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The map label for this gene is ribF

Identifier: 21672433

GI number: 21672433

Start: 160207

End: 161145

Strand: Reverse

Name: ribF

Synonym: BUsg143

Alternate gene names: 21672433

Gene position: 161145-160207 (Counterclockwise)

Preceding gene: 21672435

Following gene: 21672432

Centisome position: 25.12

GC content: 21.83

Gene sequence:

>939_bases
ATGAAGCTCATACGAGGCATTCATAATCTAAAAAAAATAAATTCTAATTCAGCTGTAAGTATTGGAAATTTTGATGGAGT
ACATCTAGGACATCAAAAATTACTTTCTAATTTATACAAAATAGGAAAAAAAAACAACATATTAACTGTACTTATCTTAT
TTGAACCACAACCATTAGAATTTTTAAACAATAAAAACTCTCCTAAAAGACTTACAACAATACAAAATAAAATTAAATAC
ATTCAATCATGGAAAATTGATATTATTTTATGTATTAAATTCAATGAATCTTTTTCATCTTTAAGCGCAGAAAAATTTAT
AAAAAATATTTTAATAACAAAATTGAATATAAAATTTATAATAATTGGAGATGATTTTAGATTCGGATCAAAAAGAAATG
GAAATATATCTCTCTTAAAAGAAATTGGATATCAATATAACTTTAAAGTTATAGAAATCAGTTCATTATTATATAAAAAT
AAAATTAAAATAAGCAGCACTAATATAAGAAAATGTTTATTAGAAAACAAAATTGAACTAGCAAGAAAATTACTTGGTCG
ACCATTTAGTATCTCAGGTCGTGTAATTCACGGGAATAAAATAGGACGAAAATTAGGATATCCAACTGCAAATATATCAT
TACGTAAAAATATACCTTTAAATAACGGAGTATACGCTGTAAAAATCTCCTGTTTTTTTAATAAAAAATTTGTAGGAATA
TGTAATATTGGAATTAAACCAAGTTATTTTTCTTCTAAAAAACATAGACTTCTTGAAGTCCACTTATTCAATTTTAATTT
GAATCTATATGAAGAAAAAATAGAAGTTTTTTTATACAAAAAAATAAGAAATGAATGTTTTTTTTCTTCTAAAAACAAAT
TAAAAGAACAAATTTCAAAAGATATTGAAATTGTTAAAAAATATTTTAACCTAATTTAA

Upstream 100 bases:

>100_bases
TAATATTATATATTAGTAAGAATAATACATCAAAATTATTTATAAAATAAACAAAAATTTTTAATAACAAATCACTTCAT
TTCATTAGGTCAATTTAACA

Downstream 100 bases:

>100_bases
AATTAATATCAAAAATAAAGAGAAAGTTATGAATGATTATAAAAATACTTTAAATTTACCTAAAACACAATTTTCCATGC
GAGCTAATCTATCTCAAAAA

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKLIRGIHNLKKINSNSAVSIGNFDGVHLGHQKLLSNLYKIGKKNNILTVLILFEPQPLEFLNNKNSPKRLTTIQNKIKY
IQSWKIDIILCIKFNESFSSLSAEKFIKNILITKLNIKFIIIGDDFRFGSKRNGNISLLKEIGYQYNFKVIEISSLLYKN
KIKISSTNIRKCLLENKIELARKLLGRPFSISGRVIHGNKIGRKLGYPTANISLRKNIPLNNGVYAVKISCFFNKKFVGI
CNIGIKPSYFSSKKHRLLEVHLFNFNLNLYEEKIEVFLYKKIRNECFFSSKNKLKEQISKDIEIVKKYFNLI

Sequences:

>Translated_312_residues
MKLIRGIHNLKKINSNSAVSIGNFDGVHLGHQKLLSNLYKIGKKNNILTVLILFEPQPLEFLNNKNSPKRLTTIQNKIKY
IQSWKIDIILCIKFNESFSSLSAEKFIKNILITKLNIKFIIIGDDFRFGSKRNGNISLLKEIGYQYNFKVIEISSLLYKN
KIKISSTNIRKCLLENKIELARKLLGRPFSISGRVIHGNKIGRKLGYPTANISLRKNIPLNNGVYAVKISCFFNKKFVGI
CNIGIKPSYFSSKKHRLLEVHLFNFNLNLYEEKIEVFLYKKIRNECFFSSKNKLKEQISKDIEIVKKYFNLI
>Mature_312_residues
MKLIRGIHNLKKINSNSAVSIGNFDGVHLGHQKLLSNLYKIGKKNNILTVLILFEPQPLEFLNNKNSPKRLTTIQNKIKY
IQSWKIDIILCIKFNESFSSLSAEKFIKNILITKLNIKFIIIGDDFRFGSKRNGNISLLKEIGYQYNFKVIEISSLLYKN
KIKISSTNIRKCLLENKIELARKLLGRPFSISGRVIHGNKIGRKLGYPTANISLRKNIPLNNGVYAVKISCFFNKKFVGI
CNIGIKPSYFSSKKHRLLEVHLFNFNLNLYEEKIEVFLYKKIRNECFFSSKNKLKEQISKDIEIVKKYFNLI

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family

Homologues:

Organism=Escherichia coli, GI1786208, Length=312, Percent_Identity=42.6282051282051, Blast_Score=298, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBF_BUCAP (Q8K9Z1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660500.1
- ProteinModelPortal:   Q8K9Z1
- SMR:   Q8K9Z1
- EnsemblBacteria:   EBBUCT00000000232
- GeneID:   1005960
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg143
- GeneTree:   EBGT00050000008061
- HOGENOM:   HBG465096
- OMA:   GIHNILP
- ProtClustDB:   CLSK315545
- BioCyc:   BAPH198804:BUSG143-MONOMER
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729
- Gene3D:   G3DSA:2.40.30.30
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR22749
- PIRSF:   PIRSF004491
- SMART:   SM00904
- TIGRFAMs:   TIGR00083

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase; SSF82114 Riboflavin_kinase

EC number: =2.7.1.26; =2.7.7.2

Molecular weight: Translated: 36139; Mature: 36139

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIRGIHNLKKINSNSAVSIGNFDGVHLGHQKLLSNLYKIGKKNNILTVLILFEPQPLE
CCHHHHHHHHHHCCCCCEEEECCCCCEECCHHHHHHHHHHCCCCCCEEEEEEEECCCCHH
FLNNKNSPKRLTTIQNKIKYIQSWKIDIILCIKFNESFSSLSAEKFIKNILITKLNIKFI
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEE
IIGDDFRFGSKRNGNISLLKEIGYQYNFKVIEISSLLYKNKIKISSTNIRKCLLENKIEL
EEECCCCCCCCCCCCCHHHHHCCCEEEEEEEEEEHHHHHCEEEEECCHHHHHHHHHHHHH
ARKLLGRPFSISGRVIHGNKIGRKLGYPTANISLRKNIPLNNGVYAVKISCFFNKKFVGI
HHHHHCCCCCCCCEEEECCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEECCEEEEE
CNIGIKPSYFSSKKHRLLEVHLFNFNLNLYEEKIEVFLYKKIRNECFFSSKNKLKEQISK
EECCCCCCCCCCCCCEEEEEEEEEEECEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DIEIVKKYFNLI
HHHHHHHHHHCC
>Mature Secondary Structure
MKLIRGIHNLKKINSNSAVSIGNFDGVHLGHQKLLSNLYKIGKKNNILTVLILFEPQPLE
CCHHHHHHHHHHCCCCCEEEECCCCCEECCHHHHHHHHHHCCCCCCEEEEEEEECCCCHH
FLNNKNSPKRLTTIQNKIKYIQSWKIDIILCIKFNESFSSLSAEKFIKNILITKLNIKFI
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEE
IIGDDFRFGSKRNGNISLLKEIGYQYNFKVIEISSLLYKNKIKISSTNIRKCLLENKIEL
EEECCCCCCCCCCCCCHHHHHCCCEEEEEEEEEEHHHHHCEEEEECCHHHHHHHHHHHHH
ARKLLGRPFSISGRVIHGNKIGRKLGYPTANISLRKNIPLNNGVYAVKISCFFNKKFVGI
HHHHHCCCCCCCCEEEECCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEECCEEEEE
CNIGIKPSYFSSKKHRLLEVHLFNFNLNLYEEKIEVFLYKKIRNECFFSSKNKLKEQISK
EECCCCCCCCCCCCCEEEEEEEEEEECEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DIEIVKKYFNLI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438