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The map label for this gene is purD [H]

Identifier: 213692820

GI number: 213692820

Start: 2141379

End: 2142647

Strand: Direct

Name: purD [H]

Synonym: Blon_1960

Alternate gene names: 213692820

Gene position: 2141379-2142647 (Clockwise)

Preceding gene: 213692819

Following gene: 213692825

Centisome position: 75.59

GC content: 62.96

Gene sequence:

>1269_bases
ATGGGTCAGAAAGTATTGGTTATCGGCTCCGGTGCGCGTGAGCACACCATTGCCTACACGCTGCTCAAAGCGCCGAGCGT
CGATGAGGTCACCGTGGCGCCCGGCAATCCGGGCATGGTCAGGGACGGCATCCACATCACGCAGCTGAGCCAGTCGAACC
ACGCCGCACTTATCGAATTCGTGAAGAATAATGGCTATGACTGGGTGTTCGTAGGCCCGGAAGTCCCGCTGATCGAAGGC
ATCGTGGACGATTTCGCCGCCGCGGGCATCAAGGCGTTCGGCCCCTCCAAGGCCGCCGCCCAGATTGAAGGCTCCAAGAA
CTTCGCCAAGCAGCTCATGGAGCGTCATGACATCCCGACCGCCCACTACCAGACCTTCATCGACCTGGAGATGGCGCAGG
CGTACGTGCACGAGCATGGCGCCCCGATCGTCATCAAGGCCGACGGACTGGCCGCCGGCAAGGGCGTGACCGTGGCCATG
GACGAGCACACCGCCCAGCGCGCGCTCGAGGACATCTTCATCGACCACCGCTTCGGCGTCGCCGGCGCCAAGGTCGTCAT
CGAGGATTTCCTGGAAGGTCAGGAGTTCTCCCTGATGAGTTTCGTCAACGGCACCGATTTCTGGCCGATGCCGATCTCGC
AGGATCACAAGCGCGCCCATGACGGCGACGAGGGCCCGAACACCGGTGGCATGGGCGCCTACAGCCCGGTTCCGCAGATT
CCGCAGTCCGTGATCGACGAGGCCATCGAGACCATCGTGCGCCCCACCGTCGAAGGCATGGCCGAGGAAGGCACGCCGTT
CACCGGCATCCTGTACGCCGGCCTGATCGCCACCGCCGACGGCCCGAAGGTCATCGAATTCAACGCCCGTTTCGGCGACC
CGGAAACCGAAGTCGTGCTCCCGAAGCTCACCTCCGACCTCGGTGCCGCCATCGCCGCCATGCTCAACGGCGAAACCCCC
GAATTCACGTGGAATGAGTCCAACGCCACCCTCGGCGTGGTCATCGCCTCCAACGGCTACCCGGAGAATGTCATCAAGGG
TGCCCGTGTGCCCGAAATCGAGGTCGACGAGAATTCGCATGTCTACTATGCCGGCGTCACCTCCGACGACAAGGGTGGCC
TGATTGCCAACTCCGGCCGTGTCCTGCTCGTCGAAACCTCCGCCCCGGACATCAAGTCCGCCCAAGACAAGGTCTACGCG
ATCATCGACACGCTCGAGCTGCGCGGACTGTTCTACCGCCACGACATCGGTTTCAAAGCCCTGAAGTAA

Upstream 100 bases:

>100_bases
ATAACAACGAGCCCGCCTATGTGCTCGGCCAGATCACTGCCGACACCACCGGCACGCAAATCGTACTGAAGTAACTCGCT
TTCCGCAGGAAAGGAACAAC

Downstream 100 bases:

>100_bases
CAGAAGCGGCGCATCTGCCGCATATTTTGGCCCGGTGGCCATGTCAATCGATTGACGTGGCTACCGGGCCAGATTTTTCT
GTCCCGGTAGCCGATTTCGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MGQKVLVIGSGAREHTIAYTLLKAPSVDEVTVAPGNPGMVRDGIHITQLSQSNHAALIEFVKNNGYDWVFVGPEVPLIEG
IVDDFAAAGIKAFGPSKAAAQIEGSKNFAKQLMERHDIPTAHYQTFIDLEMAQAYVHEHGAPIVIKADGLAAGKGVTVAM
DEHTAQRALEDIFIDHRFGVAGAKVVIEDFLEGQEFSLMSFVNGTDFWPMPISQDHKRAHDGDEGPNTGGMGAYSPVPQI
PQSVIDEAIETIVRPTVEGMAEEGTPFTGILYAGLIATADGPKVIEFNARFGDPETEVVLPKLTSDLGAAIAAMLNGETP
EFTWNESNATLGVVIASNGYPENVIKGARVPEIEVDENSHVYYAGVTSDDKGGLIANSGRVLLVETSAPDIKSAQDKVYA
IIDTLELRGLFYRHDIGFKALK

Sequences:

>Translated_422_residues
MGQKVLVIGSGAREHTIAYTLLKAPSVDEVTVAPGNPGMVRDGIHITQLSQSNHAALIEFVKNNGYDWVFVGPEVPLIEG
IVDDFAAAGIKAFGPSKAAAQIEGSKNFAKQLMERHDIPTAHYQTFIDLEMAQAYVHEHGAPIVIKADGLAAGKGVTVAM
DEHTAQRALEDIFIDHRFGVAGAKVVIEDFLEGQEFSLMSFVNGTDFWPMPISQDHKRAHDGDEGPNTGGMGAYSPVPQI
PQSVIDEAIETIVRPTVEGMAEEGTPFTGILYAGLIATADGPKVIEFNARFGDPETEVVLPKLTSDLGAAIAAMLNGETP
EFTWNESNATLGVVIASNGYPENVIKGARVPEIEVDENSHVYYAGVTSDDKGGLIANSGRVLLVETSAPDIKSAQDKVYA
IIDTLELRGLFYRHDIGFKALK
>Mature_421_residues
GQKVLVIGSGAREHTIAYTLLKAPSVDEVTVAPGNPGMVRDGIHITQLSQSNHAALIEFVKNNGYDWVFVGPEVPLIEGI
VDDFAAAGIKAFGPSKAAAQIEGSKNFAKQLMERHDIPTAHYQTFIDLEMAQAYVHEHGAPIVIKADGLAAGKGVTVAMD
EHTAQRALEDIFIDHRFGVAGAKVVIEDFLEGQEFSLMSFVNGTDFWPMPISQDHKRAHDGDEGPNTGGMGAYSPVPQIP
QSVIDEAIETIVRPTVEGMAEEGTPFTGILYAGLIATADGPKVIEFNARFGDPETEVVLPKLTSDLGAAIAAMLNGETPE
FTWNESNATLGVVIASNGYPENVIKGARVPEIEVDENSHVYYAGVTSDDKGGLIANSGRVLLVETSAPDIKSAQDKVYAI
IDTLELRGLFYRHDIGFKALK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=44.6261682242991, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=44.6261682242991, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=44.6261682242991, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=44.6261682242991, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=46.6042154566745, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=36.3420427553444, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=38.8636363636364, Blast_Score=302, Evalue=7e-83,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=40.0921658986175, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=40.0921658986175, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45238; Mature: 45106

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQKVLVIGSGAREHTIAYTLLKAPSVDEVTVAPGNPGMVRDGIHITQLSQSNHAALIEF
CCCEEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCCEEECCEEEEEECCCCCCEEEHH
VKNNGYDWVFVGPEVPLIEGIVDDFAAAGIKAFGPSKAAAQIEGSKNFAKQLMERHDIPT
HHCCCCEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEECCCHHHHHHHHHHCCCCH
AHYQTFIDLEMAQAYVHEHGAPIVIKADGLAAGKGVTVAMDEHTAQRALEDIFIDHRFGV
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCC
AGAKVVIEDFLEGQEFSLMSFVNGTDFWPMPISQDHKRAHDGDEGPNTGGMGAYSPVPQI
CHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
PQSVIDEAIETIVRPTVEGMAEEGTPFTGILYAGLIATADGPKVIEFNARFGDPETEVVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEECCCCCCCCEEEE
PKLTSDLGAAIAAMLNGETPEFTWNESNATLGVVIASNGYPENVIKGARVPEIEVDENSH
HHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCHHHHCCCCCCEEEECCCCE
VYYAGVTSDDKGGLIANSGRVLLVETSAPDIKSAQDKVYAIIDTLELRGLFYRHDIGFKA
EEEEEECCCCCCCEEECCCCEEEEECCCCCCCCHHCCEEEEEEHHHHCEEEEEECCCCEE
LK
CC
>Mature Secondary Structure 
GQKVLVIGSGAREHTIAYTLLKAPSVDEVTVAPGNPGMVRDGIHITQLSQSNHAALIEF
CCEEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCCEEECCEEEEEECCCCCCEEEHH
VKNNGYDWVFVGPEVPLIEGIVDDFAAAGIKAFGPSKAAAQIEGSKNFAKQLMERHDIPT
HHCCCCEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEECCCHHHHHHHHHHCCCCH
AHYQTFIDLEMAQAYVHEHGAPIVIKADGLAAGKGVTVAMDEHTAQRALEDIFIDHRFGV
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCC
AGAKVVIEDFLEGQEFSLMSFVNGTDFWPMPISQDHKRAHDGDEGPNTGGMGAYSPVPQI
CHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
PQSVIDEAIETIVRPTVEGMAEEGTPFTGILYAGLIATADGPKVIEFNARFGDPETEVVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCEEEEEECCCCCCCCEEEE
PKLTSDLGAAIAAMLNGETPEFTWNESNATLGVVIASNGYPENVIKGARVPEIEVDENSH
HHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCHHHHCCCCCCEEEECCCCE
VYYAGVTSDDKGGLIANSGRVLLVETSAPDIKSAQDKVYAIIDTLELRGLFYRHDIGFKA
EEEEEECCCCCCCEEECCCCEEEEECCCCCCCCHHCCEEEEEEHHHHCEEEEEECCCCEE
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]