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The map label for this gene is ksgA

Identifier: 21673632

GI number: 21673632

Start: 767218

End: 768045

Strand: Reverse

Name: ksgA

Synonym: CT0803

Alternate gene names: 21673632

Gene position: 768045-767218 (Counterclockwise)

Preceding gene: 21673637

Following gene: 21673631

Centisome position: 35.64

GC content: 56.16

Gene sequence:

>828_bases
ATGACAAAAGTTGAATATAAGCATACGCATATAGCGGCAAAAAAAAAATTAGGCCAAAACTTCCTGCTCGACAGGAACAT
TCCGCGCAAGATCGTCCGGGAATCGGGCATCAAGGAGGGCGACCGGGTGGTTGAGATTGGTCCCGGCTTTGGCGCTCTGA
CGACGGCGATTCTCGAAGTGATGCCGTCGTTCACCGCCATCGAGAAAGACCGGGAGCTGGCGAAGTTCAACCGCGAGGAG
CATCCGCAGATCGAGCTGATCGAAGACGACTTCCTCAAAGTACCACTTGAACCGCTGGCTGCAGGCGGCAAACTCTCGGT
GCTCGGCAACATTCCCTACTCGATCACCAGCCCGATCCTCTTCCGGCTGCTCGATAACCGGCACCTCATCGCCTCGGCCA
CGCTGATGATACAGCACGAAGTCGCCCAGCGCATCGCCGCCGTACCCGGCACGAAAGAGTACGGCATCCTCGCCGTGCAG
ATGCAGGCCTTCTGTGACGTGAAATATCTGTTCAAGGTAGGCCGCGCCGTCTTCAAACCCCGCCCGGATGTGGACAGCGC
CGTCATCAAAATGGTGCCGAAGGCTGTCGATCCGGTCAAAGACAGCGAAGGATTCCGAACCTTCGTCCGCCGTGTTTTCC
ACCAGCGCAGGAAAACACTTCTGAATAACCTGAAGGAGTATTACGACACTTCAGGAGTACCGGAACCCACGCTCAAACTG
CGTGCGGAGTCGCTCTCCGTTCCGGCGCTGATCACCCTGTTCACTCAGCTCAAACTGATTGCGCGAGGCGACGCCTCCGG
ACAACTTTTATTAAAGAGAAGAAGATGA

Upstream 100 bases:

>100_bases
TGACAGAATACCTTAAATTTGAAAAAGCTATGCAGGACCTGAGTGCCTGCCGGGTTAACCCCGCCATTGATATTGAGTTG
CGCCCACCAAGCCCAGTCAG

Downstream 100 bases:

>100_bases
GAAGACAGCAATCGTTCCAGTGATGTAATCTCTTATTTTCATTAGAATTTTATTTTCAGATCAACATCCAGCCATTCGAG
GACATGCGGGACTCACAGCA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTKVEYKHTHIAAKKKLGQNFLLDRNIPRKIVRESGIKEGDRVVEIGPGFGALTTAILEVMPSFTAIEKDRELAKFNREE
HPQIELIEDDFLKVPLEPLAAGGKLSVLGNIPYSITSPILFRLLDNRHLIASATLMIQHEVAQRIAAVPGTKEYGILAVQ
MQAFCDVKYLFKVGRAVFKPRPDVDSAVIKMVPKAVDPVKDSEGFRTFVRRVFHQRRKTLLNNLKEYYDTSGVPEPTLKL
RAESLSVPALITLFTQLKLIARGDASGQLLLKRRR

Sequences:

>Translated_275_residues
MTKVEYKHTHIAAKKKLGQNFLLDRNIPRKIVRESGIKEGDRVVEIGPGFGALTTAILEVMPSFTAIEKDRELAKFNREE
HPQIELIEDDFLKVPLEPLAAGGKLSVLGNIPYSITSPILFRLLDNRHLIASATLMIQHEVAQRIAAVPGTKEYGILAVQ
MQAFCDVKYLFKVGRAVFKPRPDVDSAVIKMVPKAVDPVKDSEGFRTFVRRVFHQRRKTLLNNLKEYYDTSGVPEPTLKL
RAESLSVPALITLFTQLKLIARGDASGQLLLKRRR
>Mature_274_residues
TKVEYKHTHIAAKKKLGQNFLLDRNIPRKIVRESGIKEGDRVVEIGPGFGALTTAILEVMPSFTAIEKDRELAKFNREEH
PQIELIEDDFLKVPLEPLAAGGKLSVLGNIPYSITSPILFRLLDNRHLIASATLMIQHEVAQRIAAVPGTKEYGILAVQM
QAFCDVKYLFKVGRAVFKPRPDVDSAVIKMVPKAVDPVKDSEGFRTFVRRVFHQRRKTLLNNLKEYYDTSGVPEPTLKLR
AESLSVPALITLFTQLKLIARGDASGQLLLKRRR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=210, Percent_Identity=32.3809523809524, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI156415992, Length=257, Percent_Identity=29.1828793774319, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786236, Length=253, Percent_Identity=36.7588932806324, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=34.2857142857143, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=245, Percent_Identity=31.4285714285714, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=210, Percent_Identity=32.3809523809524, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21358017, Length=221, Percent_Identity=32.579185520362, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357273, Length=202, Percent_Identity=31.1881188118812, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CHLTE (Q8KE87)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661697.1
- ProteinModelPortal:   Q8KE87
- SMR:   Q8KE87
- GeneID:   1006441
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0803
- NMPDR:   fig|194439.1.peg.791
- TIGR:   CT0803
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   CTEP194439:CT_0803-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30936; Mature: 30805

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 20-20 BINDING 22-22 BINDING 47-47 BINDING 68-68 BINDING 90-90 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVEYKHTHIAAKKKLGQNFLLDRNIPRKIVRESGIKEGDRVVEIGPGFGALTTAILEV
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
MPSFTAIEKDRELAKFNREEHPQIELIEDDFLKVPLEPLAAGGKLSVLGNIPYSITSPIL
HCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCHHHCCCCEEEEEECCCHHHHHHHH
FRLLDNRHLIASATLMIQHEVAQRIAAVPGTKEYGILAVQMQAFCDVKYLFKVGRAVFKP
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
RPDVDSAVIKMVPKAVDPVKDSEGFRTFVRRVFHQRRKTLLNNLKEYYDTSGVPEPTLKL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RAESLSVPALITLFTQLKLIARGDASGQLLLKRRR
HHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCC
>Mature Secondary Structure 
TKVEYKHTHIAAKKKLGQNFLLDRNIPRKIVRESGIKEGDRVVEIGPGFGALTTAILEV
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
MPSFTAIEKDRELAKFNREEHPQIELIEDDFLKVPLEPLAAGGKLSVLGNIPYSITSPIL
HCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCHHHCCCCEEEEEECCCHHHHHHHH
FRLLDNRHLIASATLMIQHEVAQRIAAVPGTKEYGILAVQMQAFCDVKYLFKVGRAVFKP
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
RPDVDSAVIKMVPKAVDPVKDSEGFRTFVRRVFHQRRKTLLNNLKEYYDTSGVPEPTLKL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RAESLSVPALITLFTQLKLIARGDASGQLLLKRRR
HHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901