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The map label for this gene is ybjS [C]

Identifier: 21673411

GI number: 21673411

Start: 582995

End: 584002

Strand: Direct

Name: ybjS [C]

Synonym: CT0576

Alternate gene names: 21673411

Gene position: 582995-584002 (Clockwise)

Preceding gene: 21673410

Following gene: 21673412

Centisome position: 27.05

GC content: 63.29

Gene sequence:

>1008_bases
ATGGCCAAAACCGTTCTTGTTACCGGCGCGTCCGGGTTTATCGGGTCGCATCTGGTGGGGCGGTGCTTGGCGGATGGCTG
TCGCGTGAAGGCGCTGGTGCGGAAGGGGAATGCATGCATCGATTCGCTGCGGGCTTCCGGTGTGGAGGTCATCGAGGGGG
ATGTGCGTGATGCCACCGCGGTCGATGCCGCCGTGCGTGAGAGTGACCTCGTGCTGCATGCTGCTGCGCTGACCTCCGAC
TGGGGGAAGATGCAGGAGTTCATCGACATCAACGTTGGCGGTACGCGCAACGTGTGCGAAGCGTCGCTTCGGCATGGTGT
CGGACGGCTGGTGCATGTCAGTTCGTTCGAGTGTTTTGATCACCACTTGCTTGGCCGCATCGACGAGCAGACCCCCTACA
AGGCGCGGAAGCAGTCCTATCCCGATACGAAAATCGGCGGCACAAACGAGGTGTGGGCGGCCATAAAGCGGGGGCTTTCA
GCAAGCATTCTCTACCCGGTGTGGGTCTATGGCCCCGGTGACCGGACGCTGTTTCCGCTGCTGGCCGACAGCATCCTCCG
GCGGCAACTCTTTTTCTGGGCGCGCAACGCGCCGATGAGCATGATTTATATCGACAACCTCGTCGATCTGACGATGCTGG
CCGCCTCCCGTCCGGAAGCGGTGGGCGAGGCGTTCATGGCCTGCGACGGCGAGGCGATCACTTTCGAGGAGGTGTGCCGG
CGCGTTGCCGTGGCGATTGGCTCGCCGGTGCCATCGCTTCACCTGCCGTTTGGCATGGTTCGGTCCGTTGCCGGGGTGAT
GGAGTTTGTCTGGCGGATCGCCGGAAGTAAGAAGCGTCCGCTCCTCACCCGGCAGGCGGTTGATGTGCTCGCCTCGCGGG
CGCTGGCCGACGTGTCGAAAGCCCGCACGATGCTTGGCTGGCAAAGCCACGTGCCGCAGGAGGAGGGAATCCGTCGCACG
CTGGAGTGGCTGGTGACGGTCGATCCGGCGCGGTGGAAGGTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGACTCTGTGCGAAAGCGGTATTCGCAAGGCAGTGGAGAACTGCCCACTGTGGGAAGGGGGACAGCAGAGTGAAAAC
CGTATCGACAGTTTGTAATT

Downstream 100 bases:

>100_bases
TGTTTCGCCTCCGCCAGAAAAAAAGTTGTTTCCGGGGCTTCGCCCGTAAAGCGTTTTGGTGTCCGCATCGCGCGGGTTTA
CCCTCGTTAAGTCACCCGTC

Product: NAD(P)-dependent cholesterol dehydrogenase, putative

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAKTVLVTGASGFIGSHLVGRCLADGCRVKALVRKGNACIDSLRASGVEVIEGDVRDATAVDAAVRESDLVLHAAALTSD
WGKMQEFIDINVGGTRNVCEASLRHGVGRLVHVSSFECFDHHLLGRIDEQTPYKARKQSYPDTKIGGTNEVWAAIKRGLS
ASILYPVWVYGPGDRTLFPLLADSILRRQLFFWARNAPMSMIYIDNLVDLTMLAASRPEAVGEAFMACDGEAITFEEVCR
RVAVAIGSPVPSLHLPFGMVRSVAGVMEFVWRIAGSKKRPLLTRQAVDVLASRALADVSKARTMLGWQSHVPQEEGIRRT
LEWLVTVDPARWKVK

Sequences:

>Translated_335_residues
MAKTVLVTGASGFIGSHLVGRCLADGCRVKALVRKGNACIDSLRASGVEVIEGDVRDATAVDAAVRESDLVLHAAALTSD
WGKMQEFIDINVGGTRNVCEASLRHGVGRLVHVSSFECFDHHLLGRIDEQTPYKARKQSYPDTKIGGTNEVWAAIKRGLS
ASILYPVWVYGPGDRTLFPLLADSILRRQLFFWARNAPMSMIYIDNLVDLTMLAASRPEAVGEAFMACDGEAITFEEVCR
RVAVAIGSPVPSLHLPFGMVRSVAGVMEFVWRIAGSKKRPLLTRQAVDVLASRALADVSKARTMLGWQSHVPQEEGIRRT
LEWLVTVDPARWKVK
>Mature_334_residues
AKTVLVTGASGFIGSHLVGRCLADGCRVKALVRKGNACIDSLRASGVEVIEGDVRDATAVDAAVRESDLVLHAAALTSDW
GKMQEFIDINVGGTRNVCEASLRHGVGRLVHVSSFECFDHHLLGRIDEQTPYKARKQSYPDTKIGGTNEVWAAIKRGLSA
SILYPVWVYGPGDRTLFPLLADSILRRQLFFWARNAPMSMIYIDNLVDLTMLAASRPEAVGEAFMACDGEAITFEEVCRR
VAVAIGSPVPSLHLPFGMVRSVAGVMEFVWRIAGSKKRPLLTRQAVDVLASRALADVSKARTMLGWQSHVPQEEGIRRTL
EWLVTVDPARWKVK

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=349, Percent_Identity=27.5071633237822, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI310132178, Length=330, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI310113012, Length=330, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI239745448, Length=330, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI193211614, Length=337, Percent_Identity=24.3323442136499, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI8393516, Length=337, Percent_Identity=24.3323442136499, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI19923621, Length=354, Percent_Identity=25.9887005649718, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI87081792, Length=336, Percent_Identity=27.6785714285714, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321437, Length=352, Percent_Identity=26.9886363636364, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 36698; Mature: 36567

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVLVTGASGFIGSHLVGRCLADGCRVKALVRKGNACIDSLRASGVEVIEGDVRDATA
CCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCHHHH
VDAAVRESDLVLHAAALTSDWGKMQEFIDINVGGTRNVCEASLRHGVGRLVHVSSFECFD
HHHHHHHHHHEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCHHEEEECCHHHHH
HHLLGRIDEQTPYKARKQSYPDTKIGGTNEVWAAIKRGLSASILYPVWVYGPGDRTLFPL
HHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHH
LADSILRRQLFFWARNAPMSMIYIDNLVDLTMLAASRPEAVGEAFMACDGEAITFEEVCR
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECHHHHHH
RVAVAIGSPVPSLHLPFGMVRSVAGVMEFVWRIAGSKKRPLLTRQAVDVLASRALADVSK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ARTMLGWQSHVPQEEGIRRTLEWLVTVDPARWKVK
HHHHHHHHHCCCHHHHHHHHHHHHEEECCCCEECC
>Mature Secondary Structure 
AKTVLVTGASGFIGSHLVGRCLADGCRVKALVRKGNACIDSLRASGVEVIEGDVRDATA
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCHHHH
VDAAVRESDLVLHAAALTSDWGKMQEFIDINVGGTRNVCEASLRHGVGRLVHVSSFECFD
HHHHHHHHHHEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCHHEEEECCHHHHH
HHLLGRIDEQTPYKARKQSYPDTKIGGTNEVWAAIKRGLSASILYPVWVYGPGDRTLFPL
HHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHH
LADSILRRQLFFWARNAPMSMIYIDNLVDLTMLAASRPEAVGEAFMACDGEAITFEEVCR
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECHHHHHH
RVAVAIGSPVPSLHLPFGMVRSVAGVMEFVWRIAGSKKRPLLTRQAVDVLASRALADVSK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ARTMLGWQSHVPQEEGIRRTLEWLVTVDPARWKVK
HHHHHHHHHCCCHHHHHHHHHHHHEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]