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The map label for this gene is carA

Identifier: 21672428

GI number: 21672428

Start: 153751

End: 154908

Strand: Reverse

Name: carA

Synonym: BUsg138

Alternate gene names: 21672428

Gene position: 154908-153751 (Counterclockwise)

Preceding gene: 21672429

Following gene: 21672427

Centisome position: 24.15

GC content: 29.88

Gene sequence:

>1158_bases
ATGGAGGATGCTTTGGGCCAATTAGCAGTACTAGTTTTAGAAGATGGAACTAGATTTCATGGACGTTCTATTGGAGCTAA
GGGGACAACTGTAGGTGAAGTTGTTTTTAATACATCAATAACTGGATATCAAGAAATCATTACTGATCCTTCCTATTCAC
ATCAAATCGTAACATTAACTCATCCTCATATCGGCAATATTGGCACTAATTGTAACGATGAAGAATCATCAAAAATTCAT
ATAAGAGGACTAATTATTCGTGATATGTCACCAATATCAAGTAACTATAGGAGCGAAAAAAGTTTTTCTTCTTACTTAAA
AGAAAACAATATTGTTGCAATATCCGATATTGATACAAGAAAATTAACAAGGATTTTACGTACTAAAGGTTCACAAAATG
GATGTATTATAGAAGACAAAAAAGAAAATTACAGTGTCGCATATCAAAAAGCAAAAAAATTTTTAAGTTTACAAGGATTA
GATTTAGCTAAAAAAGTTAGTACTAAATTTATCTATAATTGGGATAAAGGCAGCTTTTTTATCAATAAATCAAAATCTAA
ACTTGAAAAGAAAAAAAAATTTTTATTCCATATTGTCGTATATGATTTTGGAGTAAAAAGAAATATATTACGTATGCTTG
TAGATAGAGGATGCTATTTAACTGTAGTACCGGCTAAAACTGATCCCAAAATAGCTTTAAATTTAAATCCTGATGGAATT
TTTCTATCTAATGGTCCTGGTGATCCAAGACCATGCGATTATGCTATTCATGCAATTCAATGTTTCTTAAAAAAAAACAT
TCCTATTTTTGGAATTTGTTTAGGACATCAACTTCTTGCATTAGCTAGTGGAGCTAATATTATCAAAATGAAATTCGGTC
ATCATGGTGGAAATCATCCTGTAAAAGAAATTAAAACAAATCGAGTAATAATCACATCACAAAATCATAGCTTTACCGTA
GATGCAAAAAATATGCCAAATAATATTGCTATAACACATAGCTCACTTTTTGACGGAACATTACAAGGATTATGTTTAAC
TGATAAATTAGCTTTTAGTTTTCAAGGACATCCAGAAGCTAGTCCTGGACCACATGATGCTTCATCTTTATTTGATCATT
TCATTAAATTACTCAATCAAGTAAAATTCAGTAATTAA

Upstream 100 bases:

>100_bases
TTTACTACGATAATAGACAAAAATATTAAATTTAGTTAAAATAATATGCAATTTTTCATATTTTTTTAACGATTTTGTGC
TTTTATTTAAAAATTTTTAA

Downstream 100 bases:

>100_bases
TTAGGAAAAAAAGATGCCTAAATCTACTGATATAAAATCAATTTTAATTCTTGGTGCGGGACCAATAGTCATCGGACAAG
CATGTGAGTTTGATTATTCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MEDALGQLAVLVLEDGTRFHGRSIGAKGTTVGEVVFNTSITGYQEIITDPSYSHQIVTLTHPHIGNIGTNCNDEESSKIH
IRGLIIRDMSPISSNYRSEKSFSSYLKENNIVAISDIDTRKLTRILRTKGSQNGCIIEDKKENYSVAYQKAKKFLSLQGL
DLAKKVSTKFIYNWDKGSFFINKSKSKLEKKKKFLFHIVVYDFGVKRNILRMLVDRGCYLTVVPAKTDPKIALNLNPDGI
FLSNGPGDPRPCDYAIHAIQCFLKKNIPIFGICLGHQLLALASGANIIKMKFGHHGGNHPVKEIKTNRVIITSQNHSFTV
DAKNMPNNIAITHSSLFDGTLQGLCLTDKLAFSFQGHPEASPGPHDASSLFDHFIKLLNQVKFSN

Sequences:

>Translated_385_residues
MEDALGQLAVLVLEDGTRFHGRSIGAKGTTVGEVVFNTSITGYQEIITDPSYSHQIVTLTHPHIGNIGTNCNDEESSKIH
IRGLIIRDMSPISSNYRSEKSFSSYLKENNIVAISDIDTRKLTRILRTKGSQNGCIIEDKKENYSVAYQKAKKFLSLQGL
DLAKKVSTKFIYNWDKGSFFINKSKSKLEKKKKFLFHIVVYDFGVKRNILRMLVDRGCYLTVVPAKTDPKIALNLNPDGI
FLSNGPGDPRPCDYAIHAIQCFLKKNIPIFGICLGHQLLALASGANIIKMKFGHHGGNHPVKEIKTNRVIITSQNHSFTV
DAKNMPNNIAITHSSLFDGTLQGLCLTDKLAFSFQGHPEASPGPHDASSLFDHFIKLLNQVKFSN
>Mature_385_residues
MEDALGQLAVLVLEDGTRFHGRSIGAKGTTVGEVVFNTSITGYQEIITDPSYSHQIVTLTHPHIGNIGTNCNDEESSKIH
IRGLIIRDMSPISSNYRSEKSFSSYLKENNIVAISDIDTRKLTRILRTKGSQNGCIIEDKKENYSVAYQKAKKFLSLQGL
DLAKKVSTKFIYNWDKGSFFINKSKSKLEKKKKFLFHIVVYDFGVKRNILRMLVDRGCYLTVVPAKTDPKIALNLNPDGI
FLSNGPGDPRPCDYAIHAIQCFLKKNIPIFGICLGHQLLALASGANIIKMKFGHHGGNHPVKEIKTNRVIITSQNHSFTV
DAKNMPNNIAITHSSLFDGTLQGLCLTDKLAFSFQGHPEASPGPHDASSLFDHFIKLLNQVKFSN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=390, Percent_Identity=35.6410256410256, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI169790915, Length=389, Percent_Identity=35.2185089974293, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI21361331, Length=389, Percent_Identity=35.2185089974293, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=66.0427807486631, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI193204318, Length=389, Percent_Identity=33.6760925449871, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=410, Percent_Identity=34.6341463414634, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=36.6492146596859, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=34.9614395886889, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=34.9614395886889, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_BUCAP (Q8K9Z6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660495.1
- ProteinModelPortal:   Q8K9Z6
- SMR:   Q8K9Z6
- EnsemblBacteria:   EBBUCT00000000152
- GeneID:   1005955
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg138
- GeneTree:   EBGT00050000008117
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   BAPH198804:BUSG138-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42791; Mature: 42791

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 273-273 ACT_SITE 357-357 ACT_SITE 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDALGQLAVLVLEDGTRFHGRSIGAKGTTVGEVVFNTSITGYQEIITDPSYSHQIVTLT
CCCCCCEEEEEEEECCCEECCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEEE
HPHIGNIGTNCNDEESSKIHIRGLIIRDMSPISSNYRSEKSFSSYLKENNIVAISDIDTR
CCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHCCCCHHHHHHHHHCCCEEEEECCCHH
KLTRILRTKGSQNGCIIEDKKENYSVAYQKAKKFLSLQGLDLAKKVSTKFIYNWDKGSFF
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCEE
INKSKSKLEKKKKFLFHIVVYDFGVKRNILRMLVDRGCYLTVVPAKTDPKIALNLNPDGI
EECCHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCEE
FLSNGPGDPRPCDYAIHAIQCFLKKNIPIFGICLGHQLLALASGANIIKMKFGHHGGNHP
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCC
VKEIKTNRVIITSQNHSFTVDAKNMPNNIAITHSSLFDGTLQGLCLTDKLAFSFQGHPEA
HHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECHHCCCCCCCEEEEEEEEEEECCCCCC
SPGPHDASSLFDHFIKLLNQVKFSN
CCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEDALGQLAVLVLEDGTRFHGRSIGAKGTTVGEVVFNTSITGYQEIITDPSYSHQIVTLT
CCCCCCEEEEEEEECCCEECCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEEE
HPHIGNIGTNCNDEESSKIHIRGLIIRDMSPISSNYRSEKSFSSYLKENNIVAISDIDTR
CCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHCCCCHHHHHHHHHCCCEEEEECCCHH
KLTRILRTKGSQNGCIIEDKKENYSVAYQKAKKFLSLQGLDLAKKVSTKFIYNWDKGSFF
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCEE
INKSKSKLEKKKKFLFHIVVYDFGVKRNILRMLVDRGCYLTVVPAKTDPKIALNLNPDGI
EECCHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCEE
FLSNGPGDPRPCDYAIHAIQCFLKKNIPIFGICLGHQLLALASGANIIKMKFGHHGGNHP
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCC
VKEIKTNRVIITSQNHSFTVDAKNMPNNIAITHSSLFDGTLQGLCLTDKLAFSFQGHPEA
HHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECHHCCCCCCCEEEEEEEEEEECCCCCC
SPGPHDASSLFDHFIKLLNQVKFSN
CCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438