Click here to switch to the map view.

The map label for this gene is panB

Identifier: 21672478

GI number: 21672478

Start: 214671

End: 215462

Strand: Reverse

Name: panB

Synonym: BUsg191

Alternate gene names: 21672478

Gene position: 215462-214671 (Counterclockwise)

Preceding gene: 21672479

Following gene: 21672477

Centisome position: 33.59

GC content: 28.28

Gene sequence:

>792_bases
ATGGAATCTATTACTATATCAAAAATACAAAACTGGAAAAAAAATCAAATAAAATTTGCTGCTATTACAGCTTATGATTT
CAGTTTTTCTAGACTTTTTGAAAAGGAAGGTATTCCTATTATGCTTGTAGGTGATTCTCTAGGTATGACTATTCAAGGTC
ATAATTCTACACTACCTGTAAAAATTCAAGATATTAAATATCATACAAAAGCAGTGAGAAGAGGTGCACCAAATTCCTTT
TTATTGTCTGATTTACCATTTATGTCGTATTATAGTATCGAAGAAACTTTAAAAAACACAGCTAAAATTATACAATCTGG
TGCTAATATGATAAAAATAGAAGGTGGAAAATGGTTAGTTGAAACTGTCAAAGAACTATCAAAAAGATCAATATTAGTGT
GTGGTCATATAGGATTAACCCCACAATCTATAAATTTTTTAAGCGGGTATAAAATTCAAGGAAAAGAAAAAAATGATGCA
CAACGAATAATAGATGAAGCCTTTATTCTAGAAGAAGCAGGAATTAAAATGCTAGTTTTAGAATGTATTCCTTCACTTTT
AGCTAAAAAAATAACTGAAAACTTATCTATTCCAGTAATTGGTATAGGATCAGGTCATCATACTGACGGACAAATACTTG
TTATGCAAGATTTATTAGGAATAACGGATGGAAAAAAACTAAAATTTGTAAAAAATTTCCTTTGTCACAATGGAAGCATT
CAAAATGCTATTAAACAATATATCAATGAAGTTAAAAATGGAAATTTCCCCAGTGAAAAATATAGTTTTTAA

Upstream 100 bases:

>100_bases
AATATATATAGATGAGGATATTCGATTGATATATTAAATTATATCGAGTATCCTTTCTTTAGTTTTGTCAATTATGTTCT
TTAAACAAAGTAGGTAAATT

Downstream 100 bases:

>100_bases
ATTTTTGGAATGATCATGAATATTATAAAAAATATCAACCTACTAGAAAAAAAAATAAAAGAACTAAAAAAAAAGAATAA
AATAATTGGATTAGTACCAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MESITISKIQNWKKNQIKFAAITAYDFSFSRLFEKEGIPIMLVGDSLGMTIQGHNSTLPVKIQDIKYHTKAVRRGAPNSF
LLSDLPFMSYYSIEETLKNTAKIIQSGANMIKIEGGKWLVETVKELSKRSILVCGHIGLTPQSINFLSGYKIQGKEKNDA
QRIIDEAFILEEAGIKMLVLECIPSLLAKKITENLSIPVIGIGSGHHTDGQILVMQDLLGITDGKKLKFVKNFLCHNGSI
QNAIKQYINEVKNGNFPSEKYSF

Sequences:

>Translated_263_residues
MESITISKIQNWKKNQIKFAAITAYDFSFSRLFEKEGIPIMLVGDSLGMTIQGHNSTLPVKIQDIKYHTKAVRRGAPNSF
LLSDLPFMSYYSIEETLKNTAKIIQSGANMIKIEGGKWLVETVKELSKRSILVCGHIGLTPQSINFLSGYKIQGKEKNDA
QRIIDEAFILEEAGIKMLVLECIPSLLAKKITENLSIPVIGIGSGHHTDGQILVMQDLLGITDGKKLKFVKNFLCHNGSI
QNAIKQYINEVKNGNFPSEKYSF
>Mature_263_residues
MESITISKIQNWKKNQIKFAAITAYDFSFSRLFEKEGIPIMLVGDSLGMTIQGHNSTLPVKIQDIKYHTKAVRRGAPNSF
LLSDLPFMSYYSIEETLKNTAKIIQSGANMIKIEGGKWLVETVKELSKRSILVCGHIGLTPQSINFLSGYKIQGKEKNDA
QRIIDEAFILEEAGIKMLVLECIPSLLAKKITENLSIPVIGIGSGHHTDGQILVMQDLLGITDGKKLKFVKNFLCHNGSI
QNAIKQYINEVKNGNFPSEKYSF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=57.4144486692015, Blast_Score=343, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6319653, Length=262, Percent_Identity=37.0229007633588, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BUCAP (Q8K9U6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660545.1
- ProteinModelPortal:   Q8K9U6
- SMR:   Q8K9U6
- EnsemblBacteria:   EBBUCT00000000046
- GeneID:   1005388
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg191
- GeneTree:   EBGT00050000008082
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   BAPH198804:BUSG191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29353; Mature: 29353

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESITISKIQNWKKNQIKFAAITAYDFSFSRLFEKEGIPIMLVGDSLGMTIQGHNSTLPV
CCCEEHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCE
KIQDIKYHTKAVRRGAPNSFLLSDLPFMSYYSIEETLKNTAKIIQSGANMIKIEGGKWLV
EEECHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
ETVKELSKRSILVCGHIGLTPQSINFLSGYKIQGKEKNDAQRIIDEAFILEEAGIKMLVL
HHHHHHHHCCEEEEECCCCCCCCCHHHCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
ECIPSLLAKKITENLSIPVIGIGSGHHTDGQILVMQDLLGITDGKKLKFVKNFLCHNGSI
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCH
QNAIKQYINEVKNGNFPSEKYSF
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MESITISKIQNWKKNQIKFAAITAYDFSFSRLFEKEGIPIMLVGDSLGMTIQGHNSTLPV
CCCEEHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCE
KIQDIKYHTKAVRRGAPNSFLLSDLPFMSYYSIEETLKNTAKIIQSGANMIKIEGGKWLV
EEECHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
ETVKELSKRSILVCGHIGLTPQSINFLSGYKIQGKEKNDAQRIIDEAFILEEAGIKMLVL
HHHHHHHHCCEEEEECCCCCCCCCHHHCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
ECIPSLLAKKITENLSIPVIGIGSGHHTDGQILVMQDLLGITDGKKLKFVKNFLCHNGSI
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCH
QNAIKQYINEVKNGNFPSEKYSF
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438