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The map label for this gene is prfC [H]

Identifier: 21673647

GI number: 21673647

Start: 779123

End: 780718

Strand: Reverse

Name: prfC [H]

Synonym: CT0818

Alternate gene names: 21673647

Gene position: 780718-779123 (Counterclockwise)

Preceding gene: 21673648

Following gene: 21673646

Centisome position: 36.23

GC content: 58.71

Gene sequence:

>1596_bases
ATGGAGTTAGCAAAGGAGATCGCGCGGCGGCGGACGTTCGCTATTATCAGCCATCCGGATGCGGGTAAAACCACGCTCAC
CGAAAAACTACTGCTGTTCGGCGGTGCAATCCATACGGCGGGCGCGGTGAAGAGCAACAAGATTCGCAAGTCAGCGACCA
GCGACTTCATGGAGATCGAGAAGCAGCGCGGCATTTCGGTGGCGACCTCGGTGATGGGTTTCGAGTACAAGGGCAAGCGC
ATCAACATTCTCGACACGCCCGGCCACAAGGACTTCGCCGAAGACACCTATCGCACGCTGACCGCCGTGGACAGCGTCAT
CCTCGTGGTCGATAGCATGAAGGGCGTCGAGGAGCAGACCGAGCGGCTGATGGAGGTTTGCCGGATGCGCCACACGCCGG
TGATCATCTTCATTAACAAGCTCGACCGCGAAGGGCGCAACCCCTTTGAGCTGCTCGACGAGCTGGAAGAGAAGCTCGAT
ATCCAGACCTGCCCGATGACCTGGCCGATCAGCCAGGGCCAGACCTTCAAGGGCGTCTATAACCTGTTCGACAAATCGCT
GAACCTGTTCGAAGCCAACACCTCGCAGATCGGCCAGAAGCTGACCGACATCGAGGGCATCGACGACCCGAAACTCGCCG
ACTGGGTCGGCACGGCCAATGCCGCGAAGCTGCGCGAGGACGTCGAACTGATCGAGGGTGTGTATGAGCCGTACGAGCTT
GAGATGTACCGTGAAGGATTGCAGGCGCCGGTCTTTTTCGGTAGCGCGGTCAACAATTTTGGCGTCCGGGAACTGCTCGA
CACCTTCATCGACATCGCCCCGTGCCCGCATGAGCGCGAGGCGTCGGAGCGCATAGTGTACGCTTCTGAGGCGGCGCTCT
CCGGCTTCGTCTTCAAGATTCACGCCAACCTCGACCCGAACCACTGCGACCGCACGGCCTTCTTCAAAATCTGCTCCGGA
CGCTTCGAGCGCAACAAGTTCTACCAGCACACGCGCCTCGGTAAAAAGGTGCGCTTCTCCAACCCGACGCAGTTCATGGC
GCAGGAGAAGAACGTGATCGACGAAGCCTGGCCCGGCGACGTGATCGGCCTGTATGACAACGGTTCGCTGAAAATCGGCG
ACACGCTCACCGAAGGCGAAACGCTGCACTTTCGCGGCATTCCGAGCTTCTCGCCGGAGATCTTCAAGGTGCTCGAAAAC
CGCGACCCGCTCAAGACCAAGCAGCTCGAAAAGGGCATCCGCCAGCTCACCGATGAGGGCGTGGCGCAGCTCTTCGTGCA
GTACGGCACCCGTAAGATCGTCGGTACAGTGGGCGAACTTCAGTTCGATGTCATCCAGTTCCGCCTCGAACACGAGTACG
GCGCGCAGTGCTCTTTCACGCCCCTGCGCTTCCACAAAGCCTTCTGGATCACCAGCGACAACCAGAAGCAGCTCGACGAG
TTCATGCGCCGCCGCGCAAACGTCATCGCCTTCGACAAGGAGGATCACCCGGTCTTTTTCGCCGAAACCGAATGGATGCT
CAAAATCGCCAAAGAGGATTTTCCGGAGATTGAGTTTCACTCCACCTCGGAGTTCAAAACTAAAAATCAGGATTGA

Upstream 100 bases:

>100_bases
CCATCATCCTTCCCCATGCCCGATATTTCCGGTTTTTTGATATTTTCCGCACTCATCGAACAGAACCGGACTTGACACAT
CAGACAGAGAGACCAGAAGC

Downstream 100 bases:

>100_bases
TCATCGATTTTCACGAATGTTAATAAACCTTTCCGGTCATACATTAGTTACTGTAAAAACGTTGACCAGGAAACGATCGG
GAAATACGAACATGAAGCGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MELAKEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVKSNKIRKSATSDFMEIEKQRGISVATSVMGFEYKGKR
INILDTPGHKDFAEDTYRTLTAVDSVILVVDSMKGVEEQTERLMEVCRMRHTPVIIFINKLDREGRNPFELLDELEEKLD
IQTCPMTWPISQGQTFKGVYNLFDKSLNLFEANTSQIGQKLTDIEGIDDPKLADWVGTANAAKLREDVELIEGVYEPYEL
EMYREGLQAPVFFGSAVNNFGVRELLDTFIDIAPCPHEREASERIVYASEAALSGFVFKIHANLDPNHCDRTAFFKICSG
RFERNKFYQHTRLGKKVRFSNPTQFMAQEKNVIDEAWPGDVIGLYDNGSLKIGDTLTEGETLHFRGIPSFSPEIFKVLEN
RDPLKTKQLEKGIRQLTDEGVAQLFVQYGTRKIVGTVGELQFDVIQFRLEHEYGAQCSFTPLRFHKAFWITSDNQKQLDE
FMRRRANVIAFDKEDHPVFFAETEWMLKIAKEDFPEIEFHSTSEFKTKNQD

Sequences:

>Translated_531_residues
MELAKEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVKSNKIRKSATSDFMEIEKQRGISVATSVMGFEYKGKR
INILDTPGHKDFAEDTYRTLTAVDSVILVVDSMKGVEEQTERLMEVCRMRHTPVIIFINKLDREGRNPFELLDELEEKLD
IQTCPMTWPISQGQTFKGVYNLFDKSLNLFEANTSQIGQKLTDIEGIDDPKLADWVGTANAAKLREDVELIEGVYEPYEL
EMYREGLQAPVFFGSAVNNFGVRELLDTFIDIAPCPHEREASERIVYASEAALSGFVFKIHANLDPNHCDRTAFFKICSG
RFERNKFYQHTRLGKKVRFSNPTQFMAQEKNVIDEAWPGDVIGLYDNGSLKIGDTLTEGETLHFRGIPSFSPEIFKVLEN
RDPLKTKQLEKGIRQLTDEGVAQLFVQYGTRKIVGTVGELQFDVIQFRLEHEYGAQCSFTPLRFHKAFWITSDNQKQLDE
FMRRRANVIAFDKEDHPVFFAETEWMLKIAKEDFPEIEFHSTSEFKTKNQD
>Mature_531_residues
MELAKEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVKSNKIRKSATSDFMEIEKQRGISVATSVMGFEYKGKR
INILDTPGHKDFAEDTYRTLTAVDSVILVVDSMKGVEEQTERLMEVCRMRHTPVIIFINKLDREGRNPFELLDELEEKLD
IQTCPMTWPISQGQTFKGVYNLFDKSLNLFEANTSQIGQKLTDIEGIDDPKLADWVGTANAAKLREDVELIEGVYEPYEL
EMYREGLQAPVFFGSAVNNFGVRELLDTFIDIAPCPHEREASERIVYASEAALSGFVFKIHANLDPNHCDRTAFFKICSG
RFERNKFYQHTRLGKKVRFSNPTQFMAQEKNVIDEAWPGDVIGLYDNGSLKIGDTLTEGETLHFRGIPSFSPEIFKVLEN
RDPLKTKQLEKGIRQLTDEGVAQLFVQYGTRKIVGTVGELQFDVIQFRLEHEYGAQCSFTPLRFHKAFWITSDNQKQLDE
FMRRRANVIAFDKEDHPVFFAETEWMLKIAKEDFPEIEFHSTSEFKTKNQD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=29.0123456790123, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI19923640, Length=565, Percent_Identity=25.4867256637168, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306287, Length=537, Percent_Identity=25.1396648044693, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI25306283, Length=295, Percent_Identity=31.5254237288136, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=34.3949044585987, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=31.2925170068027, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=27.6315789473684, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310132016, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI310110807, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI310123363, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=46.8330134357006, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1789738, Length=503, Percent_Identity=29.0258449304175, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=208, Percent_Identity=31.25, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=517, Percent_Identity=24.9516441005803, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=169, Percent_Identity=36.094674556213, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.8518518518519, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=30.1282051282051, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=25.4201680672269, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6322359, Length=517, Percent_Identity=24.7582205029014, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=28.6624203821656, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=28.6624203821656, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=31.1594202898551, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24582462, Length=497, Percent_Identity=27.5653923541247, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=25.875486381323, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI78706572, Length=154, Percent_Identity=35.0649350649351, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60721; Mature: 60721

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAKEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVKSNKIRKSATSDFMEIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHH
KQRGISVATSVMGFEYKGKRINILDTPGHKDFAEDTYRTLTAVDSVILVVDSMKGVEEQT
HHCCCHHHHHHHCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERLMEVCRMRHTPVIIFINKLDREGRNPFELLDELEEKLDIQTCPMTWPISQGQTFKGVY
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHH
NLFDKSLNLFEANTSQIGQKLTDIEGIDDPKLADWVGTANAAKLREDVELIEGVYEPYEL
HHHHHCCHHEECCHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
EMYREGLQAPVFFGSAVNNFGVRELLDTFIDIAPCPHEREASERIVYASEAALSGFVFKI
HHHHCCCCCCEEECCHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCEEEEE
HANLDPNHCDRTAFFKICSGRFERNKFYQHTRLGKKVRFSNPTQFMAQEKNVIDEAWPGD
ECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCC
VIGLYDNGSLKIGDTLTEGETLHFRGIPSFSPEIFKVLENRDPLKTKQLEKGIRQLTDEG
EEEEECCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VAQLFVQYGTRKIVGTVGELQFDVIQFRLEHEYGAQCSFTPLRFHKAFWITSDNQKQLDE
HHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCHHHHHH
FMRRRANVIAFDKEDHPVFFAETEWMLKIAKEDFPEIEFHSTSEFKTKNQD
HHHHCCCEEEECCCCCCEEEECHHHHHHHHHCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MELAKEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVKSNKIRKSATSDFMEIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHH
KQRGISVATSVMGFEYKGKRINILDTPGHKDFAEDTYRTLTAVDSVILVVDSMKGVEEQT
HHCCCHHHHHHHCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ERLMEVCRMRHTPVIIFINKLDREGRNPFELLDELEEKLDIQTCPMTWPISQGQTFKGVY
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHH
NLFDKSLNLFEANTSQIGQKLTDIEGIDDPKLADWVGTANAAKLREDVELIEGVYEPYEL
HHHHHCCHHEECCHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
EMYREGLQAPVFFGSAVNNFGVRELLDTFIDIAPCPHEREASERIVYASEAALSGFVFKI
HHHHCCCCCCEEECCHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCEEEEE
HANLDPNHCDRTAFFKICSGRFERNKFYQHTRLGKKVRFSNPTQFMAQEKNVIDEAWPGD
ECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCC
VIGLYDNGSLKIGDTLTEGETLHFRGIPSFSPEIFKVLENRDPLKTKQLEKGIRQLTDEG
EEEEECCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VAQLFVQYGTRKIVGTVGELQFDVIQFRLEHEYGAQCSFTPLRFHKAFWITSDNQKQLDE
HHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCHHHHHH
FMRRRANVIAFDKEDHPVFFAETEWMLKIAKEDFPEIEFHSTSEFKTKNQD
HHHHCCCEEEECCCCCCEEEECHHHHHHHHHCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA