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The map label for this gene is lptD [H]

Identifier: 21673358

GI number: 21673358

Start: 528421

End: 531312

Strand: Direct

Name: lptD [H]

Synonym: CT0523

Alternate gene names: 21673358

Gene position: 528421-531312 (Clockwise)

Preceding gene: 21673344

Following gene: 21673360

Centisome position: 24.52

GC content: 54.98

Gene sequence:

>2892_bases
ATGAGTTGTTCCGGAGAGGTATCGATTAAGGAGAAAGGTTTTATATATTCATGTTCTGATTTGCATGACGAACATCATCA
GCGCCCGCTAACCCAGCATGATCCGCCCGTGAAATTCACCAAGTCAATCAAACTTCTGACTCTGTTGCTCCTCGTCCAGT
CTTTCGAGGGGATTTTGCCGCTGCGTGCGGGTGAAAGTGCATCGAAACCGCAAAAAGCAAAAGATGGCGCGTTGCCCGAC
AGCTTGTTGGAAAAGCGTGAGGATCTTGACTCGACTGTTGTGTACACGGCACGGGATTCGCTGATCTACAACGTCAGCAA
GCGTACTGCCGACCTGTTCGGCAAGGCGAAAGTCAATTACAAGGACAGCCGCATCGAAGGCCCCAGAATCACCATTGAAC
AGGCGACCTCTACGGCGCGCGCCACGGCTTCACGCGACTCTCTTGGCCGTCCAGCCGAGCTTCCCGTCTATACCGGCAAG
GACGGATCGTTCAGCGCCGAAACGATAGCCTACAACTACAAAACCAGAATCGGCACGGCGTCGGATATGTCGTCGAAGAG
CGATCGGGATATCTATTCCGGAGGGAGCGATCAGGCTATCTATTCAGGCAAGGAGGTCAAGCGTATGCCCTCCGGAGAAC
TTTATATCGAGGATGGTGTCTATACGACCTGCGACCTCGAAGAGCCGCACTACTGGTTCGCGGGCAAGCACATGAAAATC
ATTCCGGGAGAACGCCTCATTTCCCGGCCGTTCGTGATGTACATTCATCCGGAAATCTTCCACATGCGCCTGCCGGTGTT
GCCGGTCATGTATTTGCCCTACATGTCGGCCCCGATCTCCAACAAGCGCGCCTCTGGCTTTCTGTTTCCGAGGTTCGGCA
ACAGTGGCGACATGGGCAGTTACTTTTCGAATCTTGGCTACTTCTGGGCGATTAACGATTATGCAGACCTGCGGCTTGAC
GGCGATATTGCGTTCAAGGGAGGCTGGCGGCTTGGTGAGCGGTTCCGTTACAAAAACGGTGATCGCTACAGCGGTTCGAT
TTCAGGAGAGTATGCCAGGATTATTCTGAACAGCCCCGGCGATTCCAATTATGCCCGATACATCAACCGGGATTTACGGA
TCGAGCATCACCAGCAGTTCGACCCTACCGCCGTGCTCGATGTCAATCTTCAGTTTCTGGGCGGCGATCGCTACTATTAC
GGCTACACCTCGGTCGATCCTGAAAACCTCGTGACCGATCAGGCGACTTCGTATGCATCGTTCACAAAATCTTGGGACGA
