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The map label for this gene is truA [H]

Identifier: 213693060

GI number: 213693060

Start: 2451750

End: 2452667

Strand: Direct

Name: truA [H]

Synonym: Blon_2209

Alternate gene names: 213693060

Gene position: 2451750-2452667 (Clockwise)

Preceding gene: 213693053

Following gene: 213693090

Centisome position: 86.55

GC content: 60.35

Gene sequence:

>918_bases
ATGGCTATGACACGACTGCGCATTGACTTGGCCTACGACGGCGGCGGATTCTATGGCTGGGCCAAACAGCCCAATCTGCG
TACGGTGCAAGGCACCATTGAAGATGCGCTGCACAAGGTGCTGCGGGTGCCGGCCGATGACGCGGCCGAACCGTTACGCT
TGACGGTGGCTGGGCGCACCGACACCGGTGTGCACGCCTCCCACCAAGTGGCGCATCTGGATGTATCCGATGAGGTACTG
AATCGCTGCGTAGGTCACATGACCATTCCGGTCACCGAGGCGCTGACTCGCAGACTCAAGGCTGTGCTGCCTTCGGACAT
CGTCATTCATGGCATTGCCGTGGCACCGGCCGGTTTCGATGCCAGGTTCTCCGCATTGGAGCGCACTTATGTGTATCGGG
TCGCCGATCGTTCGTCCGAGATCGACCCGCGCCTGCGTGGATGCGTGCTTACCGTGGATGAATCGCTGGATCTGGAGCTT
ATGAACCGCGCAGCCGCATTGACCATCGGCCTGCACGATTTTGGTTCCTTCGCCACTCCGAATCCGGGAGGCACTACGAT
CCGCGAAGTCAAGACCGCCTATTGGCGGCGGGTGCCGATCACTCCCCTGGTGCCGGATGCGATGGCATCGCATGAAGCAT
ATCGGACTCCGTCGCTGGAGTCCGGCCTGGTAGTGTTCACGATTGTGGCGGATGCCTTTGCCCGTAATATGGTGCGGTCC
CTGGTCGGCTCATGTATCAAAGTTGGTTCGGGACGCAAATCACTGGAGTGGTTTGCCGGTAAAATGGCCGACCCAGTGCG
TGAAGGCTCAAGCGGGCCGATTGCGCCGCAGGGCCTGACTTTGGAGCATATCGCATATCCCGCCGATGATCAGCTGGCCG
CAAGAGCCGAGGCCATTCGAGCCGTACGTACGTTGTAG

Upstream 100 bases:

>100_bases
GTTTCAGCAAACGCGCGAGAATGTTACAGGCAGGTCACTTCGCCGACCGCACTCGTCTCGCCGACCGCGCGCATCTCGCC
GACCGCACTCGTTACAATGA

Downstream 100 bases:

>100_bases
ATTCTATGAAACAATGCAAAAGGGCTGGAACCCAAACGGATTCCAGCCCTTTCAGCTAGCTTGGCGAAAGCTATTCAGCT
CACTCGGCCTTCTCGGCGTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAMTRLRIDLAYDGGGFYGWAKQPNLRTVQGTIEDALHKVLRVPADDAAEPLRLTVAGRTDTGVHASHQVAHLDVSDEVL
NRCVGHMTIPVTEALTRRLKAVLPSDIVIHGIAVAPAGFDARFSALERTYVYRVADRSSEIDPRLRGCVLTVDESLDLEL
MNRAAALTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDAMASHEAYRTPSLESGLVVFTIVADAFARNMVRS
LVGSCIKVGSGRKSLEWFAGKMADPVREGSSGPIAPQGLTLEHIAYPADDQLAARAEAIRAVRTL

Sequences:

>Translated_305_residues
MAMTRLRIDLAYDGGGFYGWAKQPNLRTVQGTIEDALHKVLRVPADDAAEPLRLTVAGRTDTGVHASHQVAHLDVSDEVL
NRCVGHMTIPVTEALTRRLKAVLPSDIVIHGIAVAPAGFDARFSALERTYVYRVADRSSEIDPRLRGCVLTVDESLDLEL
MNRAAALTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDAMASHEAYRTPSLESGLVVFTIVADAFARNMVRS
LVGSCIKVGSGRKSLEWFAGKMADPVREGSSGPIAPQGLTLEHIAYPADDQLAARAEAIRAVRTL
>Mature_304_residues
AMTRLRIDLAYDGGGFYGWAKQPNLRTVQGTIEDALHKVLRVPADDAAEPLRLTVAGRTDTGVHASHQVAHLDVSDEVLN
RCVGHMTIPVTEALTRRLKAVLPSDIVIHGIAVAPAGFDARFSALERTYVYRVADRSSEIDPRLRGCVLTVDESLDLELM
NRAAALTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDAMASHEAYRTPSLESGLVVFTIVADAFARNMVRSL
VGSCIKVGSGRKSLEWFAGKMADPVREGSSGPIAPQGLTLEHIAYPADDQLAARAEAIRAVRTL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=230, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI199559785, Length=277, Percent_Identity=27.4368231046931, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788657, Length=288, Percent_Identity=31.9444444444444, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321112, Length=256, Percent_Identity=28.125, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007752, Length=293, Percent_Identity=25.5972696245734, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33016; Mature: 32885

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTRLRIDLAYDGGGFYGWAKQPNLRTVQGTIEDALHKVLRVPADDAAEPLRLTVAGRT
CCCEEEEEEEEECCCCEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
DTGVHASHQVAHLDVSDEVLNRCVGHMTIPVTEALTRRLKAVLPSDIVIHGIAVAPAGFD
CCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ARFSALERTYVYRVADRSSEIDPRLRGCVLTVDESLDLELMNRAAALTIGLHDFGSFATP
HHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHCHHEEEEEHHHCCCCCCC
NPGGTTIREVKTAYWRRVPITPLVPDAMASHEAYRTPSLESGLVVFTIVADAFARNMVRS
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
LVGSCIKVGSGRKSLEWFAGKMADPVREGSSGPIAPQGLTLEHIAYPADDQLAARAEAIR
HHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
AVRTL
HHHCC
>Mature Secondary Structure 
AMTRLRIDLAYDGGGFYGWAKQPNLRTVQGTIEDALHKVLRVPADDAAEPLRLTVAGRT
CCEEEEEEEEECCCCEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
DTGVHASHQVAHLDVSDEVLNRCVGHMTIPVTEALTRRLKAVLPSDIVIHGIAVAPAGFD
CCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ARFSALERTYVYRVADRSSEIDPRLRGCVLTVDESLDLELMNRAAALTIGLHDFGSFATP
HHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHCHHEEEEEHHHCCCCCCC
NPGGTTIREVKTAYWRRVPITPLVPDAMASHEAYRTPSLESGLVVFTIVADAFARNMVRS
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
LVGSCIKVGSGRKSLEWFAGKMADPVREGSSGPIAPQGLTLEHIAYPADDQLAARAEAIR
HHHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
AVRTL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA