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The map label for this gene is mdeA [H]

Identifier: 213692564

GI number: 213692564

Start: 1854288

End: 1855604

Strand: Reverse

Name: mdeA [H]

Synonym: Blon_1696

Alternate gene names: 213692564

Gene position: 1855604-1854288 (Counterclockwise)

Preceding gene: 213692572

Following gene: 213692563

Centisome position: 65.51

GC content: 62.57

Gene sequence:

>1317_bases
ATGGTCGAGAACAACAAGAAGTACCGTTTTGAAACCCTGCAGCTGCATGTGGGCCAGGAGCAGGCCGACCCGGCCACCGA
TTCCCGCGCAGTGCCGATCTACGCCACCACCTCCTACGTCTTCCACAACTTCGATCATGCCGAGGCCCGATTCGGCCTTG
CCGACCCGGGCAACATCTACGGCCGTCTGACCAACACCACCCAGGGTGTCTTTGAGGACCGCATCGCCGCCCTTGAAGGT
GGTACCGCAGGCCTTGCCGTCGCCTCCGGTGCAGCCGCCGTGGAATACGCGGTGCGCAACATCACCCAGTCCGGTGATCA
CATCGTCGCCGCCAAGAACATCTACGGCGGCACCTTCAACCTGCTGCGCCACACCCTGCCGCGCGACGGCATCACCACCA
CCTTCGTCTCCGCCGAGAACCCGCAGGAGTTCGAGGATGCCATTCAGGAGAACACCAAGCTCGTCTACTTCGAGACCTTC
GGCAACCCGAACGCTGATCTGCCGGATTTCGAGGCAATCTCCGAAATCGCCCACAAGCACAACCTGCCGGTGATCGTGGA
CAACACCTTCGCCACCCCGTACCTGTTCCGCCCGCTGGAGCACGGCGCCGACGTGGTGGTTGAATCCGCCACCAAGTTCA
TCGGCGGCCACGGCACCACTTTGGGTGGTGTGATTGTTGAAGGCGGCAACTTCGACTGGAATGCGGTTCCGGGCAAGTTC
CCGACCCTGACCGAGCCGGACCCCTCCTACCATGGCCTGAACTTCTACGAGGCTCTCGGCGGCACCGCCTTCGTGACCCG
TATCCGCGCCATCCTGCTGCGCGATACCGGTGCCACCCTGTCCCCGTTCGCCGCCTTCCTGCTGCTGCAGGGCACCGAAA
CCCTGTCCCTGCGTGTTGAGCGCCACGTCCAGAACGCGCTGAAGGTCGTCGAGTACCTGCAGACCGTGCCGGAAGTCGAG
TCCGTTTCCCACCCGTCCATCCCGGGCCGTCCGGATCACGACCTGTACGAGAAGTACTTCCCGAACGGAGCAGGCTCCAT
CTTCACCTTCGATATCAAGGGCGGAAAGGATGCGGCTCGCGTCTTCATCGACAATCTGCACCTGTTCTCTCTGCTTGCCA
ATGTGGCCGACGCAAAGTCCCTCGTCATCCACCCGGCTTCCACCACGCACTCCCAGGAGACCTTGGAAGAGCTCGAGGAC
CAGGGCATTCACCAGGGCACCATCCGCCTGTCCATCGGCACCGAGAACATCGAGGACATCCTCGATGACCTGAAGGGCGG
CTTCGAAGCCCTTCGCGCTTCCGGCTTGGCCAAGTGA

Upstream 100 bases:

>100_bases
GTCGTAACTTCATAACCAACACCTCGCCAACCACCCGAACACGGCAGGCGGGCGGCAAGGAAAAATCCTCGCGACCTAGC
ATAACCAAGGAGAAATACCC

Downstream 100 bases:

