Click here to switch to the map view.

The map label for this gene is dnaX

Identifier: 21672731

GI number: 21672731

Start: 532404

End: 533495

Strand: Direct

Name: dnaX

Synonym: BUsg466

Alternate gene names: 21672731

Gene position: 532404-533495 (Clockwise)

Preceding gene: 21672730

Following gene: 21672732

Centisome position: 83.0

GC content: 25.0

Gene sequence:

>1092_bases
ATGAATTATCAAATACTAGCAAGAAAATGGCGTCCTCAATCTTTTAAAAAAATAATTGGTCAAAAATATATTGTTAAAGC
TATATCTAATGGATTTTCTCTTGGGAAAATTCATCATGCATGGTTATTATCTGGAACAAGAGGTGTAGGAAAAACTACAA
TTGCTCGTTTAATCGCTAAAAGTTTAAATTGTGAAATAGGTATCACATCTTTACCATGTAGAAAATGTACTATCTGTCAA
GAAATAGAAAAAGGTATTTGTCTCGATTTTATTGAAATAGATGCGGCTTCACGTACGAAAGTAGAAGAAATTCGAGAAAT
TTTAGATAACATTTACTACACTCCTTCTAAAAGTCGTTTTAAGGTATATTTAATTGACGAAGTTCATATGCTATCTAGAC
ATAGTTTTAATGCACTTCTTAAAACTCTTGAAGAACCTCCTCAACATATAAAATTTATTTTAGCTACAACAGATGTAGAA
AAAATACCTAAAACTATTAGATCACGTTGTTTACATTTTAAATTAAATATATTATCAGAAGAAGATATTTTTAATTTTTT
AAAACATATTCTCAAAAAGGGAGGTAATAATTTTGATGAAGAAGCATTAAAAATAATATCTGATTATGCTAATGGAAGTA
TGAGAGATGCGTTAAACCTATTAGAACATGCTATGCATTTAAGTAAAAATAATATAAATTTAAAAAATACAACTGAAATG
TTAGGTATACCAAATAAAAAACATGCTTTCTTACTAACAAAATTTTTATTAGAACAAGATTCAAAAAAAATGATGTGTTT
ATTAAACAAAATAAGTAAGATAGGCTTAGAATGGCAAAATATCTTAATAGAAATGATGCGTTTTTTACACCATATAGCTA
TGCTAAAATCTTATCCAAAAATATGGAATCAAATTTTTATAAAAAATAATGAAAATGAAATAAAAAAAATTGCAGAAAAT
AATAGCAAATATAATATTCAATTATGTTATAAAATCTTGTTAAAGGGAAGAAAAGAATTATTTTTTTCACCTAATCATAA
AATGGGAGTAGAAATGATTTTACTCCAAGCAATTACAGAAATAAAAAGATAA

Upstream 100 bases:

>100_bases
TTATAAAAATTAAATAAAATTTTTATAAAAAAAAGAGAATACTTTTACATTCTAAAAAATATTGATAGCATAATAATATT
TCTATAAAGAAAAATAATAT

Downstream 100 bases:

>100_bases
AAATTATTTATAGTATTAACTGTTATCAAATTTCATTTAAAAAAGAGAGAATCAATATGTTTACTAAAGGTGGATTAGGA
AATCTTATGAAACAAGCTCA

Product: DNA polymerase III subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNYQILARKWRPQSFKKIIGQKYIVKAISNGFSLGKIHHAWLLSGTRGVGKTTIARLIAKSLNCEIGITSLPCRKCTICQ
EIEKGICLDFIEIDAASRTKVEEIREILDNIYYTPSKSRFKVYLIDEVHMLSRHSFNALLKTLEEPPQHIKFILATTDVE
KIPKTIRSRCLHFKLNILSEEDIFNFLKHILKKGGNNFDEEALKIISDYANGSMRDALNLLEHAMHLSKNNINLKNTTEM
LGIPNKKHAFLLTKFLLEQDSKKMMCLLNKISKIGLEWQNILIEMMRFLHHIAMLKSYPKIWNQIFIKNNENEIKKIAEN
NSKYNIQLCYKILLKGRKELFFSPNHKMGVEMILLQAITEIKR

Sequences:

