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The map label for this gene is mak [H]

Identifier: 21672849

GI number: 21672849

Start: 6640

End: 7563

Strand: Direct

Name: mak [H]

Synonym: CT0008

Alternate gene names: 21672849

Gene position: 6640-7563 (Clockwise)

Preceding gene: 21672848

Following gene: 21672850

Centisome position: 0.31

GC content: 66.88

Gene sequence:

>924_bases
ATGAATCGCTGGGGAATCGATCTCGGCGGCACCAAGATCGAGGGGGTGATCCTCGACAGCGAGTTGCGCCCTCTCATTCG
CCATCGCATTCCGACCGGGCAAGAGCAGGGGTACGGCCATATTCTCATGCAGATCAAAAGCCTCGTCGGGACGATGGCCG
AGAAGTCGGGACTCGGCCTGCCGGAGAAGATAGGCATCGGCACGCCCGGGCGCGCCGATGGTAGCGACGGCGTCATCAGC
AACTCGAACACGATCTGCCTGAACGGAATGCCGCTCTTGCGCGATCTGCAAGAGGCGCTCCGGCTGGAGGTGGTGATCGA
CAACGACGCGAACTGCTTTGCGCTCGCCGAGTCGATGCTCGGCGCGGGGCGCGACGAGATGGCGCGGCCAGGCGCGACAG
CTTTCGGTATCATTCTCGGCACCGGCGTGGGCGGGGGCATCGTCCGCGATGGCCGGATCATCCGGGGCGCGCACGGCATC
GCCGGAGAGTGGGGGCACAACCCGCTGCCGGGCGAGCACGCGGCGTGCTACTGCGGTCGGCGCGGCTGCGTCGAGACGGT
CGTCTCCGGCCCGGCACTCGAGCGCCACTACGCGGCCCTCAGCGGGCGAAAGGCGTCGCTGCAAGAGATCGCCGCCTCGA
CGGGGCGCGACCGCTTCGCACGCCAGACCATCGAGCGGCTCGTTTCGAAATTCGGCGTGGCGCTGGCGACGGTTATCAAC
ATTCTCGATCCCGACCTGGTCATCATCGGCGGCGGCGTGGGCAACATCCGCCAGCTCTACTCCCCGGAGGCGCGGCAGGC
GATTGCCGCCAACGTTTTCAACCGCTCGTTCGACATTCCGCTCCTGCCGCCGATGCTTGGCGACAGCGCGGGCGTGTTCG
GCGCAGCACTGCTCTCGGGGCCGCCACTCATTGCACAGTATTGA

Upstream 100 bases:

>100_bases
TCGTGATTGGTCTTGCCAGCGCGGCGGTGATCTACACGGCGTTCGCCTGGATCGTCAAGAACGTGGTGCACCGGCGAGTG
ATGCAGAGGGAGGGCTCGGA

Downstream 100 bases:

>100_bases
TTCACCGCGACTCCCCTGCGGAGCGCTTATTGCACACTCCATTGGAACAACAGGAAATCCGCTACAGAAAAGGCGACATC
ATCGAACTCACCATCACTGA

Product: Rok family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNRWGIDLGGTKIEGVILDSELRPLIRHRIPTGQEQGYGHILMQIKSLVGTMAEKSGLGLPEKIGIGTPGRADGSDGVIS
NSNTICLNGMPLLRDLQEALRLEVVIDNDANCFALAESMLGAGRDEMARPGATAFGIILGTGVGGGIVRDGRIIRGAHGI
AGEWGHNPLPGEHAACYCGRRGCVETVVSGPALERHYAALSGRKASLQEIAASTGRDRFARQTIERLVSKFGVALATVIN
ILDPDLVIIGGGVGNIRQLYSPEARQAIAANVFNRSFDIPLLPPMLGDSAGVFGAALLSGPPLIAQY

Sequences:

>Translated_307_residues
MNRWGIDLGGTKIEGVILDSELRPLIRHRIPTGQEQGYGHILMQIKSLVGTMAEKSGLGLPEKIGIGTPGRADGSDGVIS
NSNTICLNGMPLLRDLQEALRLEVVIDNDANCFALAESMLGAGRDEMARPGATAFGIILGTGVGGGIVRDGRIIRGAHGI
AGEWGHNPLPGEHAACYCGRRGCVETVVSGPALERHYAALSGRKASLQEIAASTGRDRFARQTIERLVSKFGVALATVIN
ILDPDLVIIGGGVGNIRQLYSPEARQAIAANVFNRSFDIPLLPPMLGDSAGVFGAALLSGPPLIAQY
>Mature_307_residues
MNRWGIDLGGTKIEGVILDSELRPLIRHRIPTGQEQGYGHILMQIKSLVGTMAEKSGLGLPEKIGIGTPGRADGSDGVIS
NSNTICLNGMPLLRDLQEALRLEVVIDNDANCFALAESMLGAGRDEMARPGATAFGIILGTGVGGGIVRDGRIIRGAHGI
AGEWGHNPLPGEHAACYCGRRGCVETVVSGPALERHYAALSGRKASLQEIAASTGRDRFARQTIERLVSKFGVALATVIN
ILDPDLVIIGGGVGNIRQLYSPEARQAIAANVFNRSFDIPLLPPMLGDSAGVFGAALLSGPPLIAQY

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=268, Percent_Identity=29.1044776119403, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI190014632, Length=272, Percent_Identity=28.6764705882353, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4885285, Length=272, Percent_Identity=28.6764705882353, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI298566315, Length=272, Percent_Identity=28.6764705882353, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081733, Length=306, Percent_Identity=40.1960784313725, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787363, Length=310, Percent_Identity=33.8709677419355, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790522, Length=172, Percent_Identity=33.7209302325581, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786891, Length=298, Percent_Identity=24.496644295302, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787878, Length=210, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32246; Mature: 32246

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRWGIDLGGTKIEGVILDSELRPLIRHRIPTGQEQGYGHILMQIKSLVGTMAEKSGLGL
CCCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PEKIGIGTPGRADGSDGVISNSNTICLNGMPLLRDLQEALRLEVVIDNDANCFALAESML
CHHCCCCCCCCCCCCCCEECCCCEEEECCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHH
GAGRDEMARPGATAFGIILGTGVGGGIVRDGRIIRGAHGIAGEWGHNPLPGEHAACYCGR
CCCCHHHCCCCCCEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHCC
RGCVETVVSGPALERHYAALSGRKASLQEIAASTGRDRFARQTIERLVSKFGVALATVIN
CHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILDPDLVIIGGGVGNIRQLYSPEARQAIAANVFNRSFDIPLLPPMLGDSAGVFGAALLSG
HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCC
PPLIAQY
CCCCCCC
>Mature Secondary Structure
MNRWGIDLGGTKIEGVILDSELRPLIRHRIPTGQEQGYGHILMQIKSLVGTMAEKSGLGL
CCCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PEKIGIGTPGRADGSDGVISNSNTICLNGMPLLRDLQEALRLEVVIDNDANCFALAESML
CHHCCCCCCCCCCCCCCEECCCCEEEECCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHH
GAGRDEMARPGATAFGIILGTGVGGGIVRDGRIIRGAHGIAGEWGHNPLPGEHAACYCGR
CCCCHHHCCCCCCEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHCC
RGCVETVVSGPALERHYAALSGRKASLQEIAASTGRDRFARQTIERLVSKFGVALATVIN
CHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILDPDLVIIGGGVGNIRQLYSPEARQAIAANVFNRSFDIPLLPPMLGDSAGVFGAALLSG
HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCC
PPLIAQY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]