>100_bases
GGTCGACAGAAAGGTTGACAGCCCGATGAGAAGGGGCGCGATGCTAATGGGGTGCATCGCGAATCATCGAGCCGCATGGG
GTGCCTTCCGCCTGATCGCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIYGRLTNTTQGVFEDRIAALEG
GTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFNLLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETF
GNPNADLPDFEAISEIAHKHNLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGNFDWNAVPGKF
PTLTEPDPSYHGLNFYEALGGTAFVTRIRAILLRDTGATLSPFAAFLLLQGTETLSLRVERHVQNALKVVEYLQTVPEVE
SVSHPSIPGRPDHDLYEKYFPNGAGSIFTFDIKGGKDAARVFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELED
QGIHQGTIRLSIGTENIEDILDDLKGGFEALRASGLAK

Sequences:

>Translated_438_residues
MVENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIYGRLTNTTQGVFEDRIAALEG
GTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFNLLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETF
GNPNADLPDFEAISEIAHKHNLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGNFDWNAVPGKF
PTLTEPDPSYHGLNFYEALGGTAFVTRIRAILLRDTGATLSPFAAFLLLQGTETLSLRVERHVQNALKVVEYLQTVPEVE
SVSHPSIPGRPDHDLYEKYFPNGAGSIFTFDIKGGKDAARVFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELED
QGIHQGTIRLSIGTENIEDILDDLKGGFEALRASGLAK
>Mature_438_residues
MVENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIYGRLTNTTQGVFEDRIAALEG
GTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFNLLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETF
GNPNADLPDFEAISEIAHKHNLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGNFDWNAVPGKF
PTLTEPDPSYHGLNFYEALGGTAFVTRIRAILLRDTGATLSPFAAFLLLQGTETLSLRVERHVQNALKVVEYLQTVPEVE
SVSHPSIPGRPDHDLYEKYFPNGAGSIFTFDIKGGKDAARVFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELED
QGIHQGTIRLSIGTENIEDILDDLKGGFEALRASGLAK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=409, Percent_Identity=31.2958435207824, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI299473759, Length=410, Percent_Identity=28.2926829268293, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI25453487, Length=409, Percent_Identity=26.6503667481663, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1790375, Length=399, Percent_Identity=30.3258145363409, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789383, Length=377, Percent_Identity=26.790450928382, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=433, Percent_Identity=32.3325635103926, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=410, Percent_Identity=32.6829268292683, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=409, Percent_Identity=32.0293398533007, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=49.0825688073394, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319307, Length=376, Percent_Identity=32.1808510638298, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=26.4044943820225, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321254, Length=440, Percent_Identity=25.2272727272727, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=23.8979118329466, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=33.5697399527187, Blast_Score=209, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47622; Mature: 47622

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIY
CCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHCCCCCHHHCCCCCCCCCEE
GRLTNTTQGVFEDRIAALEGGTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFN
EEECCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
LLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETFGNPNADLPDFEAISEIAHKH
HHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHC
NLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGNFDWNAVPGKF
CCCEEEECCCCCCHHHCHHHCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCC
PTLTEPDPSYHGLNFYEALGGTAFVTRIRAILLRDTGATLSPFAAFLLLQGTETLSLRVE
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEHHHH
RHVQNALKVVEYLQTVPEVESVSHPSIPGRPDHDLYEKYFPNGAGSIFTFDIKGGKDAAR
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHH
VFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELEDQGIHQGTIRLSIGTENIEDI
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
LDDLKGGFEALRASGLAK
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIY
CCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHCCCCCHHHCCCCCCCCCEE
GRLTNTTQGVFEDRIAALEGGTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFN
EEECCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
LLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETFGNPNADLPDFEAISEIAHKH
HHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHC
NLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGNFDWNAVPGKF
CCCEEEECCCCCCHHHCHHHCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCC
PTLTEPDPSYHGLNFYEALGGTAFVTRIRAILLRDTGATLSPFAAFLLLQGTETLSLRVE
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEHHHH
RHVQNALKVVEYLQTVPEVESVSHPSIPGRPDHDLYEKYFPNGAGSIFTFDIKGGKDAAR
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHH
VFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELEDQGIHQGTIRLSIGTENIEDI
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
LDDLKGGFEALRASGLAK
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]