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The map label for this gene is yheL

Identifier: 21672776

GI number: 21672776

Start: 565813

End: 566100

Strand: Reverse

Name: yheL

Synonym: BUsg511

Alternate gene names: 21672776

Gene position: 566100-565813 (Counterclockwise)

Preceding gene: 30410825

Following gene: 21672775

Centisome position: 88.25

GC content: 18.75

Gene sequence:

>288_bases
ATGCTGCATACTTTAATGAAATCTCCTTTTGAAACTAATATTTCTTTTTTTTCAGGTATGTTAAAAAAAACAGATGATTT
TTTAGCTTTACAAGATGGAGTTTTAATTGCATTAATTGATAATATTTTTTTAAAAAAAATTACTTTTTCTTCAGAAAATT
TATATGTTATAAAAGAAGATGTTTATGCTAGAGGAATTCATAAAAATATTTCTAGTAGTTTTATTTTAATTAGTTATATT
GATTTTGTTAATTTAACACTTAAAAATAAAAAACAAATAATTTGGTAA

Upstream 100 bases:

>100_bases
ATAATCAATTTATATTAAAAGTAAATATATTAAATTCATATTTTTTTCGTATAAAGTTAGATGATCATGATGCAATTATT
AATTTTTAAGGTACATAAAT

Downstream 100 bases:

>100_bases
AATATACTTATTTGTTCTTTGATTTATATATCCAATCCCAAAATTCTACATGATATAATATGACCTCAATTTTTTTTTAT
ATTATCTTAGTGTCACTCAT

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLHTLMKSPFETNISFFSGMLKKTDDFLALQDGVLIALIDNIFLKKITFSSENLYVIKEDVYARGIHKNISSSFILISYI
DFVNLTLKNKKQIIW

Sequences:

>Translated_95_residues
MLHTLMKSPFETNISFFSGMLKKTDDFLALQDGVLIALIDNIFLKKITFSSENLYVIKEDVYARGIHKNISSSFILISYI
DFVNLTLKNKKQIIW
>Mature_95_residues
MLHTLMKSPFETNISFFSGMLKKTDDFLALQDGVLIALIDNIFLKKITFSSENLYVIKEDVYARGIHKNISSSFILISYI
DFVNLTLKNKKQIIW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=41.0526315789474, Blast_Score=80, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_BUCAP (Q8K946)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660843.1
- ProteinModelPortal:   Q8K946
- SMR:   Q8K946
- EnsemblBacteria:   EBBUCT00000000294
- GeneID:   1005660
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg511
- GeneTree:   EBGT00050000008149
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- ProtClustDB:   PRK13510
- BioCyc:   BAPH198804:BUSG511-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10992; Mature: 10992

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTLMKSPFETNISFFSGMLKKTDDFLALQDGVLIALIDNIFLKKITFSSENLYVIKED
CCCCHHCCCCCCCHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHEEECCCCEEEEECC
VYARGIHKNISSSFILISYIDFVNLTLKNKKQIIW
HHHHHHCCCCCCCEEEEEEEHHHEEEECCCCCCCC
>Mature Secondary Structure
MLHTLMKSPFETNISFFSGMLKKTDDFLALQDGVLIALIDNIFLKKITFSSENLYVIKED
CCCCHHCCCCCCCHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHEEECCCCEEEEECC
VYARGIHKNISSSFILISYIDFVNLTLKNKKQIIW
HHHHHHCCCCCCCEEEEEEEHHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438