Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 21672688

GI number: 21672688

Start: 477625

End: 479136

Strand: Direct

Name: lysS

Synonym: BUsg422

Alternate gene names: 21672688

Gene position: 477625-479136 (Clockwise)

Preceding gene: 21672685

Following gene: 21672689

Centisome position: 74.46

GC content: 23.68

Gene sequence:

>1512_bases
ATGATTAAAAAAACAGATTTAAATAATAATATTTTTCAAAAAGAAAAACAAATAAGAAAAGAAAAACTGATTAATATGAA
AAAAAACGGATTTTCTTTTCCAAATAGTTTCAAAAAAAACACTAACTCTATCAAAATACATCAAGAATATGAAAATAAAA
CAATTAATGAACTTAAAGTTTTAAATGTTGAAGTTACGATTGCAGGTCGTATGATACAACGAAGAATTATGGGAAAAGCA
TCTTTTTTTACATTACAAGATATGGAAGGAAAAATACAAATTTATACAAGAGAAAAAGAGATTACATCAGATTTTTACAA
CAATCATTTTAAAAAATGGGATATTGGAGATATTCTAGGAATAGAAGGAATATTGTTTAAAACAAAAACAGGAGAATTAT
CTATTTTTTCTAAAAAATTAAAAATATTAACTAAATCTTTAAGACCCTTACCTGATAAATTTCACGGATTATCTAACCAA
GAAAAACGTTATAGAAAAAGATACTTAGATTTAATTAGTAATAATCAATTATTTAATATTTTTAAAAATCGTTCTAAAAT
AATCAGGTTCATTCGAAATTTTATGATAGAAAACAATTTTTTAGAAGTAGAAACTCCTATGTTACATAATATTCCTGGTG
GAGCAAATGCTCGACCTTTTATTACTTATCATAATGAAATTAATGAAGAAATGTACTTAAGAATAGCACCTGAATTATAT
TTAAAACAACTAATTGTGGGTGGTTTTGAACGTATTTTTGAACTCAATCGAAATTTTCGTAATGAAGGAGTTTCTGCTCG
ACATAATCCAGAATTTACTATGATGGAAGCATATATAGCTTATTCTAATTATGAAGATATGATGAATTTTACTGAAAATT
TACTAAAAAATATAATCAAATCAATTTGTGGAAAAAGTGAAATAAAATATAATAAGTATTACTTAAATTTTAATATTCCG
TTTAAAAAATTAACGATGAAAGAATCTATTTTGCAATTTAATTCAAATATTTGTTTATCAGATTTAAAAAATTTACAAAA
AATTAAAAAAATTGCAAATAATATTGGTATCGAAATAAAAGACAATTGGAATATAGGTTATATAGAAAATGAAATTTTTG
AAAAAACAGTAGAAAAAAACCTTATTCAACCTACTTTTATTACTGAATATCCAGTGGAAGTTTCTCCCCTAGCTAGACGT
AACGATTTTAATTCTAATGTAACAGATAGATTTGAACTTTTTATTGCTGGATATGAAATTGGAAATGGATTTTCTGAATT
AAACGATTCAGAAGATCAAAAAAATAGATTTTTAAATCAAATGAAAATAGCAGAAAAAGAAAAAAATAAAGATATGTTGT
ATGATGAAAATTACATAGAGGCATTAAAATACGGTTTACCTCCTACTTCAGGTTTAGGAATTGGAATTGATCGTCTGATT
ATGATATTAACTAATCAAATAAGCATTCGGGACGTTATTTTATTTCCAACATTACGTTCATTTAAAAAATAA

Upstream 100 bases:

>100_bases
TTAGAACAGGAGTAGAAAAATATAATGTACAATCTGTATTAGATGGTGACTTAGACGATTTTATTGAACAAAGTTTAATA
ATGGGATTATAGAAAATAAA

Downstream 100 bases:

>100_bases
TTCTTATTTTTTTATAAATTTATTTTGAGTAGGTGTGTAATTATACAATAATAAAATAGGAAAAAAAATGCCAGAATTAT
TAAATAAAACTAAAACAAAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MIKKTDLNNNIFQKEKQIRKEKLINMKKNGFSFPNSFKKNTNSIKIHQEYENKTINELKVLNVEVTIAGRMIQRRIMGKA
SFFTLQDMEGKIQIYTREKEITSDFYNNHFKKWDIGDILGIEGILFKTKTGELSIFSKKLKILTKSLRPLPDKFHGLSNQ
EKRYRKRYLDLISNNQLFNIFKNRSKIIRFIRNFMIENNFLEVETPMLHNIPGGANARPFITYHNEINEEMYLRIAPELY
LKQLIVGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMNFTENLLKNIIKSICGKSEIKYNKYYLNFNIP
FKKLTMKESILQFNSNICLSDLKNLQKIKKIANNIGIEIKDNWNIGYIENEIFEKTVEKNLIQPTFITEYPVEVSPLARR
NDFNSNVTDRFELFIAGYEIGNGFSELNDSEDQKNRFLNQMKIAEKEKNKDMLYDENYIEALKYGLPPTSGLGIGIDRLI
MILTNQISIRDVILFPTLRSFKK

Sequences:

>Translated_503_residues
MIKKTDLNNNIFQKEKQIRKEKLINMKKNGFSFPNSFKKNTNSIKIHQEYENKTINELKVLNVEVTIAGRMIQRRIMGKA
SFFTLQDMEGKIQIYTREKEITSDFYNNHFKKWDIGDILGIEGILFKTKTGELSIFSKKLKILTKSLRPLPDKFHGLSNQ
EKRYRKRYLDLISNNQLFNIFKNRSKIIRFIRNFMIENNFLEVETPMLHNIPGGANARPFITYHNEINEEMYLRIAPELY
LKQLIVGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMNFTENLLKNIIKSICGKSEIKYNKYYLNFNIP
FKKLTMKESILQFNSNICLSDLKNLQKIKKIANNIGIEIKDNWNIGYIENEIFEKTVEKNLIQPTFITEYPVEVSPLARR
NDFNSNVTDRFELFIAGYEIGNGFSELNDSEDQKNRFLNQMKIAEKEKNKDMLYDENYIEALKYGLPPTSGLGIGIDRLI
MILTNQISIRDVILFPTLRSFKK
>Mature_503_residues
MIKKTDLNNNIFQKEKQIRKEKLINMKKNGFSFPNSFKKNTNSIKIHQEYENKTINELKVLNVEVTIAGRMIQRRIMGKA
SFFTLQDMEGKIQIYTREKEITSDFYNNHFKKWDIGDILGIEGILFKTKTGELSIFSKKLKILTKSLRPLPDKFHGLSNQ
EKRYRKRYLDLISNNQLFNIFKNRSKIIRFIRNFMIENNFLEVETPMLHNIPGGANARPFITYHNEINEEMYLRIAPELY
LKQLIVGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMNFTENLLKNIIKSICGKSEIKYNKYYLNFNIP
FKKLTMKESILQFNSNICLSDLKNLQKIKKIANNIGIEIKDNWNIGYIENEIFEKTVEKNLIQPTFITEYPVEVSPLARR
NDFNSNVTDRFELFIAGYEIGNGFSELNDSEDQKNRFLNQMKIAEKEKNKDMLYDENYIEALKYGLPPTSGLGIGIDRLI
MILTNQISIRDVILFPTLRSFKK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=501, Percent_Identity=37.5249500998004, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI5031815, Length=505, Percent_Identity=37.4257425742574, Blast_Score=345, Evalue=8e-95,
Organism=Homo sapiens, GI45439306, Length=482, Percent_Identity=21.9917012448133, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4758762, Length=335, Percent_Identity=23.2835820895522, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI40789249, Length=258, Percent_Identity=25.968992248062, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789256, Length=498, Percent_Identity=58.0321285140562, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=491, Percent_Identity=56.2118126272912, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=29.5180722891566, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788173, Length=257, Percent_Identity=27.2373540856031, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=452, Percent_Identity=39.3805309734513, Blast_Score=324, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17535927, Length=452, Percent_Identity=39.3805309734513, Blast_Score=324, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=452, Percent_Identity=39.3805309734513, Blast_Score=323, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI32566633, Length=265, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=455, Percent_Identity=40.6593406593407, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6324256, Length=426, Percent_Identity=32.3943661971831, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323011, Length=355, Percent_Identity=25.6338028169014, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=341, Percent_Identity=23.4604105571848, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325153, Length=136, Percent_Identity=33.8235294117647, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640851, Length=499, Percent_Identity=36.2725450901804, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24640849, Length=481, Percent_Identity=37.2141372141372, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17136276, Length=463, Percent_Identity=22.2462203023758, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24584738, Length=289, Percent_Identity=25.2595155709343, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_BUCAP (Q8K9C5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660755.1
- ProteinModelPortal:   Q8K9C5
- SMR:   Q8K9C5
- EnsemblBacteria:   EBBUCT00000000532
- GeneID:   1005741
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg422
- GeneTree:   EBGT00050000007970
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   BAPH198804:BUSG422-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 59317; Mature: 59317

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTDLNNNIFQKEKQIRKEKLINMKKNGFSFPNSFKKNTNSIKIHQEYENKTINELKV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEEEHHCCCCCCCEEE
LNVEVTIAGRMIQRRIMGKASFFTLQDMEGKIQIYTREKEITSDFYNNHFKKWDIGDILG
EEEEEEEHHHHHHHHHHCCHHEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHC
IEGILFKTKTGELSIFSKKLKILTKSLRPLPDKFHGLSNQEKRYRKRYLDLISNNQLFNI
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHH
FKNRSKIIRFIRNFMIENNFLEVETPMLHNIPGGANARPFITYHNEINEEMYLRIAPELY
HHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEECHHHH
LKQLIVGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMNFTENLLKNIIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SICGKSEIKYNKYYLNFNIPFKKLTMKESILQFNSNICLSDLKNLQKIKKIANNIGIEIK
HHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
DNWNIGYIENEIFEKTVEKNLIQPTFITEYPVEVSPLARRNDFNSNVTDRFELFIAGYEI
CCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHCCCCCCCCCCEEEEEEEEEEC
GNGFSELNDSEDQKNRFLNQMKIAEKEKNKDMLYDENYIEALKYGLPPTSGLGIGIDRLI
CCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
MILTNQISIRDVILFPTLRSFKK
HHHHCCCCEEHEEECCCHHHHCC
>Mature Secondary Structure
MIKKTDLNNNIFQKEKQIRKEKLINMKKNGFSFPNSFKKNTNSIKIHQEYENKTINELKV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEEEHHCCCCCCCEEE
LNVEVTIAGRMIQRRIMGKASFFTLQDMEGKIQIYTREKEITSDFYNNHFKKWDIGDILG
EEEEEEEHHHHHHHHHHCCHHEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHC
IEGILFKTKTGELSIFSKKLKILTKSLRPLPDKFHGLSNQEKRYRKRYLDLISNNQLFNI
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHH
FKNRSKIIRFIRNFMIENNFLEVETPMLHNIPGGANARPFITYHNEINEEMYLRIAPELY
HHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEECHHHH
LKQLIVGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMNFTENLLKNIIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SICGKSEIKYNKYYLNFNIPFKKLTMKESILQFNSNICLSDLKNLQKIKKIANNIGIEIK
HHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
DNWNIGYIENEIFEKTVEKNLIQPTFITEYPVEVSPLARRNDFNSNVTDRFELFIAGYEI
CCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHCCCCCCCCCCEEEEEEEEEEC
GNGFSELNDSEDQKNRFLNQMKIAEKEKNKDMLYDENYIEALKYGLPPTSGLGIGIDRLI
CCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
MILTNQISIRDVILFPTLRSFKK
HHHHCCCCEEHEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438