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The map label for this gene is folA

Identifier: 21672426

GI number: 21672426

Start: 149938

End: 150423

Strand: Reverse

Name: folA

Synonym: BUsg136

Alternate gene names: 21672426

Gene position: 150423-149938 (Counterclockwise)

Preceding gene: 21672427

Following gene: 21672422

Centisome position: 23.45

GC content: 24.9

Gene sequence:

>486_bases
ATGAAAATTAGTTTAATTTCAGCAATTTCAAATAATTTAGTAATTGGACATAATAATAAAATACCTTGGTATCTTCCAGA
AGATCTAAAATGGTTCAAAAAAAATACAATACATAAAGATATTATTATGGGACGTCTCACATGGGAATCTATTTTAAATC
ATCCCTTACCAATGCGAAAAAATATTGTAATCAGTCATAAAGAAATCATACATAAGGATGTGATATGGGCCAATTCAATC
TCGAAAGCATTGCTTTCTACTACATATGACAAAGAAATTATGGTAATCGGAGGATCAAAAATATATAAACAAATGTTGTT
TTATGCAACTAAGCTATATTTAACACATGTAGATTTTAATGGAATTGGTGATACTTATTTTCCTCAATATAAATCATGTA
AATTTTGGAAAACTATATTTAGAAAACAACATTTTAAAGATAAAAATAATCCTTATAATTTTTGTTTTGAAATTTTATCA
AGATAA

Upstream 100 bases:

>100_bases
AAATAAATTTAATTAATTAAAAAAATTAAATTTATAACTTTTTATAAATATGTTGTAAAACATATTTATAATTTTAAATT
TTAAAAAATCGTAAATTAAG

Downstream 100 bases:

>100_bases
AAAAATATATTAATTAAATATTTTTAATTTAATTTTAACTATTGTTTTTTTGAAAAGATTGAAAAAAGTATTTTTTATCT
TCCCATCGAAACATGGTTAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRKNIVISHKEIIHKDVIWANSI
SKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFNGIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNFCFEILS
R

Sequences:

>Translated_161_residues
MKISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRKNIVISHKEIIHKDVIWANSI
SKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFNGIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNFCFEILS
R
>Mature_161_residues
MKISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRKNIVISHKEIIHKDVIWANSI
SKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFNGIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNFCFEILS
R

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=48.4276729559748, Blast_Score=160, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324810, Length=124, Percent_Identity=30.6451612903226, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_BUCAP (Q8K9Z8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660493.1
- ProteinModelPortal:   Q8K9Z8
- SMR:   Q8K9Z8
- EnsemblBacteria:   EBBUCT00000000175
- GeneID:   1005953
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg136
- GeneTree:   EBGT00050000007986
- HOGENOM:   HBG665708
- OMA:   THIDINI
- ProtClustDB:   CLSK315509
- BioCyc:   BAPH198804:BUSG136-MONOMER
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 19114; Mature: 19114

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 28-28 BINDING 59-59 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRK
CCEEEEECCCCCEEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCCC
NIVISHKEIIHKDVIWANSISKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFN
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCC
GIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNFCFEILSR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRK
CCEEEEECCCCCEEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCCC
NIVISHKEIIHKDVIWANSISKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFN
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCC
GIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNFCFEILSR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438