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The map label for this gene is gltD [H]

Identifier: 21673241

GI number: 21673241

Start: 418191

End: 419663

Strand: Direct

Name: gltD [H]

Synonym: CT0402

Alternate gene names: 21673241

Gene position: 418191-419663 (Clockwise)

Preceding gene: 21673240

Following gene: 21673242

Centisome position: 19.41

GC content: 60.69

Gene sequence:

>1473_bases
ATGGGTAAACTGAAAGGATTTATGGAGTACCGGAGAGCCCTTCCCGTAGACCGGGAGCCTCTGGAAAGAATAAAGGACTG
GAACGAGTTCCACGAGGAGATGTCGGCCGAGCAGCTCAGCGACCAGGGCGCGCGCTGCATGGATTGTGGCACGCCGTTCT
GCCACTCGGGCTTCATGCTCAACGGCATGACTGCTGGCTGCCCGATTCACAACCTTATCCCCGAGTGGAACGATCACGTC
TATCGCGGCTTCTGGCGCGACGCCTGGGAACGACTGATGAAGACCAACAACTTCCCGGAATTCACCGGCAGGGTGTGCCC
CGCGCCGTGTGAAGGCTCCTGCGTGCTCGGCATCATCCAGCCGCCGGTGACCATCAAGAATATCGAGTACTCGATCATCG
AACATGCCTTCGCTGAAGGGTGGGTCGAGCCGAAGCAGATCGCCGTCCGCACTGGTAAAAAGGTGGCTATTGTGGGTTCC
GGTCCGTCGGGCCTTGCCTGCGCCGACCAGCTCAACAAGGCGGGGCACACGGTCACGGTTTTCGAGCGTGATGACCGCGT
TGGCGGCCTTCTCATGTACGGTATTCCGAACATGAAGCTCGACAAGCGGCTGGTGGTGCAGCGCCGCGTCGATCTCATGA
AAGAAGAGGGCGTGAGTTTTGTGACCGGAACCGAAGTTGGAGTCAACTACCCGGTTGACAAGCTGCTCTCGGAGTACGAC
GCCGTGGTGCTCTGTATCGGAGCGACCAACCCGCGCGATCTCAATGCCGATGGGCGCAATCTTGACGGCATTCACTTCGC
GATGGAGTTTCTCCGTGCCAGCACCAAGGCTGTGCTCGATGGCACCGAACCTGTGCTCTCCGCCAAGGGCAAGGATGTGG
TGGTGATCGGCGGCGGTGACACCGGAACCGACTGCGTGGCTACATCGCTGCGCCAGGGGTGTAAGAGCGTCATACAGCTT
GAAATCATGCCGAAACCAGCCGACTTCCGCCAAGAAGACAACCCGTGGCCCGAATGGCCGAAGGTTTTCAAGGTCGATTA
CGGCCAGGAAGAGGCAGCTGCCGTGCAGGGCGGCGACCCGCGTCGCTATCTCATGATGACCAAGAAGTTCATCGGCGAGA
ACGGGCGGCTCAGTGCCGTCGAGGTCTCGAAGGTCGAGTGGATCAAACAGGAGGGGCGCACCATTCCCGTGCCGGTATCC
GGCAGCGAAGAGATCATTCCAGCCCAGCTCGTGCTGCTGGCGATGGGCTTCCTCGGGCCAGAAGCGCAGCTCTTGCAGTC
GCTTGGCGTCGAGCAGGACAGCCGCAGCAACATCAAGGCTGACGAGAAAAGCTACCGTACCAGCGTTGACAAGGTTTTCG
CCGCAGGCGATGCCCGCCGTGGTCAGAGCCTCGTGGTGTGGGCGATCAACGAAGGGCGCGCCGCTGCCAGAGAGTGCGAC
CGCTTCCTGATGGGTTGCACCAGCCTGCCGTGA

Upstream 100 bases:

>100_bases
TGAAGCCGTCATGGCCGCCTTCGAAAAGAACGTGCATGATCCTTCCCGTGCCTCGGGGAACTGAAATTGGGCGAGTCGCC
TGAACAACGTATTACGACCT

Downstream 100 bases:

>100_bases
ACGAATCGGCAACCGACTGTGTTTGAAGAGGACGAAAAAGGCTGAATAAATCCGGATACGTTGTATGGAACAGCAAAAAC
TGAGGGAGCTGCTCGAAACC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGKLKGFMEYRRALPVDREPLERIKDWNEFHEEMSAEQLSDQGARCMDCGTPFCHSGFMLNGMTAGCPIHNLIPEWNDHV
YRGFWRDAWERLMKTNNFPEFTGRVCPAPCEGSCVLGIIQPPVTIKNIEYSIIEHAFAEGWVEPKQIAVRTGKKVAIVGS
GPSGLACADQLNKAGHTVTVFERDDRVGGLLMYGIPNMKLDKRLVVQRRVDLMKEEGVSFVTGTEVGVNYPVDKLLSEYD
AVVLCIGATNPRDLNADGRNLDGIHFAMEFLRASTKAVLDGTEPVLSAKGKDVVVIGGGDTGTDCVATSLRQGCKSVIQL
EIMPKPADFRQEDNPWPEWPKVFKVDYGQEEAAAVQGGDPRRYLMMTKKFIGENGRLSAVEVSKVEWIKQEGRTIPVPVS
GSEEIIPAQLVLLAMGFLGPEAQLLQSLGVEQDSRSNIKADEKSYRTSVDKVFAAGDARRGQSLVVWAINEGRAAARECD
RFLMGCTSLP

Sequences:

>Translated_490_residues
MGKLKGFMEYRRALPVDREPLERIKDWNEFHEEMSAEQLSDQGARCMDCGTPFCHSGFMLNGMTAGCPIHNLIPEWNDHV
YRGFWRDAWERLMKTNNFPEFTGRVCPAPCEGSCVLGIIQPPVTIKNIEYSIIEHAFAEGWVEPKQIAVRTGKKVAIVGS
GPSGLACADQLNKAGHTVTVFERDDRVGGLLMYGIPNMKLDKRLVVQRRVDLMKEEGVSFVTGTEVGVNYPVDKLLSEYD
AVVLCIGATNPRDLNADGRNLDGIHFAMEFLRASTKAVLDGTEPVLSAKGKDVVVIGGGDTGTDCVATSLRQGCKSVIQL
EIMPKPADFRQEDNPWPEWPKVFKVDYGQEEAAAVQGGDPRRYLMMTKKFIGENGRLSAVEVSKVEWIKQEGRTIPVPVS
GSEEIIPAQLVLLAMGFLGPEAQLLQSLGVEQDSRSNIKADEKSYRTSVDKVFAAGDARRGQSLVVWAINEGRAAARECD
RFLMGCTSLP
>Mature_489_residues
GKLKGFMEYRRALPVDREPLERIKDWNEFHEEMSAEQLSDQGARCMDCGTPFCHSGFMLNGMTAGCPIHNLIPEWNDHVY
RGFWRDAWERLMKTNNFPEFTGRVCPAPCEGSCVLGIIQPPVTIKNIEYSIIEHAFAEGWVEPKQIAVRTGKKVAIVGSG
PSGLACADQLNKAGHTVTVFERDDRVGGLLMYGIPNMKLDKRLVVQRRVDLMKEEGVSFVTGTEVGVNYPVDKLLSEYDA
VVLCIGATNPRDLNADGRNLDGIHFAMEFLRASTKAVLDGTEPVLSAKGKDVVVIGGGDTGTDCVATSLRQGCKSVIQLE
IMPKPADFRQEDNPWPEWPKVFKVDYGQEEAAAVQGGDPRRYLMMTKKFIGENGRLSAVEVSKVEWIKQEGRTIPVPVSG
SEEIIPAQLVLLAMGFLGPEAQLLQSLGVEQDSRSNIKADEKSYRTSVDKVFAAGDARRGQSLVVWAINEGRAAARECDR
FLMGCTSLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=508, Percent_Identity=23.4251968503937, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=38.1742738589212, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1788811, Length=486, Percent_Identity=36.2139917695473, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=33.974358974359, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=25.1670378619154, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789243, Length=416, Percent_Identity=26.4423076923077, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=502, Percent_Identity=50.996015936255, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71984108, Length=492, Percent_Identity=24.390243902439, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=494, Percent_Identity=50.8097165991903, Blast_Score=509, Evalue=1e-145,
Organism=Drosophila melanogaster, GI28574881, Length=487, Percent_Identity=58.3162217659138, Blast_Score=577, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24665539, Length=487, Percent_Identity=58.3162217659138, Blast_Score=577, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24665547, Length=487, Percent_Identity=58.3162217659138, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24665543, Length=487, Percent_Identity=58.3162217659138, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24640763, Length=474, Percent_Identity=25.5274261603376, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18858217, Length=474, Percent_Identity=25.5274261603376, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54088; Mature: 53956

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLKGFMEYRRALPVDREPLERIKDWNEFHEEMSAEQLSDQGARCMDCGTPFCHSGFML
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHCCCEEE
NGMTAGCPIHNLIPEWNDHVYRGFWRDAWERLMKTNNFPEFTGRVCPAPCEGSCVLGIIQ
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIEYSIIEHAFAEGWVEPKQIAVRTGKKVAIVGSGPSGLACADQLNKAGHTVTV
CCEEEECCHHHHHHHHHHHCCCCHHHHHEECCCEEEEEECCCCCCHHHHHHHCCCCEEEE
FERDDRVGGLLMYGIPNMKLDKRLVVQRRVDLMKEEGVSFVTGTEVGVNYPVDKLLSEYD
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCC
AVVLCIGATNPRDLNADGRNLDGIHFAMEFLRASTKAVLDGTEPVLSAKGKDVVVIGGGD
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCC
TGTDCVATSLRQGCKSVIQLEIMPKPADFRQEDNPWPEWPKVFKVDYGQEEAAAVQGGDP
CCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCC
RRYLMMTKKFIGENGRLSAVEVSKVEWIKQEGRTIPVPVSGSEEIIPAQLVLLAMGFLGP
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCC
EAQLLQSLGVEQDSRSNIKADEKSYRTSVDKVFAAGDARRGQSLVVWAINEGRAAARECD
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHH
RFLMGCTSLP
HHHHHHCCCC
>Mature Secondary Structure 
GKLKGFMEYRRALPVDREPLERIKDWNEFHEEMSAEQLSDQGARCMDCGTPFCHSGFML
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHCCCEEE
NGMTAGCPIHNLIPEWNDHVYRGFWRDAWERLMKTNNFPEFTGRVCPAPCEGSCVLGIIQ
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIEYSIIEHAFAEGWVEPKQIAVRTGKKVAIVGSGPSGLACADQLNKAGHTVTV
CCEEEECCHHHHHHHHHHHCCCCHHHHHEECCCEEEEEECCCCCCHHHHHHHCCCCEEEE
FERDDRVGGLLMYGIPNMKLDKRLVVQRRVDLMKEEGVSFVTGTEVGVNYPVDKLLSEYD
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCC
AVVLCIGATNPRDLNADGRNLDGIHFAMEFLRASTKAVLDGTEPVLSAKGKDVVVIGGGD
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCC
TGTDCVATSLRQGCKSVIQLEIMPKPADFRQEDNPWPEWPKVFKVDYGQEEAAAVQGGDP
CCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCC
RRYLMMTKKFIGENGRLSAVEVSKVEWIKQEGRTIPVPVSGSEEIIPAQLVLLAMGFLGP
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCC
EAQLLQSLGVEQDSRSNIKADEKSYRTSVDKVFAAGDARRGQSLVVWAINEGRAAARECD
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHH
RFLMGCTSLP
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]