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The map label for this gene is truB

Identifier: 21672638

GI number: 21672638

Start: 414910

End: 415848

Strand: Reverse

Name: truB

Synonym: BUsg363

Alternate gene names: 21672638

Gene position: 415848-414910 (Counterclockwise)

Preceding gene: 21672639

Following gene: 21672637

Centisome position: 64.83

GC content: 26.84

Gene sequence:

>939_bases
ATGTTTTTTCATAAAAAACGTAATGTTAATGGATTTTTATTGTTGGACAAGCCTAAGGGAATGACTTCTAACAATGTTTT
ACAGAAAGTAAAAATTATTTTTAAGGCTAAAAAAGCAGGCTATATTGGTACCTTAGATCCCTTAGCTACTGGAATTTTAC
CGATTTGTTTTGGTGAAGCTACAAAATTTTCTAATTATTTGAATGCATCAGACAAACACTATAATGTAATTGCTAGGTTG
GGAGAAAAAACATCTACTTCGGATTCTGATGGAATAATTGTAAGAAAAAGACCCATTTTATTTACGCCTATTCAACTTAG
TTTAGCTTTAAAAGCACTGACAGGTTCAGTTAATCAAATACCTTCAATGTACTCAGCTGTTAAATATAAAGGTATTCCTT
TGTATAAATATGCACGACAGGGAATAAATGTTAAACGTAATATTAGATGCGTTTTTATATATAAGATTGATCTTGTTGAT
CAAAAAGATAATTGGATTGAATTAAATGTGCATTGTTCAAAAGGTACTTATATTCGAACACTTATTGAAGATTTAGGTGA
AAAATTATTTTGTGGAGCTCACGTAATTAGATTACGTCGTTTAAAAATAGGATTACTTTCTTATTCTAAATTAGTCAAGC
TATCTTTTTTAGAAAATTTATTAAATGAAAAAAATGTTGTAAAAATAAATTTTTTTAAAAAAATAGATGATTTGCTAATG
CCAGTTGATACTCCAGTTTATTTTTTGCCTAAGATATATATTTCTATTGAAAAATTATCTGTTTTTAGATTAGGACAAAA
AGTTAATTTTTCATCTTCTATCACAAATGGTTTAGTCCGTGTTTTTGAAAAAGATAATAATACATTTATCGGATTAGGAA
AGATTAATTCTGAAAAAACGTTAATTCCGTATCGATTAGTTTCTATGTTAACTAATTAA

Upstream 100 bases:

>100_bases
GATGATTCCTTTTTTAAAGGTAACAAAATTTCTAAATTGTTAAATATATTAACAGAGAAGAATAACATTACATTATGAAT
AAAAATATAAGGATAGGTAA

Downstream 100 bases:

>100_bases
ATTGATCTATATTAAATTTAATAATAGCATGATATTATTTTTTAAATAATTTTATTAAATTATAATTAATTTTACATAGT
TTCAAATATATGGAGTATCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFFHKKRNVNGFLLLDKPKGMTSNNVLQKVKIIFKAKKAGYIGTLDPLATGILPICFGEATKFSNYLNASDKHYNVIARL
GEKTSTSDSDGIIVRKRPILFTPIQLSLALKALTGSVNQIPSMYSAVKYKGIPLYKYARQGINVKRNIRCVFIYKIDLVD
QKDNWIELNVHCSKGTYIRTLIEDLGEKLFCGAHVIRLRRLKIGLLSYSKLVKLSFLENLLNEKNVVKINFFKKIDDLLM
PVDTPVYFLPKIYISIEKLSVFRLGQKVNFSSSITNGLVRVFEKDNNTFIGLGKINSEKTLIPYRLVSMLTN

Sequences:

>Translated_312_residues
MFFHKKRNVNGFLLLDKPKGMTSNNVLQKVKIIFKAKKAGYIGTLDPLATGILPICFGEATKFSNYLNASDKHYNVIARL
GEKTSTSDSDGIIVRKRPILFTPIQLSLALKALTGSVNQIPSMYSAVKYKGIPLYKYARQGINVKRNIRCVFIYKIDLVD
QKDNWIELNVHCSKGTYIRTLIEDLGEKLFCGAHVIRLRRLKIGLLSYSKLVKLSFLENLLNEKNVVKINFFKKIDDLLM
PVDTPVYFLPKIYISIEKLSVFRLGQKVNFSSSITNGLVRVFEKDNNTFIGLGKINSEKTLIPYRLVSMLTN
>Mature_312_residues
MFFHKKRNVNGFLLLDKPKGMTSNNVLQKVKIIFKAKKAGYIGTLDPLATGILPICFGEATKFSNYLNASDKHYNVIARL
GEKTSTSDSDGIIVRKRPILFTPIQLSLALKALTGSVNQIPSMYSAVKYKGIPLYKYARQGINVKRNIRCVFIYKIDLVD
QKDNWIELNVHCSKGTYIRTLIEDLGEKLFCGAHVIRLRRLKIGLLSYSKLVKLSFLENLLNEKNVVKINFFKKIDDLLM
PVDTPVYFLPKIYISIEKLSVFRLGQKVNFSSSITNGLVRVFEKDNNTFIGLGKINSEKTLIPYRLVSMLTN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=215, Percent_Identity=32.093023255814, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4503337, Length=204, Percent_Identity=30.8823529411765, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI215599015, Length=204, Percent_Identity=30.8823529411765, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI2367200, Length=303, Percent_Identity=51.4851485148515, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17553978, Length=223, Percent_Identity=29.1479820627803, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=200, Percent_Identity=32.5, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=31.2169312169312, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BUCAP (Q8K9H3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660705.1
- ProteinModelPortal:   Q8K9H3
- SMR:   Q8K9H3
- EnsemblBacteria:   EBBUCT00000000138
- GeneID:   1005555
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg363
- GeneTree:   EBGT00050000007789
- HOGENOM:   HBG397258
- OMA:   TDDMQGE
- ProtClustDB:   PRK05033
- BioCyc:   BAPH198804:BUSG363-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35452; Mature: 35452

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: ACT_SITE 46-46 BINDING 74-74 BINDING 177-177 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFHKKRNVNGFLLLDKPKGMTSNNVLQKVKIIFKAKKAGYIGTLDPLATGILPICFGEA
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHCCCCH
TKFSNYLNASDKHYNVIARLGEKTSTSDSDGIIVRKRPILFTPIQLSLALKALTGSVNQI
HHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHCCHHHH
PSMYSAVKYKGIPLYKYARQGINVKRNIRCVFIYKIDLVDQKDNWIELNVHCSKGTYIRT
HHHHHHHHCCCCCHHHHHHCCCCEECCEEEEEEEEEEEEECCCCEEEEEEEECCCCHHHH
LIEDLGEKLFCGAHVIRLRRLKIGLLSYSKLVKLSFLENLLNEKNVVKINFFKKIDDLLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHC
PVDTPVYFLPKIYISIEKLSVFRLGQKVNFSSSITNGLVRVFEKDNNTFIGLGKINSEKT
CCCCCCEEECEEEEEEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCEEEEEEEECCCCE
LIPYRLVSMLTN
EHHHHHHHHHCC
>Mature Secondary Structure
MFFHKKRNVNGFLLLDKPKGMTSNNVLQKVKIIFKAKKAGYIGTLDPLATGILPICFGEA
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHCCCCH
TKFSNYLNASDKHYNVIARLGEKTSTSDSDGIIVRKRPILFTPIQLSLALKALTGSVNQI
HHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHCCHHHH
PSMYSAVKYKGIPLYKYARQGINVKRNIRCVFIYKIDLVDQKDNWIELNVHCSKGTYIRT
HHHHHHHHCCCCCHHHHHHCCCCEECCEEEEEEEEEEEEECCCCEEEEEEEECCCCHHHH
LIEDLGEKLFCGAHVIRLRRLKIGLLSYSKLVKLSFLENLLNEKNVVKINFFKKIDDLLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHC
PVDTPVYFLPKIYISIEKLSVFRLGQKVNFSSSITNGLVRVFEKDNNTFIGLGKINSEKT
CCCCCCEEECEEEEEEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCEEEEEEEECCCCE
LIPYRLVSMLTN
EHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438