Click here to switch to the map view.

The map label for this gene is bioB

Identifier: 21672893

GI number: 21672893

Start: 50109

End: 51110

Strand: Reverse

Name: bioB

Synonym: CT0052

Alternate gene names: 21672893

Gene position: 51110-50109 (Counterclockwise)

Preceding gene: 21672894

Following gene: 21672892

Centisome position: 2.37

GC content: 63.57

Gene sequence:

>1002_bases
ATGAAGTCACGACTGCATCCCGACATTGAAAAGGCCTACGCCGTGCTCGACACCGGCGAGCCGCTCTCGCTCGAACTCGC
TTCGGCGCTGGGGCGGCTTCCGGACTCCGAGGTGCTCGACCTCGTATCGCTGGCCAACCGGGTCAAGGCCCGCCACGCCG
CGAACCACGGCGCGATTCACGCCTGCTCGATCATGAACGCCAAATCGGGCGTATGCGGTGAGAACTGCCGCTTCTGCGCC
CAGTCGAAGCACAACAGCGCCGAGGTCGATGTCTATGAGCTGGTCGATGAGAACAAGGTGCTCGAACAGGCGCGGAGCGC
GTGGGAGCAGGGCATCGGCCACTTCGGCATCGTGACCAGCGGCTATGGCTACCTGAAGGTGACGCCTGAGTTCGAGCGAA
TTCTTGGCATGATCGACCGGCTGCATCGCGAACTGCCCGGTCTGCATGTCTGCGCTTCACTCGGCGTGCTCGGCGACGCG
CCCGCCGCCGAACTGGCCCGCCACGGCATCGCACACTACAACATCAACATCCAGGTCGATCCGGCCCGCTACGGCGAACT
GATCGCCGACACGCACGCGGTTAACGAACGGATTGGAACAATCCGGCGGCTTCGCTCAAACGGCATCGGCGTCTGTTGCG
GCGGCATCCTCGGCGTCGGCGAAACGATGCAGGAGCGCATCGGCATGATCTTCGCGCTGCGAGACCTCGACGTAACGGTC
ATTCCGCTCAACGTGCTGGTACCCATCGACGGCACGCCGCTCGAAGGAGCCGCACCGGTCTCGGTGCCGGAAATCGCCAA
AACCTTCGCCATCTGCCGCCTCGCGCACCCGACAAAGATCATCAAGTTCGCCGCCGGACGCGAAACCGTGATGAAGGATT
TTCAGGGTCTGCTCATGCTGGCGGGAGCGGATGGCTTCCTCACCGGCGGTTACCTGACGACGCGGGGACGTGACATTTCC
ACCGACCGGCAGCTCGCCCGGCAACTCTCGAAGTTCTCATGA

Upstream 100 bases:

>100_bases
CGCAGGGCCACCTCCTCTTGCGCACCGTCGATGGCGCTCTGCTGTCTGTCACTTCGGGCGAGGCTCACCTGCTTCCCGTC
AACCACGAACCGAGATCCGC

Downstream 100 bases:

>100_bases
GCGGCGGATTTACGAATGCGGGCCAGGTCGAGCCGCCGATTGTGGCCGACATCGCGGTGGAGCTTGCGAAGCTCAAGGCG
AAGCAGCGCTTCCGCTCGAT

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKSRLHPDIEKAYAVLDTGEPLSLELASALGRLPDSEVLDLVSLANRVKARHAANHGAIHACSIMNAKSGVCGENCRFCA
QSKHNSAEVDVYELVDENKVLEQARSAWEQGIGHFGIVTSGYGYLKVTPEFERILGMIDRLHRELPGLHVCASLGVLGDA
PAAELARHGIAHYNINIQVDPARYGELIADTHAVNERIGTIRRLRSNGIGVCCGGILGVGETMQERIGMIFALRDLDVTV
IPLNVLVPIDGTPLEGAAPVSVPEIAKTFAICRLAHPTKIIKFAAGRETVMKDFQGLLMLAGADGFLTGGYLTTRGRDIS
TDRQLARQLSKFS

Sequences:

>Translated_333_residues
MKSRLHPDIEKAYAVLDTGEPLSLELASALGRLPDSEVLDLVSLANRVKARHAANHGAIHACSIMNAKSGVCGENCRFCA
QSKHNSAEVDVYELVDENKVLEQARSAWEQGIGHFGIVTSGYGYLKVTPEFERILGMIDRLHRELPGLHVCASLGVLGDA
PAAELARHGIAHYNINIQVDPARYGELIADTHAVNERIGTIRRLRSNGIGVCCGGILGVGETMQERIGMIFALRDLDVTV
IPLNVLVPIDGTPLEGAAPVSVPEIAKTFAICRLAHPTKIIKFAAGRETVMKDFQGLLMLAGADGFLTGGYLTTRGRDIS
TDRQLARQLSKFS
>Mature_333_residues
MKSRLHPDIEKAYAVLDTGEPLSLELASALGRLPDSEVLDLVSLANRVKARHAANHGAIHACSIMNAKSGVCGENCRFCA
QSKHNSAEVDVYELVDENKVLEQARSAWEQGIGHFGIVTSGYGYLKVTPEFERILGMIDRLHRELPGLHVCASLGVLGDA
PAAELARHGIAHYNINIQVDPARYGELIADTHAVNERIGTIRRLRSNGIGVCCGGILGVGETMQERIGMIFALRDLDVTV
IPLNVLVPIDGTPLEGAAPVSVPEIAKTFAICRLAHPTKIIKFAAGRETVMKDFQGLLMLAGADGFLTGGYLTTRGRDIS
TDRQLARQLSKFS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=31.0457516339869, Blast_Score=161, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6321725, Length=307, Percent_Identity=30.9446254071661, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CHLTE (Q8KGB6)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660958.1
- HSSP:   P12996
- ProteinModelPortal:   Q8KGB6
- SMR:   Q8KGB6
- GeneID:   1006470
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0052
- NMPDR:   fig|194439.1.peg.52
- TIGR:   CT0052
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK637131
- BioCyc:   CTEP194439:CT_0052-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35938; Mature: 35938

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRLHPDIEKAYAVLDTGEPLSLELASALGRLPDSEVLDLVSLANRVKARHAANHGAIH
CCCCCCCCHHHHEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
ACSIMNAKSGVCGENCRFCAQSKHNSAEVDVYELVDENKVLEQARSAWEQGIGHFGIVTS
EEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GYGYLKVTPEFERILGMIDRLHRELPGLHVCASLGVLGDAPAAELARHGIAHYNINIQVD
CCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHCCCEEEEEEEEEC
PARYGELIADTHAVNERIGTIRRLRSNGIGVCCGGILGVGETMQERIGMIFALRDLDVTV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHCEEEEEECCCEEE
IPLNVLVPIDGTPLEGAAPVSVPEIAKTFAICRLAHPTKIIKFAAGRETVMKDFQGLLML
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCEEEE
AGADGFLTGGYLTTRGRDISTDRQLARQLSKFS
ECCCCCEECCEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKSRLHPDIEKAYAVLDTGEPLSLELASALGRLPDSEVLDLVSLANRVKARHAANHGAIH
CCCCCCCCHHHHEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
ACSIMNAKSGVCGENCRFCAQSKHNSAEVDVYELVDENKVLEQARSAWEQGIGHFGIVTS
EEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GYGYLKVTPEFERILGMIDRLHRELPGLHVCASLGVLGDAPAAELARHGIAHYNINIQVD
CCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHCCCEEEEEEEEEC
PARYGELIADTHAVNERIGTIRRLRSNGIGVCCGGILGVGETMQERIGMIFALRDLDVTV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHCEEEEEECCCEEE
IPLNVLVPIDGTPLEGAAPVSVPEIAKTFAICRLAHPTKIIKFAAGRETVMKDFQGLLML
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCEEEE
AGADGFLTGGYLTTRGRDISTDRQLARQLSKFS
ECCCCCEECCEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901