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The map label for this gene is ndhI [H]

Identifier: 21673601

GI number: 21673601

Start: 743350

End: 744000

Strand: Direct

Name: ndhI [H]

Synonym: CT0771

Alternate gene names: 21673601

Gene position: 743350-744000 (Clockwise)

Preceding gene: 21673600

Following gene: 21673602

Centisome position: 34.5

GC content: 58.53

Gene sequence:

>651_bases
ATGAGTGAGTACTTCAGCAATATAAAGACCAGTGTGACCACCATCGCGACCGGCATGGGCATCACCCTCAAGCACTTCTT
CAATGCGGTCAAGCGCAAGGGAGATGCGGGTATCGACGATGCCGACTATTTTCGCCAGGTTGACGGTCTGTGCACCTTGC
AGTATCCGAAGGAGGCGATTCCGACGCCGCCGCATGGCCGTTACCGGCTGTATTGCAACATCAACGACTGCATCGGCTGC
AAGCAGTGCGAACGCGCCTGTCCGGTGGAGTGCATCACCATCGAAACCATCAAGACGACCAGCGATGATCTGGAAGCTTG
CGGCAAAACCTCCGGCGGACAGCAGAAGCGCATGTGGGTGCCGGTTTTCGACATCGATTTGGCCAAGTGCATGACCTGCG
GCATCTGCCAGAGTGTTTGCCCGACCGACTGCCTCTATCATACACCCGTCGCCGACTTCTCGGAGTTTGACGTCAGCAAT
ATGATGTACCACTTCGGTAACCTCTCGAAGATCGAGGCCGAAGCCAAAAGGAAAAAGCTTGCCGAACAGCAGGCCCAGGC
GGCAAAGGAGAAAGCCGCGGCTGGCGGCGCTCCGGCACCGAAACCGGCTCCAAAGGCCGCTCCGCAGCCGAACCCGGGCG
ACGCCCGGTAA

Upstream 100 bases:

>100_bases
CCTGGTTTCATTTGTGCTGACAGCCATCTGGGTGATCAACCATTAATCGTGAGGCTGGCTCAAGAGAGAATACAAAGGCA
AGCAAGACGAACAACAAACT

Downstream 100 bases:

>100_bases
GCATCCGGAAGGAATTGATTACCAACAAGTGCAGATAACCATGAATCAACTGACCATCGCCATCATCTTCTACATCTTCG
CGGCCGTTACGGTACTGTCG

Product: NADH dehydrogenase I, 23 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 2; NDH-1 subunit I 2 [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSEYFSNIKTSVTTIATGMGITLKHFFNAVKRKGDAGIDDADYFRQVDGLCTLQYPKEAIPTPPHGRYRLYCNINDCIGC
KQCERACPVECITIETIKTTSDDLEACGKTSGGQQKRMWVPVFDIDLAKCMTCGICQSVCPTDCLYHTPVADFSEFDVSN
MMYHFGNLSKIEAEAKRKKLAEQQAQAAKEKAAAGGAPAPKPAPKAAPQPNPGDAR

Sequences:

>Translated_216_residues
MSEYFSNIKTSVTTIATGMGITLKHFFNAVKRKGDAGIDDADYFRQVDGLCTLQYPKEAIPTPPHGRYRLYCNINDCIGC
KQCERACPVECITIETIKTTSDDLEACGKTSGGQQKRMWVPVFDIDLAKCMTCGICQSVCPTDCLYHTPVADFSEFDVSN
MMYHFGNLSKIEAEAKRKKLAEQQAQAAKEKAAAGGAPAPKPAPKAAPQPNPGDAR
>Mature_215_residues
SEYFSNIKTSVTTIATGMGITLKHFFNAVKRKGDAGIDDADYFRQVDGLCTLQYPKEAIPTPPHGRYRLYCNINDCIGCK
QCERACPVECITIETIKTTSDDLEACGKTSGGQQKRMWVPVFDIDLAKCMTCGICQSVCPTDCLYHTPVADFSEFDVSNM
MYHFGNLSKIEAEAKRKKLAEQQAQAAKEKAAAGGAPAPKPAPKAAPQPNPGDAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=171, Percent_Identity=28.6549707602339, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788617, Length=122, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23569; Mature: 23438

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
6.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYFSNIKTSVTTIATGMGITLKHFFNAVKRKGDAGIDDADYFRQVDGLCTLQYPKEAI
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEECCCHHHC
PTPPHGRYRLYCNINDCIGCKQCERACPVECITIETIKTTSDDLEACGKTSGGQQKRMWV
CCCCCCCEEEEEECCHHCCHHHHHHCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCEEEE
PVFDIDLAKCMTCGICQSVCPTDCLYHTPVADFSEFDVSNMMYHFGNLSKIEAEAKRKKL
EEHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AEQQAQAAKEKAAAGGAPAPKPAPKAAPQPNPGDAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEYFSNIKTSVTTIATGMGITLKHFFNAVKRKGDAGIDDADYFRQVDGLCTLQYPKEAI
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEECCCHHHC
PTPPHGRYRLYCNINDCIGCKQCERACPVECITIETIKTTSDDLEACGKTSGGQQKRMWV
CCCCCCCEEEEEECCHHCCHHHHHHCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCEEEE
PVFDIDLAKCMTCGICQSVCPTDCLYHTPVADFSEFDVSNMMYHFGNLSKIEAEAKRKKL
EEHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AEQQAQAAKEKAAAGGAPAPKPAPKAAPQPNPGDAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]