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The map label for this gene is pnp

Identifier: 21672636

GI number: 21672636

Start: 412142

End: 414265

Strand: Reverse

Name: pnp

Synonym: BUsg361

Alternate gene names: 21672636

Gene position: 414265-412142 (Counterclockwise)

Preceding gene: 21672637

Following gene: 21672635

Centisome position: 64.58

GC content: 30.65

Gene sequence:

>2124_bases
TTGCTAAATCCCATTGTTCGTAAATTTCAATATGGCCAACATACAATTACCTTAGAAACAGGTATAATGGCTCGCCAAGC
AACAGCAGCTGTCATGGCTAGCATGGATGATACAGCTGTTTTTGTTACTGTTGTTGGAGAAAAAACAACAAATTCTAGTC
AAAAATTTTTTCCTCTTACAGTAAATTACCAGGAAAGAACATATGCTGTAGGTCGAATACCTGGAGGTTTTTTTAGAAGA
GAAGGACGTCCAAGTGAAAATGAAATATTAACTGCTCGACTAATTGATAGACCAATTCGACCCTTATTTCCAAAAGGTTT
TTGTAATGAAATTCAAATAATTGCTACGGTAGTATCAGTTAATCCTCAAATAAATCCTGACATAATTTCTATTATAGGTG
CTTCAGCAGCATTAAGTCTATCAGGAATACCATTTTATGGACCAATTGGAGCTGCTAGAGTTGGTTTTGTTAATAATCAA
TATGTTTTAAATCCTACAATTGACGATATGAAATCTAGTTTTTTAGATTTAGTTGTCTCGGGTACTCAAAATGCTGTTTT
AATGGTAGAAGCAGAGTCAAAAGTGTTAAGTGAAGATAAAATTCTTGGTGCTATTATGTTTGGTCATCAACAACAACAAG
TTGTTATTAACAATATTCGTTCTTTATCTAATGAAGCCAGCAAATTACCTTGGATAATATCTTATCCTGAAATAAATACA
GAATTAGAATTAAAAATAACCAAATTGGCTGAAAAAGACATAAGTAATGCTTATTTAATATTTAATAAGCAAGAAAGATA
TGAAAAACTAAATTTTCTTAAGGAAGAAATAATAAAATTATTTTTTAGTGAAAACTCAAATATAGATATATCAGAGATTG
AAGATATTTTTGAAAAAATTGAAAAAAATATTGTAAGAAAACGTATACTAAATAATGAAAATCGTATTGATGGACGAGAG
AAAGATATGATTCGTGCATTAGATATCAGAACAGGTGTATTACCGCGTACACATGGTTCTTCTTTATTCACTAGAGGTGA
AACGCAATCTTTAGTCTCTGTTACCTTAGGAACATCTCGAGATGCACAAAATTTAGATGAATTACTAGGGGATAAAACAG
ATAATTTTTTATTTCACTATAATTTCCCTCCTTATTCTGTTGGAGAAATTGGAATAGTAGGATCACCTAAAAGACGAGAA
ATTGGACATGGTCGTTTAGCTAAAAGAAGTCTTTTAGCAGTTATGCCTAAATTAGATGATTTTCCTTATACTATAAGAAT
AGTATCTGAAATTACTGAATCGAATGGTTCTTCTTCTATGGCTTCTGTTTGCGGTGCATCTTTGGCTTTAATGGATGCTG
GAGTACCTATAAAATCTGCTGTTGCAGGAATATCTATGGGTCTAGTTAAGGAGGGAGATAAATATGTTTTACTTTCAGAT
ATTTTAGGTGATGAAGATCATTTAGGAGATATGGACTTTAAAGTTTCTGGAACAGAAGAAGGTATTACCGCTTTACAAAT
GGATATAAAAATAGAAGGGATTACTAATGAAATTATGCGTATTGCACTAAATAAAGCGAAATCTGCTCGATTGCATATTT
TAAATGTTATGAAACAAGCATTAAGTAAACCTAGAAACGAAATTTCTGAATTTGCACCTCGTATTCATAAAATTAAAATA
AACCCTGAAAAAATTAAAGATGTAATAGGTAAGGGGGGTTCTGTAATTCGCATGTTAACTGAAGAAACAGGTACAATAAT
TGAAATAGAAGATGATGGTACAATTAAGATATCTGCAACAATTGGAGAAAAAGCAAAAAATGCTATTCGTAGAATTGAAG
AAATTACAGCAGAAATTGAAGTTGGACGTATTTATTCTGGAAAAGTTACTCGTATTGTTGATTTTGGTGCTTTTATTTCA
ATAGGTATTGGAAAAGAAGGATTAGTACATATTTCACAAATTTCTAATAAACGCGTTGAAAAAGTTTCTGATCATTTGAC
AGTTGATCAGATAATTTCAGTAAAAGTATTGGAAATAGATCGTCAGGGTCGTTTAAGATTAAGTATTAAAGAAGTAGAAA
ATTCTATTATTTCTAATAAATCTATAAATAATATTTATATCTGA

Upstream 100 bases:

>100_bases
ATAATTTGTAGATCAAATACTATATTGATTTATAAAAATAATGTTTAATATTAAAAGTTTATAATGAATAGAAAATTAGT
CTTATATTAAGGATATTATT

Downstream 100 bases:

>100_bases
TATAATTATATTTTTAAATAGTACAAAACTTAATCTTTTTTATGTGATTCATCTATTATATCATAAATTTAGAAATTAAA
AAATTTTTATTTTTAATAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MLNPIVRKFQYGQHTITLETGIMARQATAAVMASMDDTAVFVTVVGEKTTNSSQKFFPLTVNYQERTYAVGRIPGGFFRR
EGRPSENEILTARLIDRPIRPLFPKGFCNEIQIIATVVSVNPQINPDIISIIGASAALSLSGIPFYGPIGAARVGFVNNQ
YVLNPTIDDMKSSFLDLVVSGTQNAVLMVEAESKVLSEDKILGAIMFGHQQQQVVINNIRSLSNEASKLPWIISYPEINT
ELELKITKLAEKDISNAYLIFNKQERYEKLNFLKEEIIKLFFSENSNIDISEIEDIFEKIEKNIVRKRILNNENRIDGRE
KDMIRALDIRTGVLPRTHGSSLFTRGETQSLVSVTLGTSRDAQNLDELLGDKTDNFLFHYNFPPYSVGEIGIVGSPKRRE
IGHGRLAKRSLLAVMPKLDDFPYTIRIVSEITESNGSSSMASVCGASLALMDAGVPIKSAVAGISMGLVKEGDKYVLLSD
ILGDEDHLGDMDFKVSGTEEGITALQMDIKIEGITNEIMRIALNKAKSARLHILNVMKQALSKPRNEISEFAPRIHKIKI
NPEKIKDVIGKGGSVIRMLTEETGTIIEIEDDGTIKISATIGEKAKNAIRRIEEITAEIEVGRIYSGKVTRIVDFGAFIS
IGIGKEGLVHISQISNKRVEKVSDHLTVDQIISVKVLEIDRQGRLRLSIKEVENSIISNKSINNIYI

Sequences:

