Click here to switch to the map view.

The map label for this gene is ackA [H]

Identifier: 213692598

GI number: 213692598

Start: 1900148

End: 1901377

Strand: Direct

Name: ackA [H]

Synonym: Blon_1731

Alternate gene names: 213692598

Gene position: 1900148-1901377 (Clockwise)

Preceding gene: 213692597

Following gene: 213692602

Centisome position: 67.08

GC content: 64.07

Gene sequence:

>1230_bases
ATGGCGAAAACCGTCCTTGTCATCAATTCCGGCTCCAGCTCGATCAAGTACCAGCTGGTTGATCTTGAATCCGGCGAAGG
CCTCGCTTCCGGTCTTGTCGAGAAGATCGGCGAACCGGTCGACGGCCACTACAAGCACGAGTACAACGGCGAAAAGCATG
AGCTTGAAGAGCCGATTCACGACCACGAGCAGGGTCTCAAGCGCGTGCTCGGCTTCTTCGAGGAGTACGGCCCGAAGCTG
TCCGAGGCCGGCATCGTCGCCGTCGGCCACCGCGTGGTGCAGGGTGGCTCCATCTTCCCGAACCCGGCCCTCGTGACCGA
CAAGACCATTAACCAGGTCAAGGATCTGGCCGTGCTCGCCCCGCTGCACAACGGCCCGGAGGCCAAGGGCGCCGAGGTCA
TGCGTTCCCTGCTGCCGGATGTTCCGCAGATCTTCGTGTTCGACTCCTCCTTCTTCTTCCAGCTGCCGAAGGCCGCCAGC
ACCTACGCGCTGAACAAGGAGATCGCCGACCAGTACCACATCCGCCGCTACGGCGCCCACGGCACCTCCCACGAATACAT
CTCCTCCGTGGTGCCGGGCGTCATCGGCAAGCCGGCCGAAGGCCTCAAGCAGATCGTCCTGCACATCGGCAACGGCGCCT
CCGCCTCCGCCGAGATCTCCGGCAAGCCGGTCGAGACCTCCATGGGCCTGACCCCGCTTGAGGGCCTGATGATGGGCGGC
CGCACCGGCGACATCGACCCGGCCGTGGTCTTCCACCTGATCCGCAACGCCCACATGAACGTGGACGAGCTCGACGCCCT
GTTCAACAAGCGTTCCGGCATGATGGGCATGACCGGCTACGGCGACCTGCGCGAGGTCCACCGTCTGGTGTCCGAGGGCA
ACGAGGACGCCAAGCTGGCCCTCGATGTCTACGTGCACCGTATCGTCAGCTACATCGGCAACTACACCTACCAGATGGGC
GGCTGCGATGTGATCACCTTCACCGCCGGTGTGGGCGAGAACGACGATGTGGTCCGCAAGATGGTGTGCGACAAGCTCGC
TCCGTTCGGCGTCAAGCTGGACGAGGCGAAGAACGCCGTGCGCTCCAAGGAGCCGCGCATCATCTCCACCCCGGACTCCT
CCGTGGTCATCGCCGTGATTCCGACGAACGAGGAGCTGGCCATCGCCCGCAAGTCCGCCGCGATCGCCGAAGCCGGCACC
GACACCTACGGCAACACCTTCGCCAAGTAA

Upstream 100 bases:

>100_bases
GAGCCACTGTAATGCTTATTTTGTAGCAAGCAACGAGTAATCTAGTCAACGGTAACCCGTGTCTCACGGGAACATTCAAT
CACAATGGAGGATGCCCACA

Downstream 100 bases:

>100_bases
GTAACCGCCGTTAGGCATCTCACTAATCTCTAGTTGCAACACTATGGGGGGCTGACTCCGATTGGAATCAGCCCCCCATT
CGTATATGGTCATGAGACCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAKTVLVINSGSSSIKYQLVDLESGEGLASGLVEKIGEPVDGHYKHEYNGEKHELEEPIHDHEQGLKRVLGFFEEYGPKL
SEAGIVAVGHRVVQGGSIFPNPALVTDKTINQVKDLAVLAPLHNGPEAKGAEVMRSLLPDVPQIFVFDSSFFFQLPKAAS
TYALNKEIADQYHIRRYGAHGTSHEYISSVVPGVIGKPAEGLKQIVLHIGNGASASAEISGKPVETSMGLTPLEGLMMGG
RTGDIDPAVVFHLIRNAHMNVDELDALFNKRSGMMGMTGYGDLREVHRLVSEGNEDAKLALDVYVHRIVSYIGNYTYQMG
GCDVITFTAGVGENDDVVRKMVCDKLAPFGVKLDEAKNAVRSKEPRIISTPDSSVVIAVIPTNEELAIARKSAAIAEAGT
DTYGNTFAK

Sequences:

>Translated_409_residues
MAKTVLVINSGSSSIKYQLVDLESGEGLASGLVEKIGEPVDGHYKHEYNGEKHELEEPIHDHEQGLKRVLGFFEEYGPKL
SEAGIVAVGHRVVQGGSIFPNPALVTDKTINQVKDLAVLAPLHNGPEAKGAEVMRSLLPDVPQIFVFDSSFFFQLPKAAS
TYALNKEIADQYHIRRYGAHGTSHEYISSVVPGVIGKPAEGLKQIVLHIGNGASASAEISGKPVETSMGLTPLEGLMMGG
RTGDIDPAVVFHLIRNAHMNVDELDALFNKRSGMMGMTGYGDLREVHRLVSEGNEDAKLALDVYVHRIVSYIGNYTYQMG
GCDVITFTAGVGENDDVVRKMVCDKLAPFGVKLDEAKNAVRSKEPRIISTPDSSVVIAVIPTNEELAIARKSAAIAEAGT
DTYGNTFAK
>Mature_408_residues
AKTVLVINSGSSSIKYQLVDLESGEGLASGLVEKIGEPVDGHYKHEYNGEKHELEEPIHDHEQGLKRVLGFFEEYGPKLS
EAGIVAVGHRVVQGGSIFPNPALVTDKTINQVKDLAVLAPLHNGPEAKGAEVMRSLLPDVPQIFVFDSSFFFQLPKAAST
YALNKEIADQYHIRRYGAHGTSHEYISSVVPGVIGKPAEGLKQIVLHIGNGASASAEISGKPVETSMGLTPLEGLMMGGR
TGDIDPAVVFHLIRNAHMNVDELDALFNKRSGMMGMTGYGDLREVHRLVSEGNEDAKLALDVYVHRIVSYIGNYTYQMGG
CDVITFTAGVGENDDVVRKMVCDKLAPFGVKLDEAKNAVRSKEPRIISTPDSSVVIAVIPTNEELAIARKSAAIAEAGTD
TYGNTFAK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=40.25, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI145693184, Length=387, Percent_Identity=38.5012919896641, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 44084; Mature: 43952

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2 ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVLVINSGSSSIKYQLVDLESGEGLASGLVEKIGEPVDGHYKHEYNGEKHELEEPIH
CCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHH
DHEQGLKRVLGFFEEYGPKLSEAGIVAVGHRVVQGGSIFPNPALVTDKTINQVKDLAVLA
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCEECHHHHHHHHHHHHEE
PLHNGPEAKGAEVMRSLLPDVPQIFVFDSSFFFQLPKAASTYALNKEIADQYHIRRYGAH
CCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHCCC
GTSHEYISSVVPGVIGKPAEGLKQIVLHIGNGASASAEISGKPVETSMGLTPLEGLMMGG
CCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
RTGDIDPAVVFHLIRNAHMNVDELDALFNKRSGMMGMTGYGDLREVHRLVSEGNEDAKLA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
LDVYVHRIVSYIGNYTYQMGGCDVITFTAGVGENDDVVRKMVCDKLAPFGVKLDEAKNAV
HHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RSKEPRIISTPDSSVVIAVIPTNEELAIARKSAAIAEAGTDTYGNTFAK
HCCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AKTVLVINSGSSSIKYQLVDLESGEGLASGLVEKIGEPVDGHYKHEYNGEKHELEEPIH
CCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHH
DHEQGLKRVLGFFEEYGPKLSEAGIVAVGHRVVQGGSIFPNPALVTDKTINQVKDLAVLA
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCEECHHHHHHHHHHHHEE
PLHNGPEAKGAEVMRSLLPDVPQIFVFDSSFFFQLPKAASTYALNKEIADQYHIRRYGAH
CCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHCCC
GTSHEYISSVVPGVIGKPAEGLKQIVLHIGNGASASAEISGKPVETSMGLTPLEGLMMGG
CCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
RTGDIDPAVVFHLIRNAHMNVDELDALFNKRSGMMGMTGYGDLREVHRLVSEGNEDAKLA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
LDVYVHRIVSYIGNYTYQMGGCDVITFTAGVGENDDVVRKMVCDKLAPFGVKLDEAKNAV
HHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RSKEPRIISTPDSSVVIAVIPTNEELAIARKSAAIAEAGTDTYGNTFAK
HCCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA