Click here to switch to the map view.

The map label for this gene is nusA

Identifier: 21672641

GI number: 21672641

Start: 418924

End: 420411

Strand: Reverse

Name: nusA

Synonym: BUsg366

Alternate gene names: 21672641

Gene position: 420411-418924 (Counterclockwise)

Preceding gene: 21672642

Following gene: 21672640

Centisome position: 65.54

GC content: 32.66

Gene sequence:

>1488_bases
ATGAATAAAGAAATCTTAGCTGTTGTAGAAGCTGTGTCTAATGAAAAATCACTGCCACGTGAAAAAATTTTTGAAGCTTT
AGAAATTGCATTAGCAACGGCAACTAAGAAAAAATATGAACAAGAAATTGATGTAAGAGTTAGTATAAATCGTAAAACTG
GAAATTTTAGTACCTTTAGACGATGGATGGTAGTAGATATTGTTGTTCATCCAACAAAAGAGATTACTTTAGAAGCAGCT
TGTTTTGAAGGTGAACAAGTTAAAATTAATGATTATGTAGAAGATAAAATTGAATCTGTAAATTTTGATAGAATAACAAC
TCAAACTGCTAAACAAGTCATTGTTCAAAAAGTACGTGAAGCAGAACGAGCAATGCTAGTCGATCAATTTCGTAAACATA
TTGGTCAAATCATCACCGGTATAGTTAAAAAAATTAGTCGAGATAATTTGACTTTAGATTTAGGTAATAATGCCGAAGCT
TTAATTTTACGTGAAGGTATGTTACCTCGAGAAAATTTTCGTCCTGGAGATCGTATTCGTGGTATTTTATACGGAGTATA
TCCAGAAGCTCGTGGCGCACAGTTGTTTCTGAGTCGTTCAAAAACTGAAATGCTTATTGAGCTTTTTCGTATAGAAGTTC
CCGAAATTGGAGAAGAAGTTATTGAAATTAAAGCAGCTGCACGTGATCCAGGTTCTCGTGCTAAAATTGCAGTAAAAACC
AATGATAAACGAATTGATCCAATAGGAGCTTGTGTAGGAATGAGAGGTGCACGAGTACAAGCTGTATCTAGTGAATTATG
TGGAGAACGAATTGATATTATCTTATGGGATGATAATCCTGCTCAATTTGTCATTAACGCTATGGCTCCTGCTGATGTCG
TTTCTATTATAGTAGATGAAGATCACCATACTATGGACATTGCTGTAGACTCGAGTAATTTAGCACAAGCGATTGGACGT
AATGGTCAAAATGTTCGTTTAGCTTCTCAGATTAGTGGTTGGGAACTTAATGTTATGACTACAGAAGATCTTAGATCTAA
ACACAAAGAAGAAGCTTATGCTGCTTTTAATATTTTTAAGAAAAATTTAAATGTTAGTGAAAAAGTCATTAAAACTTTAG
TTAAAGAAGGTTTTTCTTCTCTCGAAGAATTAGCGTATATACCACTTAATGAGTTGTTAGAAATTAATAATTTAACAGAG
AGAGAAGTGCAATTAGTTCGTGAAGGCGCTAAAAATGGATTGCTTCTTCTTGAGTTAGATCAAAAAAATCAGATCAAAAA
TAAAAAAATTGAACAAGCTTTATTGAATGTAAATGGTATGAATGAGCTATTAGCTTTAAAGTTAGCAGAAAAAAATATAT
TTACTGTGGAAGAATTAGCTAATCAGGGAATAGATGATTTAATTGATATTGAAAATTTAAATTCTGAGCAAGCTGGTTTG
TTAATTATGGCAGCTCGTAATATATGTTGGTTTAGCAGCAAAGTCTGA

Upstream 100 bases:

>100_bases
ATTTTTATTAAAAATAGGGTGATCTTACCTAAAACCCCGAAATTTCGGGGTTTTTTATTATAATCTTTATTTGAAAATTT
TTAGTTTGAGGTTGTCTAAG

Downstream 100 bases:

>100_bases
TAATAGGAAAAAAAGTATGGTTGATATCAGTTTAAAAATTTTATCAAATGAAATGAAAATATCTATTAAAGAATTAATAA
AGACATTATCTGAAATTAGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNKEILAVVEAVSNEKSLPREKIFEALEIALATATKKKYEQEIDVRVSINRKTGNFSTFRRWMVVDIVVHPTKEITLEAA
CFEGEQVKINDYVEDKIESVNFDRITTQTAKQVIVQKVREAERAMLVDQFRKHIGQIITGIVKKISRDNLTLDLGNNAEA
LILREGMLPRENFRPGDRIRGILYGVYPEARGAQLFLSRSKTEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPIGACVGMRGARVQAVSSELCGERIDIILWDDNPAQFVINAMAPADVVSIIVDEDHHTMDIAVDSSNLAQAIGR
NGQNVRLASQISGWELNVMTTEDLRSKHKEEAYAAFNIFKKNLNVSEKVIKTLVKEGFSSLEELAYIPLNELLEINNLTE
REVQLVREGAKNGLLLLELDQKNQIKNKKIEQALLNVNGMNELLALKLAEKNIFTVEELANQGIDDLIDIENLNSEQAGL
LIMAARNICWFSSKV

Sequences:

