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The map label for this gene is tyrS

Identifier: 213692733

GI number: 213692733

Start: 2036820

End: 2038142

Strand: Reverse

Name: tyrS

Synonym: Blon_1867

Alternate gene names: 213692733

Gene position: 2038142-2036820 (Counterclockwise)

Preceding gene: 213692735

Following gene: 213692732

Centisome position: 71.95

GC content: 61.98

Gene sequence:

>1323_bases
ATGGCTCACGTCACCGACTTCAAGGAAGCGGGTTTCAACACCCTGCTTGAGGAACTGGAATGGCGCGGGTTGATTTCCCA
GTCCACGGACCGGGATCGACTCGCAGAAGCACTGAACGGCGAGCCCATCACCTATTATTGCGGCTTCGACCCCACTGCTG
CATCGTTGCATATCGGCAACCTCGTGCAGCTCATCAACATGCGTCACCTGCAGCTTGCGGGCCATCATCCAATCGCCCTG
GTCGGCGGCGCCACCGGACTCATCGGCGACCCGCGCCAGTCCGGCGAACGCACGCTCAACCCGAAGGACGTGGTGGCCGG
CTGGGCCGATCGTCTGAAGAACCAGATCGGCGGCATTCTGGACACCGAAGGCGCGAACGCCGTACGTTTCGTGTCCAATT
ACGATTGGACCGCCTCGATGACCGTCATCGACTTCCTGCGTGACGTCGGCAAGAACTTCCGTCTGGGCACCATGCTCGCC
AAGGACACGGTCGCCCGTCGCCTGAACTCTGAGGAAGGCATCTCCTTCACCGAGTTCAGCTACCAGGTGCTGCAGGGCAA
CGACTTCCTCCACCTGTTTGACGAATACCACTGCACGCTGGAACTCGGCGGCTCCGACCAGTGGGGCAACCTCACCTCCG
GCCTGGACCTGATCCACAAGGTGCGCGGTGTGGACGTCAACGTGTTCACCAGCCCGATCATCACTGATGCTTCCGGCAAG
AAGTTCGGTAAGTCCGAGGGCAACGCCGTCTGGCTCGACGCCACGATGCTCAGCCCGTACAAGTTCTACCAGTTCTGGAT
CAACCGCCCGGACGTGGAGATGGAAAGCCTGCTGAAGGCGTTCACCTTCCTGCCCAAGGCCGAAATCGAACGACTGGTCG
AGGAATCCAAGACCAACCCCGGCAAGCGCGAGGCCCAGAAGACCCTCGCCTGGGAGGTCACCAGCTTCGTGCATGGCGAG
GCCGCTACCCAGGCTGCCATCGATGCTTCCGGTGCGCTGTTCGGCCGCGGTGGCAACCTCGAAGACATCGACGAGGAAAC
GCTGGAATCCGTGCTCGACGGCTTCAAGGTGGTCGACGAGAACGGCGAGCACGTCTTCCCCGTCTCCAAGCCGGGCGATC
GCGTAATCGACGCCGCCCAGGCTGCCGGACTGTTCAAGTCCGCTTCCGAGGCACGCCGCGCCATCAAGTCCGGTGGCGTG
TATCTCAACAACAACCGTATCGAGGATGAGGAGCAGGTGCTGGCCGAAGCCGACTTCCTGGCAGGTCGCTTCGCGCTCAT
CCGTCGTGGCAAGAAGGCGCTCGGCGCCGTGGAAAACCGTTAA

Upstream 100 bases:

>100_bases
GGCTCGGCTGGTGTTCGGCACTGTCGCCATGTTCTGCGAAACTTGACTGTGCCCAATTGGGCAGTCTCATTCACAATTCG
ATAGGGAGAAGCGACAAGCT

Downstream 100 bases:

>100_bases
TCAACAACGAATTAAGGAAAACAAGCAATGGCAGAAGGTAACGAGCGTTCCGGCAAGTCCTACGGCGGCGGCCATAAGGG
GTATGGCGATCGTCGACCGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGNLVQLINMRHLQLAGHHPIAL
VGGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGILDTEGANAVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLA
KDTVARRLNSEEGISFTEFSYQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGK
KFGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNPGKREAQKTLAWEVTSFVHGE
AATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDENGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGV
YLNNNRIEDEEQVLAEADFLAGRFALIRRGKKALGAVENR

Sequences:

>Translated_440_residues
MAHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGNLVQLINMRHLQLAGHHPIAL
VGGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGILDTEGANAVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLA
KDTVARRLNSEEGISFTEFSYQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGK
KFGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNPGKREAQKTLAWEVTSFVHGE
AATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDENGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGV
YLNNNRIEDEEQVLAEADFLAGRFALIRRGKKALGAVENR
>Mature_439_residues
AHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGNLVQLINMRHLQLAGHHPIALV
GGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGILDTEGANAVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLAK
DTVARRLNSEEGISFTEFSYQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGKK
FGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNPGKREAQKTLAWEVTSFVHGEA
ATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDENGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGVY
LNNNRIEDEEQVLAEADFLAGRFALIRRGKKALGAVENR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=409, Percent_Identity=39.3643031784841, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=45.9905660377358, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145802, Length=375, Percent_Identity=36.5333333333333, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25145805, Length=268, Percent_Identity=35.4477611940299, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=453, Percent_Identity=35.9823399558499, Blast_Score=269, Evalue=7e-73,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=39.7701149425287, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_BIFLD (B3DSX9)

Other databases:

- EMBL:   CP000605
- RefSeq:   YP_001954746.1
- GeneID:   6362916
- GenomeReviews:   CP000605_GR
- KEGG:   blj:BLD_0802
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 48413; Mature: 48282

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 46-46 BINDING 181-181 BINDING 185-185 BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGN
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
LVQLINMRHLQLAGHHPIALVGGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGIL
HHHHHHHHHHEECCCCCEEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCEE
DTEGANAVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLAKDTVARRLNSEEGISFTEFS
ECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCEEHHEE
YQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGK
EEEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCEEECCCCC
KFGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNP
CCCCCCCCEEEEEEHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GKREAQKTLAWEVTSFVHGEAATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCEEECC
NGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGVYLNNNRIEDEEQVLAEADFL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHH
AGRFALIRRGKKALGAVENR
HHHHHHHHCCHHHHCCCCCC
>Mature Secondary Structure 
AHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGN
CCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
LVQLINMRHLQLAGHHPIALVGGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGIL
HHHHHHHHHHEECCCCCEEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCEE
DTEGANAVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLAKDTVARRLNSEEGISFTEFS
ECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCEEHHEE
YQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGK
EEEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCEEECCCCC
KFGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNP
CCCCCCCCEEEEEEHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GKREAQKTLAWEVTSFVHGEAATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCEEECC
NGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGVYLNNNRIEDEEQVLAEADFL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHH
AGRFALIRRGKKALGAVENR
HHHHHHHHCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA