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The map label for this gene is purF [H]

Identifier: 213692818

GI number: 213692818

Start: 2138682

End: 2140193

Strand: Direct

Name: purF [H]

Synonym: Blon_1958

Alternate gene names: 213692818

Gene position: 2138682-2140193 (Clockwise)

Preceding gene: 213692817

Following gene: 213692819

Centisome position: 75.5

GC content: 62.17

Gene sequence:

>1512_bases
TTGTCGGCTGAACTCGAAGACATTCATGAGGAATGTGGCATCTTCGGCGTATGGGGCCACCCCGACGCCTCACGACTCAC
CTATTTTGGCCTGCACGCGCTGCAGCATCGCGGTCAGGAAGGTGCCGGCATCGTCTCCAACGATAACGGTCATCTCATCG
GTCATCGCGGACTCGGCCTGCTCACCCAGGTGTTCGGCGATGAGCGCGAAATCGAACGACTGAAGGGCAATTGCGCCATC
GGCCACGTGCGTTACGCAACGGCCGGCTCGGGCACCACGGACAACATCCAGCCGTTCATCTTCCGCTTCCATGACGGCGA
CGTGGCTCTGTGCCATAACGGCAACCTGACGAACTGCCCGTCACTGCGCCGCAAGCTTGAAGACGAAGGCGCCATCTTCC
ATTCGAACTCCGACACCGAAGTGCTGATGCACTTGATTCGCCGTTCGATGCAGCGCACCTTTATGGACAAGCTCAAGGAA
GCGCTCAACACGGTGCATGGCGGTTTCGCCTACCTGCTGATGACCGAAGACGCCATGATCGGCGCACTCGACCCGAACGG
CTTCCGCCCGCTCTCCCTCGGCAAGATGAAGAACGGCGCCTACGTGCTCGCCTCCGAAACCTGCGCCCTCGACGTGGTCG
GCGCCGAACTGGTGCGCAACATCCGCCCCGGCGAAATCGTGGTGGTCAACGACCACGGCTACAAGATCGTGCAGTACACC
AACAACACGCAGCTGGCCATCTGCTCGATGGAATACATCTACTTCGCCCGTCCGGATTCCGACATCTACGGCGTCAACGT
GCACTCCGCCCGCAAGCGCATGGGCGCGCGTCTGGCGCAGGAGTCCCCGGTCGAGGCCGATATGGTCATCGGCGTGCCGA
ACTCCTCCCTGTCCGCGGCGTCCGGCTACGCGGAGGCCGCCGGGCTGCCCAACGAAATGGGTCTGATCAAGAACCAGTAC
GTGGCCCGCACCTTCATCCAGCCCACACAGGAACTGCGCGAACAGGGCGTGCGCATGAAGCTGTCCGCCGTGCGTGGCGT
GGTCAAAGGCAAGCGCGTGGTCGTCATCGACGATTCGATCGTGCGTGGCACCACCTCCAAGCGCATCGTGCAGCTGCTGC
GCGAAGCCGGCGCCGCCGAAGTGCACATGCGCATCAGCTCGCCGCCACTCAAATACCCGTGCTTCTACGGCATCGACATC
TCCACCACCAAGGAACTCATCGCCGCCGAGAAAAGCGTCGAGGAGATCCGCGAATACATCGGTGCCGACTCGCTGGCCTA
CCTGTCGCTGGACGGTCTGGTCGAATCGATCGGCCTGAACGCCGACGCCCCTTACGGAGGCCTGTGCGTCGCCTACTTCA
ACGGCGACTACCCCACCGCGCTCGACGACTATGAGACCGACTTCCTGAAATCCCTCACGCCCGAAGACCGCGTGCGCCTG
CCCGAATTCGCGCTGTACAAGAGCAAGTACATCGGCAACGAATACAACACCGTGCAGCCGGAAGCCGAATAG

Upstream 100 bases:

>100_bases
CGAACCCCAACAAGTTCCACCACCCCACAACACGCGATGTAGCCGCCGATTGGTAGGGTTGGCAGATAGTTAATCCCCTG
TTTGAAAAAGGAGCTTTGGC

Downstream 100 bases:

>100_bases
CTCACCACCAATCCACTCAATAACAATTCTTTGAACCAGAGGCGCTTGCCTCCCCTCAGTCGGCTTTGCCGACAGCCCCC
CCCTCCAGAGGGGAGCCAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSAELEDIHEECGIFGVWGHPDASRLTYFGLHALQHRGQEGAGIVSNDNGHLIGHRGLGLLTQVFGDEREIERLKGNCAI
GHVRYATAGSGTTDNIQPFIFRFHDGDVALCHNGNLTNCPSLRRKLEDEGAIFHSNSDTEVLMHLIRRSMQRTFMDKLKE
ALNTVHGGFAYLLMTEDAMIGALDPNGFRPLSLGKMKNGAYVLASETCALDVVGAELVRNIRPGEIVVVNDHGYKIVQYT
NNTQLAICSMEYIYFARPDSDIYGVNVHSARKRMGARLAQESPVEADMVIGVPNSSLSAASGYAEAAGLPNEMGLIKNQY
VARTFIQPTQELREQGVRMKLSAVRGVVKGKRVVVIDDSIVRGTTSKRIVQLLREAGAAEVHMRISSPPLKYPCFYGIDI
STTKELIAAEKSVEEIREYIGADSLAYLSLDGLVESIGLNADAPYGGLCVAYFNGDYPTALDDYETDFLKSLTPEDRVRL
PEFALYKSKYIGNEYNTVQPEAE

Sequences:

>Translated_503_residues
MSAELEDIHEECGIFGVWGHPDASRLTYFGLHALQHRGQEGAGIVSNDNGHLIGHRGLGLLTQVFGDEREIERLKGNCAI
GHVRYATAGSGTTDNIQPFIFRFHDGDVALCHNGNLTNCPSLRRKLEDEGAIFHSNSDTEVLMHLIRRSMQRTFMDKLKE
ALNTVHGGFAYLLMTEDAMIGALDPNGFRPLSLGKMKNGAYVLASETCALDVVGAELVRNIRPGEIVVVNDHGYKIVQYT
NNTQLAICSMEYIYFARPDSDIYGVNVHSARKRMGARLAQESPVEADMVIGVPNSSLSAASGYAEAAGLPNEMGLIKNQY
VARTFIQPTQELREQGVRMKLSAVRGVVKGKRVVVIDDSIVRGTTSKRIVQLLREAGAAEVHMRISSPPLKYPCFYGIDI
STTKELIAAEKSVEEIREYIGADSLAYLSLDGLVESIGLNADAPYGGLCVAYFNGDYPTALDDYETDFLKSLTPEDRVRL
PEFALYKSKYIGNEYNTVQPEAE
>Mature_502_residues
SAELEDIHEECGIFGVWGHPDASRLTYFGLHALQHRGQEGAGIVSNDNGHLIGHRGLGLLTQVFGDEREIERLKGNCAIG
HVRYATAGSGTTDNIQPFIFRFHDGDVALCHNGNLTNCPSLRRKLEDEGAIFHSNSDTEVLMHLIRRSMQRTFMDKLKEA
LNTVHGGFAYLLMTEDAMIGALDPNGFRPLSLGKMKNGAYVLASETCALDVVGAELVRNIRPGEIVVVNDHGYKIVQYTN
NTQLAICSMEYIYFARPDSDIYGVNVHSARKRMGARLAQESPVEADMVIGVPNSSLSAASGYAEAAGLPNEMGLIKNQYV
ARTFIQPTQELREQGVRMKLSAVRGVVKGKRVVVIDDSIVRGTTSKRIVQLLREAGAAEVHMRISSPPLKYPCFYGIDIS
TTKELIAAEKSVEEIREYIGADSLAYLSLDGLVESIGLNADAPYGGLCVAYFNGDYPTALDDYETDFLKSLTPEDRVRLP
EFALYKSKYIGNEYNTVQPEAE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=468, Percent_Identity=39.7435897435897, Blast_Score=341, Evalue=1e-93,
Organism=Escherichia coli, GI1788651, Length=490, Percent_Identity=38.7755102040816, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1790167, Length=202, Percent_Identity=29.2079207920792, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=41.4583333333333, Blast_Score=344, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=36.0248447204969, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=40.1639344262295, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=40.6852248394004, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=39, Blast_Score=341, Evalue=9e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55348; Mature: 55217

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAELEDIHEECGIFGVWGHPDASRLTYFGLHALQHRGQEGAGIVSNDNGHLIGHRGLGL
CCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCHHH
LTQVFGDEREIERLKGNCAIGHVRYATAGSGTTDNIQPFIFRFHDGDVALCHNGNLTNCP
HHHHHCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCH
SLRRKLEDEGAIFHSNSDTEVLMHLIRRSMQRTFMDKLKEALNTVHGGFAYLLMTEDAMI
HHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
GALDPNGFRPLSLGKMKNGAYVLASETCALDVVGAELVRNIRPGEIVVVNDHGYKIVQYT
EEECCCCCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHCCCCCEEEEECCCCEEEEEEE
NNTQLAICSMEYIYFARPDSDIYGVNVHSARKRMGARLAQESPVEADMVIGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
SGYAEAAGLPNEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVKGKRVVVIDDSI
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCEEEEECCCH
VRGTTSKRIVQLLREAGAAEVHMRISSPPLKYPCFYGIDISTTKELIAAEKSVEEIREYI
HCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
GADSLAYLSLDGLVESIGLNADAPYGGLCVAYFNGDYPTALDDYETDFLKSLTPEDRVRL
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCHHHCCC
PEFALYKSKYIGNEYNTVQPEAE
CHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SAELEDIHEECGIFGVWGHPDASRLTYFGLHALQHRGQEGAGIVSNDNGHLIGHRGLGL
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCHHH
LTQVFGDEREIERLKGNCAIGHVRYATAGSGTTDNIQPFIFRFHDGDVALCHNGNLTNCP
HHHHHCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCH
SLRRKLEDEGAIFHSNSDTEVLMHLIRRSMQRTFMDKLKEALNTVHGGFAYLLMTEDAMI
HHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
GALDPNGFRPLSLGKMKNGAYVLASETCALDVVGAELVRNIRPGEIVVVNDHGYKIVQYT
EEECCCCCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHCCCCCEEEEECCCCEEEEEEE
NNTQLAICSMEYIYFARPDSDIYGVNVHSARKRMGARLAQESPVEADMVIGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
SGYAEAAGLPNEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVKGKRVVVIDDSI
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCEEEEECCCH
VRGTTSKRIVQLLREAGAAEVHMRISSPPLKYPCFYGIDISTTKELIAAEKSVEEIREYI
HCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
GADSLAYLSLDGLVESIGLNADAPYGGLCVAYFNGDYPTALDDYETDFLKSLTPEDRVRL
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCHHHCCC
PEFALYKSKYIGNEYNTVQPEAE
CHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA