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The map label for this gene is nadE

Identifier: 21673395

GI number: 21673395

Start: 567116

End: 567949

Strand: Direct

Name: nadE

Synonym: CT0560

Alternate gene names: 21673395

Gene position: 567116-567949 (Clockwise)

Preceding gene: 21673394

Following gene: 21673404

Centisome position: 26.32

GC content: 58.87

Gene sequence:

>834_bases
ATGAAACCGCAAAACCTCCATTTCGATTACGGCTTGGTTGAAGCCATCCTGGTTCCTTTCATCCGCAACGAAATCAGGAA
GTTCGGCTTCGGCTCGGTAGTGCTTGGCCTGTCGGGTGGCATCGATTCGGCGGTGGTGTGCGAGCTCGCCGTCCGGGCAC
TTGGCGTGGAGAATGTGCTGGCGCTGATGATGCCCTACAAGACGAGCAGTCAGGAAAGTCTCGATCACGCCGAATTGATG
GTTGATCGGCTCGGCATACGATACGAGATCATGCCGGTGACAGAGGTGGTTGACGCCTTCTTCGCCACGCGTCCCGACGC
CAGCCGTCTGCGGCGGGGCAACGTGATGGCGCGGTCGCGAATGCTCTGTCTCTACGACGTCTCGGCGCGGGATGGCTGCC
TGGTGCTCGGCACGAGCAACAAGACCGAGCTGATGCTCGGCTATGGCACCATGTTTGGTGACATGGCCTCTGCGGTCAAC
CCTATTGGCGACCTTTACAAGACGCAGATTTTCGGCCTCGCAAGGCACCTCGGCATTCCCGCGCCGCTCATCGACAAACC
GCCATCCGCCGATCTCTGGGAAGGGCAAAGCGACGAAGCCGATCTTGGTTTTAGCTACGAGGAGGTTGACCAGCTTTTGT
ATATGATGCTCGAAGAGCGTATGGATCGTGATGCCATTCTCGCCGAAGGCATCGACTCAGCCTTCTACCAGCGAGTGCGC
AGCATGGTGGTGCGGAACCAGTACAAGCGTATGATGCCCGTCATTGCCAAACTCTCCAGCCGCACGCCAGGCATCGATTT
CCGGTACGCGCGTGACTGGCAGGAGGTGCGGTAG

Upstream 100 bases:

>100_bases
TCAGGAAAATCGGCTTTTCTGGTAGGCGCGATGGCGATGATCTCATCCCTGCTCCAGAACGGTCTTATCCGTTTTTTTAT
CTCTATCTGACTGAAAATCC

Downstream 100 bases:

>100_bases
GGGAGCGTTTGCATGGGCCGGTGATGCCTGTGGAGCTTTGGGAAACATTCCCTGATCTTTTTTCCCAAAAGTCCCGAAAA
TCCCACTTCTCAAAACGACC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKPQNLHFDYGLVEAILVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVLALMMPYKTSSQESLDHAELM
VDRLGIRYEIMPVTEVVDAFFATRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQIFGLARHLGIPAPLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVR
SMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEVR

Sequences:

>Translated_277_residues
MKPQNLHFDYGLVEAILVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVLALMMPYKTSSQESLDHAELM
VDRLGIRYEIMPVTEVVDAFFATRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQIFGLARHLGIPAPLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVR
SMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEVR
>Mature_277_residues
MKPQNLHFDYGLVEAILVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVLALMMPYKTSSQESLDHAELM
VDRLGIRYEIMPVTEVVDAFFATRPDASRLRRGNVMARSRMLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVN
PIGDLYKTQIFGLARHLGIPAPLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVR
SMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEVR

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_CHLTE (Q8KEX2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661460.1
- ProteinModelPortal:   Q8KEX2
- SMR:   Q8KEX2
- GeneID:   1006143
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0560
- NMPDR:   fig|194439.1.peg.554
- TIGR:   CT0560
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- BioCyc:   CTEP194439:CT_0560-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 31125; Mature: 31125

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQNLHFDYGLVEAILVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHH
ALMMPYKTSSQESLDHAELMVDRLGIRYEIMPVTEVVDAFFATRPDASRLRRGNVMARSR
HHHHCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCHHHHHHCCHHHHHC
MLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVNPIGDLYKTQIFGLARHLGIP
EEEEEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
APLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCC
>Mature Secondary Structure
MKPQNLHFDYGLVEAILVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHH
ALMMPYKTSSQESLDHAELMVDRLGIRYEIMPVTEVVDAFFATRPDASRLRRGNVMARSR
HHHHCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCHHHHHHCCHHHHHC
MLCLYDVSARDGCLVLGTSNKTELMLGYGTMFGDMASAVNPIGDLYKTQIFGLARHLGIP
EEEEEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
APLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SMVVRNQYKRMMPVIAKLSSRTPGIDFRYARDWQEVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901