GAACAACCGGGTGCTGCTTGGCGGCTACCAGCGGGTGGACAATCTCTCCACCGACGAGCTGACCCAGAGGGTGACCCTGT
CGCTCTACCAGAACCGCATCTACCCGTTCCGCCCGAGGCTCTCTTCATCGTCATCGGAATCACCTGGCTGGAGTTCGCGC
CTATTTGTGCAGCCGACCCTGTCAGGCAGCGGTCAGTTCGATGCTGCTGGCGGAGTCAATACCGATTTTTACACGGGCAA
TGCCGGGCTGGAGCTGGGCTATCTGCAAGATTTTTCGCCAGGTAACCGGGCGCTGTTTACACAGGGGCTCAACATGCAGG
CTCTTCGTAAAACAATAACCGGCGAGGATGACCTGAACGCCACCAGCGTGCAGCTTCCTTTCAAGATCCAGTCCACGCTG
TTCAAGTACCTGCACCTGACTCCGGCACTGACCTTTACCCAGTACCGCGTCAACAGCACGGTCAACAAGTATTACGACCA
CATCGCTGGCAAAGTGGTGACGCAAACCATCAACGATTCCGACAGTTACGCCACGACGGTCTTTTCGCTCGACGCGCAGA
CGCGCCTTTACGGGGTGATGAACACCGGTTTTCTCGACAAGCTTGTCGGCCTTACCGCCATCCGTCACGTTTTCATTCCG
ACCGTTTCGTTCATTTACAATCCCGATTATACGGGGAGCGGTTACGGTATTTACGGCTCCTATTACGATCCGGTGCAGAT
GAAAAATGTGCAGTACAACCGGTTCGGGGAGTCGCTTTACGCTGATGTGCCTGAAAAGCGGACCTTTGTCGGCCTGAGTC
TCCAGAACATTTTTCAGGGCAAGTTCCGGAGCAAAAAGGTGTCGAACGAAGATGGCAGTAACGCCGGAGCCGGATATAAA
ACGGTGCAACTGCTCTCCCTGACGGCCTCGTCCGGGTACAATTTTGCCGCCGACTCGTTTCCCATCGCGCCGCTGGTGCT
GACGGCGTCGAGCAATGCCTTTGCCCCCGCGCTGATGTTCAGCGCCGGTGCGACCTACGACTTCTATACCTACGATCCAG
CAACCGGCGATCGGGTGAATAAGCTCGCCATGGACGACGGCAAGGGCCTGCTCCGCTTTGTCAACGGGTTCCTCAACATG
AGCGTGAGCGTGAGCGGCAGCCTGCACACCTCGTATGCCTCCCATGATGAAAGGGATGGCGAGATGTCACTTGTCCGGGA
GAAGGCTTTGCCGGTCGAGCAGGCGATCTACAAGGAGCGGTTCAATAGCGATGAACGGACCAAATTCAGTGCATCGTTAC
CCTGGTCGCTACGCATGTCGCTTTATTTGATCAGCGACAAGAGTAATCCGCTTGATCCTTCCTCTGCTGCGCTGCTTAAC
ACCGCGGCCCGGCTCTCTCTTTCGAGAAACTGGCAGGTTGGCCTTAATACCGGCTACGATCTCAGAAACAGCGAATTCGT
CTATCCCGCGCTTATGCTCGACAGGGATTTGCACGATTTCTGGTTCAGCGCCCAGTGGGTACCATCGGGCGAGCACAAGG
GCTATCTGTTCCAGATCGCCATGAAACCGGCCAACCTCAAGTACCTGAAGCTGAAGGCGGGAAGCGGCCATATTGTTCAG
TCTCCGGAGTAG

