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The map label for this gene is atpF

Identifier: 21672298

GI number: 21672298

Start: 3237

End: 3728

Strand: Direct

Name: atpF

Synonym: BUsg004

Alternate gene names: 21672298

Gene position: 3237-3728 (Clockwise)

Preceding gene: 21672297

Following gene: 21672299

Centisome position: 0.5

GC content: 22.36

Gene sequence:

>492_bases
GTGAATCTTAATGCAACAATTCTTGGACAAGCACTGTCATTTATTTTATTTGTTTGGTTTTGTATGAAATATATATGGCC
TCCTATTATTTTTGCCATCGAGACAAGACAAAAAAATATTGAGGAATCTTTGATTTCTTTGAAAAAAGCTGAAGAAGAAC
TTATTATTATTCAAAAAAAAATGAATCAAATAATTCAAGATTCTAAAGAAAAAGCATCTTTTATTATCAACGAAGCAAAT
AAAAAAAAATCAATTATTTTAGAGGATGCAAAGAGTATTGCTTTAGAGGAAAGCAAAAAAATTTTTTTAAGAAATCAATT
AGAAATTGATTTAAAAGTTATGCAAGTACGTAAAAATTTACATAAAGAAATTGTAGATTTATCAATTTTGATAGCTGAAA
AAATTATTAAAGACAATATTCAAAAAGATCAATATAAGTATTCAATAAAAAAATTAATTGTTTCTTTATCTAAAGATAAG
AAATTAATATAG

Upstream 100 bases:

>100_bases
TTTTGTAAAAAAATTATTATTTTAAATTATAATTAAAATCTTTACGTTTTTTAGACAGCGTAAAGATTAATTTTTATTTT
AATACATAAGGTCCATTATC

Downstream 100 bases:

>100_bases
GTAAAATATTAATGTCAGTACTTGATACTATTGCTAGACCTTATGCTAAAGCAATTTTTGAATTAGCAATTGAAAATCAA
TCAATAGAAAAATGGAAAAA

Product: F0F1 ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: ATP synthase F(0) sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MNLNATILGQALSFILFVWFCMKYIWPPIIFAIETRQKNIEESLISLKKAEEELIIIQKKMNQIIQDSKEKASFIINEAN
KKKSIILEDAKSIALEESKKIFLRNQLEIDLKVMQVRKNLHKEIVDLSILIAEKIIKDNIQKDQYKYSIKKLIVSLSKDK
KLI

Sequences:

>Translated_163_residues
MNLNATILGQALSFILFVWFCMKYIWPPIIFAIETRQKNIEESLISLKKAEEELIIIQKKMNQIIQDSKEKASFIINEAN
KKKSIILEDAKSIALEESKKIFLRNQLEIDLKVMQVRKNLHKEIVDLSILIAEKIIKDNIQKDQYKYSIKKLIVSLSKDK
KLI
>Mature_163_residues
MNLNATILGQALSFILFVWFCMKYIWPPIIFAIETRQKNIEESLISLKKAEEELIIIQKKMNQIIQDSKEKASFIINEAN
KKKSIILEDAKSIALEESKKIFLRNQLEIDLKVMQVRKNLHKEIVDLSILIAEKIIKDNIQKDQYKYSIKKLIVSLSKDK
KLI

Specific function: Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0)

COG id: COG0711

COG function: function code C; F0F1-type ATP synthase, subunit b

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase B chain family

Homologues:

Organism=Escherichia coli, GI1790174, Length=156, Percent_Identity=41.6666666666667, Blast_Score=129, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATPF_BUCAP (O51876)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660365.1
- ProteinModelPortal:   O51876
- SMR:   O51876
- EnsemblBacteria:   EBBUCT00000000075
- GeneID:   1005824
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg004
- GeneTree:   EBGT00050000007813
- HOGENOM:   HBG617328
- OMA:   ANATRQA
- ProtClustDB:   PRK05759
- BioCyc:   BAPH198804:BUSG004-MONOMER
- HAMAP:   MF_01398
- InterPro:   IPR002146
- InterPro:   IPR005864
- TIGRFAMs:   TIGR01144

Pfam domain/function: PF00430 ATP-synt_B

EC number: 3.6.3.14

Molecular weight: Translated: 19075; Mature: 19075

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xae009e8)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNATILGQALSFILFVWFCMKYIWPPIIFAIETRQKNIEESLISLKKAEEELIIIQKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNQIIQDSKEKASFIINEANKKKSIILEDAKSIALEESKKIFLRNQLEIDLKVMQVRKNL
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKEIVDLSILIAEKIIKDNIQKDQYKYSIKKLIVSLSKDKKLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MNLNATILGQALSFILFVWFCMKYIWPPIIFAIETRQKNIEESLISLKKAEEELIIIQKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNQIIQDSKEKASFIINEANKKKSIILEDAKSIALEESKKIFLRNQLEIDLKVMQVRKNL
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKEIVDLSILIAEKIIKDNIQKDQYKYSIKKLIVSLSKDKKLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Borate; diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines analogs; Oligomycin; Quercetin; Trialkyl tin derivatives; Venturicidin [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9216881; 9516544; 12089438