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The map label for this gene is prfB [H]

Identifier: 213692886

GI number: 213692886

Start: 2215169

End: 2216293

Strand: Reverse

Name: prfB [H]

Synonym: Blon_2027

Alternate gene names: 213692886

Gene position: 2216293-2215169 (Counterclockwise)

Preceding gene: 213692888

Following gene: 213692885

Centisome position: 78.24

GC content: 63.82

Gene sequence:

>1125_bases
ATGGCAGAATTTGACTTTACCCAGGCGATCGGCGAAGCCCGCGCCAAATACGAATCCATCGCCAAGGCGCTGGACATCAA
CCGGCTCAAGGCCGACATCAAGGATCTCGAAACGCAGGCCGCGGCGCCGGGCCTGTGGGATGATCCGGAAAACGCCCAGA
AGATCACCAGCCGGCTGTCCGCCGCGGAATCGCAGCTGAAGCGTCTGAACTCCGCCTCCCAGCGCATCGACGATGTGGAA
ACGCTCGTTGAACTCGGCCACGAGGAGGGCGACCAGGACTCTCTGGACGAGGCGCAAAGCGAAATCGGCGAGATTCAGAA
GGATCTCGACCAGATGGAGATTCAGACCCTGCTCGATGGCGAATACGACGAACGTTCCGCCGTGGTGACCATCCGCTCCG
GTGCCGGCGGCGTCGACGCGGCCGATTTCGCACAGATGCTGTTGCGCATGTACCTGCGCTACTGCGAGCGTAACGGCTTC
AAGGCCAAGGTCATGGACACCTCCTACGCCGAAGAGGCGGGCATCAAGTCCGCCACCTTCCAGGTGGATGCCCCCTATGC
TTACGGCCGACTGTCCGTGGAGGGCGGCACCCACCGTCTGGTGCGCATCTCCCCGTTCGACAACCAGGGCCGCCGCCAGA
CCAGCTTCGCCGCCGTGGAAGTGGTGCCGCTGGTCGAGGCCACCGATCACATCGACATTCCGGATACGGACATCCGCGTG
GACACCTACTGCTCCTCCGGCCCCGGCGGCCAGGGCGTGAACACCACGTACTCGGCCGTGCGCATCACCCACCTTCCCAC
CGGCATCGTGGTGACCATGCAGGATGAGCGTTCGCAGATTCAGAACCGTGCCTCCGCCATGGCCGTGCTCCAGTCCCGAC
TGCTCGTGCTGCGCCACGAGGAGGAGGCCAAGAAGAAGAAAGAGCTCGCCGGCGACATCAAGGCCAGCTGGGGCGATCAG
ATGCGCTCCTACGTGCTGCACCCCTACCAGATGGTCAAGGATCTGCGCACCGGCTACGAAACCAGCCAGACCCAGGCCGT
GTTCGACGGCGACATCAACGCGTTCATCGAGGCCGGCATCCGCTGGCGCCATGAACAGCGCCGCGCCGCCGAAACCGAGG
AGTAA

Upstream 100 bases:

>100_bases
CGCGGCAGTGCCCGCCAACGCCAGAGGAATGTGCGAAGGCTGCCAACAAGGACGTGACTTCCCAAGGAATCTCAAAGAAC
CAACAGTAAGGTAAACCACG

Downstream 100 bases:

>100_bases
GTAGCATTATGGCACTGATCTCACTGGACAATGTGTCCAAGATCTATCCCAAAGGCACCCGACCGGCGCTTGACGACATC
AACCTCGATATCGAACGCGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MAEFDFTQAIGEARAKYESIAKALDINRLKADIKDLETQAAAPGLWDDPENAQKITSRLSAAESQLKRLNSASQRIDDVE
TLVELGHEEGDQDSLDEAQSEIGEIQKDLDQMEIQTLLDGEYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGF
KAKVMDTSYAEEAGIKSATFQVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHIDIPDTDIRV
DTYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHEEEAKKKKELAGDIKASWGDQ
MRSYVLHPYQMVKDLRTGYETSQTQAVFDGDINAFIEAGIRWRHEQRRAAETEE

Sequences:

>Translated_374_residues
MAEFDFTQAIGEARAKYESIAKALDINRLKADIKDLETQAAAPGLWDDPENAQKITSRLSAAESQLKRLNSASQRIDDVE
TLVELGHEEGDQDSLDEAQSEIGEIQKDLDQMEIQTLLDGEYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGF
KAKVMDTSYAEEAGIKSATFQVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHIDIPDTDIRV
DTYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHEEEAKKKKELAGDIKASWGDQ
MRSYVLHPYQMVKDLRTGYETSQTQAVFDGDINAFIEAGIRWRHEQRRAAETEE
>Mature_373_residues
AEFDFTQAIGEARAKYESIAKALDINRLKADIKDLETQAAAPGLWDDPENAQKITSRLSAAESQLKRLNSASQRIDDVET
LVELGHEEGDQDSLDEAQSEIGEIQKDLDQMEIQTLLDGEYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGFK
AKVMDTSYAEEAGIKSATFQVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHIDIPDTDIRVD
TYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHEEEAKKKKELAGDIKASWGDQM
RSYVLHPYQMVKDLRTGYETSQTQAVFDGDINAFIEAGIRWRHEQRRAAETEE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=32.1917808219178, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=29.7385620915033, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=33.0097087378641, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=42.6470588235294, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1787462, Length=256, Percent_Identity=40.234375, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=243, Percent_Identity=29.6296296296296, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=355, Percent_Identity=30.9859154929577, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921226, Length=277, Percent_Identity=35.0180505415162, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41645; Mature: 41514

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFDFTQAIGEARAKYESIAKALDINRLKADIKDLETQAAAPGLWDDPENAQKITSRLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AAESQLKRLNSASQRIDDVETLVELGHEEGDQDSLDEAQSEIGEIQKDLDQMEIQTLLDG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGFKAKVMDTSYAEEAGIKSATF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCEEE
QVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHIDIPDTDIRV
EEECCCEECEEEECCCCEEEEEECCCCCCCCCHHHHHHEEEEEHHHCCCCCCCCCCCEEE
DTYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHE
EECCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCH
EEAKKKKELAGDIKASWGDQMRSYVLHPYQMVKDLRTGYETSQTQAVFDGDINAFIEAGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHH
RWRHEQRRAAETEE
HHHHHHHHCCCCCC
>Mature Secondary Structure 
AEFDFTQAIGEARAKYESIAKALDINRLKADIKDLETQAAAPGLWDDPENAQKITSRLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AAESQLKRLNSASQRIDDVETLVELGHEEGDQDSLDEAQSEIGEIQKDLDQMEIQTLLDG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGFKAKVMDTSYAEEAGIKSATF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCEEE
QVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHIDIPDTDIRV
EEECCCEECEEEECCCCEEEEEECCCCCCCCCHHHHHHEEEEEHHHCCCCCCCCCCCEEE
DTYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHE
EECCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCH
EEAKKKKELAGDIKASWGDQMRSYVLHPYQMVKDLRTGYETSQTQAVFDGDINAFIEAGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHH
RWRHEQRRAAETEE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA