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The map label for this gene is pncA [H]

Identifier: 213692493

GI number: 213692493

Start: 1773927

End: 1774619

Strand: Direct

Name: pncA [H]

Synonym: Blon_1622

Alternate gene names: 213692493

Gene position: 1773927-1774619 (Clockwise)

Preceding gene: 213692491

Following gene: 213692494

Centisome position: 62.62

GC content: 60.75

Gene sequence:

>693_bases
ATGGCTATGATGAGAGCAATCGCACGTCGAGTTCGAGACAATATTCAAAACGAATCCAAGGCAATAGGGGGAATGACGAT
GACCAAGGCACTGATTGTGGTGGATGTGCAGCCGACGTTCTGTGAAGGCGGCGAACTGGGAGTCGAAGGCGGCAACGCGG
TCGCCGAGCGGATCGCCGATTATGTGAACGCGCATCGCAGCGAGTACGCGTATATCGCAACCACGCAGGACTGGCATATC
GAGCCGGGCGCGCACTGGTCGGCGGAACCGGATTTCGTGGACACGTGGCCGAAGCATGGTGCGGCCGGCACGCCGAACGC
CGAATTGCATACGGCGATCAAGGCGCTGAATATCCAGCATCATTTCAAGAAGGGCCAGTATTCGGCGGCATATTCCGGTT
TTGAGGGCATCGAGGACAATACCGACCGTATCCAGACCCGCGAGGAGGTCACGGCCGAGCAATCCGCCGGCAAGACGCTG
GCCAATGCGCTGAAGGCCGCCGGCGTGAGTCGCGTGGATGTGGTGGGCATCGCCGAATCGCATTGCGTCAAGGACACCGC
GCTCGATGCCGGGAAGCTTGGTTTCGAGGTCACCGTGTTCGAGGGCCTGACCGTACCGGTCAGCGAGGAGCTCGGTGTCG
CTGCCCGCAAACAGATGACGGCGGCCAGCATCACATTGGCCGAGTCCCGCTGA

Upstream 100 bases:

>100_bases
ACGTCAGATGGTCCAGCATAGTCTTGGTCGCCCTCCCCTGGTTCTTGTATTCGATTGCTGCTTGCAGTGTAAGCCCGAAC
GTTTCCCCACAGGGCCGCGC

Downstream 100 bases:

>100_bases
TCAGGCCGGATATACCCAGAGCGAACGCACGAACACTTCCGTATATTCAAGCCCGACACTGCCGCATAGTCGCCTAGTCG
ATATGTGAAGAAGGCTTTTA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MAMMRAIARRVRDNIQNESKAIGGMTMTKALIVVDVQPTFCEGGELGVEGGNAVAERIADYVNAHRSEYAYIATTQDWHI
EPGAHWSAEPDFVDTWPKHGAAGTPNAELHTAIKALNIQHHFKKGQYSAAYSGFEGIEDNTDRIQTREEVTAEQSAGKTL
ANALKAAGVSRVDVVGIAESHCVKDTALDAGKLGFEVTVFEGLTVPVSEELGVAARKQMTAASITLAESR

Sequences:

>Translated_230_residues
MAMMRAIARRVRDNIQNESKAIGGMTMTKALIVVDVQPTFCEGGELGVEGGNAVAERIADYVNAHRSEYAYIATTQDWHI
EPGAHWSAEPDFVDTWPKHGAAGTPNAELHTAIKALNIQHHFKKGQYSAAYSGFEGIEDNTDRIQTREEVTAEQSAGKTL
ANALKAAGVSRVDVVGIAESHCVKDTALDAGKLGFEVTVFEGLTVPVSEELGVAARKQMTAASITLAESR
>Mature_229_residues
AMMRAIARRVRDNIQNESKAIGGMTMTKALIVVDVQPTFCEGGELGVEGGNAVAERIADYVNAHRSEYAYIATTQDWHIE
PGAHWSAEPDFVDTWPKHGAAGTPNAELHTAIKALNIQHHFKKGQYSAAYSGFEGIEDNTDRIQTREEVTAEQSAGKTLA
NALKAAGVSRVDVVGIAESHCVKDTALDAGKLGFEVTVFEGLTVPVSEELGVAARKQMTAASITLAESR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=217, Percent_Identity=31.3364055299539, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 24697; Mature: 24566

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMMRAIARRVRDNIQNESKAIGGMTMTKALIVVDVQPTFCEGGELGVEGGNAVAERIAD
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
YVNAHRSEYAYIATTQDWHIEPGAHWSAEPDFVDTWPKHGAAGTPNAELHTAIKALNIQH
HHHHHCCCEEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
HFKKGQYSAAYSGFEGIEDNTDRIQTREEVTAEQSAGKTLANALKAAGVSRVDVVGIAES
HHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCHH
HCVKDTALDAGKLGFEVTVFEGLTVPVSEELGVAARKQMTAASITLAESR
HHHHHHCCCCCCCCEEEEEECCCCCCCCHHHCHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
AMMRAIARRVRDNIQNESKAIGGMTMTKALIVVDVQPTFCEGGELGVEGGNAVAERIAD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
YVNAHRSEYAYIATTQDWHIEPGAHWSAEPDFVDTWPKHGAAGTPNAELHTAIKALNIQH
HHHHHCCCEEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
HFKKGQYSAAYSGFEGIEDNTDRIQTREEVTAEQSAGKTLANALKAAGVSRVDVVGIAES
HHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCHH
HCVKDTALDAGKLGFEVTVFEGLTVPVSEELGVAARKQMTAASITLAESR
HHHHHHCCCCCCCCEEEEEECCCCCCCCHHHCHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]