Click here to switch to the map view.

The map label for this gene is rob [H]

Identifier: 213692556

GI number: 213692556

Start: 1841907

End: 1842767

Strand: Direct

Name: rob [H]

Synonym: Blon_1688

Alternate gene names: 213692556

Gene position: 1841907-1842767 (Clockwise)

Preceding gene: 213692555

Following gene: 213692560

Centisome position: 65.02

GC content: 44.72

Gene sequence:

>861_bases
TTGAAACGATATCCCCCTTTAGAAAGGGCCATTACCTATATCGAAGCACATCTAAATGAATATATCGGTCTGAATGAAGT
ATCAAGAGAAACCGGCTACTCCTACTACTACATGACCCGCCTGTTTTCTTCTGTTTTAGGTGAATCCGTGGGACACTATA
TCAACCGTCGGAGGCTTTACAATGCGTCCGAAAAACTGATTCATTCCGACCAGAAGGTCATTGATATTGCACTTGACTGT
GGTTATGAATCGTCAGAGGCGTTCAGCAGGGCCTTTAAAGCTGTTTTTGGATACAGTCCTGTAGCTTACCGGAAAGCCGG
ACTTGATTTGGTGATAAAAGCAAAAAAAGAACTGGCGCCGGAAGATGTGTGCCATATTGCCAATCATATTTCACACGCTC
CCAAGATCATCCTGCTAGGTGAGACAGAAGTAGCTGGGCTTCGGGGGACTACTTCCCTGTCCGACAATCAGCTGCCCGGA
CTGTGGGAACAGTTCCATGGTCTTAACAAAGATTTTTTCACCACATCTGCGGGATATGGCATCTGTGAAACGCAGAAGAC
GGCTTATACAAAAGACGGCGACGCGTTATATTCCGTCATGATCGGCGGTCCGGTGAAGGATTTTGATTGCCTGCCGCTGG
AACTGGCAAAAAAGACGCTGCGTGCAGGAAGATACGCTGTTTTCACCCACCGGGGCACACTTGGAAACTTATATAAAACA
TATCAGTATATCTTCGGAACATGGTTGCAGACCACTAGGGAAGAACTGGATGACAGGGAAGATTTTGAAGTTTATGAGCA
TGAAATTCTATCCTTTGATGATCCAGATAATGAAGTTAAAATTTTTATTCCTATCAAATGA

Upstream 100 bases:

>100_bases
CATAAAAAAAAACTTTGTTGCCAAACGAAAGAAAAGAAGCATTAAGAGAATTTTATAACAGATATAAAAAGTAATAGTAA
AAACGGATGGAGGTGCCTGT

Downstream 100 bases:

>100_bases
CAGGACACCATTTTGCGCACGGGCGCTGACGGTGTTTTACATATCACCGGGTATTTAGGGTGTGTTTACACTATCTAGGT
TTTGTGTGATAATTATCGTA

Product: transcription activator, effector binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKRYPPLERAITYIEAHLNEYIGLNEVSRETGYSYYYMTRLFSSVLGESVGHYINRRRLYNASEKLIHSDQKVIDIALDC
GYESSEAFSRAFKAVFGYSPVAYRKAGLDLVIKAKKELAPEDVCHIANHISHAPKIILLGETEVAGLRGTTSLSDNQLPG
LWEQFHGLNKDFFTTSAGYGICETQKTAYTKDGDALYSVMIGGPVKDFDCLPLELAKKTLRAGRYAVFTHRGTLGNLYKT
YQYIFGTWLQTTREELDDREDFEVYEHEILSFDDPDNEVKIFIPIK

Sequences:

>Translated_286_residues
MKRYPPLERAITYIEAHLNEYIGLNEVSRETGYSYYYMTRLFSSVLGESVGHYINRRRLYNASEKLIHSDQKVIDIALDC
GYESSEAFSRAFKAVFGYSPVAYRKAGLDLVIKAKKELAPEDVCHIANHISHAPKIILLGETEVAGLRGTTSLSDNQLPG
LWEQFHGLNKDFFTTSAGYGICETQKTAYTKDGDALYSVMIGGPVKDFDCLPLELAKKTLRAGRYAVFTHRGTLGNLYKT
YQYIFGTWLQTTREELDDREDFEVYEHEILSFDDPDNEVKIFIPIK
>Mature_286_residues
MKRYPPLERAITYIEAHLNEYIGLNEVSRETGYSYYYMTRLFSSVLGESVGHYINRRRLYNASEKLIHSDQKVIDIALDC
GYESSEAFSRAFKAVFGYSPVAYRKAGLDLVIKAKKELAPEDVCHIANHISHAPKIILLGETEVAGLRGTTSLSDNQLPG
LWEQFHGLNKDFFTTSAGYGICETQKTAYTKDGDALYSVMIGGPVKDFDCLPLELAKKTLRAGRYAVFTHRGTLGNLYKT
YQYIFGTWLQTTREELDDREDFEVYEHEILSFDDPDNEVKIFIPIK

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=293, Percent_Identity=25.2559726962457, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=34.6938775510204, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081928, Length=99, Percent_Identity=31.3131313131313, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32577; Mature: 32577

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYPPLERAITYIEAHLNEYIGLNEVSRETGYSYYYMTRLFSSVLGESVGHYINRRRLY
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NASEKLIHSDQKVIDIALDCGYESSEAFSRAFKAVFGYSPVAYRKAGLDLVIKAKKELAP
CHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEECHHHCCH
EDVCHIANHISHAPKIILLGETEVAGLRGTTSLSDNQLPGLWEQFHGLNKDFFTTSAGYG
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCC
ICETQKTAYTKDGDALYSVMIGGPVKDFDCLPLELAKKTLRAGRYAVFTHRGTLGNLYKT
EECCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHH
YQYIFGTWLQTTREELDDREDFEVYEHEILSFDDPDNEVKIFIPIK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKRYPPLERAITYIEAHLNEYIGLNEVSRETGYSYYYMTRLFSSVLGESVGHYINRRRLY
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NASEKLIHSDQKVIDIALDCGYESSEAFSRAFKAVFGYSPVAYRKAGLDLVIKAKKELAP
CHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEECHHHCCH
EDVCHIANHISHAPKIILLGETEVAGLRGTTSLSDNQLPGLWEQFHGLNKDFFTTSAGYG
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCC
ICETQKTAYTKDGDALYSVMIGGPVKDFDCLPLELAKKTLRAGRYAVFTHRGTLGNLYKT
EECCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHH
YQYIFGTWLQTTREELDDREDFEVYEHEILSFDDPDNEVKIFIPIK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]