Upstream 100 bases:

>100_bases
CCCGTTGCCGGATGTCGGTGCAGGGTATTTGTTACAAAATATAGAAACCTTTCGATCAATTCTCCATCTGCCGGAGCCAT
TCTCTTTCCGTTTCCGCGGC

Downstream 100 bases:

>100_bases
GCTGGGGCAGTATCAGCTACAGAACTCTGTTGTTACTCCAGCCGCTGGAGAAGCTGCGCGAGGCGGTTGTTGAAGGTCTC
GTGCGCATCGTGATGGATGC

Product: hypothetical protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 963; Mature: 962

Protein sequence:

>963_residues
MSCSGEVSIKEKGFIYSCSDLHDEHHQRPLTQHDPPVKFTKSIKLLTLLLLVQSFEGILPLRAGESASKPQKAKDGALPD
SLLEKREDLDSTVVYTARDSLIYNVSKRTADLFGKAKVNYKDSRIEGPRITIEQATSTARATASRDSLGRPAELPVYTGK
DGSFSAETIAYNYKTRIGTASDMSSKSDRDIYSGGSDQAIYSGKEVKRMPSGELYIEDGVYTTCDLEEPHYWFAGKHMKI
IPGERLISRPFVMYIHPEIFHMRLPVLPVMYLPYMSAPISNKRASGFLFPRFGNSGDMGSYFSNLGYFWAINDYADLRLD
GDIAFKGGWRLGERFRYKNGDRYSGSISGEYARIILNSPGDSNYARYINRDLRIEHHQQFDPTAVLDVNLQFLGGDRYYY
GYTSVDPENLVTDQATSYASFTKSWDENNRVLLGGYQRVDNLSTDELTQRVTLSLYQNRIYPFRPRLSSSSSESPGWSSR
LFVQPTLSGSGQFDAAGGVNTDFYTGNAGLELGYLQDFSPGNRALFTQGLNMQALRKTITGEDDLNATSVQLPFKIQSTL
FKYLHLTPALTFTQYRVNSTVNKYYDHIAGKVVTQTINDSDSYATTVFSLDAQTRLYGVMNTGFLDKLVGLTAIRHVFIP
TVSFIYNPDYTGSGYGIYGSYYDPVQMKNVQYNRFGESLYADVPEKRTFVGLSLQNIFQGKFRSKKVSNEDGSNAGAGYK
TVQLLSLTASSGYNFAADSFPIAPLVLTASSNAFAPALMFSAGATYDFYTYDPATGDRVNKLAMDDGKGLLRFVNGFLNM
SVSVSGSLHTSYASHDERDGEMSLVREKALPVEQAIYKERFNSDERTKFSASLPWSLRMSLYLISDKSNPLDPSSAALLN
TAARLSLSRNWQVGLNTGYDLRNSEFVYPALMLDRDLHDFWFSAQWVPSGEHKGYLFQIAMKPANLKYLKLKAGSGHIVQ
SPE

Sequences:

>Translated_963_residues
MSCSGEVSIKEKGFIYSCSDLHDEHHQRPLTQHDPPVKFTKSIKLLTLLLLVQSFEGILPLRAGESASKPQKAKDGALPD
SLLEKREDLDSTVVYTARDSLIYNVSKRTADLFGKAKVNYKDSRIEGPRITIEQATSTARATASRDSLGRPAELPVYTGK
DGSFSAETIAYNYKTRIGTASDMSSKSDRDIYSGGSDQAIYSGKEVKRMPSGELYIEDGVYTTCDLEEPHYWFAGKHMKI
IPGERLISRPFVMYIHPEIFHMRLPVLPVMYLPYMSAPISNKRASGFLFPRFGNSGDMGSYFSNLGYFWAINDYADLRLD
