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The map label for this gene is thiG [H]

Identifier: 213692738

GI number: 213692738

Start: 2041022

End: 2041891

Strand: Reverse

Name: thiG [H]

Synonym: Blon_1872

Alternate gene names: 213692738

Gene position: 2041891-2041022 (Counterclockwise)

Preceding gene: 213692739

Following gene: 213692737

Centisome position: 72.08

GC content: 62.53

Gene sequence:

>870_bases
ATGAGCAATATTAATCCGGTTACTGAAGCATCTGTTGAGGCGACTCCGCTGCCGCCGCAGCCCGCCGTCACCAATCCGGT
CGGCACCGCCGCGCCGATTCTGCCAGTGGAAGACAAGCCGCTGACCCTGGGCGGTCACGAGTTCCAGTCGCGATTCATTC
TGGGCTCCGGCCGCTACGACCTGAACCTCATCAAGGCCACGGTCGAACATGCAGGCACGCAAATCGTCACCATGGCACTG
CGCCGCGCGCAGACCACCGAGAACAGCGTGCTCGACTACATTCCCGAAGGCATCACGTTGCTGCCCAACACCTCCGGTGC
GCGCAACGCCGAGGAAGCCGTGCGTATCGCCCGACTGGCACGCGAAGTGTGCCACACCGACTTCGTCAAGGTCGAAATCG
AACACGAGACCAAGTACCTCCTGCCGGACAACGCCGAAACCATCCGAGCCACCGAAATGCTGGCCAAGGAAGGCTTCGTG
GTCATGCCCTACATGTTCCCGGACCCGATTGCCGCACGCCAGCTGGAGGAGGCCGGTGCCGCCGCCGTTATGCCGCTCGG
TTCGCTGATCGGCTCCAACATGGGCCTGCGCATGCGTGACTTCATCGAGATCATCATCGCCAATGCGCATGTGCCGGTCA
TCATCGATGCCGGCATCGGACGCCCGAGTCAGGCATGCGACGCCATGGAGATGGGTGCGGATGCGGTGATGGCGTACACG
GCCGTTGCCTCTGCTGGCAATATCCCGCTGATGGCTGAGGCGTTCAAGCACGCCATCGATGCTGGTCGTGCGGCGTATCT
GTCCGGTCTGGGCAAGGTGACCGAGGGACAGGCCGTGCCGTCCAGTCCGACTACGGGATATTTGCACTGA

Upstream 100 bases:

>100_bases
GTGAGATTATCCCGCGCGATAAGCGTGCCGACGCCATGCTCGACGACCAGTGCGTCATGGAAATTGTGCAGTTTGTACAG
GGTGGATGAAAGGAAAAGCT

Downstream 100 bases:

>100_bases
CACTTTTATTAGCTCCCCTCTCTGAGGGGAGCTGGCCGCGTAGCGGACTGAGGGGAGCTGGAAACATGGAGAAAACATGG
CATTGACTGATGAACAGGTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSNINPVTEASVEATPLPPQPAVTNPVGTAAPILPVEDKPLTLGGHEFQSRFILGSGRYDLNLIKATVEHAGTQIVTMAL
RRAQTTENSVLDYIPEGITLLPNTSGARNAEEAVRIARLAREVCHTDFVKVEIEHETKYLLPDNAETIRATEMLAKEGFV
VMPYMFPDPIAARQLEEAGAAAVMPLGSLIGSNMGLRMRDFIEIIIANAHVPVIIDAGIGRPSQACDAMEMGADAVMAYT
AVASAGNIPLMAEAFKHAIDAGRAAYLSGLGKVTEGQAVPSSPTTGYLH

Sequences:

>Translated_289_residues
MSNINPVTEASVEATPLPPQPAVTNPVGTAAPILPVEDKPLTLGGHEFQSRFILGSGRYDLNLIKATVEHAGTQIVTMAL
RRAQTTENSVLDYIPEGITLLPNTSGARNAEEAVRIARLAREVCHTDFVKVEIEHETKYLLPDNAETIRATEMLAKEGFV
VMPYMFPDPIAARQLEEAGAAAVMPLGSLIGSNMGLRMRDFIEIIIANAHVPVIIDAGIGRPSQACDAMEMGADAVMAYT
AVASAGNIPLMAEAFKHAIDAGRAAYLSGLGKVTEGQAVPSSPTTGYLH
>Mature_288_residues
SNINPVTEASVEATPLPPQPAVTNPVGTAAPILPVEDKPLTLGGHEFQSRFILGSGRYDLNLIKATVEHAGTQIVTMALR
RAQTTENSVLDYIPEGITLLPNTSGARNAEEAVRIARLAREVCHTDFVKVEIEHETKYLLPDNAETIRATEMLAKEGFVV
MPYMFPDPIAARQLEEAGAAAVMPLGSLIGSNMGLRMRDFIEIIIANAHVPVIIDAGIGRPSQACDAMEMGADAVMAYTA
VASAGNIPLMAEAFKHAIDAGRAAYLSGLGKVTEGQAVPSSPTTGYLH

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=46.4285714285714, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 30698; Mature: 30566

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNINPVTEASVEATPLPPQPAVTNPVGTAAPILPVEDKPLTLGGHEFQSRFILGSGRYD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCHHHHCEEEEECCCCC
LNLIKATVEHAGTQIVTMALRRAQTTENSVLDYIPEGITLLPNTSGARNAEEAVRIARLA
HHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
REVCHTDFVKVEIEHETKYLLPDNAETIRATEMLAKEGFVVMPYMFPDPIAARQLEEAGA
HHHHCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCC
AAVMPLGSLIGSNMGLRMRDFIEIIIANAHVPVIIDAGIGRPSQACDAMEMGADAVMAYT
CHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHH
AVASAGNIPLMAEAFKHAIDAGRAAYLSGLGKVTEGQAVPSSPTTGYLH
HHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNINPVTEASVEATPLPPQPAVTNPVGTAAPILPVEDKPLTLGGHEFQSRFILGSGRYD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCHHHHCEEEEECCCCC
LNLIKATVEHAGTQIVTMALRRAQTTENSVLDYIPEGITLLPNTSGARNAEEAVRIARLA
HHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
REVCHTDFVKVEIEHETKYLLPDNAETIRATEMLAKEGFVVMPYMFPDPIAARQLEEAGA
HHHHCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCC
AAVMPLGSLIGSNMGLRMRDFIEIIIANAHVPVIIDAGIGRPSQACDAMEMGADAVMAYT
CHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHH
AVASAGNIPLMAEAFKHAIDAGRAAYLSGLGKVTEGQAVPSSPTTGYLH
HHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]