>Translated_707_residues
MLNPIVRKFQYGQHTITLETGIMARQATAAVMASMDDTAVFVTVVGEKTTNSSQKFFPLTVNYQERTYAVGRIPGGFFRR
EGRPSENEILTARLIDRPIRPLFPKGFCNEIQIIATVVSVNPQINPDIISIIGASAALSLSGIPFYGPIGAARVGFVNNQ
YVLNPTIDDMKSSFLDLVVSGTQNAVLMVEAESKVLSEDKILGAIMFGHQQQQVVINNIRSLSNEASKLPWIISYPEINT
ELELKITKLAEKDISNAYLIFNKQERYEKLNFLKEEIIKLFFSENSNIDISEIEDIFEKIEKNIVRKRILNNENRIDGRE
KDMIRALDIRTGVLPRTHGSSLFTRGETQSLVSVTLGTSRDAQNLDELLGDKTDNFLFHYNFPPYSVGEIGIVGSPKRRE
IGHGRLAKRSLLAVMPKLDDFPYTIRIVSEITESNGSSSMASVCGASLALMDAGVPIKSAVAGISMGLVKEGDKYVLLSD
ILGDEDHLGDMDFKVSGTEEGITALQMDIKIEGITNEIMRIALNKAKSARLHILNVMKQALSKPRNEISEFAPRIHKIKI
NPEKIKDVIGKGGSVIRMLTEETGTIIEIEDDGTIKISATIGEKAKNAIRRIEEITAEIEVGRIYSGKVTRIVDFGAFIS
IGIGKEGLVHISQISNKRVEKVSDHLTVDQIISVKVLEIDRQGRLRLSIKEVENSIISNKSINNIYI
>Mature_707_residues
MLNPIVRKFQYGQHTITLETGIMARQATAAVMASMDDTAVFVTVVGEKTTNSSQKFFPLTVNYQERTYAVGRIPGGFFRR
EGRPSENEILTARLIDRPIRPLFPKGFCNEIQIIATVVSVNPQINPDIISIIGASAALSLSGIPFYGPIGAARVGFVNNQ
YVLNPTIDDMKSSFLDLVVSGTQNAVLMVEAESKVLSEDKILGAIMFGHQQQQVVINNIRSLSNEASKLPWIISYPEINT
ELELKITKLAEKDISNAYLIFNKQERYEKLNFLKEEIIKLFFSENSNIDISEIEDIFEKIEKNIVRKRILNNENRIDGRE
KDMIRALDIRTGVLPRTHGSSLFTRGETQSLVSVTLGTSRDAQNLDELLGDKTDNFLFHYNFPPYSVGEIGIVGSPKRRE
IGHGRLAKRSLLAVMPKLDDFPYTIRIVSEITESNGSSSMASVCGASLALMDAGVPIKSAVAGISMGLVKEGDKYVLLSD
ILGDEDHLGDMDFKVSGTEEGITALQMDIKIEGITNEIMRIALNKAKSARLHILNVMKQALSKPRNEISEFAPRIHKIKI
NPEKIKDVIGKGGSVIRMLTEETGTIIEIEDDGTIKISATIGEKAKNAIRRIEEITAEIEVGRIYSGKVTRIVDFGAFIS
IGIGKEGLVHISQISNKRVEKVSDHLTVDQIISVKVLEIDRQGRLRLSIKEVENSIISNKSINNIYI

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=38.655462184874, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=75.1085383502171, Blast_Score=1097, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=76, Percent_Identity=43.421052631579, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=687, Percent_Identity=33.0422125181951, Blast_Score=340, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17535281, Length=79, Percent_Identity=48.1012658227848, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281362905, Length=721, Percent_Identity=37.5866851595007, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=721, Percent_Identity=37.5866851595007, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=721, Percent_Identity=37.5866851595007, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=37.9204892966361, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BUCAP (Q8K9H5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660703.1
- ProteinModelPortal:   Q8K9H5
- EnsemblBacteria:   EBBUCT00000000244
- GeneID:   1005794
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg361
- GeneTree:   EBGT00050000007805
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   BAPH198804:BUSG361-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78391; Mature: 78391

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPIVRKFQYGQHTITLETGIMARQATAAVMASMDDTAVFVTVVGEKTTNSSQKFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEE
VNYQERTYAVGRIPGGFFRREGRPSENEILTARLIDRPIRPLFPKGFCNEIQIIATVVSV
EECCCCEEEEECCCCHHHHCCCCCCHHHEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEC
NPQINPDIISIIGASAALSLSGIPFYGPIGAARVGFVNNQYVLNPTIDDMKSSFLDLVVS
CCCCCCHHHHHHCCHHEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHCC
GTQNAVLMVEAESKVLSEDKILGAIMFGHQQQQVVINNIRSLSNEASKLPWIISYPEINT
CCCCEEEEEECCCHHHCCHHHEEEEEECCCCHHHHHHHHHHHHCHHHCCCEEEECCCCCC
ELELKITKLAEKDISNAYLIFNKQERYEKLNFLKEEIIKLFFSENSNIDISEIEDIFEKI
EEEEEEEEHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EKNIVRKRILNNENRIDGREKDMIRALDIRTGVLPRTHGSSLFTRGETQSLVSVTLGTSR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCCC
DAQNLDELLGDKTDNFLFHYNFPPYSVGEIGIVGSPKRREIGHGRLAKRSLLAVMPKLDD
CHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
FPYTIRIVSEITESNGSSSMASVCGASLALMDAGVPIKSAVAGISMGLVKEGDKYVLLSD
CCEEEEEEHHHHHCCCCHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEH
ILGDEDHLGDMDFKVSGTEEGITALQMDIKIEGITNEIMRIALNKAKSARLHILNVMKQA
HCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHH
LSKPRNEISEFAPRIHKIKINPEKIKDVIGKGGSVIRMLTEETGTIIEIEDDGTIKISAT
HHCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEEEEEE
IGEKAKNAIRRIEEITAEIEVGRIYSGKVTRIVDFGAFISIGIGKEGLVHISQISNKRVE
CCHHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCCEEEEECCCCCCEEHHHHCCHHHH
KVSDHLTVDQIISVKVLEIDRQGRLRLSIKEVENSIISNKSINNIYI
HHHHHCCHHHHHEEEEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLNPIVRKFQYGQHTITLETGIMARQATAAVMASMDDTAVFVTVVGEKTTNSSQKFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEE
VNYQERTYAVGRIPGGFFRREGRPSENEILTARLIDRPIRPLFPKGFCNEIQIIATVVSV
EECCCCEEEEECCCCHHHHCCCCCCHHHEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEC
NPQINPDIISIIGASAALSLSGIPFYGPIGAARVGFVNNQYVLNPTIDDMKSSFLDLVVS
CCCCCCHHHHHHCCHHEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHCC
GTQNAVLMVEAESKVLSEDKILGAIMFGHQQQQVVINNIRSLSNEASKLPWIISYPEINT
CCCCEEEEEECCCHHHCCHHHEEEEEECCCCHHHHHHHHHHHHCHHHCCCEEEECCCCCC
ELELKITKLAEKDISNAYLIFNKQERYEKLNFLKEEIIKLFFSENSNIDISEIEDIFEKI
EEEEEEEEHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EKNIVRKRILNNENRIDGREKDMIRALDIRTGVLPRTHGSSLFTRGETQSLVSVTLGTSR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCCC
DAQNLDELLGDKTDNFLFHYNFPPYSVGEIGIVGSPKRREIGHGRLAKRSLLAVMPKLDD
CHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
FPYTIRIVSEITESNGSSSMASVCGASLALMDAGVPIKSAVAGISMGLVKEGDKYVLLSD
CCEEEEEEHHHHHCCCCHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEH
ILGDEDHLGDMDFKVSGTEEGITALQMDIKIEGITNEIMRIALNKAKSARLHILNVMKQA
HCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHH
LSKPRNEISEFAPRIHKIKINPEKIKDVIGKGGSVIRMLTEETGTIIEIEDDGTIKISAT
HHCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEEEEEE
IGEKAKNAIRRIEEITAEIEVGRIYSGKVTRIVDFGAFISIGIGKEGLVHISQISNKRVE
CCHHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCCEEEEECCCCCCEEHHHHCCHHHH
KVSDHLTVDQIISVKVLEIDRQGRLRLSIKEVENSIISNKSINNIYI
HHHHHCCHHHHHEEEEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438