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The map label for this gene is nhaA [H]

Identifier: 213692573

GI number: 213692573

Start: 1865818

End: 1867254

Strand: Reverse

Name: nhaA [H]

Synonym: Blon_1705

Alternate gene names: 213692573

Gene position: 1867254-1865818 (Counterclockwise)

Preceding gene: 213692574

Following gene: 213692572

Centisome position: 65.92

GC content: 63.6

Gene sequence:

>1437_bases
ATGGCCACGACCGCCGGCGCGAAGAAGGGACTTTGGTCCACCATCAGGCGAATCGCCGCCTCAGACCGTATTTCCGGACT
CATCATGCTCGGCTTCGCGCTCACCGGCCTGGTGCTCGCGAACCTGCCCGCCACCGCGCATGCGTTCGAGACCGTAGCGG
AAACGCATCTGTTCATCCCCTACACCAATCTCGACCTGCCGATTGGCCATTGGGCCCAGGATGGTCTGCTTACCATCTTC
TTCCTGACCGTGGGCCTCGAACTGAAGCAGGAACTCACCACCGGCTCGCTCGCCAATCCGAAAGCGGCCGCCGTGCCGAT
GCTGTGCGCGGTGGGCGGCATGATCGCCCCGCCCATCCTGTTCCTGGCCGTCACGGCATTGTTCTCTCAGATCGGGCCTG
GTGAACCCGGCACGCTGATTCTGACCACCACCGGCAGCAGCATCCCGTTCTCGGAGATGTCGCACGGCTGGGCAGTGCCC
ACCGCCACGGATATCGCGTTCTCACTGGCCGTGCTCGCCCTGTTTGCCAAGGCCCTACCCGGTTCGATCCGCGCGTTCCT
GATGACGCTTGCCACCGTCGATGACCTGCTGGCCATTATTTTGATCGCCGTATTCTTCTCCTCGATCAACGCCTGGTATT
GGTTCATCGGCATCGTCGTGTGCGCCGTCATCTGGGCATACCTCGTACGACTGAAAAAAGTGCCGTGGATCGCGGTCGGC
ATCGTCGGCGTCCTCGCTTGGATCATGATGTTCGAGGCAGGCGTGCATCCCACACTGGCCGGCGTGCTGGTTGGCCTGCT
GACCCCCTCCCGTGAAATGCACGGCGAACTTTCACCGCGTGCCGAACGCTATGCCAACAAGCTTCAGCCATTCTCCGCAC
TGCTGGCCCTGCCCATCTTCGCCCTGTTCGCCACCGGCGTGCACTTCGAGTCGGTGAGCCCGCTGTTGTTGGCTTCGCCG
TTGGTCATTGCACTGATTGTGGCTTTGGTGGTCGGCAAACCTCTGGGCATCATCACCACCGCATGGCTGGCCACGCATGT
GGGCGGGCTCAAGATGGCCAAGGGGCTGCGCGTGCGCGACATGATTCCCGCTGCCGTCGCCTGCGGCATTGGCTTCACTG
TGTCCTTCCTCATCGCCTCGCTGGCGTACAAGAACGCGGAACTTTCCGCTGAGGCGCGCTTCGGCGTGCTGGTGGCCTCC
CTGATCGCCGCCGCGATTTCCGGTGTGCTGCTGAGCCGTCAGTCCAAGAGATTCGAGAAGGCCGCAGCCGCGCAGGCTGC
CGCCGCCGAGGCGTTGGCCGATGCCGAAGCCGGCGAATCGATTGATGGCAATGGCACCGGGCAGCCAAGCCGCACCACAA
AGCCCACCACGCCCACGGAACACCCCGGCACCCTCGCCGACGGCACCGCCAGTGTGGAAATCGACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
GGTAAGGTTGCCCTTCGCGACTCAGCCCGGAGCGGAACGTCCTGAGATATTCCGTACTATTACATACGGTTCATCCAAGA
GAGTACAGCAAGGAGACGCG

Downstream 100 bases:

>100_bases
TGGCTAAGACGGTCCCTACTCCGTTGTTGGCCTTGTTTCGCGGAAAACGTACGCAGAGCTTCAGGCGCTAGAATGCGAAG
AGTCGATAGCACCGTACAGG

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MATTAGAKKGLWSTIRRIAASDRISGLIMLGFALTGLVLANLPATAHAFETVAETHLFIPYTNLDLPIGHWAQDGLLTIF
FLTVGLELKQELTTGSLANPKAAAVPMLCAVGGMIAPPILFLAVTALFSQIGPGEPGTLILTTTGSSIPFSEMSHGWAVP
TATDIAFSLAVLALFAKALPGSIRAFLMTLATVDDLLAIILIAVFFSSINAWYWFIGIVVCAVIWAYLVRLKKVPWIAVG
IVGVLAWIMMFEAGVHPTLAGVLVGLLTPSREMHGELSPRAERYANKLQPFSALLALPIFALFATGVHFESVSPLLLASP
LVIALIVALVVGKPLGIITTAWLATHVGGLKMAKGLRVRDMIPAAVACGIGFTVSFLIASLAYKNAELSAEARFGVLVAS
LIAAAISGVLLSRQSKRFEKAAAAQAAAAEALADAEAGESIDGNGTGQPSRTTKPTTPTEHPGTLADGTASVEIDFRH

Sequences:

>Translated_478_residues
MATTAGAKKGLWSTIRRIAASDRISGLIMLGFALTGLVLANLPATAHAFETVAETHLFIPYTNLDLPIGHWAQDGLLTIF
FLTVGLELKQELTTGSLANPKAAAVPMLCAVGGMIAPPILFLAVTALFSQIGPGEPGTLILTTTGSSIPFSEMSHGWAVP
TATDIAFSLAVLALFAKALPGSIRAFLMTLATVDDLLAIILIAVFFSSINAWYWFIGIVVCAVIWAYLVRLKKVPWIAVG
IVGVLAWIMMFEAGVHPTLAGVLVGLLTPSREMHGELSPRAERYANKLQPFSALLALPIFALFATGVHFESVSPLLLASP
LVIALIVALVVGKPLGIITTAWLATHVGGLKMAKGLRVRDMIPAAVACGIGFTVSFLIASLAYKNAELSAEARFGVLVAS
LIAAAISGVLLSRQSKRFEKAAAAQAAAAEALADAEAGESIDGNGTGQPSRTTKPTTPTEHPGTLADGTASVEIDFRH
>Mature_477_residues
ATTAGAKKGLWSTIRRIAASDRISGLIMLGFALTGLVLANLPATAHAFETVAETHLFIPYTNLDLPIGHWAQDGLLTIFF
LTVGLELKQELTTGSLANPKAAAVPMLCAVGGMIAPPILFLAVTALFSQIGPGEPGTLILTTTGSSIPFSEMSHGWAVPT
ATDIAFSLAVLALFAKALPGSIRAFLMTLATVDDLLAIILIAVFFSSINAWYWFIGIVVCAVIWAYLVRLKKVPWIAVGI
VGVLAWIMMFEAGVHPTLAGVLVGLLTPSREMHGELSPRAERYANKLQPFSALLALPIFALFATGVHFESVSPLLLASPL
VIALIVALVVGKPLGIITTAWLATHVGGLKMAKGLRVRDMIPAAVACGIGFTVSFLIASLAYKNAELSAEARFGVLVASL
IAAAISGVLLSRQSKRFEKAAAAQAAAAEALADAEAGESIDGNGTGQPSRTTKPTTPTEHPGTLADGTASVEIDFRH

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family [H]

Homologues:

Organism=Escherichia coli, GI1786201, Length=397, Percent_Identity=37.27959697733, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023171
- InterPro:   IPR004670 [H]

Pfam domain/function: PF06965 Na_H_antiport_1 [H]

EC number: NA

Molecular weight: Translated: 50006; Mature: 49875

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTAGAKKGLWSTIRRIAASDRISGLIMLGFALTGLVLANLPATAHAFETVAETHLFIP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEE
YTNLDLPIGHWAQDGLLTIFFLTVGLELKQELTTGSLANPKAAAVPMLCAVGGMIAPPIL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHH
FLAVTALFSQIGPGEPGTLILTTTGSSIPFSEMSHGWAVPTATDIAFSLAVLALFAKALP
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GSIRAFLMTLATVDDLLAIILIAVFFSSINAWYWFIGIVVCAVIWAYLVRLKKVPWIAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IVGVLAWIMMFEAGVHPTLAGVLVGLLTPSREMHGELSPRAERYANKLQPFSALLALPIF
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFATGVHFESVSPLLLASPLVIALIVALVVGKPLGIITTAWLATHVGGLKMAKGLRVRD
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHH
MIPAAVACGIGFTVSFLIASLAYKNAELSAEARFGVLVASLIAAAISGVLLSRQSKRFEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAQAAAAEALADAEAGESIDGNGTGQPSRTTKPTTPTEHPGTLADGTASVEIDFRH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
ATTAGAKKGLWSTIRRIAASDRISGLIMLGFALTGLVLANLPATAHAFETVAETHLFIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEE
YTNLDLPIGHWAQDGLLTIFFLTVGLELKQELTTGSLANPKAAAVPMLCAVGGMIAPPIL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHH
FLAVTALFSQIGPGEPGTLILTTTGSSIPFSEMSHGWAVPTATDIAFSLAVLALFAKALP
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GSIRAFLMTLATVDDLLAIILIAVFFSSINAWYWFIGIVVCAVIWAYLVRLKKVPWIAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IVGVLAWIMMFEAGVHPTLAGVLVGLLTPSREMHGELSPRAERYANKLQPFSALLALPIF
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFATGVHFESVSPLLLASPLVIALIVALVVGKPLGIITTAWLATHVGGLKMAKGLRVRD
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHH
MIPAAVACGIGFTVSFLIASLAYKNAELSAEARFGVLVASLIAAAISGVLLSRQSKRFEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAQAAAAEALADAEAGESIDGNGTGQPSRTTKPTTPTEHPGTLADGTASVEIDFRH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]