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The map label for this gene is prfC

Identifier: 21672788

GI number: 21672788

Start: 577410

End: 578999

Strand: Reverse

Name: prfC

Synonym: BUsg523

Alternate gene names: 21672788

Gene position: 578999-577410 (Counterclockwise)

Preceding gene: 21672789

Following gene: 21672787

Centisome position: 90.26

GC content: 27.48

Gene sequence:

>1590_bases
ATGTTTAATATTAATCATCAATTGGAGTTAAGTAAAAGGCGTACCTTTGCTATTATTTCTCATCCTGACGCCGGTAAAAC
AACTGTGACAGAAAAAATGTTATTACTTGGAAAAGTAATTCGTACTTCTGGAACTATTAAAGCCAGAGGAAACGGAAAAT
ATGCTAAATCAGACTGGATGAACATTGAAAAAAAGCGTGGAATTTCTATTACTACCTCAGTTATGCAATTTACATATAAG
AATACTTTAATGAATTTGCTAGATACCCCCGGTCATGAAGATTTTTCAGAAGATACATATCGAATTCTTACTGCAGTTGA
TTGTTGTTTATTAATTATCGATGCTGCTAAAGGAATAGAAGATAGAACAAAAAAATTAATTAATGTTAGTCGTTTACATA
ATACTCCTGTAATTACTTTTATTAATAAATTAGATCGTGATAGTCGTGAAGCAATAGAGATTCTAGATGAAATAGAAAAA
GAATTAAGTTTAGACTGCATTCCAATAACTTGGCCTATCAGTTGTGGAAAAAACTTTAAAGGTATTTGTCATATCCATGA
TAAAATAGTTAACTTATATCAAAAAAATATCGTTAAAAAAATTAATTTTAACTCTTTTTCTTCTTTTTTAAATGAACATT
CCTTAAAAGAGTATCTTGGTGCAGATCTATATACACAATTGCATGAAGAATTAAAATTAGCTGCCTATGTGTATCCAAAA
TTTAGTAAAAAAAAATTTTTAAAAGGTGATATTACCCCTGTTCTTTTTGGTAGTGCGTTAAACAATTTCGGTATTGATCA
TATACTAGAAAGTTTAATAAAATGGGCTCCTTCTCCTATTTATCGTGTTACTAAAACACGAAAAGTAGAACCTGAAGAAA
AAAAATTTACAGGTTTTGTATTTAAAATTCAGGCTAATATGGATTTAAAACATCGTGATCGTATAGCTTTTATGAGAATT
GTTTCTGGAAAATATAAAAAAGGTATAAAATTAAGACATGTACGCATGAAAAAAGATATTATTGTTTCTGATGCATTTTC
TTTTTTAGCTGGAGATAGATTTTCTATAGAACATGCATATCCTGGAGATGTAATAGGAATACATAATCATGGAACAATAA
AAATTGGCGATACCTTTACAGAAGGAGAAGAAATCAAATTTGTTGGGATTCCAAGCTTTGCTCCAGAAGTATTTCGTCGT
ATTTTTTTAAAAAATCCATTACAACAAAAGAAATTAAAAAAGGGTTTATCACAATTGTCAGAAGAAGGTGCAATACAAGT
TTTTCGTCCTATGATTAATAATAGTTTAATTTTAGGTGCTATTGGAATACTGCAATTTGATGTTGTTATTGAACGTTTAA
AGATTGAATACAAAATAGATGCAATTTATGAAAGAGTAAATATCTCTCTTGCACGTTGGATTAGATCGAAAAATAAAAAA
ATTATTTCTGATTTTATAAATAAAAATAAATCTTATTTAGCGTTAGATATTTCTAATCAACTAATATATTTAGCGCCAAA
TAAAGCTAATTTAGCTGTAATAAAAAATATTTATAATAATATTTTTTTTGAAAAAACAAGAGAACAATAA

Upstream 100 bases:

>100_bases
AAAATATTTGAATATTTGTTTTTAACAAATAATTTTTATTTTTACTACTTAAAATTTTGATTAAAATTTTAATAATGAAA
AATAATTTAAATGAGTATAT

Downstream 100 bases:

>100_bases
ATATATTTTATTAATTTTAGGTATTTATAATGAAACGTGTTTTTTTGATGATTTTAGATTCTTTAGGAATAGGTTCAAGT
GTTGATGCTTGTAAATTTAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MFNINHQLELSKRRTFAIISHPDAGKTTVTEKMLLLGKVIRTSGTIKARGNGKYAKSDWMNIEKKRGISITTSVMQFTYK
NTLMNLLDTPGHEDFSEDTYRILTAVDCCLLIIDAAKGIEDRTKKLINVSRLHNTPVITFINKLDRDSREAIEILDEIEK
ELSLDCIPITWPISCGKNFKGICHIHDKIVNLYQKNIVKKINFNSFSSFLNEHSLKEYLGADLYTQLHEELKLAAYVYPK
FSKKKFLKGDITPVLFGSALNNFGIDHILESLIKWAPSPIYRVTKTRKVEPEEKKFTGFVFKIQANMDLKHRDRIAFMRI
VSGKYKKGIKLRHVRMKKDIIVSDAFSFLAGDRFSIEHAYPGDVIGIHNHGTIKIGDTFTEGEEIKFVGIPSFAPEVFRR
IFLKNPLQQKKLKKGLSQLSEEGAIQVFRPMINNSLILGAIGILQFDVVIERLKIEYKIDAIYERVNISLARWIRSKNKK
IISDFINKNKSYLALDISNQLIYLAPNKANLAVIKNIYNNIFFEKTREQ

Sequences:

>Translated_529_residues
MFNINHQLELSKRRTFAIISHPDAGKTTVTEKMLLLGKVIRTSGTIKARGNGKYAKSDWMNIEKKRGISITTSVMQFTYK
NTLMNLLDTPGHEDFSEDTYRILTAVDCCLLIIDAAKGIEDRTKKLINVSRLHNTPVITFINKLDRDSREAIEILDEIEK
ELSLDCIPITWPISCGKNFKGICHIHDKIVNLYQKNIVKKINFNSFSSFLNEHSLKEYLGADLYTQLHEELKLAAYVYPK
FSKKKFLKGDITPVLFGSALNNFGIDHILESLIKWAPSPIYRVTKTRKVEPEEKKFTGFVFKIQANMDLKHRDRIAFMRI
VSGKYKKGIKLRHVRMKKDIIVSDAFSFLAGDRFSIEHAYPGDVIGIHNHGTIKIGDTFTEGEEIKFVGIPSFAPEVFRR
IFLKNPLQQKKLKKGLSQLSEEGAIQVFRPMINNSLILGAIGILQFDVVIERLKIEYKIDAIYERVNISLARWIRSKNKK
IISDFINKNKSYLALDISNQLIYLAPNKANLAVIKNIYNNIFFEKTREQ
>Mature_529_residues
MFNINHQLELSKRRTFAIISHPDAGKTTVTEKMLLLGKVIRTSGTIKARGNGKYAKSDWMNIEKKRGISITTSVMQFTYK
NTLMNLLDTPGHEDFSEDTYRILTAVDCCLLIIDAAKGIEDRTKKLINVSRLHNTPVITFINKLDRDSREAIEILDEIEK
ELSLDCIPITWPISCGKNFKGICHIHDKIVNLYQKNIVKKINFNSFSSFLNEHSLKEYLGADLYTQLHEELKLAAYVYPK
FSKKKFLKGDITPVLFGSALNNFGIDHILESLIKWAPSPIYRVTKTRKVEPEEKKFTGFVFKIQANMDLKHRDRIAFMRI
VSGKYKKGIKLRHVRMKKDIIVSDAFSFLAGDRFSIEHAYPGDVIGIHNHGTIKIGDTFTEGEEIKFVGIPSFAPEVFRR
IFLKNPLQQKKLKKGLSQLSEEGAIQVFRPMINNSLILGAIGILQFDVVIERLKIEYKIDAIYERVNISLARWIRSKNKK
IISDFINKNKSYLALDISNQLIYLAPNKANLAVIKNIYNNIFFEKTREQ

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=526, Percent_Identity=24.9049429657795, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI19923640, Length=500, Percent_Identity=25, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI25306283, Length=345, Percent_Identity=28.1159420289855, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI25306287, Length=293, Percent_Identity=29.3515358361775, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157426893, Length=202, Percent_Identity=31.1881188118812, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=31.3868613138686, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=60.8445297504799, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=518, Percent_Identity=22.7799227799228, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI48994988, Length=193, Percent_Identity=29.5336787564767, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=28.9617486338798, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=26.2295081967213, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17556745, Length=205, Percent_Identity=30.7317073170732, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=31.3432835820896, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=32.3308270676692, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=32.3308270676692, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=487, Percent_Identity=24.8459958932238, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322359, Length=546, Percent_Identity=24.5421245421245, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=29.1139240506329, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=28.1879194630872, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=28.1879194630872, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221458488, Length=504, Percent_Identity=25.7936507936508, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=23.3193277310924, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=143, Percent_Identity=31.4685314685315, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_BUCAP (Q8K935)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660855.1
- ProteinModelPortal:   Q8K935
- SMR:   Q8K935
- EnsemblBacteria:   EBBUCT00000000375
- GeneID:   1005652
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg523
- GeneTree:   EBGT00050000007783
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   BAPH198804:BUSG523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 60739; Mature: 60739

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNINHQLELSKRRTFAIISHPDAGKTTVTEKMLLLGKVIRTSGTIKARGNGKYAKSDWM
CCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHC
NIEKKRGISITTSVMQFTYKNTLMNLLDTPGHEDFSEDTYRILTAVDCCLLIIDAAKGIE
CCHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTKKLINVSRLHNTPVITFINKLDRDSREAIEILDEIEKELSLDCIPITWPISCGKNFK
HHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GICHIHDKIVNLYQKNIVKKINFNSFSSFLNEHSLKEYLGADLYTQLHEELKLAAYVYPK
CEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEECCC
FSKKKFLKGDITPVLFGSALNNFGIDHILESLIKWAPSPIYRVTKTRKVEPEEKKFTGFV
CCCCHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEE
FKIQANMDLKHRDRIAFMRIVSGKYKKGIKLRHVRMKKDIIVSDAFSFLAGDRFSIEHAY
EEEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCC
PGDVIGIHNHGTIKIGDTFTEGEEIKFVGIPSFAPEVFRRIFLKNPLQQKKLKKGLSQLS
CCCEEEECCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EEGAIQVFRPMINNSLILGAIGILQFDVVIERLKIEYKIDAIYERVNISLARWIRSKNKK
HCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCHH
IISDFINKNKSYLALDISNQLIYLAPNKANLAVIKNIYNNIFFEKTREQ
HHHHHHCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFNINHQLELSKRRTFAIISHPDAGKTTVTEKMLLLGKVIRTSGTIKARGNGKYAKSDWM
CCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHC
NIEKKRGISITTSVMQFTYKNTLMNLLDTPGHEDFSEDTYRILTAVDCCLLIIDAAKGIE
CCHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTKKLINVSRLHNTPVITFINKLDRDSREAIEILDEIEKELSLDCIPITWPISCGKNFK
HHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GICHIHDKIVNLYQKNIVKKINFNSFSSFLNEHSLKEYLGADLYTQLHEELKLAAYVYPK
CEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEECCC
FSKKKFLKGDITPVLFGSALNNFGIDHILESLIKWAPSPIYRVTKTRKVEPEEKKFTGFV
CCCCHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEE
FKIQANMDLKHRDRIAFMRIVSGKYKKGIKLRHVRMKKDIIVSDAFSFLAGDRFSIEHAY
EEEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCC
PGDVIGIHNHGTIKIGDTFTEGEEIKFVGIPSFAPEVFRRIFLKNPLQQKKLKKGLSQLS
CCCEEEECCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EEGAIQVFRPMINNSLILGAIGILQFDVVIERLKIEYKIDAIYERVNISLARWIRSKNKK
HCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCHH
IISDFINKNKSYLALDISNQLIYLAPNKANLAVIKNIYNNIFFEKTREQ
HHHHHHCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438