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The map label for this gene is truA

Identifier: 21673806

GI number: 21673806

Start: 925437

End: 926180

Strand: Direct

Name: truA

Synonym: CT0978

Alternate gene names: 21673806

Gene position: 925437-926180 (Clockwise)

Preceding gene: 21673799

Following gene: 21673807

Centisome position: 42.94

GC content: 61.69

Gene sequence:

>744_bases
ATGGCGAGATCGAAAAGAACCATCAAAATGCAGATCGAGTATGACGGCACCGGCTATTCGGGGTGGCAGCGGCAGCCGGG
TGACGTAGTGACCGTGCAAGGCGAGATCGAGCGCGTGCTCGAACGCATCATGCAGGAACCGGTGAGCATCGATGGCGCAG
GCCGAACCGACAGTGGAGTGCACGCGCGCAGGCAGGTAGCGAGCTTTGCGACGTGCTCGCCGATGCCGCTCGGCCGCCTG
ATATACTCGGCCAATTCGCTTTTGCCCTCGACGATCCGCATCAACGCCATGCGGCAGGCACCGGAGTCGTTTCACGCCCG
CTTTAGTGCCACGTCGAGGGAGTACCGCTACTTTTTGCTCGAACATCCTTCAGCTATCGACAGCCGCTTCGCCGGATGCT
CGCACGGCAAGCCGGACGTCGGCGCGATGAACCGGCTCGCATTGATGCTCATCGGAACGCACGATTTCGCAGCCTTTTCG
AAAGAGACCCCCGACCAGTACGGGACGCTCTGTACGGTTACGGCAGCGCGCTGGTACCGTTCCGGTCGGTTCCACGTTTT
TCGCATCGAGGCGAACCGCTTTCTGCGGAGCATGGTGCGCTTTCTGGTGGCCGGGATGATCGAGGTTGGAATGGGCCGAC
TCGAAGAAGGGGCGTTCGCCCGGATGCTCGAAAGCGGCCATCGCCCGCCAAAGCTGAAGCCTGCCGACGCGGCGGGACTG
TTCCTCTGGAAGGTCAGGTATTGA

Upstream 100 bases:

>100_bases
CTGTCAGATATTCTGAATCGATGGAAGCGAAAAATCAGGCTAAAGATAAGTATTATCGGGAAAAGATATGCAGAACCAAT
CAAGGCATTTGTGACGCAGT

Downstream 100 bases:

>100_bases
AATAAATTATGAAATTATGAGTGATGAATGATGCATTGGTATGGGAAGGGGCTGCCGAGAGATTTTCGAGCGGGGTGATT
CCGGAACTCCTTTTAATGGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MARSKRTIKMQIEYDGTGYSGWQRQPGDVVTVQGEIERVLERIMQEPVSIDGAGRTDSGVHARRQVASFATCSPMPLGRL
IYSANSLLPSTIRINAMRQAPESFHARFSATSREYRYFLLEHPSAIDSRFAGCSHGKPDVGAMNRLALMLIGTHDFAAFS
KETPDQYGTLCTVTAARWYRSGRFHVFRIEANRFLRSMVRFLVAGMIEVGMGRLEEGAFARMLESGHRPPKLKPADAAGL
FLWKVRY

Sequences:

>Translated_247_residues
MARSKRTIKMQIEYDGTGYSGWQRQPGDVVTVQGEIERVLERIMQEPVSIDGAGRTDSGVHARRQVASFATCSPMPLGRL
IYSANSLLPSTIRINAMRQAPESFHARFSATSREYRYFLLEHPSAIDSRFAGCSHGKPDVGAMNRLALMLIGTHDFAAFS
KETPDQYGTLCTVTAARWYRSGRFHVFRIEANRFLRSMVRFLVAGMIEVGMGRLEEGAFARMLESGHRPPKLKPADAAGL
FLWKVRY
>Mature_246_residues
ARSKRTIKMQIEYDGTGYSGWQRQPGDVVTVQGEIERVLERIMQEPVSIDGAGRTDSGVHARRQVASFATCSPMPLGRLI
YSANSLLPSTIRINAMRQAPESFHARFSATSREYRYFLLEHPSAIDSRFAGCSHGKPDVGAMNRLALMLIGTHDFAAFSK
ETPDQYGTLCTVTAARWYRSGRFHVFRIEANRFLRSMVRFLVAGMIEVGMGRLEEGAFARMLESGHRPPKLKPADAAGLF
LWKVRY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=35.9183673469388, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLTE (Q8KDR7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661871.1
- ProteinModelPortal:   Q8KDR7
- SMR:   Q8KDR7
- GeneID:   1007052
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0978
- NMPDR:   fig|194439.1.peg.965
- TIGR:   CT0978
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- BioCyc:   CTEP194439:CT_0978-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27738; Mature: 27607

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: ACT_SITE 57-57

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKRTIKMQIEYDGTGYSGWQRQPGDVVTVQGEIERVLERIMQEPVSIDGAGRTDSGV
CCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCH
HARRQVASFATCSPMPLGRLIYSANSLLPSTIRINAMRQAPESFHARFSATSREYRYFLL
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEHHHHHCHHHHHHHHCCCCCCEEEEEE
EHPSAIDSRFAGCSHGKPDVGAMNRLALMLIGTHDFAAFSKETPDQYGTLCTVTAARWYR
ECCCHHHHHHCCCCCCCCCCCHHHHEEEEEEECCHHHHHCCCCCHHHCCEEEEEHHHHHC
SGRFHVFRIEANRFLRSMVRFLVAGMIEVGMGRLEEGAFARMLESGHRPPKLKPADAAGL
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEE
FLWKVRY
EEEEECC
>Mature Secondary Structure 
ARSKRTIKMQIEYDGTGYSGWQRQPGDVVTVQGEIERVLERIMQEPVSIDGAGRTDSGV
CCCCCEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCH
HARRQVASFATCSPMPLGRLIYSANSLLPSTIRINAMRQAPESFHARFSATSREYRYFLL
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEHHHHHCHHHHHHHHCCCCCCEEEEEE
EHPSAIDSRFAGCSHGKPDVGAMNRLALMLIGTHDFAAFSKETPDQYGTLCTVTAARWYR
ECCCHHHHHHCCCCCCCCCCCHHHHEEEEEEECCHHHHHCCCCCHHHCCEEEEEHHHHHC
SGRFHVFRIEANRFLRSMVRFLVAGMIEVGMGRLEEGAFARMLESGHRPPKLKPADAAGL
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEE
FLWKVRY
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901