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The map label for this gene is pyrG [H]

Identifier: 213692466

GI number: 213692466

Start: 1747318

End: 1748997

Strand: Reverse

Name: pyrG [H]

Synonym: Blon_1595

Alternate gene names: 213692466

Gene position: 1748997-1747318 (Counterclockwise)

Preceding gene: 213692467

Following gene: 213692465

Centisome position: 61.74

GC content: 61.43

Gene sequence:

>1680_bases
ATGATAGAGTGGAATTCCATGGTTAGAAGAACACATGGTAATTCTCAAGAGCACGTCACCAAGCATATTTTCGTCACTGG
CGGCGTTGTTTCCTCTCTCGGCAAGGGCCTGACCGCATCTTCGCTCGGTCGTTTGCTGCGCAGCCGCGGCATCCATGTTC
TGCAGCAAAAGCTCGATCCGTACATCAACGTCGACCCGGGCACCATGAACCCGTTCCAGCATGGTGAGGTCTACGTGACC
GAAGACGGTGCTGAAACCGATCTCGACATCGGCCACTACGAGCGTTTCCTCGACGTCTTCCTGAGCCAGAAGGCCAACGT
CACCACCGGTCAGATCTATCAGGAAGTGCTGCGCAAGGAGCGCGCCGGCGAATACCTCGGCCAGTGCGTGCAGGTCATCC
CTCACATCACCAACGAAATCAAGTCTCGTATGCGCGCCCAGGCTTCCGATGACGTGGATGTGATCATCACCGAGATCGGT
GGTACTGTCGGCGACATCGAGTCCCAGCCCTTCCTTGAGGCCGCCCGCGAAGTGCGCCGCGATCTCGGCCCGGACAACTG
CATGTTCGTGCACGTTTCCCTGGTGCCGTACATTTCCGCCGCCCACGAGCTCAAGACCAAGCCCACCCAGCACTCCGTGA
TGATGCTGCGTCAACTCGGCATCTCCCCGGACGCCCTCGTGTTGCGTTCCGACCGCCCGCTCAACCAGTCCATCAAAGAC
AAGATCTCCCTGATGTGCGACGTCGATGCCGAAGGCGTGGTCAACTGCGTGGATGCGCCGAGCATCTACGATGTACCGAA
GATCCTGTTCGAAGAGGGCCTTGATGCTTACGTGGTGCGTGAGCTCGGCCTGCCGTTCCACGATGTCGATTGGGATGAAT
GGGCAGACCTGCTTGAGCGCGTCCACCACCCCAAGCACGAGGTCAACATCGCCATCGTCGGCAAGTACATCGATTTGCCG
GACGCCTACCTGTCTGTCACCGAGGCCATCAAGGCCGGAGGCTTTGCCAACTGGGCCAAGGTCAACGTCAAGTGGGTTGC
GGCCGACCGTTGCGAAACCACCGAAGGTGCGGCCGCCGCTCTCGACAACGTCGACGGCATCGTGATCCCCGGCGGCTTTG
GCATCCGCGGCATCGATGGCAAGATCGGCGCCCTGAAGTTCGCCCGTGAAACCAAGCTGCCCGCCCTTGGCCTGTGCCTG
GGCCTGCAGTCCATGGTCATCGAATACTCCCGCCACGTGCTGGGCATTGAAGACGCGAACTCTTCCGAGTTCGAGCCCGA
TTGCGCCAACCCGGTGATCGCCACCATGGAAGAGCAGAAGGACATCGTGGCCGGCAAGGGCGACATGGGCCACACCATGC
GTCTTGGCTCCTACCCGGCTGAGCTGGAGGAAGGCTCGCTGGTCGCCGAACTGTACGGCACCACGCATGTCACCGAGCGT
CACCGCCACCGTTACGAAGTCAACGTGGCGTACAAGGATCGTCTGCGCGAAGGCGGCCTGCGCATCTCTGGCCAGAGCCC
GGATGGCGAGCTCACCGAGTTCGTGGAACTGCCGCAGGACGTGCACCCGTTCTATGTGGCCACCCAGGCTCACCCGGAGT
TCAAGTCCCGCCCGACCAAGCCACATCCGCTGTTCGCCGGTCTGGTCAAGGCGGCGCTCGGCCATCAGGCGGCCCGCTGA

Upstream 100 bases:

>100_bases
GCGCCGTGTATTCGACCATATGCTGGCCAAGGCAGGACGAGACGGGCCGCCTACGCGCGACTACACCGGCCACGTCATGA
ACACGCCGGGTCATACACGG

Downstream 100 bases:

>100_bases
CCGTGGATCCCGCGCGGTCGGTCCGCGCACGATGAAGGCGTGACGGCACGGCATCTCAGGATGCTTCGCCGCCACGCCTT
CGCCGTTTGGCGCCATCCGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MIEWNSMVRRTHGNSQEHVTKHIFVTGGVVSSLGKGLTASSLGRLLRSRGIHVLQQKLDPYINVDPGTMNPFQHGEVYVT
EDGAETDLDIGHYERFLDVFLSQKANVTTGQIYQEVLRKERAGEYLGQCVQVIPHITNEIKSRMRAQASDDVDVIITEIG
GTVGDIESQPFLEAAREVRRDLGPDNCMFVHVSLVPYISAAHELKTKPTQHSVMMLRQLGISPDALVLRSDRPLNQSIKD
KISLMCDVDAEGVVNCVDAPSIYDVPKILFEEGLDAYVVRELGLPFHDVDWDEWADLLERVHHPKHEVNIAIVGKYIDLP
DAYLSVTEAIKAGGFANWAKVNVKWVAADRCETTEGAAAALDNVDGIVIPGGFGIRGIDGKIGALKFARETKLPALGLCL
GLQSMVIEYSRHVLGIEDANSSEFEPDCANPVIATMEEQKDIVAGKGDMGHTMRLGSYPAELEEGSLVAELYGTTHVTER
HRHRYEVNVAYKDRLREGGLRISGQSPDGELTEFVELPQDVHPFYVATQAHPEFKSRPTKPHPLFAGLVKAALGHQAAR

Sequences:

>Translated_559_residues
MIEWNSMVRRTHGNSQEHVTKHIFVTGGVVSSLGKGLTASSLGRLLRSRGIHVLQQKLDPYINVDPGTMNPFQHGEVYVT
EDGAETDLDIGHYERFLDVFLSQKANVTTGQIYQEVLRKERAGEYLGQCVQVIPHITNEIKSRMRAQASDDVDVIITEIG
GTVGDIESQPFLEAAREVRRDLGPDNCMFVHVSLVPYISAAHELKTKPTQHSVMMLRQLGISPDALVLRSDRPLNQSIKD
KISLMCDVDAEGVVNCVDAPSIYDVPKILFEEGLDAYVVRELGLPFHDVDWDEWADLLERVHHPKHEVNIAIVGKYIDLP
DAYLSVTEAIKAGGFANWAKVNVKWVAADRCETTEGAAAALDNVDGIVIPGGFGIRGIDGKIGALKFARETKLPALGLCL
GLQSMVIEYSRHVLGIEDANSSEFEPDCANPVIATMEEQKDIVAGKGDMGHTMRLGSYPAELEEGSLVAELYGTTHVTER
HRHRYEVNVAYKDRLREGGLRISGQSPDGELTEFVELPQDVHPFYVATQAHPEFKSRPTKPHPLFAGLVKAALGHQAAR
>Mature_559_residues
MIEWNSMVRRTHGNSQEHVTKHIFVTGGVVSSLGKGLTASSLGRLLRSRGIHVLQQKLDPYINVDPGTMNPFQHGEVYVT
EDGAETDLDIGHYERFLDVFLSQKANVTTGQIYQEVLRKERAGEYLGQCVQVIPHITNEIKSRMRAQASDDVDVIITEIG
GTVGDIESQPFLEAAREVRRDLGPDNCMFVHVSLVPYISAAHELKTKPTQHSVMMLRQLGISPDALVLRSDRPLNQSIKD
KISLMCDVDAEGVVNCVDAPSIYDVPKILFEEGLDAYVVRELGLPFHDVDWDEWADLLERVHHPKHEVNIAIVGKYIDLP
DAYLSVTEAIKAGGFANWAKVNVKWVAADRCETTEGAAAALDNVDGIVIPGGFGIRGIDGKIGALKFARETKLPALGLCL
GLQSMVIEYSRHVLGIEDANSSEFEPDCANPVIATMEEQKDIVAGKGDMGHTMRLGSYPAELEEGSLVAELYGTTHVTER
HRHRYEVNVAYKDRLREGGLRISGQSPDGELTEFVELPQDVHPFYVATQAHPEFKSRPTKPHPLFAGLVKAALGHQAAR

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=46.2365591397849, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=43.8282647584973, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=43.8282647584973, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=43.8282647584973, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=51.39146567718, Blast_Score=550, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI25148299, Length=605, Percent_Identity=36.5289256198347, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319432, Length=566, Percent_Identity=42.5795053003534, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322563, Length=568, Percent_Identity=41.9014084507042, Blast_Score=412, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=44.9016100178891, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=43.452380952381, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 61687; Mature: 61687

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEWNSMVRRTHGNSQEHVTKHIFVTGGVVSSLGKGLTASSLGRLLRSRGIHVLQQKLDP
CCCHHHHHHHHCCCCHHHHEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCC
YINVDPGTMNPFQHGEVYVTEDGAETDLDIGHYERFLDVFLSQKANVTTGQIYQEVLRKE
CEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAGEYLGQCVQVIPHITNEIKSRMRAQASDDVDVIITEIGGTVGDIESQPFLEAAREVRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
DLGPDNCMFVHVSLVPYISAAHELKTKPTQHSVMMLRQLGISPDALVLRSDRPLNQSIKD
HCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KISLMCDVDAEGVVNCVDAPSIYDVPKILFEEGLDAYVVRELGLPFHDVDWDEWADLLER
HHHEEECCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VHHPKHEVNIAIVGKYIDLPDAYLSVTEAIKAGGFANWAKVNVKWVAADRCETTEGAAAA
HCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHH
LDNVDGIVIPGGFGIRGIDGKIGALKFARETKLPALGLCLGLQSMVIEYSRHVLGIEDAN
HCCCCEEEECCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
SSEFEPDCANPVIATMEEQKDIVAGKGDMGHTMRLGSYPAELEEGSLVAELYGTTHVTER
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEHHCCHHHHHH
HRHRYEVNVAYKDRLREGGLRISGQSPDGELTEFVELPQDVHPFYVATQAHPEFKSRPTK
HCCEEEEEEHHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHCCCCC
PHPLFAGLVKAALGHQAAR
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIEWNSMVRRTHGNSQEHVTKHIFVTGGVVSSLGKGLTASSLGRLLRSRGIHVLQQKLDP
CCCHHHHHHHHCCCCHHHHEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCC
YINVDPGTMNPFQHGEVYVTEDGAETDLDIGHYERFLDVFLSQKANVTTGQIYQEVLRKE
CEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAGEYLGQCVQVIPHITNEIKSRMRAQASDDVDVIITEIGGTVGDIESQPFLEAAREVRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
DLGPDNCMFVHVSLVPYISAAHELKTKPTQHSVMMLRQLGISPDALVLRSDRPLNQSIKD
HCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KISLMCDVDAEGVVNCVDAPSIYDVPKILFEEGLDAYVVRELGLPFHDVDWDEWADLLER
HHHEEECCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VHHPKHEVNIAIVGKYIDLPDAYLSVTEAIKAGGFANWAKVNVKWVAADRCETTEGAAAA
HCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHH
LDNVDGIVIPGGFGIRGIDGKIGALKFARETKLPALGLCLGLQSMVIEYSRHVLGIEDAN
HCCCCEEEECCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
SSEFEPDCANPVIATMEEQKDIVAGKGDMGHTMRLGSYPAELEEGSLVAELYGTTHVTER
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEHHCCHHHHHH
HRHRYEVNVAYKDRLREGGLRISGQSPDGELTEFVELPQDVHPFYVATQAHPEFKSRPTK
HCCEEEEEEHHHHHHHHCCEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHCCCCC
PHPLFAGLVKAALGHQAAR
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]