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The map label for this gene is nolF [H]

Identifier: 21673300

GI number: 21673300

Start: 482537

End: 483628

Strand: Direct

Name: nolF [H]

Synonym: CT0464

Alternate gene names: 21673300

Gene position: 482537-483628 (Clockwise)

Preceding gene: 21673297

Following gene: 21673302

Centisome position: 22.39

GC content: 59.34

Gene sequence:

>1092_bases
ATGCAGAATTTGCAGAAGATGCTTCCGAAGTTCTCGATAGCCCTGGCCGTGGTGTTCGCCGCGACCATCGGCTACTTGCT
GACCCGGGGCAATACCGTCGATGTCGAGGCGCGCAAAATTTCGAGATCGGAACTGGTCGAGGCGATCTATGCAACTGGCT
ACGTCGAGGCGGAGAACATCGCCAACCTGAGGGCCGAGTTCTCCGGGACGGTTCGCTCCATCGGCGCGCTCGAAGGGCAA
CGGGTCTCGAAAGGGCAGGCGATCATCGTGTTCGACAGCGTGCAGCCGCGCCTGGCCGTCAATGAGGCGCGCGCTGCCGT
GGCTGAAGAGATGGCCGCCGTTCACGATAACGACCTCCGGCTACAGCGGAACCGTACGCTGTTTCAGGCTGGCGCGATTT
CCCGTCAGGATTTCGAGGCGGCGGAACGCAACAGCACGGCGTCCCGCGAAGCGCTCCGGCAGCGCCAGATGCAGCTCAAG
AGCCGCGAGGACGATCTGAAAAAGCTGGAGGTTGTTGCACCGTTCAGCGGAATTCTGACACTTCAGAATGTCAAGGAGGG
CGATTACGTCCAGTCCGGCACGCTTGTGGCGACCGTGACCGATTCATCCCGCTACCTCGTGGTGGTTGAAGTTGACGAAC
TCGATGTGCCGCGTCTGCGCACCGGTCTCAAGGCGGTGATCGCTTTCGACTCGATGCCCGAGAAGCGTTTCGATGCCACC
GTCGTGCGCATTGTGCCGCAGACTGACCGGGTAACGAAAACTTCGCGCGTCTATCTGAAACTCGATGATTCGGTGGCGGC
GATCCAGGGCGGCATGACCGCAACGGCCAACATCATTTACAATACGAAGAAGGGAACGCTGCTGGTCAGCAAAAGCTCCG
TGTTCGAGGAGGAGCACCAGAGCTACGTCTGGAAAATTGTCAAGGGCAAGCTGAAAAAGCAGCCGATTCGCACCGGCGAC
AGCGATCTTGTGTTCATCGAGGTGGTCAAGGGGCTCAATGCCGGGGATGTGGTTGTCACTTCACCGCAGGAAAACTACCG
CGACGGCATGGAGGCGCGGATTGTCAGGGAGTCTCCGAAAAAGCAGTCGTAA

Upstream 100 bases:

>100_bases
GGGAAAAAATCACCGGTTTTCCGGCTCGCTCTTTTTGAAAAACTGTTTGGCCTCGTCCGCCGGGGCGATGTGTAACCGTT
AATTCCCATTCCCTGACGAT

Downstream 100 bases:

>100_bases
GCGTCATCCGTGAGCGCGAAGCGAATGTCCGTGATGCTGGCAGCCCATGCCGAGGCTGAAACCACCGGTTTCTTTGAGAA
CTACCAGGTGAGCCTGCATA

Product: membrane fusion protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQNLQKMLPKFSIALAVVFAATIGYLLTRGNTVDVEARKISRSELVEAIYATGYVEAENIANLRAEFSGTVRSIGALEGQ
RVSKGQAIIVFDSVQPRLAVNEARAAVAEEMAAVHDNDLRLQRNRTLFQAGAISRQDFEAAERNSTASREALRQRQMQLK
SREDDLKKLEVVAPFSGILTLQNVKEGDYVQSGTLVATVTDSSRYLVVVEVDELDVPRLRTGLKAVIAFDSMPEKRFDAT
VVRIVPQTDRVTKTSRVYLKLDDSVAAIQGGMTATANIIYNTKKGTLLVSKSSVFEEEHQSYVWKIVKGKLKKQPIRTGD
SDLVFIEVVKGLNAGDVVVTSPQENYRDGMEARIVRESPKKQS

Sequences:

>Translated_363_residues
MQNLQKMLPKFSIALAVVFAATIGYLLTRGNTVDVEARKISRSELVEAIYATGYVEAENIANLRAEFSGTVRSIGALEGQ
RVSKGQAIIVFDSVQPRLAVNEARAAVAEEMAAVHDNDLRLQRNRTLFQAGAISRQDFEAAERNSTASREALRQRQMQLK
SREDDLKKLEVVAPFSGILTLQNVKEGDYVQSGTLVATVTDSSRYLVVVEVDELDVPRLRTGLKAVIAFDSMPEKRFDAT
VVRIVPQTDRVTKTSRVYLKLDDSVAAIQGGMTATANIIYNTKKGTLLVSKSSVFEEEHQSYVWKIVKGKLKKQPIRTGD
SDLVFIEVVKGLNAGDVVVTSPQENYRDGMEARIVRESPKKQS
>Mature_363_residues
MQNLQKMLPKFSIALAVVFAATIGYLLTRGNTVDVEARKISRSELVEAIYATGYVEAENIANLRAEFSGTVRSIGALEGQ
RVSKGQAIIVFDSVQPRLAVNEARAAVAEEMAAVHDNDLRLQRNRTLFQAGAISRQDFEAAERNSTASREALRQRQMQLK
SREDDLKKLEVVAPFSGILTLQNVKEGDYVQSGTLVATVTDSSRYLVVVEVDELDVPRLRTGLKAVIAFDSMPEKRFDAT
VVRIVPQTDRVTKTSRVYLKLDDSVAAIQGGMTATANIIYNTKKGTLLVSKSSVFEEEHQSYVWKIVKGKLKKQPIRTGD
SDLVFIEVVKGLNAGDVVVTSPQENYRDGMEARIVRESPKKQS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=353, Percent_Identity=25.2124645892351, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082036, Length=312, Percent_Identity=21.7948717948718, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40153; Mature: 40153

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLQKMLPKFSIALAVVFAATIGYLLTRGNTVDVEARKISRSELVEAIYATGYVEAENI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCEEHHHH
ANLRAEFSGTVRSIGALEGQRVSKGQAIIVFDSVQPRLAVNEARAAVAEEMAAVHDNDLR
HHHHHHHCCHHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LQRNRTLFQAGAISRQDFEAAERNSTASREALRQRQMQLKSREDDLKKLEVVAPFSGILT
EECCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCEEE
LQNVKEGDYVQSGTLVATVTDSSRYLVVVEVDELDVPRLRTGLKAVIAFDSMPEKRFDAT
EECCCCCCEEECCEEEEEEECCCEEEEEEEECCCCCHHHHHHHHEEEEECCCCCCCCCCE
VVRIVPQTDRVTKTSRVYLKLDDSVAAIQGGMTATANIIYNTKKGTLLVSKSSVFEEEHQ
EEEEECCCCCCCEEEEEEEEECCCHHHHHCCCEEEEEEEEECCCCEEEEECHHHHHHHHH
SYVWKIVKGKLKKQPIRTGDSDLVFIEVVKGLNAGDVVVTSPQENYRDGMEARIVRESPK
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCCHHHHCCCCEEEECCCCC
KQS
CCC
>Mature Secondary Structure
MQNLQKMLPKFSIALAVVFAATIGYLLTRGNTVDVEARKISRSELVEAIYATGYVEAENI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCEEHHHH
ANLRAEFSGTVRSIGALEGQRVSKGQAIIVFDSVQPRLAVNEARAAVAEEMAAVHDNDLR
HHHHHHHCCHHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LQRNRTLFQAGAISRQDFEAAERNSTASREALRQRQMQLKSREDDLKKLEVVAPFSGILT
EECCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCEEE
LQNVKEGDYVQSGTLVATVTDSSRYLVVVEVDELDVPRLRTGLKAVIAFDSMPEKRFDAT
EECCCCCCEEECCEEEEEEECCCEEEEEEEECCCCCHHHHHHHHEEEEECCCCCCCCCCE
VVRIVPQTDRVTKTSRVYLKLDDSVAAIQGGMTATANIIYNTKKGTLLVSKSSVFEEEHQ
EEEEECCCCCCCEEEEEEEEECCCHHHHHCCCEEEEEEEEECCCCEEEEECHHHHHHHHH
SYVWKIVKGKLKKQPIRTGDSDLVFIEVVKGLNAGDVVVTSPQENYRDGMEARIVRESPK
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCCHHHHCCCCEEEECCCCC
KQS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]