>Translated_495_residues
MNKEILAVVEAVSNEKSLPREKIFEALEIALATATKKKYEQEIDVRVSINRKTGNFSTFRRWMVVDIVVHPTKEITLEAA
CFEGEQVKINDYVEDKIESVNFDRITTQTAKQVIVQKVREAERAMLVDQFRKHIGQIITGIVKKISRDNLTLDLGNNAEA
LILREGMLPRENFRPGDRIRGILYGVYPEARGAQLFLSRSKTEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPIGACVGMRGARVQAVSSELCGERIDIILWDDNPAQFVINAMAPADVVSIIVDEDHHTMDIAVDSSNLAQAIGR
NGQNVRLASQISGWELNVMTTEDLRSKHKEEAYAAFNIFKKNLNVSEKVIKTLVKEGFSSLEELAYIPLNELLEINNLTE
REVQLVREGAKNGLLLLELDQKNQIKNKKIEQALLNVNGMNELLALKLAEKNIFTVEELANQGIDDLIDIENLNSEQAGL
LIMAARNICWFSSKV
>Mature_495_residues
MNKEILAVVEAVSNEKSLPREKIFEALEIALATATKKKYEQEIDVRVSINRKTGNFSTFRRWMVVDIVVHPTKEITLEAA
CFEGEQVKINDYVEDKIESVNFDRITTQTAKQVIVQKVREAERAMLVDQFRKHIGQIITGIVKKISRDNLTLDLGNNAEA
LILREGMLPRENFRPGDRIRGILYGVYPEARGAQLFLSRSKTEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPIGACVGMRGARVQAVSSELCGERIDIILWDDNPAQFVINAMAPADVVSIIVDEDHHTMDIAVDSSNLAQAIGR
NGQNVRLASQISGWELNVMTTEDLRSKHKEEAYAAFNIFKKNLNVSEKVIKTLVKEGFSSLEELAYIPLNELLEINNLTE
REVQLVREGAKNGLLLLELDQKNQIKNKKIEQALLNVNGMNELLALKLAEKNIFTVEELANQGIDDLIDIENLNSEQAGL
LIMAARNICWFSSKV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=74.4939271255061, Blast_Score=760, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_BUCAP (Q8K9H0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660708.1
- ProteinModelPortal:   Q8K9H0
- EnsemblBacteria:   EBBUCT00000000388
- GeneID:   1005791
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg366
- GeneTree:   EBGT00050000007908
- HOGENOM:   HBG529269
- OMA:   RSKISVY
- ProtClustDB:   PRK09202
- BioCyc:   BAPH198804:BUSG366-MONOMER
- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953
- TIGRFAMs:   TIGR01954

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF47794 Rad51_N; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 55644; Mature: 55644

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALEIALATATKKKYEQEIDVRVSINRKTGNFSTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RWMVVDIVVHPTKEITLEAACFEGEQVKINDYVEDKIESVNFDRITTQTAKQVIVQKVRE
HEEEEEEEEECCHHEEEEEEECCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AERAMLVDQFRKHIGQIITGIVKKISRDNLTLDLGNNAEALILREGMLPRENFRPGDRIR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHC
GILYGVYPEARGAQLFLSRSKTEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPIGACVGMRGARVQAVSSELCGERIDIILWDDNPAQFVINAMAPADVVSIIVDE
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHEEEECCCCCCEEEEEECC
DHHTMDIAVDSSNLAQAIGRNGQNVRLASQISGWELNVMTTEDLRSKHKEEAYAAFNIFK
CCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHH
KNLNVSEKVIKTLVKEGFSSLEELAYIPLNELLEINNLTEREVQLVREGAKNGLLLLELD
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC
QKNQIKNKKIEQALLNVNGMNELLALKLAEKNIFTVEELANQGIDDLIDIENLNSEQAGL
CCCCHHHHHHHHHHHCCCCCHHHHHEEECCCCCEEHHHHHHCCCHHHHHHCCCCCCCCCE
LIMAARNICWFSSKV
EEEEECCEEEECCCC
>Mature Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALEIALATATKKKYEQEIDVRVSINRKTGNFSTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RWMVVDIVVHPTKEITLEAACFEGEQVKINDYVEDKIESVNFDRITTQTAKQVIVQKVRE
HEEEEEEEEECCHHEEEEEEECCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AERAMLVDQFRKHIGQIITGIVKKISRDNLTLDLGNNAEALILREGMLPRENFRPGDRIR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHC
GILYGVYPEARGAQLFLSRSKTEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPIGACVGMRGARVQAVSSELCGERIDIILWDDNPAQFVINAMAPADVVSIIVDE
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHEEEECCCCCCEEEEEECC
DHHTMDIAVDSSNLAQAIGRNGQNVRLASQISGWELNVMTTEDLRSKHKEEAYAAFNIFK
CCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHH
KNLNVSEKVIKTLVKEGFSSLEELAYIPLNELLEINNLTEREVQLVREGAKNGLLLLELD
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC
QKNQIKNKKIEQALLNVNGMNELLALKLAEKNIFTVEELANQGIDDLIDIENLNSEQAGL
CCCCHHHHHHHHHHHCCCCCHHHHHEEECCCCCEEHHHHHHCCCHHHHHHCCCCCCCCCE
LIMAARNICWFSSKV
EEEEECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438