Click here to switch to the map view.

The map label for this gene is carA

Identifier: 21672907

GI number: 21672907

Start: 66066

End: 67166

Strand: Direct

Name: carA

Synonym: CT0066

Alternate gene names: 21672907

Gene position: 66066-67166 (Clockwise)

Preceding gene: 21672906

Following gene: 21672908

Centisome position: 3.07

GC content: 58.13

Gene sequence:

>1101_bases
ATGCAGGAAATACCCGCAATTCTGGTTCTTGAAAACGGCTCGGTCTATCGCGGCACCGCCTTTGGCCATGCTGGTGAAGC
GACAGGCGAAGTGGTTTTCAACACCTCGCTGACCGGCTATCAGGAAATTCTGACCGATCCGTCCTACACCGGACAGATGG
TGGTTATGACCTACCCGCTCATTGGCAACTATGGCATTACGCCGAACGATAACGAGTCGAAGAAAATCTGGGCTTCGGCG
CTGATTGTGCGTGAAGCCTCCAACGTTTACAGCAACTACGAATCGACCCGCAGCCTCGACGCGACACTCAAAGAGGCTGG
CGTGATGGGGCTTGCCGGCATCGATACCCGAAAGCTGGTGAGAGAGATTCGCCAGAAAGGCGCGATGCGGGCGGTGATTT
CGACCTTGTGCGATGACGTCGCGGCGCTCAAGGCGCAGGCGGCGGCCATTCCCGAGATGACCGGCCTCGACCTGGTGCAG
CGCGTCACCACCGGAGAGCGCTACACGGTGGACAATCCCGACGCCAAATATCACGTCGTCGCTTTCGACTATGGCATTAA
GACCAACATCATCCGCCAGCTCAACGCCGAGGGGTGCAAGGTCACGGTGGTCAACGCCAAAACCACCGCTGACGAGGTGC
TCGCCATGAATCCCGATGGCATCTTTCTCTCGAACGGCCCTGGCGATCCTTTTGCGGTCACTTACGCCATCGACACCATC
CGGGAGCTGGCCGCACGGAACAGCACGTTGCCGATCTTCGGCATCTGCCTTGGTCACCAGTTGCTTTCGCTCGCGTTCGG
AGCGAAGACCTACAAGCTCAAGTTTGGCCATCACGGCGCGAATCATCCGGTAAAGAACCTCTTGAGCAACACGATCGAGA
TCACCTCGCAGAACCACGGCTTTGCCGTCGAAATGGAGAGTCTGCCGGGTGAGCTGGAGCTGACTCACAAGAATCTCTAC
GACATGACCGTCGAGGGTATCCGCCACCGCGAGCTGCCCTGCTTCTCGGTGCAGTACCACCCCGAAGCCGCTCCCGGCCC
GCACGACTCGCACTACCTCTTCAAAGAGTTCACCGAACTCATGGAGAGGTTGAAAAACTGA

Upstream 100 bases:

>100_bases
TGCCGACAACGTCAAGATCAAGTTCGAACGTTCGGCGATTGCCAACATAGAAAAACAGGAAACCGGCGACAAGCTCGCCT
CGAAGGAGTAAGAAGCATCG

Downstream 100 bases:

>100_bases
CCCGCTCCGGTGCTGGGGTTCCGAAATGAGCGCCAGCGCCTCTTCCGGCAGCAAGAGCCGACAACACAGGGTTTTACATC
AGCGGCGCGGTTGGCATCGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQEIPAILVLENGSVYRGTAFGHAGEATGEVVFNTSLTGYQEILTDPSYTGQMVVMTYPLIGNYGITPNDNESKKIWASA
LIVREASNVYSNYESTRSLDATLKEAGVMGLAGIDTRKLVREIRQKGAMRAVISTLCDDVAALKAQAAAIPEMTGLDLVQ
RVTTGERYTVDNPDAKYHVVAFDYGIKTNIIRQLNAEGCKVTVVNAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTI
RELAARNSTLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIEITSQNHGFAVEMESLPGELELTHKNLY
DMTVEGIRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMERLKN

Sequences:

>Translated_366_residues
MQEIPAILVLENGSVYRGTAFGHAGEATGEVVFNTSLTGYQEILTDPSYTGQMVVMTYPLIGNYGITPNDNESKKIWASA
LIVREASNVYSNYESTRSLDATLKEAGVMGLAGIDTRKLVREIRQKGAMRAVISTLCDDVAALKAQAAAIPEMTGLDLVQ
RVTTGERYTVDNPDAKYHVVAFDYGIKTNIIRQLNAEGCKVTVVNAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTI
RELAARNSTLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIEITSQNHGFAVEMESLPGELELTHKNLY
DMTVEGIRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMERLKN
>Mature_366_residues
MQEIPAILVLENGSVYRGTAFGHAGEATGEVVFNTSLTGYQEILTDPSYTGQMVVMTYPLIGNYGITPNDNESKKIWASA
LIVREASNVYSNYESTRSLDATLKEAGVMGLAGIDTRKLVREIRQKGAMRAVISTLCDDVAALKAQAAAIPEMTGLDLVQ
RVTTGERYTVDNPDAKYHVVAFDYGIKTNIIRQLNAEGCKVTVVNAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTI
RELAARNSTLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIEITSQNHGFAVEMESLPGELELTHKNLY
DMTVEGIRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMERLKN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=365, Percent_Identity=42.1917808219178, Blast_Score=271, Evalue=9e-73,
Organism=Homo sapiens, GI21361331, Length=373, Percent_Identity=37.5335120643432, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI169790915, Length=373, Percent_Identity=37.5335120643432, Blast_Score=228, Evalue=5e-60,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=50.2659574468085, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1789760, Length=146, Percent_Identity=34.2465753424658, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=373, Percent_Identity=41.0187667560322, Blast_Score=266, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=40.053050397878, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=400, Percent_Identity=37, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322638, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45555749, Length=383, Percent_Identity=36.8146214099217, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642586, Length=383, Percent_Identity=36.8146214099217, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_CHLTE (Q8KGA2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660972.1
- ProteinModelPortal:   Q8KGA2
- SMR:   Q8KGA2
- GeneID:   1006486
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0066
- NMPDR:   fig|194439.1.peg.66
- TIGR:   CT0066
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   CTEP194439:CT_0066-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40111; Mature: 40111

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 256-256 ACT_SITE 340-340 ACT_SITE 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIPAILVLENGSVYRGTAFGHAGEATGEVVFNTSLTGYQEILTDPSYTGQMVVMTYPL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESKKIWASALIVREASNVYSNYESTRSLDATLKEAGVMGLAGIDTRKLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
REIRQKGAMRAVISTLCDDVAALKAQAAAIPEMTGLDLVQRVTTGERYTVDNPDAKYHVV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEE
AFDYGIKTNIIRQLNAEGCKVTVVNAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTI
EEECCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCCEEEEHHHHHH
RELAARNSTLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIEITSQNHG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCC
FAVEMESLPGELELTHKNLYDMTVEGIRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTEL
EEEECCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
MERLKN
HHHHCC
>Mature Secondary Structure
MQEIPAILVLENGSVYRGTAFGHAGEATGEVVFNTSLTGYQEILTDPSYTGQMVVMTYPL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESKKIWASALIVREASNVYSNYESTRSLDATLKEAGVMGLAGIDTRKLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
REIRQKGAMRAVISTLCDDVAALKAQAAAIPEMTGLDLVQRVTTGERYTVDNPDAKYHVV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEE
AFDYGIKTNIIRQLNAEGCKVTVVNAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTI
EEECCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCCEEEEHHHHHH
RELAARNSTLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIEITSQNHG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCC
FAVEMESLPGELELTHKNLYDMTVEGIRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTEL
EEEECCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
MERLKN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901