVQRNAMKSWEEKIQLKELVIHDPEIL
EHISAEEINQLFSPETIQDKLKNSVDIIFKRNGL

Sequences:

>Translated_434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPADALATIKEKARFNVEEILIIENETKHDVIAFLTNVAGY
VGPESRYIHEGLTSSDVVDTCLAMQMRDAGKIIVADIESLIEVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWYEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCQKLGLKPSSISTQILQRDRHAEYATTLAIVASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMKQNFDTSYGLTLSQTLLLALTGKGLTREDAYRLVQRNAMKSWEEKIQLKELVIHDPEIL
EHISAEEINQLFSPETIQDKLKNSVDIIFKRNGL
>Mature_434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPADALATIKEKARFNVEEILIIENETKHDVIAFLTNVAGY
VGPESRYIHEGLTSSDVVDTCLAMQMRDAGKIIVADIESLIEVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWYEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCQKLGLKPSSISTQILQRDRHAEYATTLAIVASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMKQNFDTSYGLTLSQTLLLALTGKGLTREDAYRLVQRNAMKSWEEKIQLKELVIHDPEIL
EHISAEEINQLFSPETIQDKLKNSVDIIFKRNGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=384, Percent_Identity=27.0833333333333, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=27.0341207349081, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787376, Length=349, Percent_Identity=30.0859598853868, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=414, Percent_Identity=26.5700483091787, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=360, Percent_Identity=28.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=451, Percent_Identity=25.9423503325942, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=27.8947368421053, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49192; Mature: 49192

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPADALATIKEKARFNVEEIL
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
IIENETKHDVIAFLTNVAGYVGPESRYIHEGLTSSDVVDTCLAMQMRDAGKIIVADIESL
EEECCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHH
IEVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWYEEMKRNLERMKRALETVSVGKI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPDIEAAVCQKLGLKPSSISTQILQRDRHAEYATTLAIVASSIEKFSTEL
CCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTIALVYMLRTFRDSIETLLVYPERMKQNFDTSYGLTLSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
LTGKGLTREDAYRLVQRNAMKSWEEKIQLKELVIHDPEILEHISAEEINQLFSPETIQDK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCHHHHHH
LKNSVDIIFKRNGL
HCCCEEEEEECCCC
>Mature Secondary Structure
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPADALATIKEKARFNVEEIL
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
IIENETKHDVIAFLTNVAGYVGPESRYIHEGLTSSDVVDTCLAMQMRDAGKIIVADIESL
EEECCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHH
IEVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWYEEMKRNLERMKRALETVSVGKI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPDIEAAVCQKLGLKPSSISTQILQRDRHAEYATTLAIVASSIEKFSTEL
CCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTIALVYMLRTFRDSIETLLVYPERMKQNFDTSYGLTLSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
LTGKGLTREDAYRLVQRNAMKSWEEKIQLKELVIHDPEILEHISAEEINQLFSPETIQDK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCHHHHHH
LKNSVDIIFKRNGL
HCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]