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The map label for this gene is rpoA

Identifier: 21672745

GI number: 21672745

Start: 547868

End: 548857

Strand: Reverse

Name: rpoA

Synonym: BUsg480

Alternate gene names: 21672745

Gene position: 548857-547868 (Counterclockwise)

Preceding gene: 21672746

Following gene: 21672744

Centisome position: 85.56

GC content: 31.31

Gene sequence:

>990_bases
ATGCAGAATTCTATTATTGGTTTTTTAAAACCACGTTTAGTTGAAATTGAACAAATTACTGCAACTCATACTAAAGTCAC
TTTAGAACCATTAGAAAGAGGTTTTGGTCATACATTAGGTAATGCCCTTCGTAGAATTCTTCTTTCTTCTATGCCAGGAT
ATGCTGTAACTGAAGTTGAAATTGATGGAGTGTTACACGAATATAGCACTAAAGAAGGTGTACAAGAAGATATTTTAGAA
ATTTTATTAAATTTAAAAGAATTAGCAGTAAAAGTACATGGAAAAGATGAAGTTTTTATGTCATTAAATAAATCAGGGAT
TGGTTCTGTCACTGCGTCTGATATAATTCACGATGGTGATGTAGAAATTATTAAACCAGATCATTTAATTTGCCATTTGA
CTGATGAAAATGCCTCTATTCAAATGAGAATTAAAGTACAACGTGGAAGAGGCTATATCCCCGCTTCTTCTAGAATCCAT
ATGGAAGATGACTTGAGACCAATAGGTTGTTTGTTAGTAGATGCATGCTATAGTCCAATCAATCGTATTTCTTATAATGT
AGAAGCTGCACGTGTAGAACAAAGAACAGATTTAGATAAATTAATTATCGAAATGAAAACAAATGGAACAATAGACCCAG
AAGAAGCTATTCGACGTGCTGCTACTATCTTATCTGAACAATTAGAAGCATTTGTTGATTTAAGAGATGTGAAAGAACCT
GAAATAAAAGAAGAAAAACCTGAATTTGAGCCTATTTTATTACGTCCTGTAGATGATTTAGAATTAACTGTTCGTTCTGC
AAATTGTCTTAAAGCTGAATCAATACATTATATTGGTGATTTAGTTCAAAGAACAGAAGTTGAACTTTTAAAAACTCCTA
ATTTAGGTAAAAAATCTTTAACTGAAATTAAGGATATATTAGCAGCTCGAAATTTATCTCTTGGAATGAAATTAGAAAAA
TGGCCTCCATCGAGTATTTTAGAAGAATAA

Upstream 100 bases:

>100_bases
AGATTTCCTGAACGTTCTGATTTGTCTGCAGAAATTAATGAGCATTTAATTGTTGAACTTTATTCTAAATAAAAAGTTCA
CTCTTAATAGAGAGGATATT

Downstream 100 bases:

>100_bases
TGTTAAATATTAACTTGATATTATTAATATTCTTATCTTTAAAAGGATAAATTTATGCGACATCGAAAAATTGGTCGTAA
ATTAAACTGCAAAAGTGATC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQNSIIGFLKPRLVEIEQITATHTKVTLEPLERGFGHTLGNALRRILLSSMPGYAVTEVEIDGVLHEYSTKEGVQEDILE
ILLNLKELAVKVHGKDEVFMSLNKSGIGSVTASDIIHDGDVEIIKPDHLICHLTDENASIQMRIKVQRGRGYIPASSRIH
MEDDLRPIGCLLVDACYSPINRISYNVEAARVEQRTDLDKLIIEMKTNGTIDPEEAIRRAATILSEQLEAFVDLRDVKEP
EIKEEKPEFEPILLRPVDDLELTVRSANCLKAESIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDILAARNLSLGMKLEK
WPPSSILEE

Sequences:

>Translated_329_residues
MQNSIIGFLKPRLVEIEQITATHTKVTLEPLERGFGHTLGNALRRILLSSMPGYAVTEVEIDGVLHEYSTKEGVQEDILE
ILLNLKELAVKVHGKDEVFMSLNKSGIGSVTASDIIHDGDVEIIKPDHLICHLTDENASIQMRIKVQRGRGYIPASSRIH
MEDDLRPIGCLLVDACYSPINRISYNVEAARVEQRTDLDKLIIEMKTNGTIDPEEAIRRAATILSEQLEAFVDLRDVKEP
EIKEEKPEFEPILLRPVDDLELTVRSANCLKAESIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDILAARNLSLGMKLEK
WPPSSILEE
>Mature_329_residues
MQNSIIGFLKPRLVEIEQITATHTKVTLEPLERGFGHTLGNALRRILLSSMPGYAVTEVEIDGVLHEYSTKEGVQEDILE
ILLNLKELAVKVHGKDEVFMSLNKSGIGSVTASDIIHDGDVEIIKPDHLICHLTDENASIQMRIKVQRGRGYIPASSRIH
MEDDLRPIGCLLVDACYSPINRISYNVEAARVEQRTDLDKLIIEMKTNGTIDPEEAIRRAATILSEQLEAFVDLRDVKEP
EIKEEKPEFEPILLRPVDDLELTVRSANCLKAESIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDILAARNLSLGMKLEK
WPPSSILEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=85.4103343465046, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_BUCAP (O69232)

Other databases:

- EMBL:   AE013218
- EMBL:   M74510
- RefSeq:   NP_660812.1
- ProteinModelPortal:   O69232
- EnsemblBacteria:   EBBUCT00000000018
- GeneID:   1005689
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg480
- GeneTree:   EBGT00050000008134
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   BAPH198804:BUSG480-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36940; Mature: 36940

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSIIGFLKPRLVEIEQITATHTKVTLEPLERGFGHTLGNALRRILLSSMPGYAVTEVE
CCCCEEECCCCCCEEHHHHHHHHHEEEHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSTKEGVQEDILEILLNLKELAVKVHGKDEVFMSLNKSGIGSVTASDIIHDGD
ECHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCHHHEEECCC
VEIIKPDHLICHLTDENASIQMRIKVQRGRGYIPASSRIHMEDDLRPIGCLLVDACYSPI
EEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
NRISYNVEAARVEQRTDLDKLIIEMKTNGTIDPEEAIRRAATILSEQLEAFVDLRDVKEP
HHHCCCCHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIKEEKPEFEPILLRPVDDLELTVRSANCLKAESIHYIGDLVQRTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TEIKDILAARNLSLGMKLEKWPPSSILEE
HHHHHHHHHCCCCCCCEECCCCCHHHHCC
>Mature Secondary Structure
MQNSIIGFLKPRLVEIEQITATHTKVTLEPLERGFGHTLGNALRRILLSSMPGYAVTEVE
CCCCEEECCCCCCEEHHHHHHHHHEEEHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSTKEGVQEDILEILLNLKELAVKVHGKDEVFMSLNKSGIGSVTASDIIHDGD
ECHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCHHHEEECCC
VEIIKPDHLICHLTDENASIQMRIKVQRGRGYIPASSRIHMEDDLRPIGCLLVDACYSPI
EEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
NRISYNVEAARVEQRTDLDKLIIEMKTNGTIDPEEAIRRAATILSEQLEAFVDLRDVKEP
HHHCCCCHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIKEEKPEFEPILLRPVDDLELTVRSANCLKAESIHYIGDLVQRTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TEIKDILAARNLSLGMKLEKWPPSSILEE
HHHHHHHHHCCCCCCCEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438