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The map label for this gene is cyoE

Identifier: 21672717

GI number: 21672717

Start: 514111

End: 514965

Strand: Reverse

Name: cyoE

Synonym: BUsg452

Alternate gene names: 21672717

Gene position: 514965-514111 (Counterclockwise)

Preceding gene: 30410824

Following gene: 30410823

Centisome position: 80.28

GC content: 21.87

Gene sequence:

>855_bases
ATGTTAAAAGATTATTTAGAAATAACGAAACCTCGTATTATTATTGGAAATATTATTTTAATAATAGGTAGTTTTTTGTT
TTCATCTTTTCCCTTTTTTAATGTTTTTTTATTTTTTTTTACTATTTTGGGAACGTCTCTAGTAATTGCTTCAAGCTGTA
TTTTTAATAATCTCATTGATATAGATATAGATACTAAGATGAATAGGACTAAGAATAGAGTTTTGGTAAAGAACCTAATA
AGCCCCACATCAGCATCGATTTTTGCTTCTTTTATTGGAATTGTGGGGTTTTTTATATTAGGTTTGTTTGTGAATATTCT
ATCAATGTTTTTATCTTTTATAGGATTTGTTATCTATGTTTTTTTCTATACATTTTTTTTAAAAAGAAAATCAATGTATT
CAACTTTTATTGGAAGTTTTTCCGGTTCTATTCCTTCTGTTATTGGACATACTGCTATTAGTAATAGTATTGATTTATTC
TGTTTCTTATTATTTATTATTTTTATATTTTGGCAAATGTCTCATTTTTATGCTATTGCGATTCTTTATATTAATGATTA
TAGAAAAGCAAATTTGCCCTTTTTTCCTGTAGTAAAAGGTATTTTAAAAACTAAAAAACATATTTTTTATTATATAACGT
GTTTTATAATCGCTAGTTCAATGTTAACTTTTTTAGGTTATTTGAGTTATATATTTCTTTTATTTTTTTCTTTTTTTAGT
TTTTATTGGTTATACATATCTTATTTAAGTATTCGAGAAAAAGATGATAGAAAATTTTCCAGTAAATTATTTTATTATTC
TATTGCAGTAGTTATATTATTTAATTTTTTAATATCTATAGATTTTATATTTTAA

Upstream 100 bases:

>100_bases
TATTGTTTTTATTATTGTGTTCGGTTCTATATGGATTATGTATAATTTAAATCATCATGTTATTTTGTAACTATTACTTA
CTTAAAAGTATTTTATCTAT

Downstream 100 bases:

>100_bases
ATTTTATAAACATCGTGAAGGTTTTGGTATACCAGCTATTTTACTTGCTTGTTTAGCTGGTCCTTCAGGAAAAAGTCTAA
ATAAATAAATACTATTGCTT

Product: protoheme IX farnesyltransferase

Products: NA

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLKDYLEITKPRIIIGNIILIIGSFLFSSFPFFNVFLFFFTILGTSLVIASSCIFNNLIDIDIDTKMNRTKNRVLVKNLI
SPTSASIFASFIGIVGFFILGLFVNILSMFLSFIGFVIYVFFYTFFLKRKSMYSTFIGSFSGSIPSVIGHTAISNSIDLF
CFLLFIIFIFWQMSHFYAIAILYINDYRKANLPFFPVVKGILKTKKHIFYYITCFIIASSMLTFLGYLSYIFLLFFSFFS
FYWLYISYLSIREKDDRKFSSKLFYYSIAVVILFNFLISIDFIF

Sequences:

>Translated_284_residues
MLKDYLEITKPRIIIGNIILIIGSFLFSSFPFFNVFLFFFTILGTSLVIASSCIFNNLIDIDIDTKMNRTKNRVLVKNLI
SPTSASIFASFIGIVGFFILGLFVNILSMFLSFIGFVIYVFFYTFFLKRKSMYSTFIGSFSGSIPSVIGHTAISNSIDLF
CFLLFIIFIFWQMSHFYAIAILYINDYRKANLPFFPVVKGILKTKKHIFYYITCFIIASSMLTFLGYLSYIFLLFFSFFS
FYWLYISYLSIREKDDRKFSSKLFYYSIAVVILFNFLISIDFIF
>Mature_284_residues
MLKDYLEITKPRIIIGNIILIIGSFLFSSFPFFNVFLFFFTILGTSLVIASSCIFNNLIDIDIDTKMNRTKNRVLVKNLI
SPTSASIFASFIGIVGFFILGLFVNILSMFLSFIGFVIYVFFYTFFLKRKSMYSTFIGSFSGSIPSVIGHTAISNSIDLF
CFLLFIIFIFWQMSHFYAIAILYINDYRKANLPFFPVVKGILKTKKHIFYYITCFIIASSMLTFLGYLSYIFLLFFSFFS
FYWLYISYLSIREKDDRKFSSKLFYYSIAVVILFNFLISIDFIF

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=246, Percent_Identity=32.1138211382114, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786631, Length=283, Percent_Identity=46.2897526501767, Blast_Score=268, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71998235, Length=267, Percent_Identity=26.9662921348315, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325085, Length=269, Percent_Identity=29.368029739777, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921064, Length=267, Percent_Identity=29.2134831460674, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYOE_BUCAP (Q8K997)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660784.1
- EnsemblBacteria:   EBBUCT00000000300
- GeneID:   1005713
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg452
- GeneTree:   EBGT00050000007931
- HOGENOM:   HBG656003
- OMA:   VGYCAVT
- ProtClustDB:   PRK04375
- BioCyc:   BAPH198804:BUSG452-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.-

Molecular weight: Translated: 33143; Mature: 33143

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc20604c)-; HASH(0xc1beb48)-; HASH(0xc1fd4c4)-; HASH(0xba2dbf8)-; HASH(0xc9866bc)-; HASH(0xc9911b0)-; HASH(0xc8f54e0)-; HASH(0xad0d3c0)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDYLEITKPRIIIGNIILIIGSFLFSSFPFFNVFLFFFTILGTSLVIASSCIFNNLID
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDIDTKMNRTKNRVLVKNLISPTSASIFASFIGIVGFFILGLFVNILSMFLSFIGFVIYV
EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFYTFFLKRKSMYSTFIGSFSGSIPSVIGHTAISNSIDLFCFLLFIIFIFWQMSHFYAIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
ILYINDYRKANLPFFPVVKGILKTKKHIFYYITCFIIASSMLTFLGYLSYIFLLFFSFFS
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYWLYISYLSIREKDDRKFSSKLFYYSIAVVILFNFLISIDFIF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLKDYLEITKPRIIIGNIILIIGSFLFSSFPFFNVFLFFFTILGTSLVIASSCIFNNLID
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDIDTKMNRTKNRVLVKNLISPTSASIFASFIGIVGFFILGLFVNILSMFLSFIGFVIYV
EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFYTFFLKRKSMYSTFIGSFSGSIPSVIGHTAISNSIDLFCFLLFIIFIFWQMSHFYAIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
ILYINDYRKANLPFFPVVKGILKTKKHIFYYITCFIIASSMLTFLGYLSYIFLLFFSFFS
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYWLYISYLSIREKDDRKFSSKLFYYSIAVVILFNFLISIDFIF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438