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The map label for this gene is pafA [H]

Identifier: 213692977

GI number: 213692977

Start: 2347190

End: 2348647

Strand: Reverse

Name: pafA [H]

Synonym: Blon_2124

Alternate gene names: 213692977

Gene position: 2348647-2347190 (Counterclockwise)

Preceding gene: 213692978

Following gene: 213692973

Centisome position: 82.91

GC content: 64.88

Gene sequence:

>1458_bases
ATGCCCCAACTGCGTGACAGCGGCACCCGCTCCCTGCACGCCACGGAACCCGTACCATCCGCCGAGATGGACGGGTTCTG
CCGTATTTTCGGCGTCGAAACCGAATACGGCGTGGCCGTCACCGGTGCCGAACGCCCCGTGGACGCCGGCCAGGTGGCGA
TGACCATGTTCCAGCCCATCGTCTCCCGTTCCCGCTCCACCAACACGTATCTGGCCAATGGCTCACGTCTGTATCTGGAC
GTCGGATCCCATCCCGAATACGCCACCGCCGAGGCCCGTGACCCGCGCGAGGCCTTGGGGCAGGATCTTGCCGGCGAACA
TGTGATGCGCAATCTGGCCCTGAAGGCCCAGCGCAAGCTGCGCGAATCCTACAGCGAACACGCCACGATCCACGTGTTCA
AAAACAACGTCGACTCCGCCGGCCACGCCTTCGGCTGCCACGAAAACTACCTGGTCCGCCGCTTTGTGCCGTTGGAGACC
ATCGAACACCAGCTGCTGCCGTTCCTTATCACCCGCCAGCTGTACACCGGTGCCGGCCGCATGACACCGGATGGCTTCCA
GATCACCCAGCGTGCGGACTTCCTGGACGAGGCCGTCTCCTCGGCCACCACCCGCTCCCGCCCGATGGTCAACACGCGCG
ACGAACCGCACGCCGACCCCGACTCCTTCCGCCGGCTGCATGTGATCATCGGCGACTCGAACCGTTCGCAATGGTCCACA
TGGATGAAGCTCGCCGTCACGCACCTTGTGCTGTGCGCGATCGAAGATGCCTTCCGCCTCGGCACCCCGTCCGGTTTTGA
ACACTGCGCCTTCGCCGACCCGGCTGCGGCCAATCGCACCGTCAGCCGATTCCTCGACGACCCGCACGCCGAACTGACGC
TCGAATCAGGCGAATCCGTGTCCGCACTGGGTCTGCAGCGCCGGTATTATGCGGCGGTCAAGGCCTTCATCGAAACCCAT
GGCGATGCGCTGGCAAGTTCGCTGCCGGCCACCACGATCGTCACGATTATGGGGGAGTGGAGCCGCGTTCTGGACGCGCT
GGAGCGTGGTGCATACGATGCGCTCGCCGACCGCGTGGACTGGGCCGCCAAGAAGCGTCTGTTCGATGCGCTCAAACGCC
GTCGCCCGGACGTCACCTTCGCCCAGATGGAACAGCTTGAGCTTGACTACCACGACATCGCCAATGGCCGCCTGTACGGT
TCGCTGACTTCCCGCAACCAAATGCGTGAGCTGCTGACAGGAGACGACGTGGAATACGCCGTGCACAACCCGCCGACCGA
CACCCGTGCCGCCCTGCGTGGCCGGTTCGTGGACGCGGCCCTGAACGTCGGAGCCCAGTTCTCCGCCGACTGGACCCACC
TGACCCTGACCGCCCCCGAACGCCGGGAAGCCATACTCCTCGATCCCTTCGAGGCCGAGCCCACCCCGGAATTCGAGCAG
CTTATGGAAGCGCTGTAG

Upstream 100 bases:

>100_bases
AGGCCGACGTGTTGGACGACATCCTCGACGACATCGAATCCACCTTGGAAACCAACGCCGAGGAATACGTCAACAGCTTC
GTGCAAAAAGGCGGCGAGTG

Downstream 100 bases:

>100_bases
ATTCCACAGGAGTTCTTCACCAGCAAACCATTCAAGCTGGAGTCCGACAACTCGCTTTCCGTTGATGCGGCCGTGGAACA
TCTGCTGAGGCATGCAGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPIVSRSRSTNTYLANGSRLYLD
VGSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKLRESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLET
IEHQLLPFLITRQLYTGAGRMTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWST
WMKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESVSALGLQRRYYAAVKAFIETH
GDALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVDWAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYG
SLTSRNQMRELLTGDDVEYAVHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQ
LMEAL

Sequences:

>Translated_485_residues
MPQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPIVSRSRSTNTYLANGSRLYLD
VGSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKLRESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLET
IEHQLLPFLITRQLYTGAGRMTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWST
WMKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESVSALGLQRRYYAAVKAFIETH
GDALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVDWAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYG
SLTSRNQMRELLTGDDVEYAVHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQ
LMEAL
>Mature_484_residues
PQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPIVSRSRSTNTYLANGSRLYLDV
GSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKLRESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLETI
EHQLLPFLITRQLYTGAGRMTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWSTW
MKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESVSALGLQRRYYAAVKAFIETHG
DALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVDWAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYGS
LTSRNQMRELLTGDDVEYAVHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQL
MEAL

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022279
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 54077; Mature: 53946

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPI
CCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHH
VSRSRSTNTYLANGSRLYLDVGSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKL
HHCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
RESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLETIEHQLLPFLITRQLYTGAGR
HHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
MTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWST
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEEEEECCCCHHHHH
WMKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
SALGLQRRYYAAVKAFIETHGDALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
WAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYGSLTSRNQMRELLTGDDVEYA
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEE
VHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQ
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHH
LMEAL
HHHHC
>Mature Secondary Structure 
PQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPI
CCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHH
VSRSRSTNTYLANGSRLYLDVGSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKL
HHCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
RESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLETIEHQLLPFLITRQLYTGAGR
HHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
MTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWST
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEEEEECCCCHHHHH
WMKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
SALGLQRRYYAAVKAFIETHGDALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
WAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYGSLTSRNQMRELLTGDDVEYA
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEE
VHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQ
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHH
LMEAL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA