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The map label for this gene is metK [H]

Identifier: 213692989

GI number: 213692989

Start: 2361481

End: 2362701

Strand: Direct

Name: metK [H]

Synonym: Blon_2136

Alternate gene names: 213692989

Gene position: 2361481-2362701 (Clockwise)

Preceding gene: 213692988

Following gene: 213692990

Centisome position: 83.36

GC content: 62.74

Gene sequence:

>1221_bases
ATGACAGAAGAACATCGGCTTATCTCCGCCGAATCCGTGACCGAAGGCCACCCTGACAAGGTCTGCGATCAAATCTCCGA
CGCCATTCTTGATGATCTGCTGGTCCAGGATCCGTCCAGCCATGTGGCCGTGGAGACCTCCGCCGCCACCGGCGTGTTCC
TGGTCTTCGGCGAAGTCACGTCCAAGGGGTACTGCGACGTGCAGTCCAAGGTCCGCGAAACCCTGCGCAACATCGGCTAT
ACCTCTTCCGAAGTCGGCCTTGACGCCGATTCCTGCGGCGTGGTCGTGGCCATCACCGAGCAGAGCGCCGAAATCAACCA
GGGCGTTGCCCGTCTGACTGGCGATCAGGAGACCGCCGCCAGCCGCGAAGAACGTTACGAAGCACAGGGTGCCGGCGATC
AGGGCGTGATGTTCGGCTACGCCACCGACGAGACCCCGACGCTGATGCCGCTTCCGATCTACTTGGCACATCGCCTCGCC
TTCCGTCTGACCGAGGTGCGCAAGTCCGGTGAAGTGCCGCACCTGCGCCCGGACGGCAAGACCCAGGTAACCATTGAGTA
TGACGATAACGATAAGCCCGTTCGTCTGGACACCGTGCTGATCTCCACCCAGCATGATCCTGAGGTTACGCAGGATTGGC
TTGCTGTTGAGCTGAAGAAGCATGTGATTGACCCGGTGCTTGATGAGGTGCTGGGTTCCAAGGTGCCGCATGACAACTAC
CGCCAGCTGGTCAACCCGACCGGTTCGTTCATCCTGGGCGGCCCGGCGGCCGACGCCGGTCTGACCGGCCGCAAGATCAT
CGTCGACACCTACGGTGGCGCTGCCCACCACGGCGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGATCGTTCCG
CCGCGTACGCCACCCGTTGGGTTGCGAAGAACATCGTGGCTGCCGGCCTGGCCCACAAAGTCGAGATCCAAATCGCCTAC
GCTATTGGTGTTGCCGACCCGGTATCCGTCAACGTAGAGACCTTCGGCACCGAGCAAGGCGTGACGCGCGGCCAGATCGC
CGCCGCCGTACGCAAGGTCTTCGACCTGCGCCCCGCCGCCATCATCGACGAGCTCGACCTGAAGCGCCCGATCTACTTGA
AGACCGCCGCGTATGGCCACTTCGGACGGACCGACGTCGAGTTCCCGTGGGAAAAGACCGACAAGGTCGAGGAGCTCAAG
GCCGCGATTGCTGCGGAGTGA

Upstream 100 bases:

>100_bases
CCTTTATTTATGCCCGCTTCCGCAGTGACAAGTAAAAATTGACACCGTATCATTGTGCCTCGCATCCCGCGGGGCGATAA
GTCCTGAAGAAAGGGATGAT

Downstream 100 bases:

>100_bases
GGTGTTCTAATTAGGGCAGAGAGACCATGAGAATAGTGGGCTTTCCGTGCAATCCACTATGACGAGCCGCTCCTCGTGTT
CGCCGCCTCGAAGGTGAGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTEEHRLISAESVTEGHPDKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVTSKGYCDVQSKVRETLRNIGY
TSSEVGLDADSCGVVVAITEQSAEINQGVARLTGDQETAASREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLA
FRLTEVRKSGEVPHLRPDGKTQVTIEYDDNDKPVRLDTVLISTQHDPEVTQDWLAVELKKHVIDPVLDEVLGSKVPHDNY
RQLVNPTGSFILGGPAADAGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYATRWVAKNIVAAGLAHKVEIQIAY
AIGVADPVSVNVETFGTEQGVTRGQIAAAVRKVFDLRPAAIIDELDLKRPIYLKTAAYGHFGRTDVEFPWEKTDKVEELK
AAIAAE

Sequences:

>Translated_406_residues
MTEEHRLISAESVTEGHPDKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVTSKGYCDVQSKVRETLRNIGY
TSSEVGLDADSCGVVVAITEQSAEINQGVARLTGDQETAASREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLA
FRLTEVRKSGEVPHLRPDGKTQVTIEYDDNDKPVRLDTVLISTQHDPEVTQDWLAVELKKHVIDPVLDEVLGSKVPHDNY
RQLVNPTGSFILGGPAADAGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYATRWVAKNIVAAGLAHKVEIQIAY
AIGVADPVSVNVETFGTEQGVTRGQIAAAVRKVFDLRPAAIIDELDLKRPIYLKTAAYGHFGRTDVEFPWEKTDKVEELK
AAIAAE
>Mature_405_residues
TEEHRLISAESVTEGHPDKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVTSKGYCDVQSKVRETLRNIGYT
SSEVGLDADSCGVVVAITEQSAEINQGVARLTGDQETAASREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLAF
RLTEVRKSGEVPHLRPDGKTQVTIEYDDNDKPVRLDTVLISTQHDPEVTQDWLAVELKKHVIDPVLDEVLGSKVPHDNYR
QLVNPTGSFILGGPAADAGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYATRWVAKNIVAAGLAHKVEIQIAYA
IGVADPVSVNVETFGTEQGVTRGQIAAAVRKVFDLRPAAIIDELDLKRPIYLKTAAYGHFGRTDVEFPWEKTDKVEELKA
AIAAE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=399, Percent_Identity=52.1303258145363, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI5174529, Length=394, Percent_Identity=52.5380710659898, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=57.4307304785894, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=395, Percent_Identity=51.6455696202532, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=401, Percent_Identity=51.6209476309227, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538498, Length=395, Percent_Identity=51.8987341772152, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=51.1568123393316, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=395, Percent_Identity=47.3417721518987, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=350, Percent_Identity=50.5714285714286, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=50.8720930232558, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI32565903, Length=317, Percent_Identity=51.7350157728707, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=50.5747126436782, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320710, Length=394, Percent_Identity=52.2842639593909, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=52.6992287917738, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45552159, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580529, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580537, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580533, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580535, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580531, Length=394, Percent_Identity=49.238578680203, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=49.238578680203, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=49.238578680203, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580541, Length=396, Percent_Identity=48.2323232323232, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=51.5625, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43831; Mature: 43699

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEHRLISAESVTEGHPDKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
SKGYCDVQSKVRETLRNIGYTSSEVGLDADSCGVVVAITEQSAEINQGVARLTGDQETAA
CCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHH
SREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLAFRLTEVRKSGEVPHLRPDGK
HHHHHHHHCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQVTIEYDDNDKPVRLDTVLISTQHDPEVTQDWLAVELKKHVIDPVLDEVLGSKVPHDNY
EEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQLVNPTGSFILGGPAADAGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYATRW
HHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VAKNIVAAGLAHKVEIQIAYAIGVADPVSVNVETFGTEQGVTRGQIAAAVRKVFDLRPAA
HHHHHHHHCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHH
IIDELDLKRPIYLKTAAYGHFGRTDVEFPWEKTDKVEELKAAIAAE
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TEEHRLISAESVTEGHPDKVCDQISDAILDDLLVQDPSSHVAVETSAATGVFLVFGEVT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
SKGYCDVQSKVRETLRNIGYTSSEVGLDADSCGVVVAITEQSAEINQGVARLTGDQETAA
CCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHH
SREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLAFRLTEVRKSGEVPHLRPDGK
HHHHHHHHCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQVTIEYDDNDKPVRLDTVLISTQHDPEVTQDWLAVELKKHVIDPVLDEVLGSKVPHDNY
EEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RQLVNPTGSFILGGPAADAGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYATRW
HHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VAKNIVAAGLAHKVEIQIAYAIGVADPVSVNVETFGTEQGVTRGQIAAAVRKVFDLRPAA
HHHHHHHHCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHH
IIDELDLKRPIYLKTAAYGHFGRTDVEFPWEKTDKVEELKAAIAAE
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA