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The map label for this gene is lig [H]

Identifier: 21673293

GI number: 21673293

Start: 477055

End: 479079

Strand: Direct

Name: lig [H]

Synonym: CT0457

Alternate gene names: 21673293

Gene position: 477055-479079 (Clockwise)

Preceding gene: 21673292

Following gene: 21673294

Centisome position: 22.14

GC content: 62.57

Gene sequence:

>2025_bases
ATGGATAAAGCGAAAGCACAACAGGAAATCGGCAAGCTTCGCGCGGAGATCGAACGCCACAATCGCCTCTACTACCTCGA
AGCCAAACCGGAGATTTCGGATTTCGAGTTCGACAAGCTGCTTAAACGGTTGATTATGCTCGAAAAGGAGTTCCCGGAGC
TGGTGACGCCCGACAGCCCGTCGCAGCGTGTTGGCGGCGGCATCACCAAGGAGTTTCCGACGGTTGTGCACCGCGACCCG
ATGCTCAGTCTTTCGAACACCTACTCCATCGCGGAGGTGGCCGACTTCTGCAATCGCGTCGAAAAGCTGGTGGCAGCCGA
GGGCGGCGGTACGCCGGAGTACGTCGCCGAGCTGAAGTATGACGGTGTGGCGATCAGCTTGCTCTACCGCGACGGTCTGC
TCGTTTGCGGCTCCACGCGTGGCGACGGTCGGCAAGGCGACGAGATCACGGCCAATCTCCGGACAATCCCGTCGATTCCG
CTTCGGCTCGAAATGCCCGATCTGCCGCTTTTCGGCACAGCATTGAGCGGCGAAATCGAGGTGCGCGGCGAGGTCTATAT
GCGCAAGGACGATTTCGAGCGGCTCAACGAGGAGCGCCCGGAGGAGGAGCGTTTCGCCAACCCGCGCAACGCCACCGCCG
GCACGCTCAAGTTGCAGGACTCGGCGGAGGTCGCGCGGCGCCGGATGTCGTTCGTGGCCTACTATCTCAAGGGGTACGAC
GGCGAAGCGCCCACGCACCTGAAGCGGCTGGAGCAGCTCAAAAGCATGGGCTTCATGACCGGCGCGGCGGCTAAGCTTTG
CAAAGGGATGGACGAGATTGCCGACTTCATCGCCGAGTGGTCGGAGAAGCGCTGGACGCTGCCGTACGAAACCGACGGCG
TGGTGCTCAAGCTCAACGAGGTCAGCCTGTGGGAGCGGCTCGGCGCGACCGCCAAAAGTCCGCGCTGGGCGATTGCCTAC
AAGTACCCGGCGCAGCAGGCCAAGACCGTGCTGCAAGGCGTGGTGTTCCAGGTCGGGCGGCTCGGCACCATCACGCCGGT
GGCCGAACTCAAGCCGACCAGGCTCGCTGGATCGATCGTGTCGCGCTCGACCCTGCACAACTTCGACGAAATCAAACGGC
TTGGCGTGCGCATTGGCGACCACGTTATGATCGAAAAGTCGGGCGAAGTAATTCCGAAAGTGGTCAGCGTCGTGCTCGAC
GAACGCCCGGCGGAAACCGCCGAAATCGAAGTGCCGTCGGAGTGCCCGGTTTGCGGCACCAGGCTGGAGCGGCCCGAAGG
CGAGGTGAACTGGTATTGCCCCAACGAGGAGGGATGCCCCGCGCAGAAGCGAGGCCGCATTCTGCACTTCGCCTCGCGCA
ACGCCCTCGACATCCAGAACCTCGGCGAGTCGCTTGTTACGCAGCTCGTCGATCGTGGCCTCGTGAGCGACGCGGGCGAC
CTTTACAGTCTTACTCAGGAGCAGCTCGCGGGCCTCGACCGCATGGCTGCCAAATCGGCGCAAAACGTGCTCGACGCGCT
CGAAAAGAGCAAAAAGCAGAGCTACGCCCGCCTGCTCTTCGCCCTCGGCATCCGCCACGTCGGCGCAGCCACGGCCCGCG
AACTGGCCCACGCCTGCCCCTCCATCGACCGCCTCCGCGAGATGGATGAAGAGGCGCTCGCCGCCGTGCCCGACATCGGC
CCCGTCATCGCCGCAAGCATCCGCGACTTCTTCGCCAAACCGTGGGTGCAAGCGATGCTCCAAAAGCTCGCCGAAGCCGG
GCTGCCGATGCAGGCGGGAGAGGAAAAGGCGCTCGTTAACAACAATTTTGAAGGACAAAGCGTCATCTTCACCGGCGCAC
TGGAACGCCACGTCCGCCAGGAGGCCGAAGAGATGGTACGCGAACGCGGCGGCAGAATCGTCAGCTCGGTGAGCAAAAAG
ACAACACTCGTTGTCGCAGGCAAAGAGGCCGGAAGCAAGCTTGAAAAGGCGATAAAGCTGGGAGTGAAGGTGATTGACGA
GGATGAGTTCGAGCGGATGCTGTGA

Upstream 100 bases:

>100_bases
AGGCGACCTGGAACAAACTCGGCGTGGGCGGGTCGGTCGGCTTCGTGAAAAACCTTGCCGGATGGGCAATATCGCAGAAC
GAAAAACGCGGTTAACATCG

Downstream 100 bases:

>100_bases
GGGTGGATGTACGGTTATAAAATCAGAATCAGGTTCTTCGCAGAATATCGTGGGTAAATGGTGAATATGGGTCAATCGAC
TGAGGCACGGCCAAAACGAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLKRLIMLEKEFPELVTPDSPSQRVGGGITKEFPTVVHRDP
MLSLSNTYSIAEVADFCNRVEKLVAAEGGGTPEYVAELKYDGVAISLLYRDGLLVCGSTRGDGRQGDEITANLRTIPSIP
LRLEMPDLPLFGTALSGEIEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGYD
GEAPTHLKRLEQLKSMGFMTGAAAKLCKGMDEIADFIAEWSEKRWTLPYETDGVVLKLNEVSLWERLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTRLAGSIVSRSTLHNFDEIKRLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEVPSECPVCGTRLERPEGEVNWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVTQLVDRGLVSDAGD
LYSLTQEQLAGLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMDEEALAAVPDIG
PVIAASIRDFFAKPWVQAMLQKLAEAGLPMQAGEEKALVNNNFEGQSVIFTGALERHVRQEAEEMVRERGGRIVSSVSKK
TTLVVAGKEAGSKLEKAIKLGVKVIDEDEFERML

Sequences:

>Translated_674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLKRLIMLEKEFPELVTPDSPSQRVGGGITKEFPTVVHRDP
MLSLSNTYSIAEVADFCNRVEKLVAAEGGGTPEYVAELKYDGVAISLLYRDGLLVCGSTRGDGRQGDEITANLRTIPSIP
LRLEMPDLPLFGTALSGEIEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGYD
GEAPTHLKRLEQLKSMGFMTGAAAKLCKGMDEIADFIAEWSEKRWTLPYETDGVVLKLNEVSLWERLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTRLAGSIVSRSTLHNFDEIKRLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEVPSECPVCGTRLERPEGEVNWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVTQLVDRGLVSDAGD
LYSLTQEQLAGLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMDEEALAAVPDIG
PVIAASIRDFFAKPWVQAMLQKLAEAGLPMQAGEEKALVNNNFEGQSVIFTGALERHVRQEAEEMVRERGGRIVSSVSKK
TTLVVAGKEAGSKLEKAIKLGVKVIDEDEFERML
>Mature_674_residues
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLKRLIMLEKEFPELVTPDSPSQRVGGGITKEFPTVVHRDP
MLSLSNTYSIAEVADFCNRVEKLVAAEGGGTPEYVAELKYDGVAISLLYRDGLLVCGSTRGDGRQGDEITANLRTIPSIP
LRLEMPDLPLFGTALSGEIEVRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGYD
GEAPTHLKRLEQLKSMGFMTGAAAKLCKGMDEIADFIAEWSEKRWTLPYETDGVVLKLNEVSLWERLGATAKSPRWAIAY
KYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTRLAGSIVSRSTLHNFDEIKRLGVRIGDHVMIEKSGEVIPKVVSVVLD
ERPAETAEIEVPSECPVCGTRLERPEGEVNWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVTQLVDRGLVSDAGD
LYSLTQEQLAGLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACPSIDRLREMDEEALAAVPDIG
PVIAASIRDFFAKPWVQAMLQKLAEAGLPMQAGEEKALVNNNFEGQSVIFTGALERHVRQEAEEMVRERGGRIVSSVSKK
TTLVVAGKEAGSKLEKAIKLGVKVIDEDEFERML

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=678, Percent_Identity=43.8053097345133, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=532, Percent_Identity=25.3759398496241, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74785; Mature: 74785

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLKRLIMLEKEFPELVTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGGITKEFPTVVHRDPMLSLSNTYSIAEVADFCNRVEKLVAAEGGGTPEYVAELKY
CHHHCCCCCHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
DGVAISLLYRDGLLVCGSTRGDGRQGDEITANLRTIPSIPLRLEMPDLPLFGTALSGEIE
CCEEEEEEEECCEEEEECCCCCCCCCCCEEECCCCCCCCCEEEECCCCCEECCCCCCCEE
VRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGYD
ECCEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
GEAPTHLKRLEQLKSMGFMTGAAAKLCKGMDEIADFIAEWSEKRWTLPYETDGVVLKLNE
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECH
VSLWERLGATAKSPRWAIAYKYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTRLAGSIV
HHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
SRSTLHNFDEIKRLGVRIGDHVMIEKSGEVIPKVVSVVLDERPAETAEIEVPSECPVCGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
RLERPEGEVNWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVTQLVDRGLVSDAGD
CCCCCCCCEEEECCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCCHH
LYSLTQEQLAGLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIDRLREMDEEALAAVPDIGPVIAASIRDFFAKPWVQAMLQKLAEAGLPMQAGEEKALVN
CHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHC
NNFEGQSVIFTGALERHVRQEAEEMVRERGGRIVSSVSKKTTLVVAGKEAGSKLEKAIKL
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
GVKVIDEDEFERML
CCHHCCCHHHHHCC
>Mature Secondary Structure
MDKAKAQQEIGKLRAEIERHNRLYYLEAKPEISDFEFDKLLKRLIMLEKEFPELVTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGGITKEFPTVVHRDPMLSLSNTYSIAEVADFCNRVEKLVAAEGGGTPEYVAELKY
CHHHCCCCCHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
DGVAISLLYRDGLLVCGSTRGDGRQGDEITANLRTIPSIPLRLEMPDLPLFGTALSGEIE
CCEEEEEEEECCEEEEECCCCCCCCCCCEEECCCCCCCCCEEEECCCCCEECCCCCCCEE
VRGEVYMRKDDFERLNEERPEEERFANPRNATAGTLKLQDSAEVARRRMSFVAYYLKGYD
ECCEEEEECHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
GEAPTHLKRLEQLKSMGFMTGAAAKLCKGMDEIADFIAEWSEKRWTLPYETDGVVLKLNE
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECH
VSLWERLGATAKSPRWAIAYKYPAQQAKTVLQGVVFQVGRLGTITPVAELKPTRLAGSIV
HHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
SRSTLHNFDEIKRLGVRIGDHVMIEKSGEVIPKVVSVVLDERPAETAEIEVPSECPVCGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
RLERPEGEVNWYCPNEEGCPAQKRGRILHFASRNALDIQNLGESLVTQLVDRGLVSDAGD
CCCCCCCCEEEECCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCCHH
LYSLTQEQLAGLDRMAAKSAQNVLDALEKSKKQSYARLLFALGIRHVGAATARELAHACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIDRLREMDEEALAAVPDIGPVIAASIRDFFAKPWVQAMLQKLAEAGLPMQAGEEKALVN
CHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHC
NNFEGQSVIFTGALERHVRQEAEEMVRERGGRIVSSVSKKTTLVVAGKEAGSKLEKAIKL
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHH
GVKVIDEDEFERML
CCHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA