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The map label for this gene is rfaE [C]

Identifier: 21672350

GI number: 21672350

Start: 65253

End: 66197

Strand: Reverse

Name: rfaE [C]

Synonym: BUsg057

Alternate gene names: 21672350

Gene position: 66197-65253 (Counterclockwise)

Preceding gene: 21672352

Following gene: 21672349

Centisome position: 10.32

GC content: 26.56

Gene sequence:

>945_bases
ATGAAAAAAAAATTAATTAATTTTAAAAACGCACTTGTTCTTGTTGTTGGAGATCTTATATTAGATTGCTACTGGTACAG
TAAAAATTACTATGTAGTATTAGAAGAATCACTACCAATTGCATCAATTAACAAAATAAAAAAACAACCAGGTGGAGCAG
CAAACGTTGCTAAAAATATCGCTGAAATTGGAGGAAATTCTAAAATTATTGGTTTTATTGGTATGGATTATGAAGGGTTA
ACATTAAAAAAACTTCTTAATCATACCAGAATTTATCCTGATTTAATCAGTATAAAAAAAAATAAAACAATCACAAAAAT
TAGAATTTTATCAGAAAAAAAACAATTAATTAGATTAGATTTTCAAGAAAAATATATTTCAAAAAAATTTAATTTATTAT
ATGAAAAAATTATTAGTTCATTAACGTACTATAAAGTTTTAGTACTTTCGGATTATGCAAAAGGCACATTAGTTAATGTA
AAAAAAATTATCACTTTAGCAAAAAAAATGTCTATTCCTATACTTATAGATCCAAAGGGATTAGATTTTAAAAAATATTC
CGGTGCAACTTTATTAACACCAAATCTTTCTGAATTTGAACAAATAGTAGGTAAATGTCATCAAGAAAAACAAATATTAC
AACGAGGAATTAAATTAGTATCTGAATTAAATCTATCGGCATTATTAGTCACTCGTTCGAAAAATGGTATGACTTTATTT
CAACCTGAAAAACAACCTATACATTTTCCAGCTTTATCTAAAACAGCATCGGACGTAACAGGTGCAGGAGATACTGTAAT
TTCAATAATAGCTTCTTCTTTAGCTACAGGATATTCGTTAGAAGAAGCTTGCTTCTACGCAAATGTAGGAGCCAGTATAG
TAATACAAAAGATAGGTACTGAAACACTAACAATCAATCAATTAAATACAGTCTTAAACATTTAA

Upstream 100 bases:

>100_bases
AGAATTTTGTAAAATAATTAAAAAAATACTCATTTTTTTAAGACACTATATATTTTTAAAAATAAAAATTAATAATTTCA
TATAATTTAAAGAGAATTTC

Downstream 100 bases:

>100_bases
TAATATATTTTTTAAATTAATAAAATTTAATTAAATTTTATTAAATTTTTTTTATATTAAAGAACAATTAAAAAATTTTT
AAAATACTTTTATATCTAAA

Product: ADP-heptose synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKKLINFKNALVLVVGDLILDCYWYSKNYYVVLEESLPIASINKIKKQPGGAANVAKNIAEIGGNSKIIGFIGMDYEGL
TLKKLLNHTRIYPDLISIKKNKTITKIRILSEKKQLIRLDFQEKYISKKFNLLYEKIISSLTYYKVLVLSDYAKGTLVNV
KKIITLAKKMSIPILIDPKGLDFKKYSGATLLTPNLSEFEQIVGKCHQEKQILQRGIKLVSELNLSALLVTRSKNGMTLF
QPEKQPIHFPALSKTASDVTGAGDTVISIIASSLATGYSLEEACFYANVGASIVIQKIGTETLTINQLNTVLNI

Sequences:

>Translated_314_residues
MKKKLINFKNALVLVVGDLILDCYWYSKNYYVVLEESLPIASINKIKKQPGGAANVAKNIAEIGGNSKIIGFIGMDYEGL
TLKKLLNHTRIYPDLISIKKNKTITKIRILSEKKQLIRLDFQEKYISKKFNLLYEKIISSLTYYKVLVLSDYAKGTLVNV
KKIITLAKKMSIPILIDPKGLDFKKYSGATLLTPNLSEFEQIVGKCHQEKQILQRGIKLVSELNLSALLVTRSKNGMTLF
QPEKQPIHFPALSKTASDVTGAGDTVISIIASSLATGYSLEEACFYANVGASIVIQKIGTETLTINQLNTVLNI
>Mature_314_residues
MKKKLINFKNALVLVVGDLILDCYWYSKNYYVVLEESLPIASINKIKKQPGGAANVAKNIAEIGGNSKIIGFIGMDYEGL
TLKKLLNHTRIYPDLISIKKNKTITKIRILSEKKQLIRLDFQEKYISKKFNLLYEKIISSLTYYKVLVLSDYAKGTLVNV
KKIITLAKKMSIPILIDPKGLDFKKYSGATLLTPNLSEFEQIVGKCHQEKQILQRGIKLVSELNLSALLVTRSKNGMTLF
QPEKQPIHFPALSKTASDVTGAGDTVISIIASSLATGYSLEEACFYANVGASIVIQKIGTETLTINQLNTVLNI

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1789432, Length=312, Percent_Identity=49.6794871794872, Blast_Score=337, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y057_BUCAP (Q8KA54)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660417.1
- ProteinModelPortal:   Q8KA54
- EnsemblBacteria:   EBBUCT00000000502
- GeneID:   1005873
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg057
- GeneTree:   EBGT00050000007797
- HOGENOM:   HBG545535
- OMA:   VIGRQQQ
- ProtClustDB:   CLSK315098
- BioCyc:   BAPH198804:BUSG057-MONOMER
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR011913
- TIGRFAMs:   TIGR02198

Pfam domain/function: PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 34921; Mature: 34921

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLINFKNALVLVVGDLILDCYWYSKNYYVVLEESLPIASINKIKKQPGGAANVAKNI
CCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHH
AEIGGNSKIIGFIGMDYEGLTLKKLLNHTRIYPDLISIKKNKTITKIRILSEKKQLIRLD
HHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHH
FQEKYISKKFNLLYEKIISSLTYYKVLVLSDYAKGTLVNVKKIITLAKKMSIPILIDPKG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
LDFKKYSGATLLTPNLSEFEQIVGKCHQEKQILQRGIKLVSELNLSALLVTRSKNGMTLF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEE
QPEKQPIHFPALSKTASDVTGAGDTVISIIASSLATGYSLEEACFYANVGASIVIQKIGT
CCCCCCEECCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCCHHEEEEECCC
ETLTINQLNTVLNI
CEEEEEHHHHHHCC
>Mature Secondary Structure
MKKKLINFKNALVLVVGDLILDCYWYSKNYYVVLEESLPIASINKIKKQPGGAANVAKNI
CCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHH
AEIGGNSKIIGFIGMDYEGLTLKKLLNHTRIYPDLISIKKNKTITKIRILSEKKQLIRLD
HHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHH
FQEKYISKKFNLLYEKIISSLTYYKVLVLSDYAKGTLVNVKKIITLAKKMSIPILIDPKG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
LDFKKYSGATLLTPNLSEFEQIVGKCHQEKQILQRGIKLVSELNLSALLVTRSKNGMTLF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEE
QPEKQPIHFPALSKTASDVTGAGDTVISIIASSLATGYSLEEACFYANVGASIVIQKIGT
CCCCCCEECCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCCHHEEEEECCC
ETLTINQLNTVLNI
CEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438