>Translated_363_residues
MNYQILARKWRPQSFKKIIGQKYIVKAISNGFSLGKIHHAWLLSGTRGVGKTTIARLIAKSLNCEIGITSLPCRKCTICQ
EIEKGICLDFIEIDAASRTKVEEIREILDNIYYTPSKSRFKVYLIDEVHMLSRHSFNALLKTLEEPPQHIKFILATTDVE
KIPKTIRSRCLHFKLNILSEEDIFNFLKHILKKGGNNFDEEALKIISDYANGSMRDALNLLEHAMHLSKNNINLKNTTEM
LGIPNKKHAFLLTKFLLEQDSKKMMCLLNKISKIGLEWQNILIEMMRFLHHIAMLKSYPKIWNQIFIKNNENEIKKIAEN
NSKYNIQLCYKILLKGRKELFFSPNHKMGVEMILLQAITEIKR
>Mature_363_residues
MNYQILARKWRPQSFKKIIGQKYIVKAISNGFSLGKIHHAWLLSGTRGVGKTTIARLIAKSLNCEIGITSLPCRKCTICQ
EIEKGICLDFIEIDAASRTKVEEIREILDNIYYTPSKSRFKVYLIDEVHMLSRHSFNALLKTLEEPPQHIKFILATTDVE
KIPKTIRSRCLHFKLNILSEEDIFNFLKHILKKGGNNFDEEALKIISDYANGSMRDALNLLEHAMHLSKNNINLKNTTEM
LGIPNKKHAFLLTKFLLEQDSKKMMCLLNKISKIGLEWQNILIEMMRFLHHIAMLKSYPKIWNQIFIKNNENEIKKIAEN
NSKYNIQLCYKILLKGRKELFFSPNHKMGVEMILLQAITEIKR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI194306571, Length=290, Percent_Identity=23.1034482758621, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI194306567, Length=290, Percent_Identity=23.1034482758621, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI6677723, Length=287, Percent_Identity=23.3449477351916, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194306569, Length=205, Percent_Identity=23.4146341463415, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4506489, Length=228, Percent_Identity=28.5087719298246, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786676, Length=359, Percent_Identity=50.6963788300836, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1787119, Length=233, Percent_Identity=27.4678111587983, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787341, Length=173, Percent_Identity=28.9017341040462, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17541988, Length=307, Percent_Identity=22.8013029315961, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71986063, Length=300, Percent_Identity=21.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324478, Length=294, Percent_Identity=23.1292517006803, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324039, Length=289, Percent_Identity=21.4532871972318, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17647857, Length=287, Percent_Identity=24.0418118466899, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921076, Length=304, Percent_Identity=22.3684210526316, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859927, Length=237, Percent_Identity=24.4725738396624, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO3X_BUCAP (Q8K983)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660798.1
- ProteinModelPortal:   Q8K983
- SMR:   Q8K983
- EnsemblBacteria:   EBBUCT00000000179
- GeneID:   1005699
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg466
- GeneTree:   EBGT00050000008070
- HOGENOM:   HBG729459
- OMA:   CETKLLA
- ProtClustDB:   PRK14961
- BioCyc:   BAPH198804:BUSG466-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763
- SMART:   SM00382
- TIGRFAMs:   TIGR02397

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 42187; Mature: 42187

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQILARKWRPQSFKKIIGQKYIVKAISNGFSLGKIHHAWLLSGTRGVGKTTIARLIAK
CCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNCEIGITSLPCRKCTICQEIEKGICLDFIEIDAASRTKVEEIREILDNIYYTPSKSRF
HCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHEECCCCCCHHHHHHHHHHHCEECCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPQHIKFILATTDVEKIPKTIRSRCLHFKLNILSE
EEEEEHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCHHHHHHHHHHHHHHHHEECCCH
EDIFNFLKHILKKGGNNFDEEALKIISDYANGSMRDALNLLEHAMHLSKNNINLKNTTEM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
LGIPNKKHAFLLTKFLLEQDSKKMMCLLNKISKIGLEWQNILIEMMRFLHHIAMLKSYPK
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IWNQIFIKNNENEIKKIAENNSKYNIQLCYKILLKGRKELFFSPNHKMGVEMILLQAITE
HHHHEEEECCHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHHHH
IKR
HCC
>Mature Secondary Structure
MNYQILARKWRPQSFKKIIGQKYIVKAISNGFSLGKIHHAWLLSGTRGVGKTTIARLIAK
CCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLNCEIGITSLPCRKCTICQEIEKGICLDFIEIDAASRTKVEEIREILDNIYYTPSKSRF
HCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHEECCCCCCHHHHHHHHHHHCEECCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPQHIKFILATTDVEKIPKTIRSRCLHFKLNILSE
EEEEEHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCHHHHHHHHHHHHHHHHEECCCH
EDIFNFLKHILKKGGNNFDEEALKIISDYANGSMRDALNLLEHAMHLSKNNINLKNTTEM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
LGIPNKKHAFLLTKFLLEQDSKKMMCLLNKISKIGLEWQNILIEMMRFLHHIAMLKSYPK
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IWNQIFIKNNENEIKKIAENNSKYNIQLCYKILLKGRKELFFSPNHKMGVEMILLQAITE
HHHHEEEECCHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHHHH
IKR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438