GDIAFKGGWRLGERFRYKNGDRYSGSISGEYARIILNSPGDSNYARYINRDLRIEHHQQFDPTAVLDVNLQFLGGDRYYY
GYTSVDPENLVTDQATSYASFTKSWDENNRVLLGGYQRVDNLSTDELTQRVTLSLYQNRIYPFRPRLSSSSSESPGWSSR
LFVQPTLSGSGQFDAAGGVNTDFYTGNAGLELGYLQDFSPGNRALFTQGLNMQALRKTITGEDDLNATSVQLPFKIQSTL
FKYLHLTPALTFTQYRVNSTVNKYYDHIAGKVVTQTINDSDSYATTVFSLDAQTRLYGVMNTGFLDKLVGLTAIRHVFIP
TVSFIYNPDYTGSGYGIYGSYYDPVQMKNVQYNRFGESLYADVPEKRTFVGLSLQNIFQGKFRSKKVSNEDGSNAGAGYK
TVQLLSLTASSGYNFAADSFPIAPLVLTASSNAFAPALMFSAGATYDFYTYDPATGDRVNKLAMDDGKGLLRFVNGFLNM
SVSVSGSLHTSYASHDERDGEMSLVREKALPVEQAIYKERFNSDERTKFSASLPWSLRMSLYLISDKSNPLDPSSAALLN
TAARLSLSRNWQVGLNTGYDLRNSEFVYPALMLDRDLHDFWFSAQWVPSGEHKGYLFQIAMKPANLKYLKLKAGSGHIVQ
SPE
>Mature_962_residues
SCSGEVSIKEKGFIYSCSDLHDEHHQRPLTQHDPPVKFTKSIKLLTLLLLVQSFEGILPLRAGESASKPQKAKDGALPDS
LLEKREDLDSTVVYTARDSLIYNVSKRTADLFGKAKVNYKDSRIEGPRITIEQATSTARATASRDSLGRPAELPVYTGKD
GSFSAETIAYNYKTRIGTASDMSSKSDRDIYSGGSDQAIYSGKEVKRMPSGELYIEDGVYTTCDLEEPHYWFAGKHMKII
PGERLISRPFVMYIHPEIFHMRLPVLPVMYLPYMSAPISNKRASGFLFPRFGNSGDMGSYFSNLGYFWAINDYADLRLDG
DIAFKGGWRLGERFRYKNGDRYSGSISGEYARIILNSPGDSNYARYINRDLRIEHHQQFDPTAVLDVNLQFLGGDRYYYG
YTSVDPENLVTDQATSYASFTKSWDENNRVLLGGYQRVDNLSTDELTQRVTLSLYQNRIYPFRPRLSSSSSESPGWSSRL
FVQPTLSGSGQFDAAGGVNTDFYTGNAGLELGYLQDFSPGNRALFTQGLNMQALRKTITGEDDLNATSVQLPFKIQSTLF
KYLHLTPALTFTQYRVNSTVNKYYDHIAGKVVTQTINDSDSYATTVFSLDAQTRLYGVMNTGFLDKLVGLTAIRHVFIPT
VSFIYNPDYTGSGYGIYGSYYDPVQMKNVQYNRFGESLYADVPEKRTFVGLSLQNIFQGKFRSKKVSNEDGSNAGAGYKT
VQLLSLTASSGYNFAADSFPIAPLVLTASSNAFAPALMFSAGATYDFYTYDPATGDRVNKLAMDDGKGLLRFVNGFLNMS
VSVSGSLHTSYASHDERDGEMSLVREKALPVEQAIYKERFNSDERTKFSASLPWSLRMSLYLISDKSNPLDPSSAALLNT
AARLSLSRNWQVGLNTGYDLRNSEFVYPALMLDRDLHDFWFSAQWVPSGEHKGYLFQIAMKPANLKYLKLKAGSGHIVQS
PE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 107608; Mature: 107477

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCSGEVSIKEKGFIYSCSDLHDEHHQRPLTQHDPPVKFTKSIKLLTLLLLVQSFEGILP
CCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
LRAGESASKPQKAKDGALPDSLLEKREDLDSTVVYTARDSLIYNVSKRTADLFGKAKVNY
EECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEECHHHHHHHHCCEEECC
KDSRIEGPRITIEQATSTARATASRDSLGRPAELPVYTGKDGSFSAETIAYNYKTRIGTA
CCCCCCCCEEEEECCCCHHHHHCCHHCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCCC
SDMSSKSDRDIYSGGSDQAIYSGKEVKRMPSGELYIEDGVYTTCDLEEPHYWFAGKHMKI
CCCCCCCCCHHHCCCCCCEEECCHHHHCCCCCCEEEECCEEEEECCCCCCEEECCCEEEE
IPGERLISRPFVMYIHPEIFHMRLPVLPVMYLPYMSAPISNKRASGFLFPRFGNSGDMGS
CCCCHHHCCCEEEEECCHHEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHH
YFSNLGYFWAINDYADLRLDGDIAFKGGWRLGERFRYKNGDRYSGSISGEYARIILNSPG
HHHCCCEEEEECCCEEEEECCCEEEECCCCCCCHHCCCCCCEECCCCCCCEEEEEEECCC
DSNYARYINRDLRIEHHQQFDPTAVLDVNLQFLGGDRYYYGYTSVDPENLVTDQATSYAS
CCHHHHHHCCCCEEECCCCCCCEEEEEEEEEEEECCEEEEECCCCCHHHHHHCCCHHHHH
FTKSWDENNRVLLGGYQRVDNLSTDELTQRVTLSLYQNRIYPFRPRLSSSSSESPGWSSR
HHHCCCCCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
LFVQPTLSGSGQFDAAGGVNTDFYTGNAGLELGYLQDFSPGNRALFTQGLNMQALRKTIT
EEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHC
GEDDLNATSVQLPFKIQSTLFKYLHLTPALTFTQYRVNSTVNKYYDHIAGKVVTQTINDS
CCCCCCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHEEEEECCCC
DSYATTVFSLDAQTRLYGVMNTGFLDKLVGLTAIRHVFIPTVSFIYNPDYTGSGYGIYGS
CCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECCC
YYDPVQMKNVQYNRFGESLYADVPEKRTFVGLSLQNIFQGKFRSKKVSNEDGSNAGAGYK
CCCCEEECCCCHHHHCCHHHCCCCCCCEEEEEEHHHHHHCHHHCCCCCCCCCCCCCCCCE
TVQLLSLTASSGYNFAADSFPIAPLVLTASSNAFAPALMFSAGATYDFYTYDPATGDRVN
EEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEECCCCEEEEEECCCCCCCHH
KLAMDDGKGLLRFVNGFLNMSVSVSGSLHTSYASHDERDGEMSLVREKALPVEQAIYKER
EEECCCCCHHHHHHHHHHEEEEEECCCEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
FNSDERTKFSASLPWSLRMSLYLISDKSNPLDPSSAALLNTAARLSLSRNWQVGLNTGYD
CCCCCCEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCEEEECCCCCC
LRNSEFVYPALMLDRDLHDFWFSAQWVPSGEHKGYLFQIAMKPANLKYLKLKAGSGHIVQ
CCCCCEEEEHHHHCCCHHHHHEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEC
SPE
CCC
>Mature Secondary Structure 
SCSGEVSIKEKGFIYSCSDLHDEHHQRPLTQHDPPVKFTKSIKLLTLLLLVQSFEGILP
CCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
LRAGESASKPQKAKDGALPDSLLEKREDLDSTVVYTARDSLIYNVSKRTADLFGKAKVNY
EECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEECHHHHHHHHCCEEECC
KDSRIEGPRITIEQATSTARATASRDSLGRPAELPVYTGKDGSFSAETIAYNYKTRIGTA
CCCCCCCCEEEEECCCCHHHHHCCHHCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCCC
SDMSSKSDRDIYSGGSDQAIYSGKEVKRMPSGELYIEDGVYTTCDLEEPHYWFAGKHMKI
CCCCCCCCCHHHCCCCCCEEECCHHHHCCCCCCEEEECCEEEEECCCCCCEEECCCEEEE
IPGERLISRPFVMYIHPEIFHMRLPVLPVMYLPYMSAPISNKRASGFLFPRFGNSGDMGS
CCCCHHHCCCEEEEECCHHEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHH
YFSNLGYFWAINDYADLRLDGDIAFKGGWRLGERFRYKNGDRYSGSISGEYARIILNSPG
HHHCCCEEEEECCCEEEEECCCEEEECCCCCCCHHCCCCCCEECCCCCCCEEEEEEECCC
DSNYARYINRDLRIEHHQQFDPTAVLDVNLQFLGGDRYYYGYTSVDPENLVTDQATSYAS
CCHHHHHHCCCCEEECCCCCCCEEEEEEEEEEEECCEEEEECCCCCHHHHHHCCCHHHHH
FTKSWDENNRVLLGGYQRVDNLSTDELTQRVTLSLYQNRIYPFRPRLSSSSSESPGWSSR
HHHCCCCCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
LFVQPTLSGSGQFDAAGGVNTDFYTGNAGLELGYLQDFSPGNRALFTQGLNMQALRKTIT
EEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHC
GEDDLNATSVQLPFKIQSTLFKYLHLTPALTFTQYRVNSTVNKYYDHIAGKVVTQTINDS
CCCCCCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHEEEEECCCC
DSYATTVFSLDAQTRLYGVMNTGFLDKLVGLTAIRHVFIPTVSFIYNPDYTGSGYGIYGS
CCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECCC
YYDPVQMKNVQYNRFGESLYADVPEKRTFVGLSLQNIFQGKFRSKKVSNEDGSNAGAGYK
CCCCEEECCCCHHHHCCHHHCCCCCCCEEEEEEHHHHHHCHHHCCCCCCCCCCCCCCCCE
TVQLLSLTASSGYNFAADSFPIAPLVLTASSNAFAPALMFSAGATYDFYTYDPATGDRVN
EEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEECCCCEEEEEECCCCCCCHH
KLAMDDGKGLLRFVNGFLNMSVSVSGSLHTSYASHDERDGEMSLVREKALPVEQAIYKER
EEECCCCCHHHHHHHHHHEEEEEECCCEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
FNSDERTKFSASLPWSLRMSLYLISDKSNPLDPSSAALLNTAARLSLSRNWQVGLNTGYD
CCCCCCEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCEEEECCCCCC
LRNSEFVYPALMLDRDLHDFWFSAQWVPSGEHKGYLFQIAMKPANLKYLKLKAGSGHIVQ
CCCCCEEEEHHHHCCCHHHHHEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEC
SPE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA