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The map label for this gene is eno [H]

Identifier: 213692703

GI number: 213692703

Start: 1998485

End: 1999783

Strand: Reverse

Name: eno [H]

Synonym: Blon_1836

Alternate gene names: 213692703

Gene position: 1999783-1998485 (Counterclockwise)

Preceding gene: 213692704

Following gene: 213692702

Centisome position: 70.6

GC content: 63.2

Gene sequence:

>1299_bases
GTGGCAGTAATTGAAAGCGTGTACGCGCGTCAGATTCTGGATTCCCGTGGCAACCCGACCGTTGAGGTCTACCTGGAGAC
CGAAGACGGCGCTCAGGGCAAGGGTCTGGTTCCGTCCGGCGCCTCCACCGGTGAAGCTGAGGCTTGGGAGCGTCGCGATG
GCGACAAGTCCGTCTACGGCGGCAAGGGTGTTCTTAACGCGGTCAAGGCCGTGAACGAGGTCATCGCTCCGAAGGTCATC
GGCATGGACGCTGCTGACCAGCGCGCTCTGGACGACCTGATGATCGAGCTCGACGGCACCCCGAACAAGGGCAAGCTGGG
CGCCAACGCCATCCTGGGCGTCTCCCTGGCTGCTCTGTACGCTTCCGCCGAGTCCGCCGGCCTGCCGCTGTACCGCTACA
TCGGCGGCACCAACGGACACATCCTGCCGGTCCCGAACATGAACATCATGAACGGTGGCGCTCACGCTGACTTCGCCACC
GACATTCAGGAGTACATGATCTCCCCGTACGGCTTCGACACCTACTCCGAGGCTCTGCGCGCTGGCGTTGAGGTCTACCA
CACCCTGAAGAACGTCCTGAAGAAGGAAGGCCTGAACACCGGCCTCGGCGACGAGGGCGGCTTCGCTCCGAAGATGAAGT
CCAACGAGGACTCCCTCAAGTACATCATGGACGCCATCTCCGCCGCCGGCTACGAGCCCGGCAAGCAGATCGGCATCTGC
CTGGATGTCGCCTCCTCCGAGTTCTACAACAAGGAGACCGGCAAGTACCGCTTCGACGGTGAGGAGCGCGACTCCGCCTA
CATGCTCGACTACTACGAGAACCTCATCAACGAGTACCCGATCGTCTCCATCGAGGACCCGTTCAACGAGGAAGGCTGGG
AAGACTGGGCTGCCATCACCGCCCGCCTCGGCGATCGTCTGCAGTTCGTCGGCGACGACCTGCTGGTCACCAACCCGGCC
CGTCTGCAGAAGGCCATCGACCTCGGCGCCGCCAACTCCCTGCTGGTCAAGCTGAACCAGATCGGTTCCGTCACCGAGAC
CCTCGACGCCATCGAGCTGGCCACCGCCAACGGCTACACTTCCATGGTTTCCCACCGCTCCGGCGAGACCCCGGACACCA
CCATCTCCGACCTGGCTGTTGCTAAGAACACCCGCCAGATCAAGACCGGTGCCCCGGCCCGTGGCGAGCGCGTTGCCAAG
TACAACCGCCTGCTCGAGATCGAGGAGGAGCTCGGCTCCACCGCTCAGTACGCCGGCTACACCGCTTTCAAGGCCTGCAA
GAAGTACCTGGCCAAGTGA

Upstream 100 bases:

>100_bases
GCGCTAACACTAAATTTTGGGCGTGTTGTTCACAACGTCCACAAATCGCACTACTCTTAAGAATGTCATTCAAGATACAC
AACCGTTTTAAGGAGTAGTT

Downstream 100 bases:

>100_bases
GCTGAAACGCCCGTAAAGGCGTGAGTTTCCACTGAAGCTCGATACCCGTCGTGTTCGTCAAGTCGAATGCGGCGGGTATT
TTCATGTGACATGAGCAAGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAVIESVYARQILDSRGNPTVEVYLETEDGAQGKGLVPSGASTGEAEAWERRDGDKSVYGGKGVLNAVKAVNEVIAPKVI
GMDAADQRALDDLMIELDGTPNKGKLGANAILGVSLAALYASAESAGLPLYRYIGGTNGHILPVPNMNIMNGGAHADFAT
DIQEYMISPYGFDTYSEALRAGVEVYHTLKNVLKKEGLNTGLGDEGGFAPKMKSNEDSLKYIMDAISAAGYEPGKQIGIC
LDVASSEFYNKETGKYRFDGEERDSAYMLDYYENLINEYPIVSIEDPFNEEGWEDWAAITARLGDRLQFVGDDLLVTNPA
RLQKAIDLGAANSLLVKLNQIGSVTETLDAIELATANGYTSMVSHRSGETPDTTISDLAVAKNTRQIKTGAPARGERVAK
YNRLLEIEEELGSTAQYAGYTAFKACKKYLAK

Sequences:

>Translated_432_residues
MAVIESVYARQILDSRGNPTVEVYLETEDGAQGKGLVPSGASTGEAEAWERRDGDKSVYGGKGVLNAVKAVNEVIAPKVI
GMDAADQRALDDLMIELDGTPNKGKLGANAILGVSLAALYASAESAGLPLYRYIGGTNGHILPVPNMNIMNGGAHADFAT
DIQEYMISPYGFDTYSEALRAGVEVYHTLKNVLKKEGLNTGLGDEGGFAPKMKSNEDSLKYIMDAISAAGYEPGKQIGIC
LDVASSEFYNKETGKYRFDGEERDSAYMLDYYENLINEYPIVSIEDPFNEEGWEDWAAITARLGDRLQFVGDDLLVTNPA
RLQKAIDLGAANSLLVKLNQIGSVTETLDAIELATANGYTSMVSHRSGETPDTTISDLAVAKNTRQIKTGAPARGERVAK
YNRLLEIEEELGSTAQYAGYTAFKACKKYLAK
>Mature_431_residues
AVIESVYARQILDSRGNPTVEVYLETEDGAQGKGLVPSGASTGEAEAWERRDGDKSVYGGKGVLNAVKAVNEVIAPKVIG
MDAADQRALDDLMIELDGTPNKGKLGANAILGVSLAALYASAESAGLPLYRYIGGTNGHILPVPNMNIMNGGAHADFATD
IQEYMISPYGFDTYSEALRAGVEVYHTLKNVLKKEGLNTGLGDEGGFAPKMKSNEDSLKYIMDAISAAGYEPGKQIGICL
DVASSEFYNKETGKYRFDGEERDSAYMLDYYENLINEYPIVSIEDPFNEEGWEDWAAITARLGDRLQFVGDDLLVTNPAR
LQKAIDLGAANSLLVKLNQIGSVTETLDAIELATANGYTSMVSHRSGETPDTTISDLAVAKNTRQIKTGAPARGERVAKY
NRLLEIEEELGSTAQYAGYTAFKACKKYLAK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=53.1322505800464, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=53.2558139534884, Blast_Score=410, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.6279069767442, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.6279069767442, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=47.4299065420561, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI169201331, Length=209, Percent_Identity=32.0574162679426, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI169201757, Length=209, Percent_Identity=32.0574162679426, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI239744207, Length=209, Percent_Identity=32.0574162679426, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI310129182, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310110045, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310120572, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=53.3642691415313, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.7037037037037, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.7037037037037, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=52.8497409326425, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.3487297921478, Blast_Score=408, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=52.1939953810624, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=47.2350230414747, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=47.0046082949309, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=47.0046082949309, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=51.7482517482517, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=51.7482517482517, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=51.7482517482517, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=51.7482517482517, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=51.7482517482517, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=51.7482517482517, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46624; Mature: 46493

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIESVYARQILDSRGNPTVEVYLETEDGAQGKGLVPSGASTGEAEAWERRDGDKSVYG
CHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GKGVLNAVKAVNEVIAPKVIGMDAADQRALDDLMIELDGTPNKGKLGANAILGVSLAALY
CHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
ASAESAGLPLYRYIGGTNGHILPVPNMNIMNGGAHADFATDIQEYMISPYGFDTYSEALR
HHHCCCCCCEEEECCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
AGVEVYHTLKNVLKKEGLNTGLGDEGGFAPKMKSNEDSLKYIMDAISAAGYEPGKQIGIC
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSEFYNKETGKYRFDGEERDSAYMLDYYENLINEYPIVSIEDPFNEEGWEDWAAIT
EEECCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
ARLGDRLQFVGDDLLVTNPARLQKAIDLGAANSLLVKLNQIGSVTETLDAIELATANGYT
HHHCCHHHHCCCCEEEECHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
SMVSHRSGETPDTTISDLAVAKNTRQIKTGAPARGERVAKYNRLLEIEEELGSTAQYAGY
HHHHCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCH
TAFKACKKYLAK
HHHHHHHHHHCC
>Mature Secondary Structure 
AVIESVYARQILDSRGNPTVEVYLETEDGAQGKGLVPSGASTGEAEAWERRDGDKSVYG
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GKGVLNAVKAVNEVIAPKVIGMDAADQRALDDLMIELDGTPNKGKLGANAILGVSLAALY
CHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHH
ASAESAGLPLYRYIGGTNGHILPVPNMNIMNGGAHADFATDIQEYMISPYGFDTYSEALR
HHHCCCCCCEEEECCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
AGVEVYHTLKNVLKKEGLNTGLGDEGGFAPKMKSNEDSLKYIMDAISAAGYEPGKQIGIC
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSEFYNKETGKYRFDGEERDSAYMLDYYENLINEYPIVSIEDPFNEEGWEDWAAIT
EEECCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
ARLGDRLQFVGDDLLVTNPARLQKAIDLGAANSLLVKLNQIGSVTETLDAIELATANGYT
HHHCCHHHHCCCCEEEECHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
SMVSHRSGETPDTTISDLAVAKNTRQIKTGAPARGERVAKYNRLLEIEEELGSTAQYAGY
HHHHCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCH
TAFKACKKYLAK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA