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The map label for this gene is trpB-1

Identifier: 21673356

GI number: 21673356

Start: 527030

End: 528232

Strand: Reverse

Name: trpB-1

Synonym: CT0521

Alternate gene names: 21673356

Gene position: 528232-527030 (Counterclockwise)

Preceding gene: 21673357

Following gene: 21673355

Centisome position: 24.51

GC content: 61.35

Gene sequence:

>1203_bases
ATGAAGCAGAAGGTTATCTACTCCGCACCCGATGAATTCGGCCACTTCGGCACCTTCGGAGGCAAATTCATTCCCGAAAC
CCTGGTCAAAAACGCCGCCGATCTCGAAGAGGAGTACCTCAAGGCGAAAAATGATCCGGAGTTCCACCAAACGCTCGACA
ACCTGCTTCGCCACTACGTTGGCCGCCCGACGCCGCTCTATCACGCCTCGCGGCTCAGCGAAAAGCAGGGCGGCGCCCAG
ATCTGGCTCAAGCGCGAAGACCTCTGCCACACCGGCGCGCACAAGATCAACAACGCCCTCGGCCAGGTGCTGCTGGCCAA
ACGGATGGGCAAAAAGCGCATCATCGCCGAAACCGGCGCGGGCCAGCATGGCGTGGCAACGGCCACCGTCTGCGCGCTCT
TCGGTCTCGACTGCATCGTCTATATGGGCGAAGAGGACATCCGCCGCCAGGCTCCCAACGTGGCGCGCATGAAGCTGCTC
GGCACAGAGGTGCGCCCGGTCACGGCAGGCAGCAGAACGCTGAAGGACGCCACCAGCGAGGCAATCCGCGACTGGATGAA
CAATCCCGAAGAGACCTTCTACATCGTTGGCTCGGTGATCGGAATGCACCCGTACCCGATGATGGTACGCGACTTCCAGT
CGGTCATCGGACGCGAAACCCGCCAGCAGGTGCTCGATCAGGCCGGGCGCTTGCCCGATGTGATCGTGGCCTGCGTCGGC
GGCGGCAGCAACGCCATCGGCATGTTCTACGAGTTTTTGCCGGATGCCAAAGAGGTCGAACTGATCGGCGTCGAAGCCGC
CGGAGAGGGACTCGAAGGCAAGCACGCCGCGTCGCTGACAAAGGGTGAAATCGGCGTGCTGCACGGCTCGATGATGAAGC
TCTTGCAGGACGAGTACGGTCAGGTGCAGGAGGCGCACTCGATTTCGGCGGGACTCGACTACCCCGGCGTCGGTCCGGAG
CACTGCTATTTGCAGAAGCTCGGCCTGGTCTGCTATACCTCGACGACAGACAAGGAAGCCCTCGCAGCGCTCGACGCTCT
GGCCAAAACCGAAGGCATCATCTGCGCCCTCGAATCGGCTCACGCCGTGCATTATGCGATGAAACGAGCCGCCGAAATGC
CGAAAGAGTCGATCATCGTGGTCAACCTCTCTGGCCGCGGCGACAAAGACATGGGTACCATCATGCAGGAGCTGAAGCTG
TAA

Upstream 100 bases:

>100_bases
TCCGGCAACGGGCAAAAAGATTCACAACAGCTACAGGAAGTCGCCGCACTTTAGCTCGCAACAGAACGGCAACGCCATCT
TTAACACTTTGTAAGGCTTT

Downstream 100 bases:

>100_bases
GGATGAACAGCAGGGGCAGCCTTCGCGGCTGCCCGGCATCGGGTGGCACAGGAGGAAATCTTATCGGTCAAAAAAATTTT
CACATTTCTGTTGCGTAGAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKQKVIYSAPDEFGHFGTFGGKFIPETLVKNAADLEEEYLKAKNDPEFHQTLDNLLRHYVGRPTPLYHASRLSEKQGGAQ
IWLKREDLCHTGAHKINNALGQVLLAKRMGKKRIIAETGAGQHGVATATVCALFGLDCIVYMGEEDIRRQAPNVARMKLL
GTEVRPVTAGSRTLKDATSEAIRDWMNNPEETFYIVGSVIGMHPYPMMVRDFQSVIGRETRQQVLDQAGRLPDVIVACVG
GGSNAIGMFYEFLPDAKEVELIGVEAAGEGLEGKHAASLTKGEIGVLHGSMMKLLQDEYGQVQEAHSISAGLDYPGVGPE
HCYLQKLGLVCYTSTTDKEALAALDALAKTEGIICALESAHAVHYAMKRAAEMPKESIIVVNLSGRGDKDMGTIMQELKL

Sequences:

>Translated_400_residues
MKQKVIYSAPDEFGHFGTFGGKFIPETLVKNAADLEEEYLKAKNDPEFHQTLDNLLRHYVGRPTPLYHASRLSEKQGGAQ
IWLKREDLCHTGAHKINNALGQVLLAKRMGKKRIIAETGAGQHGVATATVCALFGLDCIVYMGEEDIRRQAPNVARMKLL
GTEVRPVTAGSRTLKDATSEAIRDWMNNPEETFYIVGSVIGMHPYPMMVRDFQSVIGRETRQQVLDQAGRLPDVIVACVG
GGSNAIGMFYEFLPDAKEVELIGVEAAGEGLEGKHAASLTKGEIGVLHGSMMKLLQDEYGQVQEAHSISAGLDYPGVGPE
HCYLQKLGLVCYTSTTDKEALAALDALAKTEGIICALESAHAVHYAMKRAAEMPKESIIVVNLSGRGDKDMGTIMQELKL
>Mature_400_residues
MKQKVIYSAPDEFGHFGTFGGKFIPETLVKNAADLEEEYLKAKNDPEFHQTLDNLLRHYVGRPTPLYHASRLSEKQGGAQ
IWLKREDLCHTGAHKINNALGQVLLAKRMGKKRIIAETGAGQHGVATATVCALFGLDCIVYMGEEDIRRQAPNVARMKLL
GTEVRPVTAGSRTLKDATSEAIRDWMNNPEETFYIVGSVIGMHPYPMMVRDFQSVIGRETRQQVLDQAGRLPDVIVACVG
GGSNAIGMFYEFLPDAKEVELIGVEAAGEGLEGKHAASLTKGEIGVLHGSMMKLLQDEYGQVQEAHSISAGLDYPGVGPE
HCYLQKLGLVCYTSTTDKEALAALDALAKTEGIICALESAHAVHYAMKRAAEMPKESIIVVNLSGRGDKDMGTIMQELKL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=387, Percent_Identity=53.2299741602067, Blast_Score=413, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=55.8441558441558, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_CHLTE (Q8KF11)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661421.1
- ProteinModelPortal:   Q8KF11
- SMR:   Q8KF11
- GeneID:   1006194
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0521
- NMPDR:   fig|194439.1.peg.515
- TIGR:   CT0521
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   CTEP194439:CT_0521-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43640; Mature: 43640

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKVIYSAPDEFGHFGTFGGKFIPETLVKNAADLEEEYLKAKNDPEFHQTLDNLLRHYV
CCCCEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GRPTPLYHASRLSEKQGGAQIWLKREDLCHTGAHKINNALGQVLLAKRMGKKRIIAETGA
CCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GQHGVATATVCALFGLDCIVYMGEEDIRRQAPNVARMKLLGTEVRPVTAGSRTLKDATSE
CCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCCHHHHHHHCCCCCEECCCCHHHHHHHHH
AIRDWMNNPEETFYIVGSVIGMHPYPMMVRDFQSVIGRETRQQVLDQAGRLPDVIVACVG
HHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEC
GGSNAIGMFYEFLPDAKEVELIGVEAAGEGLEGKHAASLTKGEIGVLHGSMMKLLQDEYG
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
QVQEAHSISAGLDYPGVGPEHCYLQKLGLVCYTSTTDKEALAALDALAKTEGIICALESA
HHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHH
HAVHYAMKRAAEMPKESIIVVNLSGRGDKDMGTIMQELKL
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKQKVIYSAPDEFGHFGTFGGKFIPETLVKNAADLEEEYLKAKNDPEFHQTLDNLLRHYV
CCCCEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GRPTPLYHASRLSEKQGGAQIWLKREDLCHTGAHKINNALGQVLLAKRMGKKRIIAETGA
CCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GQHGVATATVCALFGLDCIVYMGEEDIRRQAPNVARMKLLGTEVRPVTAGSRTLKDATSE
CCCCHHHHHHHHHHHHHHEEEECCHHHHHCCCCHHHHHHHCCCCCEECCCCHHHHHHHHH
AIRDWMNNPEETFYIVGSVIGMHPYPMMVRDFQSVIGRETRQQVLDQAGRLPDVIVACVG
HHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEC
GGSNAIGMFYEFLPDAKEVELIGVEAAGEGLEGKHAASLTKGEIGVLHGSMMKLLQDEYG
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
QVQEAHSISAGLDYPGVGPEHCYLQKLGLVCYTSTTDKEALAALDALAKTEGIICALESA
HHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHH
HAVHYAMKRAAEMPKESIIVVNLSGRGDKDMGTIMQELKL
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901