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The map label for this gene is ddl

Identifier: 21672494

GI number: 21672494

Start: 236288

End: 237208

Strand: Reverse

Name: ddl

Synonym: BUsg208

Alternate gene names: 21672494

Gene position: 237208-236288 (Counterclockwise)

Preceding gene: 21672495

Following gene: 21672493

Centisome position: 36.98

GC content: 26.06

Gene sequence:

>921_bases
ATGAAAAAAAAAATAGCAGTATTATTAGGAGGAAATTCTTCTGAAAGAAAAATTTCAATTAAATCAGGATATGCAATTCT
TCAAAGTTTATTAAGATCTGGATTTAACGCATATGCAATTGATACTCGTGATTTTCCTATTATGCAACTAAAAAAACAAG
GTTTTGACAGTGCTTATATTGCACTGCATGGAACAGGAGGTGAAGATGGTAGTATTCAAGGAATTCTTGAATATTTAAAT
ATTCCTTATACAGGTAGTGGAATCATGTCCTCTGCAATTTCTTTAGATAAATGGAGAACTAAATTATTGTGGAAATCTTT
ATCGCTGCGAGTATTACCAGATATATATCTACAAAAAAAAGATATTTCAAAATATACGTATTCTTATATTTTAAAAAAAA
TTTTAAAATTAAAATTTCCCGTTGTAATAAAACCAAATAATGCTGGTTCTAGTATAGGAATCACAATAGTTAATCATCCA
GATCTATTAATTGACTCTATTAATCTCGCATTCAATTATAGTAATAATATAATTATTGAAAAATTTTTAAAAGGAACGGA
ATACACAGTATCAATTCTTAATAAAAAAGTATTACCTCCGATAAAAATTATTACAAAAAATAATTTTTACGATTATAGTT
CTAAATATATAGAATCTTCGACTGAATATATATGTCCAAGTGGATTAAATTATCAAAAAGAAGAAGAGTTAAAAAAAATT
GTAGAAATAGCTTGGAATTCTTTAGGATGTAAAGGTTGCGGAAGAATCGATGCAATATTAGATAATAAAGATAAATTTTG
GTTGTTAGAAATAAACACTATTCCTGGCATGACTCATCGAAGTTTAGTTCCAATGGCAGCTAAAAGTATAGGAATATCAT
TTGATGAACTGATATTAAAAATTTTGAAAATAAATAAATAA

Upstream 100 bases:

>100_bases
CTCATTTAAACGGAAATGATATTATTTTAATACAAGGTGCGGGAGATATAGATACTATAATAAATAAAATTTTTATCTTA
AAAAATAAAAAAGTGATAAT

Downstream 100 bases:

>100_bases
ATAAAAAAAATAATTTTTATAATTATTACTAATTTTTAATTTTTTGATTAATAAAACTATTTAAAATTAAAAATTTCTTC
AATAAAGATCAGAACAGAAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKKKIAVLLGGNSSERKISIKSGYAILQSLLRSGFNAYAIDTRDFPIMQLKKQGFDSAYIALHGTGGEDGSIQGILEYLN
IPYTGSGIMSSAISLDKWRTKLLWKSLSLRVLPDIYLQKKDISKYTYSYILKKILKLKFPVVIKPNNAGSSIGITIVNHP
DLLIDSINLAFNYSNNIIIEKFLKGTEYTVSILNKKVLPPIKIITKNNFYDYSSKYIESSTEYICPSGLNYQKEEELKKI
VEIAWNSLGCKGCGRIDAILDNKDKFWLLEINTIPGMTHRSLVPMAAKSIGISFDELILKILKINK

Sequences:

