Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 21673533

GI number: 21673533

Start: 687859

End: 688353

Strand: Reverse

Name: mdeA [H]

Synonym: CT0703

Alternate gene names: 21673533

Gene position: 688353-687859 (Counterclockwise)

Preceding gene: 21673535

Following gene: 21673532

Centisome position: 31.94

GC content: 64.04

Gene sequence:

>495_bases
GTGCGCACCGTGCCACTCAGGAACCTCGGCGCGGCAATCTCACCCGACAACTCGTGGATTTTCATCCAGGGCGTCGAGAC
GCTGCCGGTGCGCATGATCCGCCACTCCGAAAACGCGCTGAAGGTGGCCGGGCATCTGAAAAATCACCCGAAGGTCGCCT
GGGTGCGCTACCCCGGTCTGCCGGATGACCCGTCGTACGCACTCGCCTCGCGCGATCTGAAGCGCGGTTTCGGCGGCATG
GTGGTATTCGGGGTTAAGGGTGGCTACGACGCGGCGGTGAAGATCATCGACACCATCGACCTCTTCTCGCACCTGGCCAA
CGTCGGCGACGCCAAGAGCCTGATCCTGCACCCGGCAAGCACCTCGCACAGCCAAATGACAGAGGAGCAGCGAGTCGCAA
GCGGTTTGTCGTCCGACCTCATCCGCCTCTCGATCGGCCTGGAGCACCCCGACGACCTCATCGCCGCGCTCGACGACGTA
CTGGCGGGAGTATGA

Upstream 100 bases:

>100_bases
ACGCCATCCGCTCTTCACCGAGCCGGATGCCAACTACCACGGCCTGCGCTGGGCCATCGACCTGCCGGAACCGCTCGCGC
CAATCGCCTTCGCGCTCCGC

Downstream 100 bases:

>100_bases
ATTTGTTGTGAATGATAGACAGAAGACCGGCTGGAGATGCCGGTTTTCTTGACCGTAACACTTTTCAACCAAGCGATGCA
GGCCGCTCTTTGCCTTGCTC

Product: trans-sulfuration enzyme family protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRTVPLRNLGAAISPDNSWIFIQGVETLPVRMIRHSENALKVAGHLKNHPKVAWVRYPGLPDDPSYALASRDLKRGFGGM
VVFGVKGGYDAAVKIIDTIDLFSHLANVGDAKSLILHPASTSHSQMTEEQRVASGLSSDLIRLSIGLEHPDDLIAALDDV
LAGV

Sequences:

>Translated_164_residues
MRTVPLRNLGAAISPDNSWIFIQGVETLPVRMIRHSENALKVAGHLKNHPKVAWVRYPGLPDDPSYALASRDLKRGFGGM
VVFGVKGGYDAAVKIIDTIDLFSHLANVGDAKSLILHPASTSHSQMTEEQRVASGLSSDLIRLSIGLEHPDDLIAALDDV
LAGV
>Mature_164_residues
MRTVPLRNLGAAISPDNSWIFIQGVETLPVRMIRHSENALKVAGHLKNHPKVAWVRYPGLPDDPSYALASRDLKRGFGGM
VVFGVKGGYDAAVKIIDTIDLFSHLANVGDAKSLILHPASTSHSQMTEEQRVASGLSSDLIRLSIGLEHPDDLIAALDDV
LAGV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=139, Percent_Identity=37.410071942446, Blast_Score=90, Evalue=8e-19,
Organism=Homo sapiens, GI25453487, Length=139, Percent_Identity=37.410071942446, Blast_Score=90, Evalue=8e-19,
Organism=Homo sapiens, GI299473759, Length=139, Percent_Identity=37.410071942446, Blast_Score=90, Evalue=9e-19,
Organism=Escherichia coli, GI1790375, Length=152, Percent_Identity=32.2368421052632, Blast_Score=94, Evalue=3e-21,
Organism=Escherichia coli, GI1789383, Length=152, Percent_Identity=35.5263157894737, Blast_Score=78, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71980708, Length=152, Percent_Identity=37.5, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538055, Length=140, Percent_Identity=32.1428571428571, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560056, Length=142, Percent_Identity=31.6901408450704, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323333, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319307, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321254, Length=168, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922554, Length=157, Percent_Identity=33.1210191082803, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 17670; Mature: 17670

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVPLRNLGAAISPDNSWIFIQGVETLPVRMIRHSENALKVAGHLKNHPKVAWVRYPGL
CCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCC
PDDPSYALASRDLKRGFGGMVVFGVKGGYDAAVKIIDTIDLFSHLANVGDAKSLILHPAS
CCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TSHSQMTEEQRVASGLSSDLIRLSIGLEHPDDLIAALDDVLAGV
CCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTVPLRNLGAAISPDNSWIFIQGVETLPVRMIRHSENALKVAGHLKNHPKVAWVRYPGL
CCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCC
PDDPSYALASRDLKRGFGGMVVFGVKGGYDAAVKIIDTIDLFSHLANVGDAKSLILHPAS
CCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TSHSQMTEEQRVASGLSSDLIRLSIGLEHPDDLIAALDDVLAGV
CCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]