>Translated_306_residues
MKKKIAVLLGGNSSERKISIKSGYAILQSLLRSGFNAYAIDTRDFPIMQLKKQGFDSAYIALHGTGGEDGSIQGILEYLN
IPYTGSGIMSSAISLDKWRTKLLWKSLSLRVLPDIYLQKKDISKYTYSYILKKILKLKFPVVIKPNNAGSSIGITIVNHP
DLLIDSINLAFNYSNNIIIEKFLKGTEYTVSILNKKVLPPIKIITKNNFYDYSSKYIESSTEYICPSGLNYQKEEELKKI
VEIAWNSLGCKGCGRIDAILDNKDKFWLLEINTIPGMTHRSLVPMAAKSIGISFDELILKILKINK
>Mature_306_residues
MKKKIAVLLGGNSSERKISIKSGYAILQSLLRSGFNAYAIDTRDFPIMQLKKQGFDSAYIALHGTGGEDGSIQGILEYLN
IPYTGSGIMSSAISLDKWRTKLLWKSLSLRVLPDIYLQKKDISKYTYSYILKKILKLKFPVVIKPNNAGSSIGITIVNHP
DLLIDSINLAFNYSNNIIIEKFLKGTEYTVSILNKKVLPPIKIITKNNFYDYSSKYIESSTEYICPSGLNYQKEEELKKI
VEIAWNSLGCKGCGRIDAILDNKDKFWLLEINTIPGMTHRSLVPMAAKSIGISFDELILKILKINK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=47.6973684210526, Blast_Score=321, Evalue=3e-89,
Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=26.8156424581006, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BUCAP (O51927)

Other databases:

- EMBL:   AF012886
- EMBL:   AE013218
- RefSeq:   NP_660561.1
- ProteinModelPortal:   O51927
- SMR:   O51927
- EnsemblBacteria:   EBBUCT00000000030
- GeneID:   1005405
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg208
- GeneTree:   EBGT00050000008200
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- ProtClustDB:   PRK01372
- BioCyc:   BAPH198804:BUSG208-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34389; Mature: 34389

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: ACT_SITE 15-15 ACT_SITE 150-150 ACT_SITE 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIAVLLGGNSSERKISIKSGYAILQSLLRSGFNAYAIDTRDFPIMQLKKQGFDSAYI
CCCEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEEE
ALHGTGGEDGSIQGILEYLNIPYTGSGIMSSAISLDKWRTKLLWKSLSLRVLPDIYLQKK
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEEHH
DISKYTYSYILKKILKLKFPVVIKPNNAGSSIGITIVNHPDLLIDSINLAFNYSNNIIIE
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEEEEEEEEEECCCCEEEE
KFLKGTEYTVSILNKKVLPPIKIITKNNFYDYSSKYIESSTEYICPSGLNYQKEEELKKI
HHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHCCCCCEECCCCCCCCHHHHHHHH
VEIAWNSLGCKGCGRIDAILDNKDKFWLLEINTIPGMTHRSLVPMAAKSIGISFDELILK
HHHHHHCCCCCCCCCEEHEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHH
ILKINK
HHHCCC
>Mature Secondary Structure
MKKKIAVLLGGNSSERKISIKSGYAILQSLLRSGFNAYAIDTRDFPIMQLKKQGFDSAYI
CCCEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEEE
ALHGTGGEDGSIQGILEYLNIPYTGSGIMSSAISLDKWRTKLLWKSLSLRVLPDIYLQKK
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEEHH
DISKYTYSYILKKILKLKFPVVIKPNNAGSSIGITIVNHPDLLIDSINLAFNYSNNIIIE
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEEEEEEEEEECCCCEEEE
KFLKGTEYTVSILNKKVLPPIKIITKNNFYDYSSKYIESSTEYICPSGLNYQKEEELKKI
HHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHCCCCCEECCCCCCCCHHHHHHHH
VEIAWNSLGCKGCGRIDAILDNKDKFWLLEINTIPGMTHRSLVPMAAKSIGISFDELILK
HHHHHHCCCCCCCCCEEHEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHH
ILKINK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9425245; 12089438